EY756844

Overview
NameEY756844
Unique NameEY756844
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length702
Libraries
Library NameType
Sweet orange leaf, field plant Bcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1684 contig Csv1_Contig1684:1119..1803. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY756844 vs. ExPASy Swiss-Prot
Match: TCMO_GLYEC (Trans-cinnamate 4-monooxygenase OS=Glycyrrhiza echinata GN=CYP73A14 PE=2 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 3.674e-58
Identity = 63/85 (74.12%), Postives = 65/85 (76.47%), Query Frame = 3 
Query:  192 FWRRESKVEANGNDFRYLPFGVERKSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKIDTAEKGGQVSLHILKHSTIVAKPRSF 446
            F   ES VEANGNDFRYLPFGV R+SCP              RLVQNFELLPPPGQSK+DTAEKGGQ SLHILKHSTIVAKPRSF
Sbjct:  421 FLEEESHVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKLDTAEKGGQFSLHILKHSTIVAKPRSF 505

HSP 2 Score: 112.849 bits (281), Expect = 3.674e-58
Identity = 51/55 (92.73%), Postives = 53/55 (96.36%), Query Frame = 1 
Query:    7 VRKETLRLRMAIPLLVPHMNLHDAKLGGYDVPAESKILVNAWWLANNPAQWKKPE 171
            V KETLRLRMAIPLLVPHMNLHDAKLGGYD+PAESKILVNAWWLANNPA WK+PE
Sbjct:  360 VIKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKRPE 414

HSP 3 Score: 27.335 bits (59), Expect = 3.674e-58
Identity = 12/18 (66.67%), Postives = 12/18 (66.67%), Query Frame = 2 
Query:  149 PPNGKNLNEFTPERFLEE 202
            P N K   EF PERFLEE
Sbjct:  407 PANWKRPEEFRPERFLEE 424
[Close]
BLAST of EY756844 vs. ExPASy Swiss-Prot
Match: TCMO_POPTM (Trans-cinnamate 4-monooxygenase OS=Populus tremuloides GN=CYP73A13 PE=2 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 6.225e-58
Identity = 63/85 (74.12%), Postives = 66/85 (77.65%), Query Frame = 3 
Query:  192 FWRRESKVEANGNDFRYLPFGVERKSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKIDTAEKGGQVSLHILKHSTIVAKPRSF 446
            F   E+KVEANGNDFRYLPFGV R+SCP              RLVQNFELLPPPGQSKIDT+EKGGQ SLHILKHSTIVAKPRSF
Sbjct:  421 FLEEEAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKIDTSEKGGQFSLHILKHSTIVAKPRSF 505

HSP 2 Score: 110.538 bits (275), Expect = 6.225e-58
Identity = 50/55 (90.91%), Postives = 52/55 (94.55%), Query Frame = 1 
Query:    7 VRKETLRLRMAIPLLVPHMNLHDAKLGGYDVPAESKILVNAWWLANNPAQWKKPE 171
            V KETLRLRMAIPLLVPHMNLHDAKLGG+D+PAESKILVNAWWLANNPA WK PE
Sbjct:  360 VVKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPAHWKNPE 414

HSP 3 Score: 28.1054 bits (61), Expect = 6.225e-58
Identity = 12/21 (57.14%), Postives = 14/21 (66.67%), Query Frame = 2 
Query:  149 PPNGKNLNEFTPERFLEERVQ 211
            P + KN  EF PERFLEE  +
Sbjct:  407 PAHWKNPEEFRPERFLEEEAK 427
[Close]
BLAST of EY756844 vs. ExPASy Swiss-Prot
Match: TCMO_HELTU (Trans-cinnamate 4-monooxygenase OS=Helianthus tuberosus GN=CYP73A1 PE=1 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 3.027e-57
Identity = 63/85 (74.12%), Postives = 65/85 (76.47%), Query Frame = 3 
Query:  192 FWRRESKVEANGNDFRYLPFGVERKSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKIDTAEKGGQVSLHILKHSTIVAKPRSF 446
            F   E+KVEANGNDFRYLPFGV R+SCP              RLVQNFELLPPPGQSKIDT EKGGQ SLHILKHSTIVAKPRSF
Sbjct:  421 FLEEEAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKIDTDEKGGQFSLHILKHSTIVAKPRSF 505

