Ruby Orange Developing Flower cDNA Library UCRCS04-UCR

Overview
Library NameRuby Orange Developing Flower cDNA Library UCRCS04-UCR
Unique NameRuby Orange Developing Flower cDNA Library UCRCS04-UCR
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two trees with open-pollinated flowers in a mixed planting at UC Riverside Agricultural Operations were the source of tissue. Flower buds were collected by Federici (Roose lab) from January -March 2003 in four groups: 1) no white petals, 2) white petals visible, bud less than 0.5 cm, 3) bud more than 0.5 cm but not open, 4) newly opened flowers. Tissues were snap frozen and then stored at -80C until further processing. Fenton (Close lab) purified RNA by the phenol method described in J. Japanese Soc. Hort. Sci. 1996. 64 (4): 809-814, purified poly(A) mRNA using a PolyATtrack mRNA Isolation System IV (Promega), produced a primary cDNA library using a lambda ZAP XR cDNA Synthesis Kit (Stratagene), then mass-excised one million pfu from the primary library to produce a phagemid population. Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the Genomics Core Instrumentation Facility, University of California, Riverside (Choi, Kingan). Chromatogram files were downloaded by FTP by Close, then processed by Wanamaker (Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.
SNP Chip Base
Array NameRuby Orange Developing Flower cDNA Library UCRCS04-UCR
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
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Feature NameUnique NameType
CV884537CV884537EST
CV884538CV884538EST
CV884539CV884539EST
CV884540CV884540EST
CV884541CV884541EST
CV884542CV884542EST
CV884543CV884543EST
CV884544CV884544EST
CV884545CV884545EST
CV884546CV884546EST
CV884547CV884547EST
CV884548CV884548EST
CV884549CV884549EST
CV884550CV884550EST
CV884551CV884551EST
CV884552CV884552EST
CV884553CV884553EST
CV884554CV884554EST
CV884555CV884555EST
CV884556CV884556EST
CV884557CV884557EST
CV884558CV884558EST
CV884559CV884559EST
CV884560CV884560EST
CV884561CV884561EST

Pages

Properties
Property NameValue
Genbank library cultivarRuby
Genbank library dev stage20 year old trees
Genbank library noteVector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two trees with open-pollinated flowers in a mixed planting at UC Riverside Agricultural Operations were the source of tissue. Flower buds were collected by Federici (Roose lab) from January -March 2003 in four groups: 1) no white petals, 2) white petals visible, bud less than 0.5 cm, 3) bud more than 0.5 cm but not open, 4) newly opened flowers. Tissues were snap frozen and then stored at -80C until further processing. Fenton (Close lab) purified RNA by the phenol method described in J. Japanese Soc. Hort. Sci. 1996. 64 (4): 809-814, purified poly(A) mRNA using a PolyATtrack mRNA Isolation System IV (Promega), produced a primary cDNA library using a lambda ZAP XR cDNA Synthesis Kit (Stratagene), then mass-excised one million pfu from the primary library to produce a phagemid population. Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the Genomics Core Instrumentation Facility, University of California, Riverside (Choi, Kingan). Chromatogram files were downloaded by FTP by Close, then processed by Wanamaker (Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.
Genbank library tissue typeFlower
Flowertissue type