Mature Juice Sac from Navel Orange in Vulnus5 vector

Overview
Library NameMature Juice Sac from Navel Orange in Vulnus5 vector
Unique NameMature Juice Sac from Navel Orange in Vulnus5 vector
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: juice sac; Vector: Vulnus5; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Juice sac tissue was separated from the rest of the fruit and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 3 84 well dishes.
Features
The following browser provides a quick view for new visitors. Use the searching mechanism to find specific features.
Feature NameUnique NameType
DN135216DN135216EST
DN135217DN135217EST
DN135218DN135218EST
DN135220DN135220EST
DN135221DN135221EST
DN135222DN135222EST
DN135223DN135223EST
DN135224DN135224EST
DN135225DN135225EST
DN135226DN135226EST
DN135227DN135227EST
DN135228DN135228EST
DN135229DN135229EST
DN135230DN135230EST
DN135231DN135231EST
DN135232DN135232EST
DN135233DN135233EST
DN135234DN135234EST
DN135235DN135235EST
DN135236DN135236EST
DN135237DN135237EST
DN135238DN135238EST
DN135239DN135239EST
DN135240DN135240EST
DN135241DN135241EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageMature fruit sample - collected January 2002
Genbank library noteOrgan: juice sac; Vector: Vulnus5; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Juice sac tissue was separated from the rest of the fruit and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 3 84 well dishes.
Fruittissue type