CX043577 | Citrus Genome Database

Overview


Name	CX043577
Unique Name	CX043577
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	619

Libraries


Library Name	Type
Parent Washington Navel Orange Thrip-Challenged Flavedo cDNA Library UCRCS07	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig12923	contig	Csv1_Contig12923:481..1098.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CX043577 vs. ExPASy Swiss-Prot
Match: CHI2_PEA (Endochitinase A2 OS=Pisum sativum GN=CHI2 PE=1 SV=2)

HSP 1 Score: 165.236 bits (417), Expect = 3.459e-47
Identity = 75/123 (60.98%), Postives = 95/123 (77.24%), Query Frame = -2

Query:  184 GLGIGEELLNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIEEWKPSANDVNAGRLKGYGLTSNIINGGIECGQGGNAAVRNRIGFFTTFCGKFGIQPGDSLDCYNQRP*GLNLMAQSM 552
            G  IG +LLNNPDL+ATDPV+SFK+A+WFWMT Q PKPSCH+VI   W PS+ D  AGRL GYG  +NIINGG+ECG+G ++ V++RIGF+  +C  FGI  GD+LDCY+QRP G +L   S+
Sbjct:  194 GRAIGVDLLNNPDLVATDPVISFKTALWFWMTPQSPKPSCHDVITGGWTPSSADRAAGRLPGYGTVTNIINGGLECGRGQDSRVQDRIGFYKRYCDIFGIGYGDNLDCYSQRPFGSSLPLSSI 316

HSP 2 Score: 44.2838 bits (103), Expect = 3.459e-47
Identity = 16/20 (80.00%), Postives = 19/20 (95.00%), Query Frame = -1

Query:  557 KYYGRGPIQLSWNYNYLRCG 616
            +YYGRGPIQ+SWNYNY +CG
Sbjct:  175 QYYGRGPIQISWNYNYGQCG 194

[Close]

BLAST of CX043577 vs. ExPASy Swiss-Prot
Match: CHI1_GOSHI (Endochitinase 1 OS=Gossypium hirsutum PE=3 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 3.753e-46
Identity = 73/123 (59.35%), Postives = 91/123 (73.98%), Query Frame = -2

Query:  184 GLGIGEELLNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIEEWKPSANDVNAGRLKGYGLTSNIINGGIECGQGGNAAVRNRIGFFTTFCGKFGIQPGDSLDCYNQRP*GLNLMAQSM 552
            G  IG +LLNNPDLL++DP +SFKSA WFWMT Q PKPSCH VII  W PS++D  AGR+ GYG+ +NIINGG+ECG+G NA V +RIGF+  +C   G+  G++LDCYNQ P G  +   SM
Sbjct:  202 GRAIGVDLLNNPDLLSSDPTISFKSAFWFWMTPQSPKPSCHNVIIGAWSPSSSDRAAGRVPGYGVITNIINGGLECGKGWNAQVEDRIGFYKRYCDILGVSYGNNLDCYNQSPFGNGVSVDSM 324

HSP 2 Score: 42.3578 bits (98), Expect = 3.753e-46
Identity = 15/20 (75.00%), Postives = 19/20 (95.00%), Query Frame = -1

Query:  557 KYYGRGPIQLSWNYNYLRCG 616
            +Y+GRGP+QLSWNYNY +CG
Sbjct:  183 QYFGRGPMQLSWNYNYGQCG 202

[Close]

BLAST of CX043577 vs. ExPASy Swiss-Prot
Match: CHI1_THECC (Endochitinase 1 OS=Theobroma cacao GN=CHIA1 PE=2 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 3.753e-46
Identity = 73/112 (65.18%), Postives = 87/112 (77.68%), Query Frame = -2

Query:  217 GLGIGEELLNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIEEWKPSANDVNAGRLKGYGLTSNIINGGIECGQGGNAAVRNRIGFFTTFCGKFGIQPGDSLDCYNQR 552
            G  IG +LLNNPDLLATDP +SFKSA WFWMT Q PKPSCH+VII  W PS +D  AGR+ G+GL +NIINGG+ECGQG NA V +RIGF+  +C   G+  G++LDCYNQR
Sbjct:  199 GRAIGVDLLNNPDLLATDPTISFKSAFWFWMTPQSPKPSCHDVIIGAWSPSGSDQAAGRVPGFGLITNIINGGLECGQGWNAKVEDRIGFYKRYCDTLGVGYGNNLDCYNQR 310

HSP 2 Score: 41.2022 bits (95), Expect = 3.753e-46
Identity = 14/20 (70.00%), Postives = 19/20 (95.00%), Query Frame = -1

