DY259875

BLAST of DY259875 vs. ExPASy Swiss-Prot
Match: PUS3_MOUSE (tRNA pseudouridine synthase 3 OS=Mus musculus GN=Pus3 PE=1 SV=2)

HSP 1 Score: 121.324 bits (303), Expect = 2.047e-29
Identity = 78/208 (37.50%), Postives = 107/208 (51.44%), Query Frame = 2

Query:   98 QSQLEALQNRVKELEADNAKLLSKLSLCRCEKIEDKVHDGVEDGTTLVEENRKTESXXXXXXXXXXXXXXXXTLNHHFKRYVALKVMYFGQRFYGFASDGNMEPTVESEIFKALEKTRLLVADKKESHYSRCGRTDKGVSSVGQVIAIYLRSNIKECNTNNASMGETVQEE--QIEDEIDYVRVLNRVLPKDIRVMGWSPAPTDFSAR 715
            ++Q+E L NRVKELE +  +L  K                        + N   +S                  + H +R+VALK+ Y G  + GFAS  N   T+E ++F+AL KTR LV  ++ S+Y RCGRTDKGVS+ GQVI++ LRS      T+  S    ++ E   +  EI Y  +LNRVLP DIRV+ W+P    FSAR
Sbjct:    7 RNQIEKLLNRVKELEQEVERLKKKKE----------------------QANNIKDSSIRENSLGSGKAKRAFDFSAHGRRHVALKIAYLGWGYQGFASQENTSNTIEEKLFEALTKTR-LVESRQTSNYHRCGRTDKGVSAFGQVISLDLRSQFP---TSRDSEDSNLKHEADDLAKEIRYTHILNRVLPADIRVLAWAPVEPSFSAR 188          

HSP 2 Score: 29.6462 bits (65), Expect = 2.047e-29
Identity = 11/20 (55.00%), Postives = 14/20 (70.00%), Query Frame = 1

Query:  718 MESAGKKFVGDHDFQTFCKM 777
            M  A +K+VG HDF+  CKM
Sbjct:  210 MNYAAQKYVGTHDFRNLCKM 229          

BLAST of DY259875 vs. ExPASy Swiss-Prot
Match: PUS3_BOVIN (tRNA pseudouridine synthase 3 OS=Bos taurus GN=PUS3 PE=2 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 2.665e-29
Identity = 65/131 (49.62%), Postives = 91/131 (69.47%), Query Frame = 2

Query:  326 HFKRYVALKVMYFGQRFYGFASDGNMEPTVESEIFKALEKTRLLVADKKESHYSRCGRTDKGVSSVGQVIAIYLRSNI-KECNTNNASMGETVQEEQIEDEIDYVRVLNRVLPKDIRVMGWSPAPTDFSAR 715
            H +R+VALK+ Y G  + GFAS  N   T+E ++F+AL KTR LV +++ S+Y RCGRTDKGVS+ GQVI++ LRS+I K  ++ + ++   V +  +  EI Y  +LNRVLP DIRV+ W+P  T FSAR
Sbjct:   60 HGQRHVALKIAYLGWGYQGFASQENTSNTIEEKLFEALTKTR-LVENRQTSNYHRCGRTDKGVSAFGQVISLDLRSHIPKGRDSEHFNLKNEVND--VATEIRYTHILNRVLPPDIRVLAWAPVETSFSAR 187          

HSP 2 Score: 29.6462 bits (65), Expect = 2.665e-29
Identity = 11/20 (55.00%), Postives = 14/20 (70.00%), Query Frame = 1

Query:  718 MESAGKKFVGDHDFQTFCKM 777
            M  A +K+VG HDF+  CKM
Sbjct:  209 MNYAAQKYVGTHDFRNLCKM 228          

BLAST of DY259875 vs. ExPASy Swiss-Prot
Match: PUS3_HUMAN (tRNA pseudouridine synthase 3 OS=Homo sapiens GN=PUS3 PE=1 SV=3)

HSP 1 Score: 116.316 bits (290), Expect = 3.729e-28
Identity = 70/206 (33.98%), Postives = 109/206 (52.91%), Query Frame = 2

