Molecular markers flanking citrus tristeza virus resistance gene from Poncirus trifoliata (L.) Raf

Publication Overview
TitleMolecular markers flanking citrus tristeza virus resistance gene from Poncirus trifoliata (L.) Raf
AuthorsMestre P, Asins M, Pina J, Carbonell E, Navarro L
TypeJournal Article
Journal NameTheoretical and applied genetics
Volume94
Issue3/4
Year1997
Page(s)458-464
CitationMestre P, Asins M, Pina J, Carbonell E, Navarro L. Molecular markers flanking citrus tristeza virus resistance gene from Poncirus trifoliata (L.) Raf. Theoretical and applied genetics. 1997 Mar; 94(3/4):458-464.

Abstract

Two segregating populations for citrus tristeza virus (CTV) resistance derived from Poncirus trifoliata var 'Flying Dragon' by self-pollination and pollination to Citrus medica L. var ethrog 'Arizona' were inoculated with a common CTV isolate. The presence of virus was checked by the Double Antibody Sandwich Enzyme-Linked Assay and Direct Tissue Blot Immunoassay at 3, 6, and 12 months after inoculation. Seven RAPDs were found linked to the CTV resistance gene by bulked segregant analysis. The closest linked RAPDs were cloned to obtain linked codominant RFLPs and to increase the precision of the genetic distance estimation. The CTV resistance gene seems to be located between cW18 and cK16. Differences in genetic distances among progenies are large and can be explained by genome-wide reduction in the recombination of progeny derived from male versus female gametes.
Featuremaps
This publication contains information about 2 maps:
Map Name
Citrus-FlyingDragon/Flying Dragon-RIL-1996
Citrus-Arizona/FlyingDragon-F1-1996
Stocks
This publication contains information about 4 stocks:
Stock NameUniquenameType
flying dragonflying dragoncultivar
ArizonaArizonacultivar
Citrus-Arizona/FlyingDragon-F1Citrus-Arizona/FlyingDragon-F1population
Citrus-FlyingDragon/Flying Dragon-RILCitrus-FlyingDragon/Flying Dragon-RILpopulation
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date1997 Mar
Language Abbreng
Journal AbbreviationTheor. appl. genet.
KeywordsPoncirus trifoliata, Citrus medica, intergeneric hybridization, disease resistance, Citrus tristeza virus, plant breeding, genetic markers, restriction fragment length polymorphism, chromosome mapping, isozymes, loci, linkage (genetics), random amplified polymorphic DNA technique, segregation
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:3239436