DY258475

Overview
NameDY258475
Unique NameDY258475
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length1299
Libraries
Library NameType
AbsDev1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of DY258475 vs. ExPASy Swiss-Prot
Match: ASO_TOBAC (L-ascorbate oxidase OS=Nicotiana tabacum GN=AAO PE=2 SV=1)

HSP 1 Score: 259.996 bits (663), Expect = 2.241e-68
Identity = 130/221 (58.82%), Postives = 156/221 (70.59%), Query Frame = 2
Query:    2 KSFSNKIFALMGSPKPPTNFHRRLTLLNTQNTINGFTKWAINNVSLNLPPTPYLGSIKYGLKDAFDQNGPPENFSNEYDVMKPPVNANTTLGSGVYMLGLNTTVDVILQNANAIRPNLSETHPWHLHGHDFWVLRRGEGKFTKEDEXXXXXXXXXXXXTAVIFPYGWTALRFVADNPRAWAFSLPY*APFPILEWVWFLLLVLKPVGNIPNQAVGCGLTGK 664
            KSFSNKIFALMGSPKPP   HRR+ LLNTQN I+G+TKWAINNVSL LP   YLGSI+YG+ +AFD   PP+NF  +YDV+K   N+N+T G+GVYML  NTT+D+ILQNANA+  ++SE HPWHLHGHDFWVL  GEGKF+++D             TAVIFP+GWTALRFV DNP  WAF   +  P   +         +  V  IP +A+ CGLTGK
Sbjct:  352 KSFSNKIFALMGSPKPPPQNHRRIILLNTQNKIDGYTKWAINNVSLVLPTQLYLGSIRYGI-NAFDTKPPPDNFPKDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGHDFWVLGYGEGKFSEKDVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHC-HIEPHLHMGMGVIFAEGVHLVKKIPKEALACGLTGK 570          
BLAST of DY258475 vs. ExPASy Swiss-Prot
Match: ASO_CUCPM (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo PE=1 SV=1)

HSP 1 Score: 256.914 bits (655), Expect = 1.897e-67
Identity = 128/227 (56.39%), Postives = 151/227 (66.52%), Query Frame = 2
Query:    2 KSFSNKIFALMGSPKPPTNFHRRLTLLNTQNTINGFTKWAINNVSLNLPPTPYLGSIKYGLKDAFDQNGPPENFSNEYDVMKPPVNANTTLGSGVYMLGLNTTVDVILQNANAIRPNLSETHPWHLHGHDFWVLRRGEGKFTKEDEXXXXXXXXXXXXTAVIFPYGWTALRFVADNPRAWAFSLPY*APFPILEWVWFLLLVLKPVGNIPNQAVGCGLTGKRFHEPP 682
            K+F+ +I A MGSPKPP  F+RR+ LLNTQN ING+ KWAIN+VSL LPPTPYLG++KY L  AFDQN PPE F  +YD+  PP N  T +G+GVY   +   VDVILQNAN ++ NLSETHPWHLHGHDFWVL  G+GKF+ E+E            T VIFPYGWTA+RFVADNP  WAF         +   V F   V K VG IP +A+ CG T K     P
Sbjct:  324 KNFTYRITAAMGSPKPPVKFNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGVYQFKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEK-VGRIPTKALACGGTAKSLINNP 549          
BLAST of DY258475 vs. ExPASy Swiss-Prot
Match: ASO_CUCMA (L-ascorbate oxidase OS=Cucurbita maxima GN=AAO PE=1 SV=2)

HSP 1 Score: 251.136 bits (640), Expect = 1.041e-65
Identity = 126/227 (55.51%), Postives = 149/227 (65.64%), Query Frame = 2
Query:    2 KSFSNKIFALMGSPKPPTNFHRRLTLLNTQNTINGFTKWAINNVSLNLPPTPYLGSIKYGLKDAFDQNGPPENFSNEYDVMKPPVNANTTLGSGVYMLGLNTTVDVILQNANAIRPNLSETHPWHLHGHDFWVLRRGEGKFTKEDEXXXXXXXXXXXXTAVIFPYGWTALRFVADNPRAWAFSLPY*APFPILEWVWFLLLVLKPVGNIPNQAVGCGLTGKRFHEPP 682
            K+F+ +I A MGSPKPP   +RR+ LLNTQN ING+ KWAIN+VSL LPPTPYLG++K+ L  AFDQN PPE F  +YD+  PP N  T +G+GVY   +   VDVILQNAN ++ NLSE HPWHLHGHDFWVL  G+GKFT E+E            T VIFPYGWTA+RFVADNP  WAF         +   V F   V K VG IP +A+ CG T K     P
Sbjct:  354 KNFTYRITAAMGSPKPPVKSNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVLGYGDGKFTAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEK-VGRIPTKALACGGTAKSLINNP 579          
BLAST of DY258475 vs. ExPASy Swiss-Prot
Match: ASO_CUCSA (L-ascorbate oxidase OS=Cucumis sativus PE=1 SV=1)

