Developing fruit 24 DAFB

Overview
Library NameDeveloping fruit 24 DAFB
Unique NameDeveloping fruit 24 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 3, 2003, between 8 to 9 am and stored at 4C. The entire fruit was used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
Array NameDeveloping fruit 24 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
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Feature NameUnique NameType
CK936382CK936382EST
CK936383CK936383EST
CK936384CK936384EST
CK936385CK936385EST
CK936386CK936386EST
CK936387CK936387EST
CK936388CK936388EST
CK936389CK936389EST
CK936390CK936390EST
CK936391CK936391EST
CK936392CK936392EST
CK936393CK936393EST
CK936394CK936394EST
CK936395CK936395EST
CK936396CK936396EST
CK936397CK936397EST
CK936398CK936398EST
CK936399CK936399EST
CK936400CK936400EST
CK936401CK936401EST
CK936402CK936402EST
CK936403CK936403EST
CK936404CK936404EST
CK936405CK936405EST
CK936406CK936406EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageYoung fruit sample-collected June 3 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 3, 2003, between 8 to 9 am and stored at 4C. The entire fruit was used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type