DY260437

Overview
NameDY260437
Unique NameDY260437
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length1252
Libraries
Library NameType
Fruit-TFcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig1530 contig Ccv1_Contig1530:1..1252. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DY260437 vs. ExPASy Swiss-Prot
Match: SRG1_ARATH (Protein SRG1 OS=Arabidopsis thaliana GN=SRG1 PE=2 SV=1)

HSP 1 Score: 153.68 bits (387), Expect = 2.153e-36
Identity = 71/107 (66.36%), Postives = 88/107 (82.24%), Query Frame = 2 
Query:    2 EVYSMELKSLAMNLISKMGKVLNIKDEELREFFDN--GFQSMRMNYYPPCPQPEKVMGLTPHSDGSALTILLQINEVEGLQIKNDGKWIPITPLPNAFIVNIGDTLE 316
            E+YS E++S+A  LI+KM + L IK EEL + FD+    QSMRMNYYPPCPQP++V+GLTPHSD   LT+L+Q+N+VEGLQIK DGKW+P+ PLPNAFIVNIGD LE
Sbjct:  172 EMYSSEVQSVAKILIAKMARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVEGLQIKKDGKWVPVKPLPNAFIVNIGDVLE 278

HSP 2 Score: 79.7221 bits (195), Expect = 3.938e-14
Identity = 42/96 (43.75%), Postives = 64/96 (66.67%), Query Frame = 3 
Query:  243 RTMENGFLSHRCQMLSLSTSETH*SIEHRAIVNSIQERLSIATFYTKRLDGEIYPASSLISEKTPALFRRVTVEEYFRSRFARELRGKSHLDDLRI 530
            + + N F+ +   +L + T+ T+ SIEHR +VNS +ERLSIATF+   +  E+ PA SL+  +  A F+R+T++EY    F+R L GK++LD LRI
Sbjct:  263 KPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNVGMYKEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358
[Close]
BLAST of DY260437 vs. ExPASy Swiss-Prot
Match: GAOX2_ORYSJ (Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. japonica GN=20ox2 PE=1 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 3.690e-23
Identity = 46/101 (45.54%), Postives = 69/101 (68.32%), Query Frame = 2 
Query:    8 YSMELKSLAMNLISKMGKVLNIKDEELREFFDNGFQSMRMNYYPPCPQPEKVMGLTPHSDGSALTILLQINEVEGLQIKNDGKWIPITPLPNAFIVNIGDT 310
            Y  E+K L++ ++  +   L ++    REFF +    MR NYYPPCP+PE+ +G  PH D +ALTILLQ ++V GL++  DG+W P++P+P A ++NIGDT
Sbjct:  192 YCEEMKELSLTIMELLELSLGVERGYYREFFADSSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQ-DDVGGLEVLVDGEWRPVSPVPGAMVINIGDT 291

HSP 2 Score: 28.1054 bits (61), Expect = 3.690e-23
Identity = 14/31 (45.16%), Postives = 18/31 (58.06%), Query Frame = 3 
Query:  324 HRAIVNSIQERLSIATFYTKRLDGEIYPASS 416
            HRA+VN  +ER S+A F   R D  + P  S
Sbjct:  305 HRAVVNQRRERRSLAFFLCPREDRVVRPPPS 335
[Close]
BLAST of DY260437 vs. ExPASy Swiss-Prot
Match: GAOX2_ORYSI (Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. indica GN=20ox2 PE=1 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 3.690e-23
Identity = 46/101 (45.54%), Postives = 69/101 (68.32%), Query Frame = 2 
Query:    8 YSMELKSLAMNLISKMGKVLNIKDEELREFFDNGFQSMRMNYYPPCPQPEKVMGLTPHSDGSALTILLQINEVEGLQIKNDGKWIPITPLPNAFIVNIGDT 310
            Y  E+K L++ ++  +   L ++    REFF +    MR NYYPPCP+PE+ +G  PH D +ALTILLQ ++V GL++  DG+W P++P+P A ++NIGDT
Sbjct:  192 YCEEMKELSLTIMELLELSLGVERGYYREFFADSSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQ-DDVGGLEVLVDGEWRPVSPVPGAMVINIGDT 291

