Developing fruit juice sac at 38 DAFB

Overview
Library NameDeveloping fruit juice sac at 38 DAFB
Unique NameDeveloping fruit juice sac at 38 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The juice sac tissue was dissected out and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
Array NameDeveloping fruit juice sac at 38 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
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Feature NameUnique NameType
CK933265CK933265EST
CK933266CK933266EST
CK933267CK933267EST
CK933268CK933268EST
CK933269CK933269EST
CK933270CK933270EST
CK933271CK933271EST
CK933272CK933272EST
CK933273CK933273EST
CK933274CK933274EST
CK933275CK933275EST
CK933276CK933276EST
CK933277CK933277EST
CK933278CK933278EST
CK933279CK933279EST
CK933280CK933280EST
CK933281CK933281EST
CK933282CK933282EST
CK933283CK933283EST
CK933284CK933284EST
CK933285CK933285EST
CK933286CK933286EST
CK933287CK933287EST
CK933288CK933288EST
CK933289CK933289EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stage38 DAFB fruit sample-collected June 17, 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The juice sac tissue was dissected out and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type