Citrus glauca CRC3463 genome v1

Overview
Analysis NameCitrus glauca CRC3463 genome v1
MethodPacBio Hifi and CCS (Assembly with IPA and Hifiasm)
SourceUniversity of California Riverside
Date performed2023-05-24

 

CGD Accession: CGD23001

NCBI BioProject Accessions: primary haplotype, PRJNA924119; alternate haplotype, PRJNA924121

Citation:  Singh K, Huff M, Liu J, Park J-W, Rickman T, Keremane M, Krueger RR, Kunta M, Roose ML, Dardick C, et al. Chromosome-Scale, De Novo, Phased Genome Assemblies of Three Australian Limes: Citrus australasicaC. inodora, and C. glaucaPlants. 2024; 13(11):1460. https://doi.org/10.3390/plants13111460

Project Information:  The authors have kindly provided this data pre-publication according to the Ft. Lauderdale Accord. By accessing these data, you agree not to publish any articles containing analyses of genes or genomic data on a whole genome or chromosome scale prior to publication by the authors and/or collaborators of a comprehensive genome analysis such as identification of complete (whole genome) sets of genomic features such as genes, gene families, regulatory elements, repeat structures, GC content, or any other genome feature, and whole-genome- or chromosome- scale comparisons with other species.  Bulk download of the assembly files will become available once the data is published.  If you would like access to the data prior to that, please contact the corresponding author directly (Chandrika Ramadugu, chandram@ucr.edu).

Funding: USDA NIFA  ECDRE #2019-70016-29068 and #2020-70029-33201

 

Citrus glauca CRC3463 genome v1

TaxonomyCitrus glauca (Lindl.) Burkill 

Synonyms: Eremocitrus glauca (Lindl.) Swingle

Cultivar: 'Australian desert lime'  

Accession IDs:  IVNO 6206; CRC 3463PI 539717

The sample was collected from a single tree (inventory identifier IVNO 6206, planted in 1983) in the Givaudan Citrus Variety Collection, University of California Riverside (UCR). The accession is identified as CRC 3463 in the UCR system and PI 539717 in the USDA National Plant Germplasm System (NPGS).   Seeds from a related source (Eremocitrus glauca hybrid (Lindl.) Swingle, CRC 4105) were propagated and used for testing for field resistance/tolerance against citrus Huanglongbing (HLB) disease in an HLB endemic region of Florida, USA and found to be highly tolerant to the disease (Ramadugu et al., 2016). In subsequent experiments, C. glauca (CRC 3463) was also found to be highly tolerant to HLB (unpublished). A description of C. glauca is available here.  Propagative and genetic material from this accession is available from the USDA National Clonal Germplasm Repository for Citrus and Dates, Riverside, California subject to phytosanitary regulations. Note: this accession has not been sanitized.

C. glauca assembly metrics (combined haplotypes)

Number of scaffolds 166
Total bases 756 Mb
N50 36,986,125 b
Assembly BUSCO score (embryophtya_odb10) 97.6%
Annotation BUSCO score (embryophyta_odb10) 92.6%