HSP 2 Score: 28.1054 bits (61), Expect = 3.690e-23
Identity = 14/31 (45.16%), Postives = 18/31 (58.06%), Query Frame = 3 
Query:  324 HRAIVNSIQERLSIATFYTKRLDGEIYPASS 416
            HRA+VN  +ER S+A F   R D  + P  S
Sbjct:  305 HRAVVNQRRERRSLAFFLCPREDRVVRPPPS 335
[Close]
BLAST of DY260437 vs. ExPASy Swiss-Prot
Match: GAOX1_ARATH (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=20ox1 PE=2 SV=2)

HSP 1 Score: 92.0485 bits (227), Expect = 9.271e-21
Identity = 44/101 (43.56%), Postives = 64/101 (63.37%), Query Frame = 2 
Query:    8 YSMELKSLAMNLISKMGKVLNIKDEELREFFDNGFQSMRMNYYPPCPQPEKVMGLTPHSDGSALTILLQINEVEGLQIKNDGKWIPITPLPNAFIVNIGDT 310
            Y   + SL++ ++  +G  L +K +  REFF+     MR+NYYPPC +P+  +G  PH D ++LTIL Q + V GLQ+  + +W  I P P AF+VNIGDT
Sbjct:  190 YCEAMSSLSLKIMELLGLSLGVKRDYFREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQ-DHVNGLQVFVENQWRSIRPNPKAFVVNIGDT 289

HSP 2 Score: 30.4166 bits (67), Expect = 9.271e-21
Identity = 16/46 (34.78%), Postives = 25/46 (54.35%), Query Frame = 3 
Query:  297 TSETH*SIEHRAIVNSIQERLSIATFYTKRLDGEIYPASSLISEKT 434
            +++ + S  HRA+VNS  ER S+A F   + D  + P   L+   T
Sbjct:  294 SNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELLDSIT 339
[Close]
BLAST of DY260437 vs. ExPASy Swiss-Prot
Match: GAO1B_WHEAT (Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 1.570e-20
Identity = 43/101 (42.57%), Postives = 63/101 (62.38%), Query Frame = 2 
Query:    8 YSMELKSLAMNLISKMGKVLNIKDEELREFFDNGFQSMRMNYYPPCPQPEKVMGLTPHSDGSALTILLQINEVEGLQIKNDGKWIPITPLPNAFIVNIGDT 310
            Y  E+  L++ ++  +G+ L +     R FF+     MR+NYYPPC +P + +G  PH D ++LTIL Q N V GLQ+  +G+W  I P  +AF+VNIGDT
Sbjct:  167 YCSEMSRLSLEIMEVLGESLGVGRAHYRRFFEGNDSIMRLNYYPPCQRPMETLGTGPHCDPTSLTILHQDN-VGGLQVHTEGRWRSIRPRADAFVVNIGDT 266

HSP 2 Score: 30.4166 bits (67), Expect = 1.570e-20
Identity = 14/41 (34.15%), Postives = 21/41 (51.22%), Query Frame = 3 
Query:  324 HRAIVNSIQERLSIATFYTKRLDGEIYPASSLISEKTPALF 446
            HRA+VNS   R S+A F    +D  + P  +L+    P  +
Sbjct:  280 HRAVVNSKVPRKSLAFFLCPEMDKVVAPPGTLVDAANPRAY 320
[Close]
BLAST of DY260437 vs. ExPASy Swiss-Prot
Match: GAO1A_WHEAT (Gibberellin 20 oxidase 1-A OS=Triticum aestivum GN=GA20ox1A PE=2 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 1.570e-20
Identity = 43/101 (42.57%), Postives = 63/101 (62.38%), Query Frame = 2 
Query:    8 YSMELKSLAMNLISKMGKVLNIKDEELREFFDNGFQSMRMNYYPPCPQPEKVMGLTPHSDGSALTILLQINEVEGLQIKNDGKWIPITPLPNAFIVNIGDT 310
            Y  E+  L++ ++  +G+ L +     R FF+     MR+NYYPPC +P + +G  PH D ++LTIL Q N V GLQ+  +G+W  I P  +AF+VNIGDT
Sbjct:  167 YCSEMSRLSLEIMEVLGESLGVGRAHYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDN-VGGLQVHTEGRWRSIRPRADAFVVNIGDT 266

