Washington Navel orange cold acclimated flavedo & albedo cDNA library

Overview
Library NameWashington Navel orange cold acclimated flavedo & albedo cDNA library
Unique NameWashington Navel orange cold acclimated flavedo & albedo cDNA library
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the field at University of California, Riverside Agricultural Operations since 1983. The scion was Washington Navel orange and the rootstock Carizzo Citrange. Tissue from mature fruit was harvested at mid-day in January 2002 during a cold spell, when pre-dawn temperatures were approximately -2 to 2 degree C. Approximately 2 cm median sections of the rind were excised in the field from several fruits, then wrapped in aluminum foil and frozen quickly in dry ice. Total RNA was extracted using a phenol extraction procedure described in J. Japanaese Soc. Hort. Sci. (1996) 64:809-814. Poly(A) RNA was purified, a cDNA library was made, and 1 million primary lambda cDNA clones were in vivo excised to give a population of pBluescript SK(-) phagemids. All steps to this point were performed in the TJ Close lab at the University of California, Riverside (Fenton). Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3530 at the Arizona Genomics Institute, University of Arizona (Collura, Feuerbacher, Kim, Kudrna, Wing, Yu). Chromatogram files were transmitted to UC Riverside (by Yu), then processed at UC Riverside (by Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.
Features
The following browser provides a quick view for new visitors. Use the searching mechanism to find specific features.
Feature NameUnique NameType
CB293508CB293508EST
CB293510CB293510EST
CB293511CB293511EST
CB293512CB293512EST
CB293513CB293513EST
CB293514CB293514EST
CB293515CB293515EST
CB293516CB293516EST
CB293517CB293517EST
CB293518CB293518EST
CB293519CB293519EST
CB293520CB293520EST
CB293521CB293521EST
CB293522CB293522EST
CB293523CB293523EST
CB293524CB293524EST
CB293525CB293525EST
CB293527CB293527EST
CB293528CB293528EST
CB293529CB293529EST
CB293530CB293530EST
CB293531CB293531EST
CB293532CB293532EST
CB293533CB293533EST
CB293534CB293534EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel
Genbank library dev stageMature fruit
Genbank library noteVector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the field at University of California, Riverside Agricultural Operations since 1983. The scion was Washington Navel orange and the rootstock Carizzo Citrange. Tissue from mature fruit was harvested at mid-day in January 2002 during a cold spell, when pre-dawn temperatures were approximately -2 to 2 degree C. Approximately 2 cm median sections of the rind were excised in the field from several fruits, then wrapped in aluminum foil and frozen quickly in dry ice. Total RNA was extracted using a phenol extraction procedure described in J. Japanaese Soc. Hort. Sci. (1996) 64:809-814. Poly(A) RNA was purified, a cDNA library was made, and 1 million primary lambda cDNA clones were in vivo excised to give a population of pBluescript SK(-) phagemids. All steps to this point were performed in the TJ Close lab at the University of California, Riverside (Fenton). Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3530 at the Arizona Genomics Institute, University of Arizona (Collura, Feuerbacher, Kim, Kudrna, Wing, Yu). Chromatogram files were transmitted to UC Riverside (by Yu), then processed at UC Riverside (by Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.
Genbank library tissue typeRind containing flavedo and albedo
Pericarptissue type