Developing fruit albedo at 165 DAFB

Overview
Library NameDeveloping fruit albedo at 165 DAFB
Unique NameDeveloping fruit albedo at 165 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on October 22, 2003, between 8 to 9 am and stored at 4C. The albedo tissue was dissected out of developing fruit (165 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
Array NameDeveloping fruit albedo at 165 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
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Feature NameUnique NameType
CK938856CK938856EST
CK938857CK938857EST
CK938858CK938858EST
CK938859CK938859EST
CK938860CK938860EST
CK938861CK938861EST
CK938862CK938862EST
CK938863CK938863EST
CK938864CK938864EST
CK938865CK938865EST
CK938866CK938866EST
CK938867CK938867EST
CK938868CK938868EST
CK938869CK938869EST
CK938870CK938870EST
CK938871CK938871EST
CK938872CK938872EST
CK938873CK938873EST
CK938874CK938874EST
CK938875CK938875EST
CK938876CK938876EST
CK938877CK938877EST
CK938878CK938878EST
CK938879CK938879EST
CK938880CK938880EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageDeveloping fruit albedo-collected October 22, 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on October 22, 2003, between 8 to 9 am and stored at 4C. The albedo tissue was dissected out of developing fruit (165 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type