HSP 1 Score: 249.21 bits (635), Expect = 3.956e-65
Identity = 128/220 (58.18%), Postives = 145/220 (65.91%), Query Frame = 2
Query:    2 KSFSNKIFALMGSPKPPTNFHRRLTLLNTQNTINGFTKWAINNVSLNLPPTPYLGSIKYGLKDAFDQNGPPENFSNEYDVMKPPVNANTTLGSGVYMLGLNTTVDVILQNANAIRPNLSETHPWHLHGHDFWVLRRGEGKF-TKEDEXXXXXXXXXXXXTAVIFPYGWTALRFVADNPRAWAFSLPY*APFPILEWVWFLLLVLKPVGNIPNQAVGCGLT 658
            K+F+ +IFA MGSPKPP  ++RRL LLNTQN INGF KWAINNVSL LPPTPYL ++K  L  AF+QN PPE F   YD+  PP N  TT G+GVY   +  TVDVILQNAN + PN+SE HPWHLHGHDFWVL  GEGKF   EDE            T VIFPYGWTA+RFVADNP  WAF         +   V F   V   VG IP +A+ CG T
Sbjct:  359 KNFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGV-HMVGMIPPKALACGST 577          
BLAST of DY258475 vs. ExPASy Swiss-Prot
Match: LAC16_ARATH (Laccase-16 OS=Arabidopsis thaliana GN=LAC16 PE=2 SV=2)

HSP 1 Score: 90.8929 bits (224), Expect = 1.800e-17
Identity = 55/144 (38.19%), Postives = 74/144 (51.39%), Query Frame = 2
Query:  122 INNVSLNLPPTPYLGSIKYGLKDAFDQNGPPENFSNEYDVMKPP---VNANTTLGSGVYMLGLNTTVDVILQNANAIRPNLSETHPWHLHGHDFWVLRRGEGKFTKEDEXXXXXXXXXXXXTAVIFPY-GWTALRFVADNPRAW 541
            INNV+  +P T  L +  + +   F  + P +  SN YD   P    VNA T  G+ +Y L  N TV ++LQN   I   LS+ HP+HLHG +F+ + RG G F  E +              V  P  GWTA+RF+ADNP  W
Sbjct:  387 INNVTFTMPKTALLQAHFFNISGVFTDDFPAKP-SNPYDYTAPVKLGVNAATMKGTKLYRLPYNATVQIVLQNTAMI---LSDNHPFHLHGFNFFEVGRGLGNFNPEKDPKAFNLVDPVERNTVGVPAGGWTAIRFIADNPGVW 526          
BLAST of DY258475 vs. ExPASy Swiss-Prot
Match: LAC22_ORYSJ (Laccase-22 OS=Oryza sativa subsp. japonica GN=LAC22 PE=2 SV=2)

