Mature Juice Sac from Navel Orange in Vulnus5 vector

Overview
Library NameMature Juice Sac from Navel Orange in Vulnus5 vector
Unique NameMature Juice Sac from Navel Orange in Vulnus5 vector
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: juice sac; Vector: Vulnus5; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Juice sac tissue was separated from the rest of the fruit and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 3 84 well dishes.
Features
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Feature NameUnique NameType
DN135279DN135279EST
DN135278DN135278EST
DN135277DN135277EST
DN135276DN135276EST
DN135275DN135275EST
DN135274DN135274EST
DN135273DN135273EST
DN135272DN135272EST
DN135271DN135271EST
DN135270DN135270EST
DN135269DN135269EST
DN135268DN135268EST
DN135267DN135267EST
DN135266DN135266EST
DN135265DN135265EST
DN135264DN135264EST
DN135263DN135263EST
DN135262DN135262EST
DN135261DN135261EST
DN135260DN135260EST
DN135259DN135259EST
DN135258DN135258EST
DN135257DN135257EST
DN135256DN135256EST
DN135255DN135255EST

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Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageMature fruit sample - collected January 2002
Genbank library noteOrgan: juice sac; Vector: Vulnus5; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Juice sac tissue was separated from the rest of the fruit and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 3 84 well dishes.
Fruittissue type