HSP 2 Score: 30.8018 bits (68), Expect = 1.570e-20
Identity = 14/41 (34.15%), Postives = 21/41 (51.22%), Query Frame = 3 
Query:  324 HRAIVNSIQERLSIATFYTKRLDGEIYPASSLISEKTPALF 446
            HRA+VNS   R S+A F    +D  + P  +L+    P  +
Sbjct:  280 HRAVVNSRVPRKSLAFFLCPEMDKVVAPPGTLVDASNPRAY 320
[Close]
BLAST of DY260437 vs. ExPASy Swiss-Prot
Match: GAO1D_WHEAT (Gibberellin 20 oxidase 1-D OS=Triticum aestivum GN=GA20ox1D PE=1 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 2.042e-20
Identity = 43/101 (42.57%), Postives = 63/101 (62.38%), Query Frame = 2 
Query:    8 YSMELKSLAMNLISKMGKVLNIKDEELREFFDNGFQSMRMNYYPPCPQPEKVMGLTPHSDGSALTILLQINEVEGLQIKNDGKWIPITPLPNAFIVNIGDT 310
            Y  E+  L++ ++  +G+ L +     R FF+     MR+NYYPPC +P + +G  PH D ++LTIL Q N V GLQ+  +G+W  I P  +AF+VNIGDT
Sbjct:  167 YCSEMSRLSLEIMEVLGESLGVGRAHYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDN-VGGLQVHTEGRWRSIRPRADAFVVNIGDT 266

HSP 2 Score: 30.4166 bits (67), Expect = 2.042e-20
Identity = 14/41 (34.15%), Postives = 21/41 (51.22%), Query Frame = 3 
Query:  324 HRAIVNSIQERLSIATFYTKRLDGEIYPASSLISEKTPALF 446
            HRA+VNS   R S+A F    +D  + P  +L+    P  +
Sbjct:  280 HRAVVNSRVPRKSLAFFLCPEMDKVVAPPGTLVDAANPRAY 320
[Close]
BLAST of DY260437 vs. ExPASy Swiss-Prot
Match: FL3H_DIACA (Naringenin,2-oxoglutarate 3-dioxygenase OS=Dianthus caryophyllus GN=FHT PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 3.455e-20
Identity = 42/104 (40.38%), Postives = 65/104 (62.50%), Query Frame = 2 
Query:    2 EVYSMELKSLAMNLISKMGKVLNIKDEELREFFDNGFQSMRMNYYPPCPQPEKVMGLTPHSDGSALTILLQINEVEGLQIKNDG--KWIPITPLPNAFIVNIGD 307
            E YS +L +LA  L+  + + + ++ E L +   +  Q + +NYYP CPQP+  +GL  H+D   +T+LLQ ++V GLQ   DG   WI + P+P AF+VN+GD
Sbjct:  159 EEYSNKLMTLACTLLGVLSEAMGLELEALTKACVDMDQKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQ-DQVGGLQATRDGGKTWITVQPVPGAFVVNLGD 261

