Physiological and Molecular Analysis of the Maturation Process in Fruits of Clementine Mandarin and One of Its Late-Ripening Mutants

Publication Overview
TitlePhysiological and Molecular Analysis of the Maturation Process in Fruits of Clementine Mandarin and One of Its Late-Ripening Mutants
AuthorsDistefano G, Las Casas G, Caruso M, Todaro A, Rapisarda P, La Malfa S, Gentile A, Tribulato E
TypeJournal Article
Journal NameJournal of agricultural and food chemistry
Volume57
Issue17
Year2009
Page(s)7974-7982
CitationDistefano G, Las Casas G, Caruso M, Todaro A, Rapisarda P, La Malfa S, Gentile A, Tribulato E. Physiological and Molecular Analysis of the Maturation Process in Fruits of Clementine Mandarin and One of Its Late-Ripening Mutants. Journal of agricultural and food chemistry. 2009; 57(17):7974-7982.

Abstract

Peel color is one of the main features affecting citrus quality. Clementine is a widespread citrus species with several mutants showing a delay in pigmentation and harvesting. This work characterizes the fruit development and ripening of two clementine clones, 'Comune', a widespread variety, and one of its natural mutations, 'Tardivo', which differ by a delayed color-break and extended harvest period. Morphological, chemical, and molecular analyses were carried out on fruits of both genotypes during the whole maturation process. Analysis showed that mutation did not affect ripening characteristics such as juice acidity and TSS. However, biochemical and molecular analysis revealed marked differences in the flavedo regarding carotenogenesis and chlorophyllase gene expression. Carotenoid showed quantitative differences at biochemical and molecular levels. Results demonstrated that the mutation in 'Tardivo' influenced the transcriptional activation of PSY, a key step in carotenoid biosynthesis. The differential PSY expression led to a significant quantitative difference in phytoene accumulation between the two genotypes. Also, 'Tardivo' showed delayed accumulation of carotenes, lutein, and β,β-xanthophylls. The differential expression of genes involved in ethylene biosynthesis and perception suggested differing responses to ethylene signaling between the two genotypes. Moreover, exogenous application of ethylene revealed a different sensitivity of the two varieties to this hormone. The analysis added new information to better understand the complex process of ripening in citrus.
Stocks
This publication contains information about 2 stocks:
Stock NameUniquenameType
ComuneComunebreeding_research_material
TardivoTardivobreeding_research_material
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2009
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1021/jf900710v
Keywordsclementines, Citrus clementina, ripening, clones, cultivars, fruiting, color, genotype, fruit juices, acidity, gene expression, carotenoids, chlorophyllase, exocarp, biosynthesis, transcriptional activation, carotenes, lutein, xanthophylls, ethylene production, chemical constituents of plants, mutants
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4258840
PMID: PubMedPMID:19655798