Developing fruit albedo at 80 DAFB in Vulnus5 vector

Overview
Library NameDeveloping fruit albedo at 80 DAFB in Vulnus5 vector
Unique NameDeveloping fruit albedo at 80 DAFB in Vulnus5 vector
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: Vulnus5; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on July 29, 2003, between 8 to 9 am and stored at 4C. The albedo tissue was dissected out of developing fruit (80 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Features
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Feature NameUnique NameType
DN134763DN134763EST
DN134764DN134764EST
DN134765DN134765EST
DN134766DN134766EST
DN134767DN134767EST
DN134768DN134768EST
DN134769DN134769EST
DN134770DN134770EST
DN134771DN134771EST
DN134772DN134772EST
DN134773DN134773EST
DN134774DN134774EST
DN134775DN134775EST
DN134776DN134776EST
DN134777DN134777EST
DN134778DN134778EST
DN134779DN134779EST
DN134780DN134780EST
DN134781DN134781EST
DN134782DN134782EST
DN134783DN134783EST
DN134784DN134784EST
DN134785DN134785EST
DN134786DN134786EST
DN134787DN134787EST

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Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageDeveloping fruit albedo-collected July 29, 2003
Genbank library noteOrgan: Fruit; Vector: Vulnus5; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on July 29, 2003, between 8 to 9 am and stored at 4C. The albedo tissue was dissected out of developing fruit (80 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type