Expressed sequence enrichment for candidate gene analysis of citrus tristeza virus resistance

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TitleExpressed sequence enrichment for candidate gene analysis of citrus tristeza virus resistance
AuthorsBernet G, Breto M, Asins M
TypeJournal Article
Journal NameTheoretical and applied genetics
CitationBernet G, Breto M, Asins M. Expressed sequence enrichment for candidate gene analysis of citrus tristeza virus resistance. Theoretical and applied genetics. 2004; 108(4):592-602.


Several studies have reported markers linked to a putative resistance gene from Poncirus trifoliata (Ctv-R) located at linkage group 4 that confers resistance against one of the most important citrus pathogens, citrus tristeza virus (CTV). To be successful in both marker-assisted selection and transformation experiments, its accurate mapping is needed. Several factors may affect its localization, among them two are considered here: the definition of resistance and the genetic background of progeny. Two progenies derived from P. trifoliata, by self-pollination and by crossing with sour orange (Citrus aurantium), a citrus rootstock well-adapted to arid and semi-arid areas, were used for linkage group-4 marker enrichment. Two new methodologies were used to enrich this region with expressed sequences. The enrichment of group 4 resulted in the fusion of several C. aurantium linkage groups. The new one A(7+3+4) is now saturated with 48 markers including expressed sequences. Surprisingly, sour orange was as resistant to the CTV isolate tested as was P. trifoliata, and three hybrids that carry Ctv-R, as deduced from its flanking markers, are susceptible to CTV. The new linkage maps were used to map Ctv-R under the hypothesis of monogenic inheritance. Its position on linkage group 4 of P. trifoliata differs from the location previously reported in other progenies. The genetic analysis of virus-plant interaction in the family derived from C. aurantium after a CTV chronic infection showed the segregation of five types of interaction, which is not compatible with the hypothesis of a single gene controlling resistance. Two major issues are discussed: another type of genetic analysis of CTV resistance is needed to avoid the assumption of monogenic inheritance, and transferring Ctv-R from P. trifoliata to sour orange might not avoid the CTV decline of sweet orange trees.
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Stock NameUniquenameType
Afin vernaAfin vernacultivar
flying dragonflying dragoncultivar
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Publication TypeJournal Article
Publication Date2004
Published Location|||
Language Abbreng
KeywordsPoncirus trifoliata, Citrus aurantium, sour oranges, hybrids, intergeneric hybridization, rootstocks, genes, genetic resistance, disease resistance, Citrus tristeza virus, plant viruses, chromosome mapping, genetic markers, genetic techniques and protocols, linkage groups, differential display analysis, bulk segregant analysis, cDNA subtraction library, Ctv-R gene
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This publication is also available in the following databases:
AGL: USDA National Agricultural LibraryAGL:3624411
PMID: PubMedPMID:14624336