Development and characterization of SCAR markers linked to the citrus tristeza virus resistance gene from Poncirus trifoliata

Publication Overview
TitleDevelopment and characterization of SCAR markers linked to the citrus tristeza virus resistance gene from Poncirus trifoliata
AuthorsDeng Z, Huang S, Xiao S, Gmitter FJ
TypeJournal Article
Journal NameGenome
Volume40
Issue5
Year1997
Page(s)697-704
CitationDeng Z, Huang S, Xiao S, Gmitter FJ. Development and characterization of SCAR markers linked to the citrus tristeza virus resistance gene from Poncirus trifoliata. Genome. 1997 Oct; 40(5):697-704.

Abstract

Twelve new dominant randomly amplified polymorphic DNA (RAPD) fragments associated with a single dominant gene for resistance to citrus tristeza virus (CTV) were identified using bulked segregant analysis of an intergeneric backcross family. These and eight previously reported RAPDs were mapped in the resistance gene (Ctv) region; the resulting localized linkage map spans about 32cM, with nine close flanking markers within 2.5cM of Ctv. Seven of 20 RAPD fragments linked with the resistance gene were cloned and sequenced, and their sequences were used to design longer primers to develop sequence characterized amplified region (SCAR) markers that can be utilized reliably in marker-assisted selection, high-resolution mapping, and mapbased cloning of the resistance gene. All seven cloned RAPDs were converted successfully into SCARs by redesigning primers, optimizing PCR parameters (especially the annealing temperature), or digesting amplification products with restriction enzymes. Four of the seven remained dominant markers, displaying presence-absence polymorphism patterns; the other three detected restriction site changes or length variations and thus were transformed into codominant markers. Two genomic regions rich in variability were also detected by two codominant SCAR markers.
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
Thong DeeThong Deecultivar
USDA 17-40USDA 17-40population
PomeroyPomeroycultivar
Features
This publication contains information about 7 features:
Feature NameUniquenameType
SCAA10SCAA10genetic_marker
SCAD02SCAD02genetic_marker
SCAD08SCAD08genetic_marker
SCB11SCB11genetic_marker
SCO07SCO07genetic_marker
SCT08SCT08genetic_marker
SCX18SCX18genetic_marker
Properties
Additional details for this publication include:
Property NameValue
Journal AbbreviationGenome
Language Abbreng
Publication Date1997 Oct
Publication TypeJournal Article
KeywordsPoncirus trifoliata, genetic markers, genes, disease resistance, Citrus tristeza virus, chromosome mapping, linkage (genetics), random amplified polymorphic DNA technique, dominance, segregation, sequence characterized amplified region
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:3019252
PMID: PubMedPMID:18464859