Genetic transformation of sweet orange with the coat protein gene of Citrus psorosis virus and evaluation of resistance against the virus

Publication Overview
TitleGenetic transformation of sweet orange with the coat protein gene of Citrus psorosis virus and evaluation of resistance against the virus
AuthorsZanek MC, Reyes CA, Cervera M, Peña EJ, Velázquez K, Costa N, Plata MI, Grau O, Peña L, García ML
TypeJournal Article
Journal NamePlant cell reports
Volume27
Issue1
Year2008
Page(s)57-66
CitationZanek MC, Reyes CA, Cervera M, Peña EJ, Velázquez K, Costa N, Plata MI, Grau O, Peña L, García ML. Genetic transformation of sweet orange with the coat protein gene of Citrus psorosis virus and evaluation of resistance against the virus. Plant cell reports. 2008; 27(1):57-66.

Abstract

Citrus psorosis is a serious viral disease affecting citrus trees in many countries. Its causal agent is Citrus psorosis virus (CPsV), the type member of genus Ophiovirus. CPsV infects most important citrus varieties, including oranges, mandarins and grapefruits, as well as hybrids and citrus relatives used as rootstocks. Certification programs have not been sufficient to control the disease and no sources of natural resistance have been found. Pathogen-derived resistance (PDR) can provide an efficient alternative to control viral diseases in their hosts. For this purpose, we have produced 21 independent lines of sweet orange expressing the coat protein gene of CPsV and five of them were challenged with the homologous CPV 4 isolate. Two different viral loads were evaluated to challenge the transgenic plants, but so far, no resistance or tolerance has been found in any line after 1 year of observations. In contrast, after inoculation all lines showed characteristic symptoms of psorosis in the greenhouse. The transgenic lines expressed low and variable amounts of the cp gene and no correlation was found between copy number and transgene expression. One line contained three copies of the cp gene, expressed low amounts of the mRNA and no coat protein. The ORF was cytosine methylated suggesting a PTGS mechanism, although the transformant failed to protect against the viral load used. Possible causes for the failed protection against the CPsV are discussed.
Features
This publication contains information about 10 features:
Feature NameUniquenameType
48Kdir48Kdirgenetic_marker
AAgenetic_marker
BBgenetic_marker
C2C2genetic_marker
CPV1CPV1genetic_marker
CPV2CPV2genetic_marker
CPV3CPV3genetic_marker
CPV4CPV4genetic_marker
GUS1GUS1genetic_marker
GUS2GUS2genetic_marker
Stocks
This publication contains information about 1 stocks:
Stock NameUniquenameType
PineapplePineappleaccession
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2008
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1007/s00299-007-0422-8
KeywordsCitrus psorosis virus, Citrus, PDR, Post-transcriptional gene silencing, Transgenic trees