EY676451 | Citrus Genome Database

Overview


Name	EY676451
Unique Name	EY676451
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	904

Libraries


Library Name	Type
Sweet orange leaf, infected with CiLV	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY676451 vs. ExPASy Swiss-Prot
Match: LGUL_ORYSJ (Lactoylglutathione lyase OS=Oryza sativa subsp. japonica GN=GLX-I PE=1 SV=2)

HSP 1 Score: 211.075 bits (536), Expect = 7.200e-54
Identity = 111/200 (55.50%), Postives = 136/200 (68.00%), Query Frame = 2

Query:    2 DKYDIRTGFGHFGIAVEDVAKTVDLVKAKGG-KVTREPGPVKGGNTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGDLDRAINFYKKAFGMELLRKRDNPDYKYTIAVMGYGPEDKNAVLELTYNHGVTEY*QRKWLRSDCNRHK*CLQDS*SNQ---TEWGKDYPLNLGPCLVSTQKLLLAWNPDGWKSVFVENPD 589
            DKYDI  GFGHF IA EDV K  + +K+    K+TREPGPVKGG+TVIAF +DPDGY FEL++RGPTPEPLCQVMLRVGDLDR+I FY+KA GM+LLRK+D PDYKYTIA++GY  EDK  V+ELTYN+GVTEY +           +   + + + +    E G       GP      K+    +PDGWK V V+N D
Sbjct:   86 DKYDIGAGFGHFAIATEDVYKLAEKIKSSCCCKITREPGPVKGGSTVIAFAQDPDGYMFELIQRGPTPEPLCQVMLRVGDLDRSIKFYEKALGMKLLRKKDVPDYKYTIAMLGYADEDKTTVIELTYNYGVTEYTKGNAYAQVAIGTEDVYKSAEAVELVTKELGGKILRQPGPLPGLNTKIASFLDPDGWKVVLVDNAD 285

HSP 2 Score: 83.1889 bits (204), Expect = 2.264e-15
Identity = 39/64 (60.94%), Postives = 47/64 (73.44%), Query Frame = 2

Query:  209 LCQVMLRVGDLDRAINFYKKAFGMELLRKRDNPDYKYTIAVMGYGPEDKNAVLELTYNHGVTEY 400
            L   + RVGDLDR I  Y + FGM+LLRKRD P+ KYT A +G+GPED N  LELTYN+GV +Y
Sbjct:   25 LLHAVYRVGDLDRTIKCYTECFGMKLLRKRDVPEEKYTNAFLGFGPEDTNFALELTYNYGVDKY 88

[Close]

BLAST of EY676451 vs. ExPASy Swiss-Prot
Match: LGUL_BRAOG (Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera PE=2 SV=1)

HSP 1 Score: 201.83 bits (512), Expect = 4.368e-51
Identity = 108/198 (54.55%), Postives = 134/198 (67.68%), Query Frame = 2

Query:    8 YDIRTGFGHFGIAVEDVAKTVDLVKAKGGKVTREPGPVKGGNTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGDLDRAINFYKKAFGMELLRKRDNPDYKYTIAVMGYGPEDKNAVLELTYNHGVTEY*QRKWLRSDC----NRHK*CLQDS*SNQTEWGKDYPLNLGPCLVSTQKLLLAWNPDGWKSVFVENPD 589
            YDI TGFGHF I+ +DV+K V+ V+AKGG VTREPGPVKGG +VIAF++DPDGY FEL++RGPTPEPLCQVMLRVGDLDRA+ F +KA GM LLR+ + P+Y  TI +MGY  E ++ VLELTYN+GVTEY +            + +K        NQ E G       GP      K++   +PDGWK V V+N D
Sbjct:   81 YDIGTGFGHFAISTQDVSKMVEAVRAKGGNVTREPGPVKGGGSVIAFVKDPDGYTFELIQRGPTPEPLCQVMLRVGDLDRAVKFMEKALGMRLLRRIERPEYN-TIGMMGYAEEYESIVLELTYNYGVTEYTKGNAYAQIAIGTDDVYKSAEVVKIVNQ-ELGGKITREAGPLPGLGTKIVSFLDPDGWKQVLVDNED 276

HSP 2 Score: 82.4185 bits (202), Expect = 3.862e-15
Identity = 37/61 (60.66%), Postives = 48/61 (78.69%), Query Frame = 2

