Developing fruit albedo at 80 DAFB in pTriplEx2 vector

Overview
Library NameDeveloping fruit albedo at 80 DAFB in pTriplEx2 vector
Unique NameDeveloping fruit albedo at 80 DAFB in pTriplEx2 vector
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on July 29, 2003, between 8 to 9 am and stored at 4C. The albedo tissue was dissected out of developing fruit (80 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Features
The following browser provides a quick view for new visitors. Use the searching mechanism to find specific features.
Feature NameUnique NameType
CK937726CK937726EST
CK937727CK937727EST
CK937728CK937728EST
CK937729CK937729EST
CK937730CK937730EST
CK937731CK937731EST
CK937733CK937733EST
CK937734CK937734EST
CK937735CK937735EST
CK937736CK937736EST
CK937737CK937737EST
CK937738CK937738EST
CK937739CK937739EST
CK937740CK937740EST
CK937741CK937741EST
CK937742CK937742EST
CK937743CK937743EST
CK937744CK937744EST
CK937745CK937745EST
CK937746CK937746EST
CK937747CK937747EST
CK937748CK937748EST
CK937749CK937749EST
CK937750CK937750EST
CK937751CK937751EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageDeveloping fruit albedo-collected July 29, 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on July 29, 2003, between 8 to 9 am and stored at 4C. The albedo tissue was dissected out of developing fruit (80 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type