CX295024

Overview
NameCX295024
Unique NameCX295024
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length735
Libraries
Library NameType
DroRLeaf1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig9488 contig Ccv1_Contig9488:153..887. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CX295024 vs. ExPASy Swiss-Prot
Match: AL2B4_ARATH (Aldehyde dehydrogenase family 2 member B4, mitochondrial OS=Arabidopsis thaliana GN=ALDH2B4 PE=1 SV=1)

HSP 1 Score: 415.231 bits (1066), Expect = 1.781e-115
Identity = 192/243 (79.01%), Postives = 230/243 (94.65%), Query Frame = 2 
Query:    2 AGLPPGVLNVVSGYGPTAGAALASHMDVDKLSFTGHCDTGKIVQELAAKSNLKPVTLELGGKSPFIIFDDADVDQAVELAHFALFYNQGQCCCAGSRTYVHERVYDEFVEKAKARAMRRIVGDPFKSGVEQGPQIDPEQFEKVLRYIRSGIESNATLECGGDRLGNRGYFVQPTVFSDVKDDMLIAQDEIFGPVQSILKFKETDDVIRRANKTRFGLAAGVFTKSADTANTMMRALRAGTVWI 730
            AGLPPGVLN+VSG+G TAGAALASHMDVDKL+FTG  DTGK++  LAA SNLKPVTLELGGKSPFI+F+DAD+D+AVELAHFALF+NQGQCCCAGSRT+VHE+VYDEFVEK+KARA++R+VGDPF+ G+EQGPQID +QFEKV++YI+SGIESNATLECGGD++G++GYF+QPTVFS+VKDDMLIAQDEIFGPVQSILKF + D+VI+RAN+T++GLAAGVFTK+ DTAN + RAL+AGTVW+
Sbjct:  248 AGLPPGVLNIVSGFGATAGAALASHMDVDKLAFTGSTDTGKVILGLAANSNLKPVTLELGGKSPFIVFEDADIDKAVELAHFALFFNQGQCCCAGSRTFVHEKVYDEFVEKSKARALKRVVGDPFRKGIEQGPQIDLKQFEKVMKYIKSGIESNATLECGGDQIGDKGYFIQPTVFSNVKDDMLIAQDEIFGPVQSILKFSDVDEVIKRANETKYGLAAGVFTKNLDTANRVSRALKAGTVWV 490
[Close]
BLAST of CX295024 vs. ExPASy Swiss-Prot
Match: AL2B7_ARATH (Aldehyde dehydrogenase family 2 member B7, mitochondrial OS=Arabidopsis thaliana GN=ALDH2B7 PE=2 SV=2)

HSP 1 Score: 397.897 bits (1021), Expect = 2.943e-110
Identity = 187/243 (76.95%), Postives = 222/243 (91.36%), Query Frame = 2 
Query:    2 AGLPPGVLNVVSGYGPTAGAALASHMDVDKLSFTGHCDTGKIVQELAAKSNLKPVTLELGGKSPFIIFDDADVDQAVELAHFALFYNQGQCCCAGSRTYVHERVYDEFVEKAKARAMRRIVGDPFKSGVEQGPQIDPEQFEKVLRYIRSGIESNATLECGGDRLGNRGYFVQPTVFSDVKDDMLIAQDEIFGPVQSILKFKETDDVIRRANKTRFGLAAGVFTKSADTANTMMRALRAGTVWI 730
            AGLP GV+N+VSG+G TAGAA+ASHMDVDK++FTG  D GKI+ ELA+KSNLK VTLELGGKSPFI+ +DADVDQAVELAHFALF+NQGQCCCAGSRT+VHERVYDEFVEKAKARA++R VGDPFKSG+EQGPQ+D EQF K+L+YI+ G+E+ ATL+ GGDRLG++GY++QPTVFSDVKDDMLIA DEIFGPVQ+ILKFK+ D+VI RAN +R+GLAAGVFT++ DTA+ +MRALR GTVWI
Sbjct:  244 AGLPDGVVNIVSGFGATAGAAIASHMDVDKVAFTGSTDVGKIILELASKSNLKAVTLELGGKSPFIVCEDADVDQAVELAHFALFFNQGQCCCAGSRTFVHERVYDEFVEKAKARALKRNVGDPFKSGIEQGPQVDSEQFNKILKYIKHGVEAGATLQAGGDRLGSKGYYIQPTVFSDVKDDMLIATDEIFGPVQTILKFKDLDEVIARANNSRYGLAAGVFTQNLDTAHRLMRALRVGTVWI 486
[Close]
BLAST of CX295024 vs. ExPASy Swiss-Prot
Match: ALDH2_RAT (Aldehyde dehydrogenase, mitochondrial OS=Rattus norvegicus GN=Aldh2 PE=1 SV=1)

