Phospholipase A₂ and postharvest peel pitting in citrus fruit

Publication Overview
TitlePhospholipase A₂ and postharvest peel pitting in citrus fruit
AuthorsAlferez F, Lluch Y, Burns JK
TypeJournal Article
Journal NamePostharvest biology and technology
Volume49
Issue1
Year2008
Page(s)69-76
CitationAlferez F, Lluch Y, Burns JK. Phospholipase A₂ and postharvest peel pitting in citrus fruit. Postharvest biology and technology. 2008; 49(1):69-76.

Abstract

The role of phospholipase A₂ (PLA₂) activity in development of postharvest peel pitting in mature 'Fallglo' tangerines [Bower citrus hybrid (Citrus reticulata Blancox C. reticulata Blancox C. paradisi Macf.)xTemple (C. reticulata Blancox Citrus sinensis L.)] and 'Navel' oranges (Citrus sinensis L. Osbeck) was investigated. Changes in RH from 30% to 90% followed by fruit waxing increased electrolyte leakage and PLA₂ activity in flavedo, and induced pitting. Treatment with an aqueous dip of aristolochic acid (AT), a specific inhibitor of secretory phospholipase A₂ (sPLA₂) activity, immediately before transfer from 30% to 90% RH storage, markedly reduced peel pitting symptoms. Five genes encoding various phospholipase As isolated from citrus (three patatin-like and two sPLA₂-like sequences) differentially accumulated in healthy areas, areas with developing lesions and necrotic lesions of disordered fruit. Other PLA₂, phospholipase C, and phospholipase D inhibitors also reduced peel pitting; however, PLA₂ inhibitors were the most effective in preventing the disorder. In addition, phospholipase inhibitors promoted fruit decay, suggesting that innate resistance is impacted by phospholipase action. Together, the results provide evidence for involvement of phospholipase activity in development of postharvest peel pitting symptoms in citrus fruit.
Features
This publication contains information about 6 features:
Feature NameUniquenameType
CsGAPDHCsGAPDHgenetic_marker
CsPAT1CsPAT1genetic_marker
CsPAT2CsPAT2genetic_marker
CsPAT3CsPAT3genetic_marker
CssPLA2αCssPLA2αgenetic_marker
CssPLA2βCssPLA2βgenetic_marker
Stocks
This publication contains information about 4 stocks:
Stock NameUniquenameType
Bower Citrus HybridBower Citrus Hybridbreeding_research_material
FallgloFallglocultivar
NavelNavelcultivar
TempleTemplebreeding_research_material
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2008
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1016/j.postharvbio.2008.01.010
Keywordstangerines, oranges, postharvest diseases, citrus peels, phospholipase A2, enzyme activity, electrolytes, relative humidity, wax coatings, enzyme inhibitors, gene expression, nucleotide sequences, necrosis, postharvest physiology, peel pitting, aristolochic acid, molecular sequence data
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4040948