Query:   98 QSQLEALQNRVKELEADNAKLLSKLSLCRCEKIEDKVHDGVEDGTTLVEENRKTESXXXXXXXXXXXXXXXXTLNHHFKRYVALKVMYFGQRFYGFASDGNMEPTVESEIFKALEKTRLLVADKKESHYSRCGRTDKGVSSVGQVIAIYLRSNIKECNTNNASMGETVQEEQIEDEIDYVRVLNRVLPKDIRVMGWSPAPTDFSAR 715
            ++Q E L  RV+ELE +  +L       + E+ ++K    + + +    + ++                     + H +R+VAL++ Y G  + GFAS  N   T+E ++F+AL KTR LV  ++ S+Y RCGRTDKGVS+ GQVI++ LRS       ++       +     +EI Y  +LNRVLP DIR++ W+P    FSAR
Sbjct:    8 RNQTEKLLKRVRELEQEVQRL-------KKEQAKNKEDSNIRENSAGAGKTKRA-----------------FDFSAHGRRHVALRIAYMGWGYQGFASQENTNNTIEEKLFEALTKTR-LVESRQTSNYHRCGRTDKGVSAFGQVISLDLRSQFPR-GRDSEDFNVKEEANAAAEEIRYTHILNRVLPPDIRILAWAPVEPSFSAR 187          

HSP 2 Score: 30.4166 bits (67), Expect = 3.729e-28
Identity = 11/20 (55.00%), Postives = 15/20 (75.00%), Query Frame = 1

Query:  718 MESAGKKFVGDHDFQTFCKM 777
            M+ A +K+VG HDF+  CKM
Sbjct:  209 MDYAAQKYVGTHDFRNLCKM 228          

BLAST of DY259875 vs. ExPASy Swiss-Prot
Match: TG124_CAEEL (Probable tRNA pseudouridine synthase tag-124 OS=Caenorhabditis elegans GN=tag-124 PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 2.076e-17
Identity = 52/136 (38.24%), Postives = 74/136 (54.41%), Query Frame = 2

Query:  326 HFKRYVALKVMYFGQRFYGFASDGNMEPTVESEIFKALEKTRLLVADKKESHYSRCGRTDKGVSSVGQVIAIYLRSNIKECNTNNASMGETVQEEQIED------EIDYVRVLNRVLPKDIRVMGWSPAPTDFSAR 715
            H +R +A++  Y G    G     + + TVE+ I +AL KT L + D  +  +SRCGRTDKGVS+  Q  A+ +RS       +     ++ QE Q  D      E+ YV++LN VLPK IRV  W+P    F+AR
Sbjct:   27 HPRRKIAIQFFYLGWEHDGLVQQPHTQNTVENHIMQALIKTHL-IEDWTKCDFSRCGRTDKGVSAFKQTAAMVVRSLCP--GDSGVFWSDSTQEHQKVDYKASGEELPYVKMLNGVLPKTIRVFAWAPVAQTFNAR 159          

HSP 2 Score: 25.0238 bits (53), Expect = 2.076e-17
Identity = 8/20 (40.00%), Postives = 12/20 (60.00%), Query Frame = 1

Query:  718 MESAGKKFVGDHDFQTFCKM 777
            M    +  VG+HDF  FC++
Sbjct:  181 MRQGAELLVGEHDFSNFCQI 200          

BLAST of DY259875 vs. ExPASy Swiss-Prot
Match: PUS3_YEAST (tRNA pseudouridine synthase 3 OS=Saccharomyces cerevisiae GN=PUS3 PE=1 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 2.853e-16
Identity = 51/136 (37.50%), Postives = 75/136 (55.15%), Query Frame = 2

Query:  326 HFKRYVALKVMYFGQRFYGFASDGNMEP--TVESEIFKALEKTRLLVADKKESH-YSRCGRTDKGVSSVGQVIAIYLRSNIKECNTNNASMGETVQEEQIED--EIDYVRVLNRVLPKDIRVMG-WSPAPTDFSAR 715
            H  R++AL+  Y G  + G A      P  TVE  I +A+ K +L+ +   + + +SRCGRTDKGVS++ QVI++ +RSN+           +  Q +   D  EI YV VLN++LP DIR+       P +F AR
Sbjct:   89 HNTRFIALRFAYLGWNYNGLAVQKEYTPLPTVEGTILEAMNKCKLVPSMVLQDYKFSRCGRTDKGVSAMNQVISLEVRSNLT----------DEEQRDPTNDSREIPYVHVLNQLLPDDIRISAVCLRPPPNFDAR 214          

HSP 2 Score: 29.6462 bits (65), Expect = 2.853e-16
Identity = 11/24 (45.83%), Postives = 15/24 (62.50%), Query Frame = 1

Query:  718 MESAGKKFVGDHDFQTFCKMGSTK 789
            M  A   FVG+ DF+ FCK+  +K
Sbjct:  236 MSKAASYFVGERDFRNFCKLDGSK 259          

BLAST of DY259875 vs. ExPASy Swiss-Prot
Match: YL85_SCHPO (Putative tRNA pseudouridine synthase C25B8.05 OS=Schizosaccharomyces pombe GN=SPAC25B8.05 PE=2 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 3.707e-16
Identity = 67/214 (31.31%), Postives = 98/214 (45.79%), Query Frame = 2