Query:  557 KYYGRGPIQLSWNYNYLRCG 616
            +Y+GRGP+QL+WNYNY +CG
Sbjct:  180 QYFGRGPMQLTWNYNYGQCG 199

[Close]

BLAST of CX043577 vs. ExPASy Swiss-Prot
Match: CHIB_ARATH (Basic endochitinase B OS=Arabidopsis thaliana GN=CHI-B PE=1 SV=3)

HSP 1 Score: 162.54 bits (410), Expect = 4.889e-46
Identity = 71/112 (63.39%), Postives = 88/112 (78.57%), Query Frame = -2

Query:  217 GLGIGEELLNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIEEWKPSANDVNAGRLKGYGLTSNIINGGIECGQGGNAAVRNRIGFFTTFCGKFGIQPGDSLDCYNQR 552
            G  IG +LLNNPDL+A D V++FK+AIWFWMTAQPPKPSCH VI  +W+PS  D  AGRL GYG+ +NIINGG+ECG+G +  V +RIGF+  +C  FG+ PG +LDCYNQR
Sbjct:  213 GRAIGVDLLNNPDLVANDAVIAFKAAIWFWMTAQPPKPSCHAVIAGQWQPSDADRAAGRLPGYGVITNIINGGLECGRGQDGRVADRIGFYQRYCNIFGVNPGGNLDCYNQR 324

HSP 2 Score: 43.1282 bits (100), Expect = 4.889e-46
Identity = 16/20 (80.00%), Postives = 18/20 (90.00%), Query Frame = -1

Query:  557 KYYGRGPIQLSWNYNYLRCG 616
            +YYGRGP+QLSWNYNY  CG
Sbjct:  194 RYYGRGPMQLSWNYNYGLCG 213

[Close]

BLAST of CX043577 vs. ExPASy Swiss-Prot
Match: CHI1_TOBAC (Endochitinase A OS=Nicotiana tabacum GN=CHN48 PE=1 SV=2)

HSP 1 Score: 167.162 bits (422), Expect = 6.372e-46
Identity = 74/123 (60.16%), Postives = 95/123 (77.24%), Query Frame = -2

Query:  184 GLGIGEELLNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIEEWKPSANDVNAGRLKGYGLTSNIINGGIECGQGGNAAVRNRIGFFTTFCGKFGIQPGDSLDCYNQRP*GLNLMAQSM 552
            G  IG +LLNNPDL+ATDPV+SFKSA+WFWMT Q PKPSCH+VII  W+PSA D  A RL G+G+ +NIINGG+ECG+G ++ V++RIGF+  +C   G+ PGD+LDC NQR  G  L+  +M
Sbjct:  207 GRAIGVDLLNNPDLVATDPVISFKSALWFWMTPQSPKPSCHDVIIGRWQPSAGDRAANRLPGFGVITNIINGGLECGRGTDSRVQDRIGFYRRYCSILGVSPGDNLDCGNQRSFGNGLLVDTM 329

HSP 2 Score: 38.1206 bits (87), Expect = 6.372e-46
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = -1

Query:  557 KYYGRGPIQLSWNYNYLRCG 616
            KY+GRGPIQ+S NYNY  CG
Sbjct:  188 KYFGRGPIQISHNYNYGPCG 207

[Close]

BLAST of CX043577 vs. ExPASy Swiss-Prot
Match: CHI2_GOSHI (Endochitinase 2 (Fragment) OS=Gossypium hirsutum PE=2 SV=2)

HSP 1 Score: 162.925 bits (411), Expect = 6.379e-46
Identity = 73/123 (59.35%), Postives = 90/123 (73.17%), Query Frame = -2

Query:  184 GLGIGEELLNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIEEWKPSANDVNAGRLKGYGLTSNIINGGIECGQGGNAAVRNRIGFFTTFCGKFGIQPGDSLDCYNQRP*GLNLMAQSM 552
            G  IG +LLNNPDLL++DP +SFKSA WFWMT Q PKPSCH VII  W PS++D  AGR  GYG+ +NIINGG+ECG+G NA V +RIGF+  +C   G+  G++LDCYNQ P G  +   SM
Sbjct:  180 GRAIGVDLLNNPDLLSSDPTISFKSAFWFWMTPQSPKPSCHNVIIGAWSPSSSDRAAGRATGYGVITNIINGGLECGKGWNAQVEDRIGFYKRYCDILGVSYGNNLDCYNQSPFGNGVSVDSM 302

HSP 2 Score: 42.3578 bits (98), Expect = 6.379e-46
Identity = 15/20 (75.00%), Postives = 19/20 (95.00%), Query Frame = -1

