Developing fruit albedo at 80 DAFB in pTriplEx2 vector

Overview
Library NameDeveloping fruit albedo at 80 DAFB in pTriplEx2 vector
Unique NameDeveloping fruit albedo at 80 DAFB in pTriplEx2 vector
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on July 29, 2003, between 8 to 9 am and stored at 4C. The albedo tissue was dissected out of developing fruit (80 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
Array NameDeveloping fruit albedo at 80 DAFB in pTriplEx2 vector
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
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Feature NameUnique NameType
CK937674CK937674EST
CK937675CK937675EST
CK937676CK937676EST
CK937677CK937677EST
CK937680CK937680EST
CK937681CK937681EST
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CK937685CK937685EST
CK937686CK937686EST
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CK937690CK937690EST
CK937691CK937691EST
CK937692CK937692EST
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CK937694CK937694EST
CK937695CK937695EST
CK937696CK937696EST
CK937697CK937697EST
CK937698CK937698EST
CK937699CK937699EST
CK937700CK937700EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageDeveloping fruit albedo-collected July 29, 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on July 29, 2003, between 8 to 9 am and stored at 4C. The albedo tissue was dissected out of developing fruit (80 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type