HSP 1 Score: 327.405 bits (838), Expect = 4.885e-89
Identity = 157/243 (64.61%), Postives = 191/243 (78.60%), Query Frame = 2 
Query:    2 AGLPPGVLNVVSGYGPTAGAALASHMDVDKLSFTGHCDTGKIVQELAAKSNLKPVTLELGGKSPFIIFDDADVDQAVELAHFALFYNQGQCCCAGSRTYVHERVYDEFVEKAKARAMRRIVGDPFKSGVEQGPQIDPEQFEKVLRYIRSGIESNATLECGGDRLGNRGYFVQPTVFSDVKDDMLIAQDEIFGPVQSILKFKETDDVIRRANKTRFGLAAGVFTKSADTANTMMRALRAGTVWI 730
            AG PPGV+N+V G+GPTAGAA+ASH DVDK++FTG  + G ++Q  A  SNLK VTLELGGKSP II  DAD+D AVE AHFALF+NQGQCCCAGSRT+V E VYDEFVE++ ARA  R+VG+PF S  EQGPQ+D  QF+K+L YI+SG +  A L CGG    +RGYF+QPTVF DVKD M IA++EIFGPV  ILKFK  ++V+ RAN +++GLAA VFTK  D AN + +AL+AGTVWI
Sbjct:  230 AGFPPGVVNIVPGFGPTAGAAIASHEDVDKVAFTGSTEVGHLIQVAAGSSNLKRVTLELGGKSPNIIMSDADMDWAVEQAHFALFFNQGQCCCAGSRTFVQEDVYDEFVERSVARAKSRVVGNPFDSRTEQGPQVDETQFKKILGYIKSGQQEGAKLLCGGGAAADRGYFIQPTVFGDVKDGMTIAKEEIFGPVMQILKFKTIEEVVGRANNSKYGLAAAVFTKDLDKANYLSQALQAGTVWI 472
[Close]
BLAST of CX295024 vs. ExPASy Swiss-Prot
Match: ALDH2_PIG (Aldehyde dehydrogenase, mitochondrial OS=Sus scrofa GN=ALDH2 PE=2 SV=1)

HSP 1 Score: 327.405 bits (838), Expect = 4.885e-89
Identity = 158/243 (65.02%), Postives = 191/243 (78.60%), Query Frame = 2 
Query:    2 AGLPPGVLNVVSGYGPTAGAALASHMDVDKLSFTGHCDTGKIVQELAAKSNLKPVTLELGGKSPFIIFDDADVDQAVELAHFALFYNQGQCCCAGSRTYVHERVYDEFVEKAKARAMRRIVGDPFKSGVEQGPQIDPEQFEKVLRYIRSGIESNATLECGGDRLGNRGYFVQPTVFSDVKDDMLIAQDEIFGPVQSILKFKETDDVIRRANKTRFGLAAGVFTKSADTANTMMRALRAGTVWI 730
            AG PPGV+N+V GYGPTAGAA+ASH DVDK++FTG  + G ++Q  A KSNLK VTLELGGKSP II  DAD+D AVE AHFALF+NQGQCCCAGSRT+V E +Y EFVE++ ARA  R+VG+PF S  EQGPQID  QF+K+L YI+SG E  A L CGG    +RGYF+QPTVF DV+D M IA++EIFGPV  ILKFK  ++VI RAN +++GLAA VFTK  D AN + +AL+AGTVW+
Sbjct:  232 AGFPPGVVNIVPGYGPTAGAAIASHEDVDKVAFTGSTEVGHLIQVAAGKSNLKRVTLELGGKSPNIIMSDADMDWAVEQAHFALFFNQGQCCCAGSRTFVQEDIYAEFVERSVARARSRVVGNPFDSRTEQGPQIDETQFKKILGYIKSGKEEGAKLLCGGGAAADRGYFIQPTVFGDVQDGMTIAKEEIFGPVMQILKFKTIEEVIGRANNSKYGLAAAVFTKDLDKANYLSQALQAGTVWV 474
[Close]
BLAST of CX295024 vs. ExPASy Swiss-Prot
Match: ALDH2_MESAU (Aldehyde dehydrogenase, mitochondrial OS=Mesocricetus auratus GN=ALDH2 PE=1 SV=1)

