Citrus clementina pistils cDNA-AFLP library

Overview
Library NameCitrus clementina pistils cDNA-AFLP library
Unique NameCitrus clementina pistils cDNA-AFLP library
OrganismCitrus clementina (Clementine)
Typecdna_library
Vector: pGEM-T easy; Site_1: EcoRI; Site_2: MseI; Total RNA was extracted using Plant RNA Reagent (Invitrogen) from unpollinated and self-pollinated pistils of Comune clementine and its self-compatible natural mutation Monreal, grown at the experimental station of Catania University. About 100 ug total RNA was used to isolate mRNA by PolyATract mRNA Isolation System III (Promega). cDNA synthesis and cDNA-AFLP analysis were carried according to Bachem et al (1996). Differentially expressed and polymorphic sequences were visually screened and excised from gels, eluted overnight with water, reamplified with the same conditions used for selective amplifications and electrophoresed in 1% agarose gel. All the successfully reamplified TDFs were excised from agarose, purified with QIAquick gel extraction kit (Qiagen), cloned into pgem-T Easy vector (Promega), and sequenced using an ABI310 genetic analyzer (Applied Biosystems). Seqman software (DNAstar, Madison,WI, USA) was used to remove vector sequences and to find possible overlaps among the transcript derived fragments.
Features
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Feature NameUnique NameType
GH733328GH733328EST
GH733327GH733327EST
GH733326GH733326EST
GH733325GH733325EST
GH733322GH733322EST
GH733321GH733321EST
GH733320GH733320EST
GH733319GH733319EST
GH733318GH733318EST
GH733317GH733317EST
GH733316GH733316EST
GH733315GH733315EST
GH733314GH733314EST
GH733313GH733313EST
GH733312GH733312EST
GH733311GH733311EST
GH733310GH733310EST
GH733309GH733309EST
GH733308GH733308EST
GH733307GH733307EST
GH733306GH733306EST
GH733305GH733305EST
GH733304GH733304EST
GH733303GH733303EST
GH733302GH733302EST

Pages

Properties
Property NameValue
Genbank library cultivarComune and Monreal
Genbank library noteVector: pGEM-T easy; Site_1: EcoRI; Site_2: MseI; Total RNA was extracted using Plant RNA Reagent (Invitrogen) from unpollinated and self-pollinated pistils of Comune clementine and its self-compatible natural mutation Monreal, grown at the experimental station of Catania University. About 100 ug total RNA was used to isolate mRNA by PolyATract mRNA Isolation System III (Promega). cDNA synthesis and cDNA-AFLP analysis were carried according to Bachem et al (1996). Differentially expressed and polymorphic sequences were visually screened and excised from gels, eluted overnight with water, reamplified with the same conditions used for selective amplifications and electrophoresed in 1% agarose gel. All the successfully reamplified TDFs were excised from agarose, purified with QIAquick gel extraction kit (Qiagen), cloned into pgem-T Easy vector (Promega), and sequenced using an ABI310 genetic analyzer (Applied Biosystems). Seqman software (DNAstar, Madison,WI, USA) was used to remove vector sequences and to find possible overlaps among the transcript derived fragments.
Genbank library tissue typeUnpollinated pistils or self-pollinated pistils
Gynoeciumtissue type