Query:   80 ELISSLQSQLEALQNRVKELEADNAKLLSKLSLCRCEKIEDKVHDGVEDGTTLVEENRKTESXXXXXXXXXXXXXXXXTLNHHFKRYVALKVMYFGQRFYGFAS--DGNMEPTVESEIFKALEKTRLLVADKKESHYSRCGRTDKGVSSVGQVIAIYLRSNIKECNTNNASMGETVQEEQIEDEIDYVRVLNRVLPKDIRVMGWSPAPTDFSAR 715
            EL   LQ    +L N  KE+E  N  + +K  L    +IE + H   E    LV ++   ++                T+      +VA K  Y G  + G A   +    PTVE ++F+AL +  L + D     +SRCGRTDKGVS++GQVI++ +R + +                     I Y  VLNR+LP DIR+ G++  P  F AR
Sbjct:   20 ELDGKLQKNTNSLPN--KEIEGKNEVIKNKNKLDNGNEIEPQ-HLSQE----LVLQHALQDASSLPKLKPVRPFQLEKTV------FVAFKFAYCGWNYNGLAYQLEPTPLPTVEGKVFEALLRAHL-ITDPSSCSFSRCGRTDKGVSAMGQVISLNVRHSERR-------------------PIIYCDVLNRLLPADIRITGYAFPPEGFDAR 200          

HSP 2 Score: 30.0314 bits (66), Expect = 3.707e-16
Identity = 10/24 (41.67%), Postives = 17/24 (70.83%), Query Frame = 1

Query:  718 MESAGKKFVGDHDFQTFCKMGSTK 789
            M  A   ++G+HDF+ FCK+ ++K
Sbjct:  226 MNEAAALYLGEHDFRNFCKIDASK 249          

BLAST of DY259875 vs. ExPASy Swiss-Prot
Match: TRUA_METST (tRNA pseudouridine synthase A OS=Methanosphaera stadtmanae (strain DSM 3091) GN=truA PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 2.892e-11
Identity = 43/127 (33.86%), Postives = 61/127 (48.03%), Query Frame = 2

Query:  335 RYVALKVMYFGQRFYGFASDGNMEPTVESEIFKALEKTRLLVADKKESHYSRCGRTDKGVSSVGQVIAIYLRSNIKECNTNNASMGETVQEEQIEDEIDYVRVLNRVLPKDIRVMGWSPAPTDFSAR 715
            R VALK+ Y G  F+G+    N   TVE E+ +  ++T + + D   +HYS  GRTDKGV S G VI+                         I DE  ++  LN +LP DI+++G +  P  F  R
Sbjct:    2 RRVALKIAYIGSNFHGYQRQPNYR-TVEGELLRVFKETNI-IEDTWTAHYSVAGRTDKGVHSTGNVISF------------------------ITDEDIHINQLNGLLPDDIKIIGEARVPYGFKVR 102          

HSP 2 Score: 21.9422 bits (45), Expect = 2.892e-11
Identity = 8/19 (42.11%), Postives = 12/19 (63.16%), Query Frame = 1

Query:  718 MESAGKKFVGDHDFQTFCK 774
            M  A + F+G H+F+ F K
Sbjct:  128 MHVAMESFIGKHNFRNFSK 146          

BLAST of DY259875 vs. ExPASy Swiss-Prot
Match: TRUA_THESM (tRNA pseudouridine synthase A OS=Thermococcus sibiricus (strain MM 739 / DSM 12597) GN=truA PE=3 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 4.881e-11
Identity = 44/127 (34.65%), Postives = 65/127 (51.18%), Query Frame = 2

Query:  341 VALKVMYFGQRFYGFASDGNMEPTVESEIFKALEKTRLLVADKKESHYSRCGRTDKGVSSVGQVIAIYLRSNIKECNTNNASMGETVQEEQIEDEIDYVRVLNRVLPKDIRVMGWSPAPTDFSARLW 721
            +ALK+ Y G +FYGF    ++  TVE E+ K L+K  + + D KE+++    RTD+GVS++G VIA          NT    + E              R+LN  L +DI ++G +  P DF  R W
Sbjct:    3 IALKIAYDGTKFYGFQRQPDLR-TVEGELIKVLKKLGI-IEDVKEANFKGASRTDRGVSALGNVIAF---------NTAKPELAE-------------ARILNHHL-RDIWILGKAEVPEDFHPRFW 104          

HSP 2 Score: 21.9422 bits (45), Expect = 4.881e-11
Identity = 6/20 (30.00%), Postives = 13/20 (65.00%), Query Frame = 1

Query:  718 MESAGKKFVGDHDFQTFCKM 777
            +++  + F+G HDF  F ++
Sbjct:  124 LKACAEAFLGRHDFSNFARL 143