HSP 1 Score: 327.405 bits (838), Expect = 4.885e-89
Identity = 157/243 (64.61%), Postives = 191/243 (78.60%), Query Frame = 2 
Query:    2 AGLPPGVLNVVSGYGPTAGAALASHMDVDKLSFTGHCDTGKIVQELAAKSNLKPVTLELGGKSPFIIFDDADVDQAVELAHFALFYNQGQCCCAGSRTYVHERVYDEFVEKAKARAMRRIVGDPFKSGVEQGPQIDPEQFEKVLRYIRSGIESNATLECGGDRLGNRGYFVQPTVFSDVKDDMLIAQDEIFGPVQSILKFKETDDVIRRANKTRFGLAAGVFTKSADTANTMMRALRAGTVWI 730
            AG PPGV+N+V G+GPTAGAA+ASH DVDK++FTG  + G ++Q  A  SNLK VTLELGGKSP II  DAD+D AVE AHFALF+NQGQCCCAGSRT+V E VYDEFVE++ ARA  R+VG+PF S  EQGPQ+D  QF+K+L YI+SG +  A L CGG    +RGYF+QPTVF DVKD M IA++EIFGPV  ILKFK  ++V+ RAN +++GLAA VFTK  D AN + +AL+AGTVWI
Sbjct:  211 AGFPPGVVNIVPGFGPTAGAAIASHEDVDKVAFTGSTEVGHLIQVAAGSSNLKRVTLELGGKSPNIIMSDADMDWAVEQAHFALFFNQGQCCCAGSRTFVQEDVYDEFVERSVARAKSRVVGNPFDSRTEQGPQVDETQFKKILGYIKSGQQEGAKLLCGGGAAADRGYFIQPTVFGDVKDGMTIAKEEIFGPVMQILKFKTIEEVVGRANNSKYGLAAAVFTKDLDKANYLSQALQAGTVWI 453
[Close]
BLAST of CX295024 vs. ExPASy Swiss-Prot
Match: ALDH2_BOVIN (Aldehyde dehydrogenase, mitochondrial OS=Bos taurus GN=ALDH2 PE=1 SV=2)

HSP 1 Score: 325.865 bits (834), Expect = 1.421e-88
Identity = 156/243 (64.20%), Postives = 191/243 (78.60%), Query Frame = 2 
Query:    2 AGLPPGVLNVVSGYGPTAGAALASHMDVDKLSFTGHCDTGKIVQELAAKSNLKPVTLELGGKSPFIIFDDADVDQAVELAHFALFYNQGQCCCAGSRTYVHERVYDEFVEKAKARAMRRIVGDPFKSGVEQGPQIDPEQFEKVLRYIRSGIESNATLECGGDRLGNRGYFVQPTVFSDVKDDMLIAQDEIFGPVQSILKFKETDDVIRRANKTRFGLAAGVFTKSADTANTMMRALRAGTVWI 730
            AG PPGV+NV+ G+GPTAGAA+ASH DVDK++FTG  + G ++Q  A KSNLK VTLELGGKSP II  DAD+D AVE AHFALF+NQGQCCCAGSRT+V E +Y EFVE++ ARA  R+VG+PF S  EQGPQ+D  QF+KVL YI+SG E  A L CGG    +RGYF+QPTVF DV+D M IA++EIFGPV  ILKFK  ++V+ RAN +++GLAA VFTK  D AN + +AL+AGTVW+
Sbjct:  231 AGFPPGVVNVIPGFGPTAGAAIASHEDVDKVAFTGSTEVGHLIQVAAGKSNLKRVTLELGGKSPNIIMSDADMDWAVEQAHFALFFNQGQCCCAGSRTFVQEDIYAEFVERSVARAKSRVVGNPFDSRTEQGPQVDETQFKKVLGYIKSGKEEGAKLLCGGGAAADRGYFIQPTVFGDVQDGMTIAKEEIFGPVMQILKFKSMEEVVGRANNSKYGLAAAVFTKDLDKANYLSQALQAGTVWV 473
[Close]
BLAST of CX295024 vs. ExPASy Swiss-Prot
Match: ALDH2_MOUSE (Aldehyde dehydrogenase, mitochondrial OS=Mus musculus GN=Aldh2 PE=1 SV=1)

