Identification of closely related citrus cultivars with inter-simple sequence repeat markers

Publication Overview
TitleIdentification of closely related citrus cultivars with inter-simple sequence repeat markers
AuthorsFang D, Roose M
TypeJournal Article
Journal NameTheoretical and applied genetics
Volume95
Issue3
Year1997
Page(s)408-417
CitationFang D, Roose M. Identification of closely related citrus cultivars with inter-simple sequence repeat markers. Theoretical and applied genetics. 1997 Aug; 95(3):408-417.

Abstract

Inter-simple sequence repeat (ISSR) markers generated by 22 primers were tested for their ability to distinguish among samples from 94 trees of 68 citrus cultivars. Within each of the six cultivar groups studied most of these cultivars are so closely related that they are difficult to distinguish by other molecular-marker techniques. ISSR markers involve PCR amplification of DNA using a single primer composed of a microsatellite sequence anchored at the 3' or 5' end by 2-4 arbitrary, often degenerate, nucleotides. The amplification products were separated on non-denaturing polyacrylamide gels and detected by silver staining. ISSR banding profiles were very repeatable on duplicate samples. Different citrus species had very different fingerprint patterns. Within Citrus sinensis (L.) Osbeck and C. paradisi Macf., in which all cultivars have originated by the selection of mutants, ISSR markers distinguished 14 of 33 sweet orange and 1 of 7 grapefruit cultivars. Five of six lemon cultivars were discriminated by ISSR markers. Many differences were found among mandarin cultivars; however, all five satsuma cultivars analyzed had identical ISSR fingerprints. Four of five citrange cultivars were distinguishable, but 'Troyer' and 'Carrizo' had identical ISSR fingerprints. 'Kuharske Carrizo' citrange, which has better citrus nematode resistance than other 'Carrizo' citrange accessions, had unique ISSR fingerprints. Three ISSR markers that differentiated certain sweet orange cultivars were hybridized to Southern blots of sweet orange DNA digested with different restriction endonucleases. The sweet orange cultivars tested could be distinguished by these ISSR-derived RFLP markers. Moreover, one ISSR marker unique to 'Ruby' blood orange was observed in its progeny trees.
Stocks
This publication contains information about 65 stocks:
Stock NameUniquenameType
MinneolaMinneolaaccession
MurcottMurcottaccession
OlindaOlindaaccession
TroyerTroyeraccession
Atwood (old line)Atwood (old line)accession
BentonBentonaccession
C-32C-32accession
C-35C-35accession
CampbellCampbellaccession
Campbell nucellarCampbell nucellaraccession
Cara Cara pinkCara Cara pinkaccession
CarrizoCarrizoaccession
Carrizo (seedling)Carrizo (seedling)accession
CarterCarteraccession
Clementine AlgerianClementine Algerianaccession
Clementine MonrealClementine Monrealaccession
ClusterClusteraccession
Corrigated ThomsonCorrigated Thomsonaccession
Cutter nucellarCutter nucellaraccession
DeltaDeltaaccession
Dobashi Beni satsumaDobashi Beni satsumaaccession
Dream NucellarDream Nucellaraccession
Dry navelDry navelaccession
Eddy (old line)Eddy (old line)accession
Fisher (old line)Fisher (old line)accession

Pages

Features
This publication contains information about 46 features:
Feature NameUniquenameType
Unnamed_1Unnamed_1genetic_marker
Unnamed_10Unnamed_10genetic_marker
Unnamed_11Unnamed_11genetic_marker
Unnamed_12Unnamed_12genetic_marker
Unnamed_13Unnamed_13genetic_marker
Unnamed_14Unnamed_14genetic_marker
Unnamed_15Unnamed_15genetic_marker
Unnamed_16Unnamed_16genetic_marker
Unnamed_17Unnamed_17genetic_marker
Unnamed_18Unnamed_18genetic_marker
Unnamed_19Unnamed_19genetic_marker
Unnamed_2Unnamed_2genetic_marker
Unnamed_20Unnamed_20genetic_marker
Unnamed_21Unnamed_21genetic_marker
Unnamed_22Unnamed_22genetic_marker
Unnamed_23Unnamed_23genetic_marker
Unnamed_24Unnamed_24genetic_marker
Unnamed_25Unnamed_25genetic_marker
Unnamed_26Unnamed_26genetic_marker
Unnamed_27Unnamed_27genetic_marker
Unnamed_28Unnamed_28genetic_marker
Unnamed_29Unnamed_29genetic_marker
Unnamed_3Unnamed_3genetic_marker
Unnamed_30Unnamed_30genetic_marker
Unnamed_31Unnamed_31genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date1997 Aug
Language Abbreng
Journal AbbreviationTheor. appl. genet.
KeywordsCitrus sinensis, Citrus paradisi, Citrus limon, Citrus reticulata, Citroncirus webberi, Poncirus trifoliata, genetic markers, cultivar identification, DNA fingerprinting, restriction fragment length polymorphism, repetitive sequences, species differences
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:3274633