Production and molecular characterization of potential seedless cybrid plants between pollen sterile Satsuma mandarin and two seedy Citrus cultivars

Publication Overview
TitleProduction and molecular characterization of potential seedless cybrid plants between pollen sterile Satsuma mandarin and two seedy Citrus cultivars
AuthorsCai X, Fu J, Deng X, Guo W
TypeJournal Article
Journal NamePlant cell, tissue, and organ culture
Volume90
Issue3
Year2007
Page(s)275-283
CitationCai X, Fu J, Deng X, Guo W. Production and molecular characterization of potential seedless cybrid plants between pollen sterile Satsuma mandarin and two seedy Citrus cultivars. Plant cell, tissue, and organ culture. 2007; 90(3):275-283.

Abstract

Cytoplasmic male sterility (CMS) is known to be controlled by mitochondrial genome in higher plants including Satsuma mandarin (Citrus unshiu Marc.). Citrus symmetric fusion experiments often produce diploid cybrids possessing nuclear DNA from the mesophyll parent and mitochondrial DNA (mtDNA) from the embryogenic callus parent. Therefore, it is possible to transfer CMS from Satsuma mandarin as callus parent to seedy citrus cultivars as leaf one by somatic cybridization. Herein, symmetric fusion technique was adopted to create cybrids for potential seedlessness by transferring CMS from Citrus unshiu Marc. cv. Guoqing No. 1 (G1) to two traditional Chinese seedy citrus cultivars, 'Shatian' pummelo (C. grandis (L) Osbeck) and 'Bingtang' orange (C. sinensis (L) Osbeck). Flow cytometry analysis showed that 19 plants recovered from G1 + 'Bingtang' orange and 17 of 35 plants regenerated from G1 + 'Shatian' pummelo were diploid. The remaining plants from G1 + 'Shatian' pummelo were tetraploid. The diploid plants from the two combinations were confirmed as true cybrids by simple sequence repeat (SSR) and cleaved amplified polymorphic sequence (CAPS) analysis, with nuclear DNA from their corresponding leaf parent and mtDNA from their common suspension parent, G1 Satsuma mandarin. The remaining plants from G1 + 'Shatian' pummelo were identified as somatic hybrids with mtDNA from G1. The chloroplast simple sequence repeat (cp-SSR) analysis revealed somatic hybrid/cybrid plants from the two combinations in most cases possessed either of their parental chloroplast type, and two plants from G1 +'Shatian' pummelo and all embryoids analyzed from G1 + 'Bingtang' orange possessed chloroplast DNA (cpDNA) from both parents. These results demonstrated that we succeeded in introducing mtDNA from G1 Satsuma mandarin into the two target seedy citrus cultivars for potential seedlessness through symmetric fusion.
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
Bingtang orangeBingtang orangecultivar
Satsuma mandarinSatsuma mandarincultivar
Shatian pummeloShatian pummelocultivar
Features
This publication contains information about 2 features:
Feature NameUniquenameType
NTCP9NTCP9genetic_marker
TAA1TAA1genetic_marker
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2007
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1007/s11240-007-9266-8
KeywordsCitrus unshiu, mandarins, Citrus sinensis, oranges, plant breeding, seedless varieties, cybrids, genetic variation, pollen, cytoplasmic male sterility, gene transfer, mitochondrial DNA, gene fusion
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:3939849