HSP 1 Score: 325.094 bits (832), Expect = 2.425e-88
Identity = 156/243 (64.20%), Postives = 190/243 (78.19%), Query Frame = 2 
Query:    2 AGLPPGVLNVVSGYGPTAGAALASHMDVDKLSFTGHCDTGKIVQELAAKSNLKPVTLELGGKSPFIIFDDADVDQAVELAHFALFYNQGQCCCAGSRTYVHERVYDEFVEKAKARAMRRIVGDPFKSGVEQGPQIDPEQFEKVLRYIRSGIESNATLECGGDRLGNRGYFVQPTVFSDVKDDMLIAQDEIFGPVQSILKFKETDDVIRRANKTRFGLAAGVFTKSADTANTMMRALRAGTVWI 730
            AG PPGV+N+V G+GPTAGAA+ASH  VDK++FTG  + G ++Q  A  SNLK VTLELGGKSP II  DAD+D AVE AHFALF+NQGQCCCAGSRT+V E VYDEFVE++ ARA  R+VG+PF S  EQGPQ+D  QF+K+L YI+SG +  A L CGG    +RGYF+QPTVF DVKD M IA++EIFGPV  ILKFK  ++V+ RAN +++GLAA VFTK  D AN + +AL+AGTVWI
Sbjct:  230 AGFPPGVVNIVPGFGPTAGAAIASHEGVDKVAFTGSTEVGHLIQVAAGSSNLKRVTLELGGKSPNIIMSDADMDWAVEQAHFALFFNQGQCCCAGSRTFVQENVYDEFVERSVARAKSRVVGNPFDSRTEQGPQVDETQFKKILGYIKSGQQEGAKLLCGGGAAADRGYFIQPTVFGDVKDGMTIAKEEIFGPVMQILKFKTIEEVVGRANDSKYGLAAAVFTKDLDKANYLSQALQAGTVWI 472
[Close]
BLAST of CX295024 vs. ExPASy Swiss-Prot
Match: ALDH2_PONAB (Aldehyde dehydrogenase, mitochondrial OS=Pongo abelii GN=ALDH2 PE=2 SV=1)

HSP 1 Score: 322.398 bits (825), Expect = 1.572e-87
Identity = 153/243 (62.96%), Postives = 190/243 (78.19%), Query Frame = 2 
Query:    2 AGLPPGVLNVVSGYGPTAGAALASHMDVDKLSFTGHCDTGKIVQELAAKSNLKPVTLELGGKSPFIIFDDADVDQAVELAHFALFYNQGQCCCAGSRTYVHERVYDEFVEKAKARAMRRIVGDPFKSGVEQGPQIDPEQFEKVLRYIRSGIESNATLECGGDRLGNRGYFVQPTVFSDVKDDMLIAQDEIFGPVQSILKFKETDDVIRRANKTRFGLAAGVFTKSADTANTMMRALRAGTVWI 730
            AG PPGV+N+V G+GPTAGAA+ASH DVDK++FTG  + G+++Q  A  SNLK VTLELGGKSP II  DAD+D AVE AHFALF+NQGQCCCAGSRT+V E +YDEFVE++ ARA  R+VG+PF S  EQGPQ+D  QF+K+L YI +G +  A L CGG    +RGYF+QPTVF DV+D M IA++EIFGPV  ILKFK  ++V+ RAN + +GLAA VFTK  D AN + +AL+AGTVW+
Sbjct:  228 AGFPPGVVNIVPGFGPTAGAAIASHEDVDKVAFTGSTEIGRVIQVAAGSSNLKRVTLELGGKSPNIIMSDADMDWAVEQAHFALFFNQGQCCCAGSRTFVQEDIYDEFVERSVARAKSRVVGNPFDSKTEQGPQVDETQFKKILGYINTGKQEGAKLLCGGGIAADRGYFIQPTVFGDVQDGMTIAKEEIFGPVMQILKFKTIEEVVGRANNSTYGLAAAVFTKDLDKANYLSQALQAGTVWV 470
[Close]
BLAST of CX295024 vs. ExPASy Swiss-Prot
Match: ALDH2_HUMAN (Aldehyde dehydrogenase, mitochondrial OS=Homo sapiens GN=ALDH2 PE=1 SV=2)