HSP 2 Score: 38.1206 bits (87), Expect = 3.455e-20
Identity = 21/64 (32.81%), Postives = 34/64 (53.12%), Query Frame = 3 
Query:  321 EHRAIVNSIQERLSIATFYTKRLDGEIYPASSLISEKTPALFRRVTVEEYFRSRFARELRGKSH 512
            +H+A+VNS   RLSIATF     D  +YP +    E +  +   +T  + +R + A++L    H
Sbjct:  275 DHQAVVNSECSRLSIATFQNPSPDATVYPLAIREGENS-IMEEPITFADLYRRKMAKDLEIARH 337
[Close]
BLAST of DY260437 vs. ExPASy Swiss-Prot
Match: FL3H_ARATH (Naringenin,2-oxoglutarate 3-dioxygenase OS=Arabidopsis thaliana GN=F3H PE=1 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 7.604e-20
Identity = 43/104 (41.35%), Postives = 63/104 (60.58%), Query Frame = 2 
Query:    2 EVYSMELKSLAMNLISKMGKVLNIKDEELREFFDNGFQSMRMNYYPPCPQPEKVMGLTPHSDGSALTILLQINEVEGLQIKND-GK-WIPITPLPNAFIVNIGD 307
            E YS  L SLA  L+  + + + ++ E L     +  Q + +NYYP CPQP+  +GL  H+D   +T+LLQ ++V GLQ   D GK WI + P+  AF+VN+GD
Sbjct:  158 EEYSERLMSLACKLLEVLSEAMGLEKESLTNACVDMDQKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQ-DQVGGLQATRDNGKTWITVQPVEGAFVVNLGD 260

HSP 2 Score: 40.0466 bits (92), Expect = 7.604e-20
Identity = 22/59 (37.29%), Postives = 31/59 (52.54%), Query Frame = 3 
Query:  321 EHRAIVNSIQERLSIATFYTKRLDGEIYPASSLISEKTPALFRRVTVEEYFRSRFAREL 497
            +H+A+VNS   RLSIATF     D  +YP      EK   L   +T  E ++ +  R+L
Sbjct:  274 DHQAVVNSNSSRLSIATFQNPAPDATVYPLKVREGEKA-ILEEPITFAEMYKRKMGRDL 331
[Close]
BLAST of DY260437 vs. ExPASy Swiss-Prot
Match: FL3H_MALDO (Naringenin,2-oxoglutarate 3-dioxygenase OS=Malus domestica PE=2 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 9.886e-20
Identity = 41/102 (40.20%), Postives = 64/102 (62.75%), Query Frame = 2 
Query:    8 YSMELKSLAMNLISKMGKVLNIKDEELREFFDNGFQSMRMNYYPPCPQPEKVMGLTPHSDGSALTILLQINEVEGLQ-IKNDGK-WIPITPLPNAFIVNIGD 307
            YS EL  LA  L+  + + + +  E L +   +  Q + +N+YP CPQP+  +GL  H+D   +T+LLQ ++V GLQ  ++DGK WI + P+  AF+VN+GD
Sbjct:  161 YSDELMGLACKLLGVLSEAMGLDTEALTKACVDMDQKVVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQ-DQVGGLQATRDDGKTWITVQPVEGAFVVNLGD 261

