Citrus clementina pistils cDNA-AFLP library

Overview
Library NameCitrus clementina pistils cDNA-AFLP library
Unique NameCitrus clementina pistils cDNA-AFLP library
OrganismCitrus clementina (Clementine)
Typecdna_library
Vector: pGEM-T easy; Site_1: EcoRI; Site_2: MseI; Total RNA was extracted using Plant RNA Reagent (Invitrogen) from unpollinated and self-pollinated pistils of Comune clementine and its self-compatible natural mutation Monreal, grown at the experimental station of Catania University. About 100 ug total RNA was used to isolate mRNA by PolyATract mRNA Isolation System III (Promega). cDNA synthesis and cDNA-AFLP analysis were carried according to Bachem et al (1996). Differentially expressed and polymorphic sequences were visually screened and excised from gels, eluted overnight with water, reamplified with the same conditions used for selective amplifications and electrophoresed in 1% agarose gel. All the successfully reamplified TDFs were excised from agarose, purified with QIAquick gel extraction kit (Qiagen), cloned into pgem-T Easy vector (Promega), and sequenced using an ABI310 genetic analyzer (Applied Biosystems). Seqman software (DNAstar, Madison,WI, USA) was used to remove vector sequences and to find possible overlaps among the transcript derived fragments.
Features
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Feature NameUnique NameType
GH733233GH733233EST
GH733235GH733235EST
GH733236GH733236EST
GH733237GH733237EST
GH733238GH733238EST
GH733239GH733239EST
GH733240GH733240EST
GH733241GH733241EST
GH733242GH733242EST
GH733243GH733243EST
GH733244GH733244EST
GH733245GH733245EST
GH733246GH733246EST
GH733247GH733247EST
GH733248GH733248EST
GH733249GH733249EST
GH733250GH733250EST
GH733251GH733251EST
GH733252GH733252EST
GH733254GH733254EST
GH733255GH733255EST
GH733257GH733257EST
GH733259GH733259EST
GH733260GH733260EST
GH733261GH733261EST

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Properties
Property NameValue
Genbank library cultivarComune and Monreal
Genbank library noteVector: pGEM-T easy; Site_1: EcoRI; Site_2: MseI; Total RNA was extracted using Plant RNA Reagent (Invitrogen) from unpollinated and self-pollinated pistils of Comune clementine and its self-compatible natural mutation Monreal, grown at the experimental station of Catania University. About 100 ug total RNA was used to isolate mRNA by PolyATract mRNA Isolation System III (Promega). cDNA synthesis and cDNA-AFLP analysis were carried according to Bachem et al (1996). Differentially expressed and polymorphic sequences were visually screened and excised from gels, eluted overnight with water, reamplified with the same conditions used for selective amplifications and electrophoresed in 1% agarose gel. All the successfully reamplified TDFs were excised from agarose, purified with QIAquick gel extraction kit (Qiagen), cloned into pgem-T Easy vector (Promega), and sequenced using an ABI310 genetic analyzer (Applied Biosystems). Seqman software (DNAstar, Madison,WI, USA) was used to remove vector sequences and to find possible overlaps among the transcript derived fragments.
Genbank library tissue typeUnpollinated pistils or self-pollinated pistils
Gynoeciumtissue type