HSP 2 Score: 112.079 bits (279), Expect = 3.027e-57
Identity = 51/55 (92.73%), Postives = 53/55 (96.36%), Query Frame = 1 
Query:    7 VRKETLRLRMAIPLLVPHMNLHDAKLGGYDVPAESKILVNAWWLANNPAQWKKPE 171
            V KETLRLRMAIPLLVPHMNLHDAKLGG+D+PAESKILVNAWWLANNP QWKKPE
Sbjct:  360 VVKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPDQWKKPE 414

HSP 3 Score: 25.409 bits (54), Expect = 3.027e-57
Identity = 11/21 (52.38%), Postives = 12/21 (57.14%), Query Frame = 2 
Query:  149 PPNGKNLNEFTPERFLEERVQ 211
            P   K   EF PERFLEE  +
Sbjct:  407 PDQWKKPEEFRPERFLEEEAK 427
[Close]
BLAST of EY756844 vs. ExPASy Swiss-Prot
Match: TCMO_CICAR (Trans-cinnamate 4-monooxygenase OS=Cicer arietinum GN=CYP73A19 PE=2 SV=2)

HSP 1 Score: 125.946 bits (315), Expect = 5.128e-57
Identity = 63/85 (74.12%), Postives = 64/85 (75.29%), Query Frame = 3 
Query:  192 FWRRESKVEANGNDFRYLPFGVERKSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKIDTAEKGGQVSLHILKHSTIVAKPRSF 446
            F   ES VEANGNDFRYLPFGV R+SCP              RLVQNFELLPPPGQSKIDTAEKGGQ SLHILKHSTIV KPRSF
Sbjct:  420 FLEEESHVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKIDTAEKGGQFSLHILKHSTIVCKPRSF 504

HSP 2 Score: 112.079 bits (279), Expect = 5.128e-57
Identity = 51/55 (92.73%), Postives = 53/55 (96.36%), Query Frame = 1 
Query:    7 VRKETLRLRMAIPLLVPHMNLHDAKLGGYDVPAESKILVNAWWLANNPAQWKKPE 171
            V KETLRLRMAIPLLVPHMNLHDAKL G+D+PAESKILVNAWWLANNPAQWKKPE
Sbjct:  359 VIKETLRLRMAIPLLVPHMNLHDAKLSGFDIPAESKILVNAWWLANNPAQWKKPE 413

HSP 3 Score: 25.0238 bits (53), Expect = 5.128e-57
Identity = 11/18 (61.11%), Postives = 11/18 (61.11%), Query Frame = 2 
Query:  149 PPNGKNLNEFTPERFLEE 202
            P   K   EF PERFLEE
Sbjct:  406 PAQWKKPEEFRPERFLEE 423
[Close]
BLAST of EY756844 vs. ExPASy Swiss-Prot
Match: TCMO_POPKI (Trans-cinnamate 4-monooxygenase OS=Populus kitakamiensis GN=CYP73A16 PE=2 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 5.128e-57
Identity = 62/85 (72.94%), Postives = 65/85 (76.47%), Query Frame = 3 
Query:  192 FWRRESKVEANGNDFRYLPFGVERKSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKIDTAEKGGQVSLHILKHSTIVAKPRSF 446
            F    +KVEANGNDFRYLPFGV R+SCP              RLVQNFELLPPPGQSKIDT+EKGGQ SLHILKHSTIVAKPRSF
Sbjct:  421 FLEEGAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKIDTSEKGGQFSLHILKHSTIVAKPRSF 505