Query:  218 VMLRVGDLDRAINFYKKAFGMELLRKRDNPDYKYTIAVMGYGPEDKNAVLELTYNHGVTEY 400
            V+ RVGDLDR I FY + FGM++LRKRD P+ KY+ A +G+GPE  N V+ELTYN+GV+ Y
Sbjct:   21 VVYRVGDLDRTIQFYTECFGMKVLRKRDVPEEKYSNAFLGFGPETSNFVVELTYNYGVSSY 81

[Close]

BLAST of EY676451 vs. ExPASy Swiss-Prot
Match: LGUL_VIBCH (Probable lactoylglutathione lyase OS=Vibrio cholerae GN=gloA PE=3 SV=2)

HSP 1 Score: 84.7297 bits (208), Expect = 6.899e-22
Identity = 37/64 (57.81%), Postives = 48/64 (75.00%), Query Frame = 2

Query:  209 LCQVMLRVGDLDRAINFYKKAFGMELLRKRDNPDYKYTIAVMGYGPEDKNAVLELTYNHGVTEY 400
            +   MLRVGDLD++I FY +  GM LLRK +N +YKYT+A +GYG E + AV+ELTYN GV +Y
Sbjct:    6 ILHTMLRVGDLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIELTYNWGVADY 69

HSP 2 Score: 41.2022 bits (95), Expect = 6.899e-22
Identity = 17/32 (53.12%), Postives = 22/32 (68.75%), Query Frame = 3

Query:  402 DKGNGYAQIAIGTNDVYKTAEAIKLNGGKITR 497
            +KGN Y  IAIG +D+Y T + IK  GG +TR
Sbjct:   70 EKGNAYGHIAIGVDDIYATCDTIKAAGGIVTR 101

HSP 3 Score: 77.7962 bits (190), Expect = 9.514e-14
Identity = 34/60 (56.67%), Postives = 43/60 (71.67%), Query Frame = 2

Query:    8 YDIRTGFGHFGIAVEDVAKTVDLVKAKGGKVTREPGPVKGGNTVIAFIEDPDGYKFELLE 187
            Y+    +GH  I V+D+  T D +KA GG VTREPGPVKGG T IAF++DPDGY  EL++
Sbjct:   69 YEKGNAYGHIAIGVDDIYATCDTIKAAGGIVTREPGPVKGGTTHIAFVKDPDGYMIELIQ 128

[Close]

BLAST of EY676451 vs. ExPASy Swiss-Prot
Match: LGUL_VIBPA (Probable lactoylglutathione lyase OS=Vibrio parahaemolyticus GN=gloA PE=3 SV=2)

HSP 1 Score: 82.0333 bits (201), Expect = 2.112e-20
Identity = 36/64 (56.25%), Postives = 48/64 (75.00%), Query Frame = 2

Query:  209 LCQVMLRVGDLDRAINFYKKAFGMELLRKRDNPDYKYTIAVMGYGPEDKNAVLELTYNHGVTEY 400
            +   MLRVGDLD++I FY +  GM+LLR  +N +Y+YT+A +GYG E + AV+ELTYN G TEY
Sbjct:    6 ILHTMLRVGDLDKSIKFYTEVMGMQLLRTNENKEYEYTLAFVGYGDESQGAVIELTYNWGKTEY 69

HSP 2 Score: 38.891 bits (89), Expect = 2.112e-20
Identity = 16/32 (50.00%), Postives = 21/32 (65.62%), Query Frame = 3

Query:  402 DKGNGYAQIAIGTNDVYKTAEAIKLNGGKITR 497
            D G  +  IAIG +D+Y T +AIK  GG +TR
Sbjct:   70 DLGTAFGHIAIGVDDIYATCDAIKAAGGNVTR 101

HSP 3 Score: 82.8037 bits (203), Expect = 2.957e-15
Identity = 36/61 (59.02%), Postives = 45/61 (73.77%), Query Frame = 2

Query:    5 KYDIRTGFGHFGIAVEDVAKTVDLVKAKGGKVTREPGPVKGGNTVIAFIEDPDGYKFELLE 187
            +YD+ T FGH  I V+D+  T D +KA GG VTRE GPVKGG T IAF++DPDGY  EL++
Sbjct:   68 EYDLGTAFGHIAIGVDDIYATCDAIKAAGGNVTREAGPVKGGTTHIAFVKDPDGYMIELIQ 128

[Close]

BLAST of EY676451 vs. ExPASy Swiss-Prot
Match: LGUL_SYNY3 (Probable lactoylglutathione lyase OS=Synechocystis sp. (strain PCC 6803) GN=gloA PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 3.140e-18
Identity = 33/64 (51.56%), Postives = 46/64 (71.88%), Query Frame = 2