HSP 1 Score: 80.4925 bits (197), Expect = 2.433e-14
Identity = 47/141 (33.33%), Postives = 66/141 (46.81%), Query Frame = 2
Query:  122 INNVSLNLPPTPYLGSIKYGLKDAFDQNGPPENFSNEYDVMKPPVNANTTLGSGVYMLGLNTTVDVILQNANAIRPNLSETHPWHLHGHDFWVLRRGEGKFTKEDEXXXXXXXXXXXXTAVIFPY-GWTALRFVADNPRAW 541
            INNV+  +P TP L +  Y +   F ++ P             P N  T  G+ VY L  N +V V+LQ+   I P   E+HP HLHG +F+V+ +G G +                   +  P  GWTA+RF +DNP  W
Sbjct:  387 INNVTFIMPSTPILQAHYYNIPGVFTEDFPATPLHKFNYTGSGPKNLQTMNGTRVYRLPYNASVQVVLQDTGIISP---ESHPIHLHGFNFFVVGKGVGNYNPRTSPSTFNLIDPIERNTIGVPTGGWTAIRFRSDNPGVW 524          
BLAST of DY258475 vs. ExPASy Swiss-Prot
Match: LAC2_ORYSJ (Laccase-2 OS=Oryza sativa subsp. japonica GN=LAC2 PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 4.151e-14
Identity = 48/145 (33.10%), Postives = 74/145 (51.03%), Query Frame = 2
Query:  119 AINNVSLNLPPTPYLGSIKYGLKDAFDQN---GPPENFSNEYDVMKPPVNANTTLGSGVYMLGLNTTVDVILQNANAIRPNLSETHPWHLHGHDFWVLRRGEGKFTKEDEXXXXXXXXXXXXTAVIFPY-GWTALRFVADNPRAW 541
            ++NN++  +P T  L +  YG K  F  +    PP  F+  Y  +       T+LG+ +  +  N TV+++LQ+ N +     E+HP+HLHG++F+V+ RG G F    +              V  P  GWTA+RF ADNP  W
Sbjct:  383 SLNNITFVMPRTALLQAHYYGQKGVFAADFPDRPPARFN--YTGVPLTAGLGTSLGTRLSKIAYNATVELVLQDTNLLSV---ESHPFHLHGYNFFVVGRGVGNFDPAKDPAKYNLVDPPERNTVGVPAGGWTAIRFRADNPGVW 522          
BLAST of DY258475 vs. ExPASy Swiss-Prot
Match: LAC10_ARATH (Laccase-10 OS=Arabidopsis thaliana GN=LAC10 PE=2 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 1.208e-13
Identity = 47/142 (33.10%), Postives = 69/142 (48.59%), Query Frame = 2
Query:  119 AINNVSLNLPPTPYLGSIKYGLKDAFDQNGPPENFSNEYDVMKPPVNANTTLGSGVYMLGLNTTVDVILQNANAIRPNLSETHPWHLHGHDFWVLRRGEGKF-TKEDEXXXXXXXXXXXXTAVIFPYGWTALRFVADNPRAW 541
            AINN++  +P T  L +  + L   +  + P +         KPP N  T   + +Y L  N+TV V+LQ+   + P   E HP HLHG +F+V+  G G + +K+D             T  +   GW A+RF ADNP  W
Sbjct:  380 AINNITFKMPKTALLQAHYFNLTGIYTTDFPAKPRRVFDFTGKPPSNLATMKATKLYKLPYNSTVQVVLQDTGNVAP---ENHPIHLHGFNFFVVGLGTGNYNSKKDSNKFNLVDPVERNTVGVPSGGWAAIRFRADNPGVW 518          
BLAST of DY258475 vs. ExPASy Swiss-Prot
Match: LAC7_ORYSJ (Laccase-7 OS=Oryza sativa subsp. japonica GN=LAC7 PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.950e-13
Identity = 57/190 (30.00%), Postives = 83/190 (43.68%), Query Frame = 2
Query:   23 FALMGSPKP-----PTNFHRRLTLL---------NTQNTINGFT-KWAINNVSLNLPPTPYLGSIKYGLKDAFDQNGPPENFSNEYDVMKPPVNANTTLGSGVYMLGLNTTVDVILQNANAIRPNLSETHPWHLHGHDFWVLRRGEGKFTKEDEXXXXXXXXXXXXTAVIFPYGWTALRFVADNPRAWAF 547
            F L G P P     PT     +T+          +TQ++ +G +   A+N VS  LP    L   ++        N  P  ++ ++     P       G+ V  L  N+TV+++LQN  A     SE HP HLHG +F+VL +G G FT  +             T  +   GW  +RFVA+NP  W F
Sbjct:  343 FGLRGPPSPSAPAVPTKVDVNMTIELGLGQLPCDSTQSSCSGKSVAAAMNGVSFRLPSQMSLLEAQF--------NRTPGVYTADFPDAPQPSGTPMVEGTKVRRLKYNSTVEIVLQNPTAFP---SENHPIHLHGFNFFVLAQGLGNFTPGNVSGYNLVDPVSRNTLAVPTGGWAVIRFVANNPGMWFF 521          

HSP 2 Score: 23.0978 bits (48), Expect = 2.950e-13
Identity = 6/10 (60.00%), Postives = 8/10 (80.00%), Query Frame = 3
Query:  546 FHCHIEPHFP 575
            FHCH++ H P
Sbjct:  521 FHCHLDAHVP 530          
BLAST of DY258475 vs. ExPASy Swiss-Prot
Match: LAC11_ORYSJ (Putative laccase-11 OS=Oryza sativa subsp. japonica GN=LAC11 PE=5 SV=2)

