Developing fruit 24 DAFB

Overview
Library NameDeveloping fruit 24 DAFB
Unique NameDeveloping fruit 24 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 3, 2003, between 8 to 9 am and stored at 4C. The entire fruit was used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Features
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Feature NameUnique NameType
CK935455CK935455EST
CK935456CK935456EST
CK935457CK935457EST
CK935458CK935458EST
CK935459CK935459EST
CK935460CK935460EST
CK935461CK935461EST
CK935462CK935462EST
CK935463CK935463EST
CK935464CK935464EST
CK935465CK935465EST
CK935467CK935467EST
CK935468CK935468EST
CK935469CK935469EST
CK935470CK935470EST
CK935471CK935471EST
CK935472CK935472EST
CK935473CK935473EST
CK935474CK935474EST
CK935475CK935475EST
CK935476CK935476EST
CK935477CK935477EST
CK935478CK935478EST
CK935479CK935479EST
CK935480CK935480EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageYoung fruit sample-collected June 3 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 3, 2003, between 8 to 9 am and stored at 4C. The entire fruit was used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type