Mature Juice Sac from Navel Orange in pTriplEx2 vector

Overview
Library NameMature Juice Sac from Navel Orange in pTriplEx2 vector
Unique NameMature Juice Sac from Navel Orange in pTriplEx2 vector
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: juice sac; Vector: pTriplEx2; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Juice sac tissue was separated from the rest of the fruit and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 3 84 well dishes.
Features
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Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageMature fruit sample - collected January 2002
Genbank library noteOrgan: juice sac; Vector: pTriplEx2; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Juice sac tissue was separated from the rest of the fruit and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 3 84 well dishes.
Fruittissue type