Developing fruit flavedo at 165 DAFB

Overview
Library NameDeveloping fruit flavedo at 165 DAFB
Unique NameDeveloping fruit flavedo at 165 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on October 22, 2003, between 8 to 9 am and stored at 4C. The flavedo tissue was dissected out of developing fruit (165 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
Array NameDeveloping fruit flavedo at 165 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
The following browser provides a quick view for new visitors. Use the searching mechanism to find specific features.
Feature NameUnique NameType
CK939273CK939273EST
CK939274CK939274EST
CK939275CK939275EST
CK939276CK939276EST
CK939277CK939277EST
CK939278CK939278EST
CK939279CK939279EST
CK939280CK939280EST
CK939281CK939281EST
CK939282CK939282EST
CK939283CK939283EST
CK939284CK939284EST
CK939285CK939285EST
CK939286CK939286EST
CK939287CK939287EST
CK939288CK939288EST
CK939289CK939289EST
CK939290CK939290EST
CK939291CK939291EST
CK939292CK939292EST
CK939293CK939293EST
CK939294CK939294EST
CK939295CK939295EST
CK939296CK939296EST
CK939297CK939297EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageDeveloping fruit sample-collected October 22, 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on October 22, 2003, between 8 to 9 am and stored at 4C. The flavedo tissue was dissected out of developing fruit (165 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type