HSP 1 Score: 322.398 bits (825), Expect = 1.572e-87
Identity = 153/243 (62.96%), Postives = 190/243 (78.19%), Query Frame = 2 
Query:    2 AGLPPGVLNVVSGYGPTAGAALASHMDVDKLSFTGHCDTGKIVQELAAKSNLKPVTLELGGKSPFIIFDDADVDQAVELAHFALFYNQGQCCCAGSRTYVHERVYDEFVEKAKARAMRRIVGDPFKSGVEQGPQIDPEQFEKVLRYIRSGIESNATLECGGDRLGNRGYFVQPTVFSDVKDDMLIAQDEIFGPVQSILKFKETDDVIRRANKTRFGLAAGVFTKSADTANTMMRALRAGTVWI 730
            AG PPGV+N+V G+GPTAGAA+ASH DVDK++FTG  + G+++Q  A  SNLK VTLELGGKSP II  DAD+D AVE AHFALF+NQGQCCCAGSRT+V E +YDEFVE++ ARA  R+VG+PF S  EQGPQ+D  QF+K+L YI +G +  A L CGG    +RGYF+QPTVF DV+D M IA++EIFGPV  ILKFK  ++V+ RAN + +GLAA VFTK  D AN + +AL+AGTVW+
Sbjct:  228 AGFPPGVVNIVPGFGPTAGAAIASHEDVDKVAFTGSTEIGRVIQVAAGSSNLKRVTLELGGKSPNIIMSDADMDWAVEQAHFALFFNQGQCCCAGSRTFVQEDIYDEFVERSVARAKSRVVGNPFDSKTEQGPQVDETQFKKILGYINTGKQEGAKLLCGGGIAADRGYFIQPTVFGDVQDGMTIAKEEIFGPVMQILKFKTIEEVVGRANNSTYGLAAAVFTKDLDKANYLSQALQAGTVWV 470
[Close]
BLAST of CX295024 vs. ExPASy Swiss-Prot
Match: AL1A1_RAT (Retinal dehydrogenase 1 OS=Rattus norvegicus GN=Aldh1a1 PE=1 SV=3)