HSP 1 Score: 74.3294 bits (181), Expect = 1.744e-12
Identity = 49/148 (33.11%), Postives = 72/148 (48.65%), Query Frame = 2
Query:  119 AINNVSLNLPPTPYLGS-IKYGLKDAFDQNGP-----PENFSNEYDVMKPPVNANTTLGSGVYMLGLNTTVDVILQNANAIRPNLSETHPWHLHGHDFWVLRRGEGKFTKEDEXXXXXXXXXXXXTAVIFP-YGWTALRFVADNPRAW 541
            ++NNVS  +P T  L +  +         N P     P N++       PP N   T G+ V  L  NTTV+V+LQ+ + +    +E+HP HLHG+DF+V+  G G +   ++              +  P  GW A+RFVADNP  W
Sbjct:  360 SMNNVSFVMPRTSLLQAHYQRRYNGVLAANFPAAPRTPFNYTGT-----PPNNTFVTHGTRVVPLSFNTTVEVVLQDTSILG---AESHPLHLHGYDFYVVGTGFGNYDASNDTAKYNLVDPVQRNTISVPTAGWVAIRFVADNPGVW 499          
The following BLAST results are available for this feature:
BLAST of DY258475 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 20
Match NameE-valueIdentityDescription
ASO_TOBAC2.241e-6858.82L-ascorbate oxidase OS=Nicotiana tabacum GN=AAO PE... [more]
ASO_CUCPM1.897e-6756.39L-ascorbate oxidase OS=Cucurbita pepo var. melopep... [more]
ASO_CUCMA1.041e-6555.51L-ascorbate oxidase OS=Cucurbita maxima GN=AAO PE=... [more]
ASO_CUCSA3.956e-6558.18L-ascorbate oxidase OS=Cucumis sativus PE=1 SV=1[more]
LAC16_ARATH1.800e-1738.19Laccase-16 OS=Arabidopsis thaliana GN=LAC16 PE=2 S... [more]
LAC22_ORYSJ2.433e-1433.33Laccase-22 OS=Oryza sativa subsp. japonica GN=LAC2... [more]
LAC2_ORYSJ4.151e-1433.10Laccase-2 OS=Oryza sativa subsp. japonica GN=LAC2 ... [more]
LAC10_ARATH1.208e-1333.10Laccase-10 OS=Arabidopsis thaliana GN=LAC10 PE=2 S... [more]
LAC7_ORYSJ2.950e-1330.00Laccase-7 OS=Oryza sativa subsp. japonica GN=LAC7 ... [more]
LAC11_ORYSJ1.744e-1233.11Putative laccase-11 OS=Oryza sativa subsp. japonic... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAI1DG10FM2 AbsDev1 Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY258475 ID=DY258475; Name=DY258475; organism=Citrus clementina; type=EST; length=1299bp
CAAATCATTCTCCAACAAAATATTTGCCCTAATGGGATCCCCCAAGCCTC
CCACAAATTTCCACCGTCGGCTCACCCTTCTTAACACTCAAAACACCATT
AATGGGTTCACTAAGTGGGCTATTAACAATGTGTCCCTGAATTTACCCCC
CACTCCTTACTTAGGATCCATCAAATATGGTCTTAAAGATGCTTTTGATC
AGAATGGTCCCCCCGAAAACTTCTCGAATGAGTATGACGTGATGAAACCT
CCTGTAAACGCTAATACAACTCTTGGAAGTGGGGTTTACATGCTTGGGTT
AAACACCACAGTTGATGTTATACTTCAAAATGCAAACGCGATAAGGCCAA
ACTTGAGTGAAACCCACCCTTGGCATTTGCATGGGCATGATTTTTGGGTA
TTACGGCGCGGAGAAGGGAAGTTTACAAAAGAAGATGAGAAGAAATTTAA
CTTGAACAATCCACCATTGAAAAATACTGCGGTGATATTCCCATATGGCT
GGACTGCTTTAAGATTTGTTGCTGATAATCCACGTGCTTGGGCATTTTCA
CTGCCATATTGAGCCCCATTTCCCATATTGGAATGGGTGTGGTTCTTGCT
CTTGGTGTTGAAACCCGTTGGTAATATTCCCAACCAGGCCGTGGGCTGTG
GTTTGACTGGGAAAAGGTTCCATGAACCCCCAAACAAAAATTGAGGATAC
CCAGTTCGCTTTACGAACTTTATCCCCTGGAGCTTCAATTGCTGTTTTGA
ATATTAAATTAATAAAATTTCCGAGTTAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAACCTACTTTTCTTGAAAAAGTTGGGATTAAAAACAACCCCTAGC
TTATAATTTGTTGCCCCCACCAGGTCCCACCCCCCCAAAAAAAAATTATT
TTTTCCCTCCCGCCTTATCTCCCCCATACACAAATCCCCTAAACACGGTA
CACCCCGTTTCCCCGGCCCCCCTCCCCCCGGTCCAAAAACTCCCGCAACC
CCCCATGCCCAAACACAAAAAAAATCCCACCCTCAATACACCCCCCACCC
AAACCCCCAAACCACTACTCACCCATTCACTCCTCCGATCTCACAAACCC
TTAATTATCGCAAAATAAAATATTCCCCCTGCAACCAACGAAAAAACACA
CTCACCCCACTCCCCCTCTCACTCACATATATACTCATACCATCCTCACC
TCCTCCCCCTCTCACCCCCCCACCTCCCCCCCCCCCCACTCCACCTAAAT
CTACCACCACACCACTAATCTATACCTTCACCCTCCCTCCCTCCAACAT
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