Query:  209 LCQVMLRVGDLDRAINFYKKAFGMELLRKRDNPDYKYTIAVMGYGPEDKNAVLELTYNHGVTEY 400
            L   M+RVGDLD+++ FY    GM LLRK+D P  ++T+A +GYG E +NAV+ELT+N G  +Y
Sbjct:    3 LLHTMIRVGDLDKSLQFYCDILGMNLLRKKDYPSGEFTLAFVGYGKESENAVIELTHNWGTDKY 66

HSP 2 Score: 39.2762 bits (90), Expect = 3.140e-18
Identity = 15/32 (46.88%), Postives = 21/32 (65.62%), Query Frame = 3

Query:  402 DKGNGYAQIAIGTNDVYKTAEAIKLNGGKITR 497
            D GNG+  IA+G  D+Y T + I+  GGK+ R
Sbjct:   67 DLGNGFGHIALGVEDIYSTCDKIRDKGGKVVR 98

HSP 3 Score: 91.6633 bits (226), Expect = 6.367e-18
Identity = 39/62 (62.90%), Postives = 48/62 (77.42%), Query Frame = 2

Query:    2 DKYDIRTGFGHFGIAVEDVAKTVDLVKAKGGKVTREPGPVKGGNTVIAFIEDPDGYKFELLE 187
            DKYD+  GFGH  + VED+  T D ++ KGGKV REPGP+K G TVIAF+EDPDGYK EL++
Sbjct:   64 DKYDLGNGFGHIALGVEDIYSTCDKIRDKGGKVVREPGPMKHGTTVIAFVEDPDGYKIELIQ 125

[Close]

BLAST of EY676451 vs. ExPASy Swiss-Prot
Match: LGUL_SHIFL (Lactoylglutathione lyase OS=Shigella flexneri GN=gloA PE=3 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.852e-17
Identity = 53/135 (39.26%), Postives = 72/135 (53.33%), Query Frame = 2

Query:  209 LCQVMLRVGDLDRAINFYKKAFGMELLRKRDNPDYKYTIAVMGYGPEDKNAVLELTYNHGVTEY*QRKWLRSDCNRHK*CLQDS*SNQTEW----GKDYPLNLGPCLVSTQKLLLAWNPDGWKSVFVENPDLSQG 601
            L   MLRVGDL R+I+FY K  GM+LLR  +NP+YKY++A +GYGPE + AV+ELTYN GV +Y            H     D+ +   E     G +     GP    T  +    +PDG+K   +E  D  +G
Sbjct:    3 LLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKY-----ELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIEEKDAGRG 132

HSP 2 Score: 84.7297 bits (208), Expect = 7.783e-16
Identity = 35/62 (56.45%), Postives = 48/62 (77.42%), Query Frame = 2

Query:    2 DKYDIRTGFGHFGIAVEDVAKTVDLVKAKGGKVTREPGPVKGGNTVIAFIEDPDGYKFELLE 187
            DKY++ T +GH  ++V++ A+  + ++  GG VTRE GPVKGG TVIAF+EDPDGYK EL+E
Sbjct:   64 DKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIE 125

[Close]

BLAST of EY676451 vs. ExPASy Swiss-Prot
Match: LGUL_ECOLI (Lactoylglutathione lyase OS=Escherichia coli (strain K12) GN=gloA PE=1 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.852e-17
Identity = 53/135 (39.26%), Postives = 72/135 (53.33%), Query Frame = 2

Query:  209 LCQVMLRVGDLDRAINFYKKAFGMELLRKRDNPDYKYTIAVMGYGPEDKNAVLELTYNHGVTEY*QRKWLRSDCNRHK*CLQDS*SNQTEW----GKDYPLNLGPCLVSTQKLLLAWNPDGWKSVFVENPDLSQG 601
            L   MLRVGDL R+I+FY K  GM+LLR  +NP+YKY++A +GYGPE + AV+ELTYN GV +Y            H     D+ +   E     G +     GP    T  +    +PDG+K   +E  D  +G
Sbjct:    3 LLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKY-----ELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIEEKDAGRG 132

HSP 2 Score: 84.7297 bits (208), Expect = 7.783e-16
Identity = 35/62 (56.45%), Postives = 48/62 (77.42%), Query Frame = 2

Query:    2 DKYDIRTGFGHFGIAVEDVAKTVDLVKAKGGKVTREPGPVKGGNTVIAFIEDPDGYKFELLE 187
            DKY++ T +GH  ++V++ A+  + ++  GG VTRE GPVKGG TVIAF+EDPDGYK EL+E
Sbjct:   64 DKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIE 125

