Developing fruit albedo at 165 DAFB

Overview
Library NameDeveloping fruit albedo at 165 DAFB
Unique NameDeveloping fruit albedo at 165 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on October 22, 2003, between 8 to 9 am and stored at 4C. The albedo tissue was dissected out of developing fruit (165 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
Array NameDeveloping fruit albedo at 165 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
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Feature NameUnique NameType
CK938528CK938528EST
CK938529CK938529EST
CK938530CK938530EST
CK938531CK938531EST
CK938532CK938532EST
CK938533CK938533EST
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CK938536CK938536EST
CK938537CK938537EST
CK938538CK938538EST
CK938539CK938539EST
CK938540CK938540EST
CK938541CK938541EST
CK938542CK938542EST
CK938543CK938543EST
CK938544CK938544EST
CK938545CK938545EST
CK938546CK938546EST
CK938547CK938547EST
CK938548CK938548EST
CK938549CK938549EST
CK938550CK938550EST
CK938551CK938551EST
CK938552CK938552EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageDeveloping fruit albedo-collected October 22, 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on October 22, 2003, between 8 to 9 am and stored at 4C. The albedo tissue was dissected out of developing fruit (165 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type