Query:  557 KYYGRGPIQLSWNYNYLRCG 616
            +Y+GRGP+QLSWNYNY +CG
Sbjct:  161 QYFGRGPMQLSWNYNYGQCG 180

[Close]

BLAST of CX043577 vs. ExPASy Swiss-Prot
Match: CHI2_TOBAC (Endochitinase B OS=Nicotiana tabacum GN=CHN50 PE=1 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 2.396e-45
Identity = 73/123 (59.35%), Postives = 95/123 (77.24%), Query Frame = -2

Query:  184 GLGIGEELLNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIEEWKPSANDVNAGRLKGYGLTSNIINGGIECGQGGNAAVRNRIGFFTTFCGKFGIQPGDSLDCYNQRP*GLNLMAQSM 552
            G  IG +LLNNPDL+ATDPV+SFKSA+WFWMT Q PKPSCH+VII  W+PS+ D  A RL G+G+ +NIINGG+ECG+G ++ V++RIGF+  +C   G+ PGD+LDC NQR  G  L+  +M
Sbjct:  202 GRAIGVDLLNNPDLVATDPVISFKSALWFWMTPQSPKPSCHDVIIGRWQPSSADRAANRLPGFGVITNIINGGLECGRGTDSRVQDRIGFYRRYCSILGVSPGDNLDCGNQRSFGNGLLVDTM 324

HSP 2 Score: 38.1206 bits (87), Expect = 2.396e-45
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = -1

Query:  557 KYYGRGPIQLSWNYNYLRCG 616
            KY+GRGPIQ+S NYNY  CG
Sbjct:  183 KYFGRGPIQISHNYNYGPCG 202

[Close]

BLAST of CX043577 vs. ExPASy Swiss-Prot
Match: CHI2_BRANA (Endochitinase CH25 OS=Brassica napus PE=2 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 2.396e-45
Identity = 68/112 (60.71%), Postives = 88/112 (78.57%), Query Frame = -2

Query:  217 GLGIGEELLNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIEEWKPSANDVNAGRLKGYGLTSNIINGGIECGQGGNAAVRNRIGFFTTFCGKFGIQPGDSLDCYNQR 552
            G  IG +LLNNPDL++ DPV++FK+AIWFWMT Q PKPSCH VI+ +W+PS  D  AGR+ GYG+ +NIINGG+ECG+G +A V +RIGF+  +C   G+ PG +LDCYNQR
Sbjct:  198 GRAIGSDLLNNPDLVSNDPVIAFKAAIWFWMTPQSPKPSCHAVIVGQWQPSDADRAAGRVPGYGVITNIINGGLECGRGQDARVADRIGFYQRYCNILGVNPGGNLDCYNQR 309

HSP 2 Score: 43.5134 bits (101), Expect = 2.396e-45
Identity = 16/19 (84.21%), Postives = 18/19 (94.74%), Query Frame = -1

Query:  557 YYGRGPIQLSWNYNYLRCG 613
            YYGRGP+QLSWNYNY +CG
Sbjct:  180 YYGRGPMQLSWNYNYGQCG 198

[Close]

BLAST of CX043577 vs. ExPASy Swiss-Prot
Match: CHI3_TOBAC (Endochitinase 3 OS=Nicotiana tabacum GN=CHN14 PE=3 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 4.068e-45
Identity = 72/123 (58.54%), Postives = 92/123 (74.80%), Query Frame = -2

Query:  184 GLGIGEELLNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIEEWKPSANDVNAGRLKGYGLTSNIINGGIECGQGGNAAVRNRIGFFTTFCGKFGIQPGDSLDCYNQRP*GLNLMAQSM 552
            G  IG+ LLNNPDL+AT+ V+SFKSAIWFWMTAQ PKPSCH+VI   W PSA D  A RL GYG+ +NIINGG+ECG G +A V++RIGF+  +C   G+ PGD++DC NQ+     L+ ++M
Sbjct:  212 GRAIGQNLLNNPDLVATNAVVSFKSAIWFWMTAQSPKPSCHDVITGRWTPSAADRAANRLPGYGVITNIINGGLECGHGSDARVQDRIGFYRRYCSILGVSPGDNIDCGNQKSFNSGLLLETM 334

HSP 2 Score: 41.2022 bits (95), Expect = 4.068e-45
Identity = 16/20 (80.00%), Postives = 18/20 (90.00%), Query Frame = -1

Query:  557 KYYGRGPIQLSWNYNYLRCG 616
            KYYGRGPIQ+S+NYNY  CG
Sbjct:  193 KYYGRGPIQISYNYNYGPCG 212

[Close]

BLAST of CX043577 vs. ExPASy Swiss-Prot
Match: CHIT_CARPA (Endochitinase OS=Carica papaya PE=1 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 5.325e-45
Identity = 68/115 (59.13%), Postives = 87/115 (75.65%), Query Frame = -2

