Mature Albedo

Overview
Library NameMature Albedo
Unique NameMature Albedo
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Peel/rind; Vector: pTriplEx2; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Albedo tissue was separated from the rest of the peel and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
Array NameMature Albedo
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
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Feature NameUnique NameType
CB610825CB610825EST
CB610826CB610826EST
CB610827CB610827EST
CB610828CB610828EST
CB610829CB610829EST
CB610830CB610830EST
CB610831CB610831EST
CB610832CB610832EST
CB610833CB610833EST
CB610834CB610834EST
CB610835CB610835EST
CB610836CB610836EST
CB610837CB610837EST
CB610838CB610838EST
CB610839CB610839EST
CB610840CB610840EST
CB610841CB610841EST
CB610842CB610842EST
CB610843CB610843EST
CB610844CB610844EST
CB610845CB610845EST
CB610846CB610846EST
CB610847CB610847EST
CB610848CB610848EST
CB610849CB610849EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageMature fruit sample - collected January 2002
Genbank library noteOrgan: Peel/rind; Vector: pTriplEx2; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Albedo tissue was separated from the rest of the peel and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Pericarptissue type