[Close]

BLAST of EY676451 vs. ExPASy Swiss-Prot
Match: LGUL_ECO57 (Lactoylglutathione lyase OS=Escherichia coli O157:H7 GN=gloA PE=3 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.852e-17
Identity = 53/135 (39.26%), Postives = 72/135 (53.33%), Query Frame = 2

Query:  209 LCQVMLRVGDLDRAINFYKKAFGMELLRKRDNPDYKYTIAVMGYGPEDKNAVLELTYNHGVTEY*QRKWLRSDCNRHK*CLQDS*SNQTEW----GKDYPLNLGPCLVSTQKLLLAWNPDGWKSVFVENPDLSQG 601
            L   MLRVGDL R+I+FY K  GM+LLR  +NP+YKY++A +GYGPE + AV+ELTYN GV +Y            H     D+ +   E     G +     GP    T  +    +PDG+K   +E  D  +G
Sbjct:    3 LLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKY-----ELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIEEKDAGRG 132

HSP 2 Score: 84.7297 bits (208), Expect = 7.783e-16
Identity = 35/62 (56.45%), Postives = 48/62 (77.42%), Query Frame = 2

Query:    2 DKYDIRTGFGHFGIAVEDVAKTVDLVKAKGGKVTREPGPVKGGNTVIAFIEDPDGYKFELLE 187
            DKY++ T +GH  ++V++ A+  + ++  GG VTRE GPVKGG TVIAF+EDPDGYK EL+E
Sbjct:   64 DKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIE 125

[Close]

BLAST of EY676451 vs. ExPASy Swiss-Prot
Match: LGUL_HAEIN (Lactoylglutathione lyase OS=Haemophilus influenzae GN=gloA PE=1 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 9.193e-17
Identity = 40/70 (57.14%), Postives = 49/70 (70.00%), Query Frame = 2

Query:    2 DKYDIRTGFGHFGIAVEDVAKTVDLVKAKGGKVTREPGPVKGGNTVIAFIEDPDGYKFELLERGPTPEPL 211
            DKY+  T +GH  I V+D+  T + V+A GG VTRE GPVKGG+TVIAF+EDPDGYK E +E   T   L
Sbjct:   64 DKYEHGTAYGHIAIGVDDIYATCEAVRASGGNVTREAGPVKGGSTVIAFVEDPDGYKIEFIENKSTKSGL 133

HSP 2 Score: 78.1814 bits (191), Expect = 7.284e-14
Identity = 49/135 (36.30%), Postives = 65/135 (48.15%), Query Frame = 2

Query:  209 LCQVMLRVGDLDRAINFYKKAFGMELLRKRDNPDYKYTIAVMGYGPEDKNAVLELTYNHGVTEY*QRKWLRSDCNRHK*CLQDS*SNQTE----WGKDYPLNLGPCLVSTQKLLLAWNPDGWKSVFVENPDLSQG 601
            +   MLRVGDLDR+I FY+   GM LLR  +NP+YKYT+A +GY   +  A +ELTYN GV +Y            H     D      E     G +     GP    +  +    +PDG+K  F+EN     G
Sbjct:    3 ILHTMLRVGDLDRSIKFYQDVLGMRLLRTSENPEYKYTLAFLGYEDGESAAEIELTYNWGVDKY-----EHGTAYGHIAIGVDDIYATCEAVRASGGNVTREAGPVKGGSTVIAFVEDPDGYKIEFIENKSTKSG 132

[Close]

BLAST of EY676451 vs. ExPASy Swiss-Prot
Match: LGUL_SALTY (Lactoylglutathione lyase OS=Salmonella typhimurium GN=gloA PE=3 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 5.959e-16
Identity = 51/135 (37.78%), Postives = 70/135 (51.85%), Query Frame = 2

Query:  209 LCQVMLRVGDLDRAINFYKKAFGMELLRKRDNPDYKYTIAVMGYGPEDKNAVLELTYNHGVTEY*QRKWLRSDCNRHK*CLQDS*SNQTEW----GKDYPLNLGPCLVSTQKLLLAWNPDGWKSVFVENPDLSQG 601
            L   MLRVGDL R+I FY    GM+LLR  +NP+YKY++A +GYGPE + AV+ELTYN GV  Y        +   H     D+ +   E     G +     GP    +  +    +PDG+K   +E  D  +G
Sbjct:    3 LLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESYDM-----GNAYGHIALSVDNAAEACERIRQNGGNVTREAGPVKGGSTIIAFVEDPDGYKIELIEAKDAGRG 132