HSP 1 Score: 322.398 bits (825), Expect = 1.572e-87
Identity = 154/243 (63.37%), Postives = 189/243 (77.78%), Query Frame = 2 
Query:    2 AGLPPGVLNVVSGYGPTAGAALASHMDVDKLSFTGHCDTGKIVQELAAKSNLKPVTLELGGKSPFIIFDDADVDQAVELAHFALFYNQGQCCCAGSRTYVHERVYDEFVEKAKARAMRRIVGDPFKSGVEQGPQIDPEQFEKVLRYIRSGIESNATLECGGDRLGNRGYFVQPTVFSDVKDDMLIAQDEIFGPVQSILKFKETDDVIRRANKTRFGLAAGVFTKSADTANTMMRALRAGTVWI 730
            AG PPGV+N+V GYGPTAGAA++SHMDVDK++FTG    GK+++E A KSNLK VTLELGGKSP I+F DAD+D AVE AH  +FY+QGQCC A SR +V E VYDEFV K+  RA + ++G+P   G+ QGPQID EQ +K+L  I SG +  A LECGG R GN+G+FVQPTVFS+V D+M IA++EIFGPVQ I+KFK  DDVI+RAN T +GLAAGVFTK  D A T+  AL+AG VW+
Sbjct:  212 AGFPPGVVNIVPGYGPTAGAAISSHMDVDKVAFTGSTQVGKLIKEAAGKSNLKRVTLELGGKSPCIVFADADLDIAVEFAHHGVFYHQGQCCVAASRIFVEESVYDEFVRKSVERAKKYVLGNPLTQGINQGPQIDKEQHDKILDLIESGKKEGAKLECGGGRWGNKGFFVQPTVFSNVTDEMRIAKEEIFGPVQQIMKFKSIDDVIKRANNTTYGLAAGVFTKDLDRAITVSSALQAGVVWV 454
The following BLAST results are available for this feature:
BLAST of CX295024 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
AL2B4_ARATH1.781e-11579.01Aldehyde dehydrogenase family 2 member B4, mitocho... [more]
AL2B7_ARATH2.943e-11076.95Aldehyde dehydrogenase family 2 member B7, mitocho... [more]
ALDH2_RAT4.885e-8964.61Aldehyde dehydrogenase, mitochondrial OS=Rattus no... [more]
ALDH2_PIG4.885e-8965.02Aldehyde dehydrogenase, mitochondrial OS=Sus scrof... [more]
ALDH2_MESAU4.885e-8964.61Aldehyde dehydrogenase, mitochondrial OS=Mesocrice... [more]
ALDH2_BOVIN1.421e-8864.20Aldehyde dehydrogenase, mitochondrial OS=Bos tauru... [more]
ALDH2_MOUSE2.425e-8864.20Aldehyde dehydrogenase, mitochondrial OS=Mus muscu... [more]
ALDH2_PONAB1.572e-8762.96Aldehyde dehydrogenase, mitochondrial OS=Pongo abe... [more]
ALDH2_HUMAN1.572e-8762.96Aldehyde dehydrogenase, mitochondrial OS=Homo sapi... [more]
AL1A1_RAT1.572e-8763.37Retinal dehydrogenase 1 OS=Rattus norvegicus GN=Al... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionC05065G06SK DroRLeaf1 Citrus clementina cDNA clone C05065G06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX295024 ID=CX295024; Name=CX295024; organism=Citrus clementina; type=EST; length=735bp
GGCTGGTCTTCCCCCCGGTGTTTTGAATGTAGTCTCTGGTTATGGTCCAA
CAGCTGGTGCAGCTCTTGCCAGCCATATGGATGTGGACAAGCTGTCTTTT
ACAGGACACTGTGATACTGGTAAGATTGTTCAGGAACTGGCTGCAAAAAG
CAACCTTAAGCCAGTAACCTTAGAACTTGGAGGGAAATCACCATTTATAA
TATTCGATGATGCTGATGTTGACCAGGCTGTGGAACTTGCACACTTTGCT
TTGTTCTATAATCAGGGACAATGTTGCTGTGCTGGATCTCGTACTTATGT
ACATGAGCGTGTATATGACGAGTTTGTAGAAAAAGCAAAGGCACGTGCAA
TGAGGCGTATTGTTGGCGATCCCTTCAAGAGTGGTGTTGAACAAGGTCCT
CAGATTGATCCAGAGCAATTTGAGAAGGTCCTTAGGTACATAAGGTCTGG
AATTGAAAGCAATGCAACCCTTGAGTGTGGAGGTGATAGATTGGGCAACA
GAGGCTACTTTGTTCAGCCAACTGTCTTCTCAGATGTTAAGGATGACATG
TTGATAGCACAGGATGAAATCTTTGGCCCGGTGCAATCCATCTTGAAGTT
CAAGGAAACTGATGACGTTATAAGAAGAGCAAATAAAACACGCTTTGGTC
TAGCTGCTGGGGTTTTCACCAAGAGTGCAGACACTGCTAACACCATGATG
CGTGCACTGAGAGCTGGGACTGTATGGATTACTGC
back to top