Mature Albedo

Overview
Library NameMature Albedo
Unique NameMature Albedo
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Peel/rind; Vector: pTriplEx2; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Albedo tissue was separated from the rest of the peel and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Features
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Feature NameUnique NameType
CB610797CB610797EST
CB610798CB610798EST
CB610799CB610799EST
CB610800CB610800EST
CB610801CB610801EST
CB610802CB610802EST
CB610803CB610803EST
CB610804CB610804EST
CB610805CB610805EST
CB610806CB610806EST
CB610807CB610807EST
CB610808CB610808EST
CB610809CB610809EST
CB610810CB610810EST
CB610811CB610811EST
CB610812CB610812EST
CB610813CB610813EST
CB610814CB610814EST
CB610815CB610815EST
CB610816CB610816EST
CB610817CB610817EST
CB610820CB610820EST
CB610822CB610822EST
CB610823CB610823EST
CB610824CB610824EST

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Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageMature fruit sample - collected January 2002
Genbank library noteOrgan: Peel/rind; Vector: pTriplEx2; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Albedo tissue was separated from the rest of the peel and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Pericarptissue type