HSP 2 Score: 80.4925 bits (197), Expect = 1.468e-14
Identity = 32/62 (51.61%), Postives = 47/62 (75.81%), Query Frame = 2

Query:    2 DKYDIRTGFGHFGIAVEDVAKTVDLVKAKGGKVTREPGPVKGGNTVIAFIEDPDGYKFELLE 187
            + YD+   +GH  ++V++ A+  + ++  GG VTRE GPVKGG+T+IAF+EDPDGYK EL+E
Sbjct:   64 ESYDMGNAYGHIALSVDNAAEACERIRQNGGNVTREAGPVKGGSTIIAFVEDPDGYKIELIE 125

The following BLAST results are available for this feature:

BLAST of EY676451 vs. ExPASy Swiss-Prot

BLAST of EY676451 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 14


Match Name	E-value	Identity	Description
LGUL_ORYSJ	7.200e-54	55.50	Lactoylglutathione lyase OS=Oryza sativa subsp. ja...	[more]
LGUL_BRAOG	4.368e-51	54.55	Putative lactoylglutathione lyase OS=Brassica oler...	[more]
LGUL_VIBCH	6.899e-22	57.81	Probable lactoylglutathione lyase OS=Vibrio choler...	[more]
LGUL_VIBPA	2.112e-20	56.25	Probable lactoylglutathione lyase OS=Vibrio paraha...	[more]
LGUL_SYNY3	3.140e-18	51.56	Probable lactoylglutathione lyase OS=Synechocystis...	[more]
LGUL_SHIFL	1.852e-17	39.26	Lactoylglutathione lyase OS=Shigella flexneri GN=g...	[more]
LGUL_ECOLI	1.852e-17	39.26	Lactoylglutathione lyase OS=Escherichia coli (stra...	[more]
LGUL_ECO57	1.852e-17	39.26	Lactoylglutathione lyase OS=Escherichia coli O157:...	[more]
LGUL_HAEIN	9.193e-17	57.14	Lactoylglutathione lyase OS=Haemophilus influenzae...	[more]
LGUL_SALTY	5.959e-16	37.78	Lactoylglutathione lyase OS=Salmonella typhimurium...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C1-401-022-G11-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY676451 ID=EY676451; Name=EY676451; organism=Citrus sinensis; type=EST; length=904bp
CGATAAATATGACATCCGAACTGGATTTGGCCATTTTGGCATTGCAGTGG
AGGATGTTGCAAAAACAGTAGATCTTGTAAAGGCAAAAGGGGGAAAAGTG
ACCAGGGAACCTGGTCCTGTTAAAGGTGGCAATACCGTCATTGCTTTTAT
TGAAGATCCAGATGGTTACAAGTTTGAGCTTTTGGAGAGAGGGCCAACAC
CTGAGCCCCTATGTCAAGTAATGCTTAGAGTGGGTGATCTTGATCGTGCT
ATAAATTTCTACAAGAAGGCTTTTGGCATGGAACTTCTGCGGAAAAGAGA
TAACCCTGATTACAAGTATACAATAGCTGTGATGGGCTATGGTCCTGAAG
ATAAAAATGCTGTGCTGGAACTGACATATAACCATGGAGTCACAGAATAT
TGACAAAGGAAATGGTTACGCTCAGATTGCAATAGGCACAAATGATGTTT
ACAAGACAGCTGAAGCAATCAAACTGAATGGGGGAAAGATTACCCGTTGA
ACCTGGGCCCCTGCCTGGTATCAACACAAAAATTACTGCTTGCTTGGAAT
CCTGATGGCTGGAAATCTGTCTTTGTCGAAAATCCTGACCTTTCCCAAGG
ACCCTAAAATTGACCAAAAAATCCTTTGAACCCCCCCCCCCCCTTTTGTT
TAATCCTAAAAGGATTCCTGAAAAATTTTTTCCAATAAAAAAATTCCCCT
CCGCAATAATTTTTTTTTTTTTATATCCCCCCCCGGAAAAAAAATGGCCA
AAAACCCCAAATTTTTAAAAAATTCTTTTTTCTGTAAAAAAAAATTTGTT
ATAACATATTTTCTATCACTTTTTTATGCACAAAAAAATAAAAAAAAATA
AAGGGGGGGGCCCCGCCTCAAAGAAGAAATACCCAAGCTCTTACTCCCCC
CCGC