HSP 2 Score: 110.538 bits (275), Expect = 5.128e-57
Identity = 50/55 (90.91%), Postives = 52/55 (94.55%), Query Frame = 1 
Query:    7 VRKETLRLRMAIPLLVPHMNLHDAKLGGYDVPAESKILVNAWWLANNPAQWKKPE 171
            V KETLRLRMAIPLLVPHMNLHDAKLGG+D+PAESKILVNAWWLANNPA WK PE
Sbjct:  360 VVKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPAHWKNPE 414

HSP 3 Score: 27.7202 bits (60), Expect = 5.128e-57
Identity = 12/18 (66.67%), Postives = 13/18 (72.22%), Query Frame = 2 
Query:  149 PPNGKNLNEFTPERFLEE 202
            P + KN  EF PERFLEE
Sbjct:  407 PAHWKNPEEFRPERFLEE 424
[Close]
BLAST of EY756844 vs. ExPASy Swiss-Prot
Match: TCMO_ZINEL (Trans-cinnamate 4-monooxygenase OS=Zinnia elegans GN=CYP73A12 PE=2 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 1.915e-56
Identity = 60/83 (72.29%), Postives = 62/83 (74.70%), Query Frame = 3 
Query:  192 FWRRESKVEANGNDFRYLPFGVERKSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKIDTAEKGGQVSLHILKHSTIVAKPR 440
            F   ESKVEANGNDFRYLPFGV R+SCP              RLVQNFELLPPPGQ K+DT EKGGQ SLHILKHSTIVAKPR
Sbjct:  421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQDKVDTTEKGGQFSLHILKHSTIVAKPR 503

HSP 2 Score: 113.62 bits (283), Expect = 1.915e-56
Identity = 52/55 (94.55%), Postives = 53/55 (96.36%), Query Frame = 1 
Query:    7 VRKETLRLRMAIPLLVPHMNLHDAKLGGYDVPAESKILVNAWWLANNPAQWKKPE 171
            V KETLRLRMAIPLLVPHMNLHDAKLGGYD+PAESKILVNAWWLANNP QWKKPE
Sbjct:  360 VIKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPDQWKKPE 414

HSP 3 Score: 25.0238 bits (53), Expect = 1.915e-56
Identity = 11/18 (61.11%), Postives = 11/18 (61.11%), Query Frame = 2 
Query:  149 PPNGKNLNEFTPERFLEE 202
            P   K   EF PERFLEE
Sbjct:  407 PDQWKKPEEFRPERFLEE 424
[Close]
BLAST of EY756844 vs. ExPASy Swiss-Prot
Match: TCMO_SOYBN (Trans-cinnamate 4-monooxygenase OS=Glycine max GN=CYP73A11 PE=2 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 2.258e-56
Identity = 62/85 (72.94%), Postives = 66/85 (77.65%), Query Frame = 3 
Query:  192 FWRRESKVEANGNDFRYLPFGVERKSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKIDTAEKGGQVSLHILKHSTIVAKPRSF 446
            F+  ES VEANGNDFRYLPFGV R+SCP              RLVQNFELLPPPGQS+IDT+EKGGQ SLHILKHSTIVAKPRSF
Sbjct:  422 FFEEESLVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSQIDTSEKGGQFSLHILKHSTIVAKPRSF 506

HSP 2 Score: 114.39 bits (285), Expect = 2.258e-56
Identity = 55/67 (82.09%), Postives = 56/67 (83.58%), Query Frame = 1 
Query:    7 VRKETLRLRMAIPLLVPHMNLHDAKLGGYDVPAESKILVNAWWLANNPAQWKKPE*IHA*EVFGGES 207
            V KETLRLRMAIPLLVPHMNLHDAKLGGYD+PAESKILVNAWWLANNPA WKKPE       F  ES
Sbjct:  361 VVKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPERFFEEES 427
[Close]
BLAST of EY756844 vs. ExPASy Swiss-Prot
Match: TCMO_PHAAU (Trans-cinnamate 4-monooxygenase OS=Phaseolus aureus GN=CYP73A2 PE=1 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 2.258e-56
Identity = 61/85 (71.76%), Postives = 66/85 (77.65%), Query Frame = 3 
Query:  192 FWRRESKVEANGNDFRYLPFGVERKSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKIDTAEKGGQVSLHILKHSTIVAKPRSF 446
            F+  ES VEANGNDFRYLPFGV R+SCP              RLVQNFELLPPPGQS+IDT+EKGGQ SLHILKHST+VAKPRSF
Sbjct:  421 FFEEESHVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSQIDTSEKGGQFSLHILKHSTVVAKPRSF 505