HSP 2 Score: 41.5874 bits (96), Expect = 9.886e-20
Identity = 22/59 (37.29%), Postives = 34/59 (57.63%), Query Frame = 3 
Query:  321 EHRAIVNSIQERLSIATFYTKRLDGEIYPASSLISEKTPALFRRVTVEEYFRSRFAREL 497
            +H+A+VNS   RLSIATF     +  +YP S    EK P L   +T  E ++ + +++L
Sbjct:  275 DHQAVVNSNSSRLSIATFQNPAQEAIVYPLSVREGEK-PILEAPITYTEMYKKKMSKDL 332
The following BLAST results are available for this feature:
BLAST of DY260437 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 75
Match NameE-valueIdentityDescription
SRG1_ARATH2.153e-3666.36Protein SRG1 OS=Arabidopsis thaliana GN=SRG1 PE=2 ... [more]
GAOX2_ORYSJ3.690e-2345.54Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. ja... [more]
GAOX2_ORYSI3.690e-2345.54Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. in... [more]
GAOX1_ARATH9.271e-2143.56Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana G... [more]
GAO1B_WHEAT1.570e-2042.57Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN... [more]
GAO1A_WHEAT1.570e-2042.57Gibberellin 20 oxidase 1-A OS=Triticum aestivum GN... [more]
GAO1D_WHEAT2.042e-2042.57Gibberellin 20 oxidase 1-D OS=Triticum aestivum GN... [more]
FL3H_DIACA3.455e-2040.38Naringenin,2-oxoglutarate 3-dioxygenase OS=Dianthu... [more]
FL3H_ARATH7.604e-2041.35Naringenin,2-oxoglutarate 3-dioxygenase OS=Arabido... [more]
FL3H_MALDO9.886e-2040.20Naringenin,2-oxoglutarate 3-dioxygenase OS=Malus d... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAP12YH20FM1 Fruit-TF Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY260437 ID=DY260437; Name=DY260437; organism=Citrus clementina; type=EST; length=1252bp
GGAAGTTTACTCAATGGAGTTAAAATCTTTGGCCATGAACCTGATATCGA
AAATGGGAAAAGTTTTAAATATCAAAGATGAAGAATTGAGAGAATTCTTT
GACAATGGTTTCCAATCAATGAGGATGAACTATTACCCTCCATGTCCTCA
GCCAGAAAAGGTTATGGGCCTCACCCCTCATTCTGATGGCTCTGCTCTTA
CCATTCTGCTACAAATCAATGAAGTAGAAGGTCTCCAGATCAAGAACGAT
GGAAAATGGATTCCTATCACACCGCTGCCAAATGCTTTCATTGTCAACAT
CGGAGACACATTAGAGCATTGAGCATCGTGCAATTGTGAACTCTATACAA
GAAAGACTTTCCATTGCCACATTTTACACAAAGAGATTAGATGGTGAAAT
ATATCCAGCATCCAGTTTGATATCTGAAAAAACTCCTGCATTGTTTCGAA
GAGTGACGGTTGAAGAATACTTTAGGAGCCGATTTGCTCGTGAGCTTAGG
GGAAAATCTCACCTGGATGACCTGAGAATTCAACATGGTCGAGGCTAGCA
TAAATTATTTTGGATAGTTGCAGATTTGTGCCAGGGAATTTTCTGATGAT
AATTGTGCTTATTTTGGACCCGTGTCCTTTCATTTTTGGAATAAAATTTT
TGCCGGCCTTGTCAGTTTTTTATCAGATTTCCAAGACACATTTTCCCTTA
AAAAAAAAAAAAAAAAAAAAAAACCCCCACTTTCTTGGAAAAAGTTGGGA
TCAATAAAAAAGCCTTTGCCTTACCATTTTGTTGGCACTAAACGGGTCCA
CCATCCATACAAAAAAAAAACCATTTTTTTCCCCCCACCCCCAGAAACAC
CCCCCCATAGGTGATTCAACATAACCTGGCCACAACCTTTTTTCCGCCTC
CCCCCCCCGCGCCCGCCTCCCCAAAAACCTCAAATGTGACACTCCTCCCC
ACTAAAACAAACCTCTCTCTCACTCTCTGTAACTAAAACCCTCTTGCGTC
CTTCGACACACCAACAGCCAAACTTTCTCTCCATACACCGATAGAACACT
CACTCCCCTTCATCATTCAAAAAAAAACCTGCTCTTTCCTCAAATAGCCA
GACAACAACACCAACCCCAACGAGCGGCACTATACTATCACAAAGCATGG
CCACCGCACCCGCGCGCGCACACGCACACCACCCAACTCTACGCACCCCC
TCCCTCCTCATCGCTCGCTAACAATATAACCCCGACGTCAACCACCGGTC
TT
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