Query:  208 GLGIGEELLNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIEEWKPSANDVNAGRLKGYGLTSNIINGGIECGQGGNAAVRNRIGFFTTFCGKFGIQPGDSLDCYNQRP*G 552
            G  +   LL NPDL+ATD VLSFK+A+WFWMT Q PKPSCH+V+   W+PSA D  AGRL GYG+ +N++NGG+ECG+G N  V +R+GFF  +CG  G+  G++LDCYNQRP G
Sbjct:  129 GEALRVNLLGNPDLVATDRVLSFKTALWFWMTPQAPKPSCHDVLTGRWQPSAADTAAGRLPGYGVLTNLLNGGLECGKGPNPQVADRLGFFRRYCGLLGVGTGNNLDCYNQRPFG 243

HSP 2 Score: 45.0542 bits (105), Expect = 5.325e-45
Identity = 18/23 (78.26%), Postives = 19/23 (82.61%), Query Frame = -1

Query:  545 YYGRGPIQLSWNYNYLRCGERPR 613
            YYGRGP+QLSWNYNY  CGE  R
Sbjct:  111 YYGRGPLQLSWNYNYGPCGEALR 133

The following BLAST results are available for this feature:

BLAST of CX043577 vs. ExPASy Swiss-Prot

BLAST of CX043577 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 55


Match Name	E-value	Identity	Description
CHI2_PEA	3.459e-47	60.98	Endochitinase A2 OS=Pisum sativum GN=CHI2 PE=1 SV=...	[more]
CHI1_GOSHI	3.753e-46	59.35	Endochitinase 1 OS=Gossypium hirsutum PE=3 SV=1	[more]
CHI1_THECC	3.753e-46	65.18	Endochitinase 1 OS=Theobroma cacao GN=CHIA1 PE=2 S...	[more]
CHIB_ARATH	4.889e-46	63.39	Basic endochitinase B OS=Arabidopsis thaliana GN=C...	[more]
CHI1_TOBAC	6.372e-46	60.16	Endochitinase A OS=Nicotiana tabacum GN=CHN48 PE=1...	[more]
CHI2_GOSHI	6.379e-46	59.35	Endochitinase 2 (Fragment) OS=Gossypium hirsutum P...	[more]
CHI2_TOBAC	2.396e-45	59.35	Endochitinase B OS=Nicotiana tabacum GN=CHN50 PE=1...	[more]
CHI2_BRANA	2.396e-45	60.71	Endochitinase CH25 OS=Brassica napus PE=2 SV=1	[more]
CHI3_TOBAC	4.068e-45	58.54	Endochitinase 3 OS=Nicotiana tabacum GN=CHN14 PE=3...	[more]
CHIT_CARPA	5.325e-45	59.13	Endochitinase OS=Carica papaya PE=1 SV=1	[more]

Pages

Properties


Property Name	Value
Genbank description	UCRCS07_12A09_b Parent Washington Navel Orange Thrip-Challenged Flavedo cDNA Library UCRCS07 Citrus sinensis cDNA clone UCRCS07-12A09-A18-1-6.b, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CX043577 ID=CX043577; Name=CX043577; organism=Citrus sinensis; type=EST; length=619bp
CCCCCCCTTTTTTTGATGGTGAAGACTCATCGATCCATGTTGGATCAGTC
TTATTTAATTTACTTACAATAAGAGCGTAACAAACTAACAATTTAGTTGA
CCACCTACAAGTGTGACATCTAAAGTTTTTATTAAGTAGCTTTTATTAAG
CTTATTTTACTAAAACGTTGATCATGATGATCACATAGATTGTGCCATCA
GATTAAGGCCTTAAGGTCGTTGGTTGTAGCAATCAAGACTGTCACCAGGC
TGAATCCCAAACTTGCCACAGAAAGTGGTAAAGAACCCAATACGGTTACG
TACAGCGGCATTCCCACCTTGGCCACATTCAATTCCACCGTTGATTATAT
TTGAGGTTAGACCGTATCCTTTAAGCCGGCCGGCGTTTACGTCATTTGCT
GATGGTTTCCATTCTTCGATAATGACTTCGTGGCACGATGGCTTTGGTGG
CTGTGCAGTCATCCAGAACCAGATTGCTGACTTGAATGATAGTACTGGAT
CAGTAGCAAGAAGGTCTGGATTGTTCAACAACTCCTCTCCTATCCCTAGG
CCTTTCTCCGCACCGTAAGTAATTGTAATTCCAAGAGAGTTGAATTGGAC
CTCGGCCATAGTACTTCCC