HSP 2 Score: 114.39 bits (285), Expect = 2.258e-56
Identity = 55/67 (82.09%), Postives = 56/67 (83.58%), Query Frame = 1 
Query:    7 VRKETLRLRMAIPLLVPHMNLHDAKLGGYDVPAESKILVNAWWLANNPAQWKKPE*IHA*EVFGGES 207
            V KETLRLRMAIPLLVPHMNLHDAKLGGYD+PAESKILVNAWWLANNPA WKKPE       F  ES
Sbjct:  360 VVKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPERFFEEES 426
[Close]
BLAST of EY756844 vs. ExPASy Swiss-Prot
Match: TCMO_PEA (Trans-cinnamate 4-monooxygenase OS=Pisum sativum GN=CYP73A9 PE=2 SV=2)

HSP 1 Score: 124.02 bits (310), Expect = 5.496e-56
Identity = 61/85 (71.76%), Postives = 65/85 (76.47%), Query Frame = 3 
Query:  192 FWRRESKVEANGNDFRYLPFGVERKSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKIDTAEKGGQVSLHILKHSTIVAKPRSF 446
            F   E+ VEANGNDFRYLPFGV R+SCP              RLVQNFELLPPPGQSKIDT+EKGGQ SLHILKHSTIVAKPR+F
Sbjct:  421 FLEEEAHVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKIDTSEKGGQFSLHILKHSTIVAKPRAF 505

HSP 2 Score: 111.694 bits (278), Expect = 5.496e-56
Identity = 51/55 (92.73%), Postives = 53/55 (96.36%), Query Frame = 1 
Query:    7 VRKETLRLRMAIPLLVPHMNLHDAKLGGYDVPAESKILVNAWWLANNPAQWKKPE 171
            V KETLRLRMAIPLLVPHMNLHDAKLGG+D+PAESKILVNAWWLANNPA WKKPE
Sbjct:  360 VIKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPALWKKPE 414

HSP 3 Score: 23.8682 bits (50), Expect = 5.496e-56
Identity = 11/18 (61.11%), Postives = 11/18 (61.11%), Query Frame = 2 
Query:  149 PPNGKNLNEFTPERFLEE 202
            P   K   EF PERFLEE
Sbjct:  407 PALWKKPEEFRPERFLEE 424
[Close]
BLAST of EY756844 vs. ExPASy Swiss-Prot
Match: TCMO_CATRO (Trans-cinnamate 4-monooxygenase OS=Catharanthus roseus GN=CYP73A4 PE=2 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 1.298e-54
Identity = 61/85 (71.76%), Postives = 65/85 (76.47%), Query Frame = 3 
Query:  192 FWRRESKVEANGNDFRYLPFGVERKSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKIDTAEKGGQVSLHILKHSTIVAKPRSF 446
            F   ESKVEANGNDFRYLPFGV R+SCP              RLVQNFELLPPPG+SKIDT+EKGGQ SLHILKHSTIV KPR+F
Sbjct:  421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGKSKIDTSEKGGQFSLHILKHSTIVLKPRTF 505

HSP 2 Score: 105.531 bits (262), Expect = 1.298e-54
Identity = 48/55 (87.27%), Postives = 51/55 (92.73%), Query Frame = 1 
Query:    7 VRKETLRLRMAIPLLVPHMNLHDAKLGGYDVPAESKILVNAWWLANNPAQWKKPE 171
            V KETLRLRMAIPL +PHMNLHDAKLGGYD+PAESKILVNAW+LANNP  WKKPE
Sbjct:  360 VIKETLRLRMAIPLFLPHMNLHDAKLGGYDIPAESKILVNAWFLANNPEHWKKPE 414

HSP 3 Score: 25.409 bits (54), Expect = 1.298e-54
Identity = 11/18 (61.11%), Postives = 12/18 (66.67%), Query Frame = 2 
Query:  149 PPNGKNLNEFTPERFLEE 202
            P + K   EF PERFLEE
Sbjct:  407 PEHWKKPEEFRPERFLEE 424
The following BLAST results are available for this feature:
BLAST of EY756844 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 38
Match NameE-valueIdentityDescription
TCMO_GLYEC3.674e-5874.12Trans-cinnamate 4-monooxygenase OS=Glycyrrhiza ech... [more]
TCMO_POPTM6.225e-5874.12Trans-cinnamate 4-monooxygenase OS=Populus tremulo... [more]
TCMO_HELTU3.027e-5774.12Trans-cinnamate 4-monooxygenase OS=Helianthus tube... [more]
TCMO_CICAR5.128e-5774.12Trans-cinnamate 4-monooxygenase OS=Cicer arietinum... [more]
TCMO_POPKI5.128e-5772.94Trans-cinnamate 4-monooxygenase OS=Populus kitakam... [more]
TCMO_ZINEL1.915e-5672.29Trans-cinnamate 4-monooxygenase OS=Zinnia elegans ... [more]
TCMO_SOYBN2.258e-5672.94Trans-cinnamate 4-monooxygenase OS=Glycine max GN=... [more]
TCMO_PHAAU2.258e-5671.76Trans-cinnamate 4-monooxygenase OS=Phaseolus aureu... [more]
TCMO_PEA5.496e-5671.76Trans-cinnamate 4-monooxygenase OS=Pisum sativum G... [more]
TCMO_CATRO1.298e-5471.76Trans-cinnamate 4-monooxygenase OS=Catharanthus ro... [more]

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Properties
Property NameValue
Genbank descriptionCS13-C1-001-014-C08-CT.F Sweet orange leaf, field plant B Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY756844 ID=EY756844; Name=EY756844; organism=Citrus sinensis; type=EST; length=702bp
CCACGCGTCCGCAAGGAAACTCTTCGCCTTAGGATGGCAATTCCTCTTCT
TGTGCCGCACATGAACCTTCACGACGCCAAGCTTGGAGGCTACGACGTCC
CTGCTGAGAGCAAAATCCTAGTGAACGCGTGGTGGCTCGCCAACAATCCC
GCCCAATGGAAAAAACCTGAATGAATTCACGCCTGAGAGGTTTTTGGAGG
AGAGAGTCCAAGGTTGAAGCTAATGGAAATGACTTCCGTTACCTTCCTTT
CGGCGTTGAGAGAAAAAGCTGCCCTGGAATTATCCTTGCTTTGCCAATTC
TTGGTATCACTATTGGGCGTTTGGTACAGAACTTTGAGCTCTTGCCTCCT
CCAGGTCAATCTAAGATTGACACTGCAGAGAAAGGTGGGCAGGTCAGTTT
GCACATTTTGAAGCACTCGACCATTGTTGCCAAGCCTAGATCATTCTAGT
TTCACAATTCCATTTGGCTGTAATGTTAAGTCTGTTTCAGAGTGGTTGAA
TGGTTTCTTCAATGTCGCATCAAATCAAGTGGCAGTTGGCTAGCTATATA
ATACTTGCGTAAATTTGTGAACTTAAATCTTCAAATCTTCATTTAAGATT
TGCAATAAGTTTTATGGTTTGCTTCTTAATGCAATTACTAGAAATAAATT
AATTCCGTCCTCGCTTTTGGGATAAATTCCGGGTCATGGTTCGCATTGTT
GG
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