DY270620

Overview
NameDY270620
Unique NameDY270620
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length1359
Libraries
Library NameType
Fruit-TFcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENO_METTP (Enolase OS=Methanosaeta thermophila (strain DSM 6194 / PT) GN=eno PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 9.680e-25
Identity = 54/79 (68.35%), Postives = 59/79 (74.68%), Query Frame = 2 
Query:   38 MAITITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPAL 274
            M++ I  + AR+I DSR NPTVEVDV T  G   RAAVPSGASTG YEALELRDGG  Y GKGV KAV NVN +IGP L
Sbjct:    1 MSLEIEKIHAREILDSRGNPTVEVDVWTCCGF-GRAAVPSGASTGTYEALELRDGGDRYDGKGVLKAVRNVNEVIGPKL 78

HSP 2 Score: 36.5798 bits (83), Expect = 9.680e-25
Identity = 23/55 (41.82%), Postives = 29/55 (52.73%), Query Frame = 3 
Query:  279 GKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            G D  +Q  +D  M++ +DGT N     K  LGAN  L VSLA  KA A    +P
Sbjct:   80 GMDVIDQRGVDQIMIE-MDGTPN-----KSNLGANAILGVSLAVAKAAASSLGMP 128
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENO_LACDB (Enolase OS=Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC BAA-365) GN=eno PE=3 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 1.259e-24
Identity = 52/82 (63.41%), Postives = 59/82 (71.95%), Query Frame = 2 
Query:   44 ITITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALRWQGP 289
            + I  V AR+IFDSR NPTVEV+VT S+G V RA VPSGASTG  EA+ELRDGGS   GKGV KAV+NVN  I   L+   P
Sbjct:    4 VVIENVHAREIFDSRGNPTVEVEVTLSNGVVGRAEVPSGASTGENEAVELRDGGSRLGGKGVMKAVNNVNTAINNELQGADP 85

HSP 2 Score: 40.817 bits (94), Expect = 1.259e-24
Identity = 27/55 (49.09%), Postives = 30/55 (54.55%), Query Frame = 3 
Query:  279 GKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            G DP  Q AID  M+ +LDGT N     K KLGAN  L  S+AT  A A  T  P
Sbjct:   82 GADPFNQPAIDKAMI-ELDGTPN-----KGKLGANAILGTSMATAVAAAKATHQP 130
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENO_LACDA (Enolase OS=Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081) GN=eno PE=3 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 1.259e-24
Identity = 52/82 (63.41%), Postives = 59/82 (71.95%), Query Frame = 2 
Query:   44 ITITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALRWQGP 289
            + I  V AR+IFDSR NPTVEV+VT S+G V RA VPSGASTG  EA+ELRDGGS   GKGV KAV+NVN  I   L+   P
Sbjct:    4 VVIENVHAREIFDSRGNPTVEVEVTLSNGVVGRAEVPSGASTGENEAVELRDGGSRLGGKGVMKAVNNVNTAINNELQGADP 85

HSP 2 Score: 40.817 bits (94), Expect = 1.259e-24
Identity = 27/55 (49.09%), Postives = 30/55 (54.55%), Query Frame = 3 
Query:  279 GKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            G DP  Q AID  M+ +LDGT N     K KLGAN  L  S+AT  A A  T  P
Sbjct:   82 GADPFNQPAIDKAMI-ELDGTPN-----KGKLGANAILGTSMATAVAAAKATHQP 130
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENO_RHILW (Enolase OS=Rhizobium leguminosarum bv. trifolii (strain WSM2304) GN=eno PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.639e-24
Identity = 49/75 (65.33%), Postives = 55/75 (73.33%), Query Frame = 2 
Query:   50 ITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPAL 274
            IT + AR+I DSR NPTVEVDV   DG + RAAVPSGASTG +EA+ELRDGG  YLGKGV KAV   N  I  A+
Sbjct:    4 ITDIIAREILDSRGNPTVEVDVYLEDGSMGRAAVPSGASTGAHEAVELRDGGKRYLGKGVQKAVDAANTEIFDAI 78

HSP 2 Score: 41.5874 bits (96), Expect = 1.639e-24
Identity = 26/55 (47.27%), Postives = 31/55 (56.36%), Query Frame = 3 
Query:  279 GKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            G D   Q  IDN M++ LDGT N     K +LGAN  L VSLA  KA A  + +P
Sbjct:   80 GIDAENQIQIDNIMIE-LDGTPN-----KSRLGANAILGVSLAVAKAAAQASGLP 128
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENO_RHIL3 (Enolase OS=Rhizobium leguminosarum bv. viciae (strain 3841) GN=eno PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.639e-24
Identity = 49/75 (65.33%), Postives = 55/75 (73.33%), Query Frame = 2 
Query:   50 ITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPAL 274
            IT + AR+I DSR NPTVEVDV   DG + RAAVPSGASTG +EA+ELRDGG  YLGKGV KAV   N  I  A+
Sbjct:    4 ITDIIAREILDSRGNPTVEVDVYLEDGSMGRAAVPSGASTGAHEAVELRDGGKRYLGKGVEKAVEAANTEIFDAI 78

HSP 2 Score: 41.5874 bits (96), Expect = 1.639e-24
Identity = 26/55 (47.27%), Postives = 31/55 (56.36%), Query Frame = 3 
Query:  279 GKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            G D   Q  IDN M++ LDGT N     K +LGAN  L VSLA  KA A  + +P
Sbjct:   80 GIDAENQIQIDNIMIE-LDGTPN-----KSRLGANAILGVSLAVAKAAAQASGLP 128
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENO_RHIEC (Enolase OS=Rhizobium etli (strain CFN 42 / ATCC 51251) GN=eno PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.639e-24
Identity = 49/75 (65.33%), Postives = 55/75 (73.33%), Query Frame = 2 
Query:   50 ITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPAL 274
            IT + AR+I DSR NPTVEVDV   DG + RAAVPSGASTG +EA+ELRDGG  YLGKGV KAV   N  I  A+
Sbjct:    4 ITDIIAREILDSRGNPTVEVDVYLEDGSMGRAAVPSGASTGAHEAVELRDGGKRYLGKGVEKAVEAANTEIFDAI 78

HSP 2 Score: 41.5874 bits (96), Expect = 1.639e-24
Identity = 26/55 (47.27%), Postives = 31/55 (56.36%), Query Frame = 3 
Query:  279 GKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            G D   Q  IDN M++ LDGT N     K +LGAN  L VSLA  KA A  + +P
Sbjct:   80 GIDAENQIQIDNIMIE-LDGTPN-----KSRLGANAILGVSLAVAKAAAQASGLP 128
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENO_CRYPA (Enolase OS=Cryphonectria parasitica GN=ENO1 PE=3 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 2.132e-24
Identity = 47/76 (61.84%), Postives = 57/76 (75.00%), Query Frame = 2 
Query:   50 ITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPAL 274
            I+ + AR ++DSR NPTVEVD+ T  G + RA VPSGASTG +EA ELRDG  S +LGKGV KAV NVN ++GP L
Sbjct:    3 ISKIHARYVYDSRGNPTVEVDLVTETG-LHRAIVPSGASTGQHEACELRDGDKSKWLGKGVLKAVENVNTVLGPEL 77

HSP 2 Score: 47.7506 bits (112), Expect = 2.132e-24
Identity = 29/59 (49.15%), Postives = 36/59 (61.02%), Query Frame = 3 
Query:  267 LHSAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            L  AG D  +QTA+D ++++ LDGT N     K KLGAN  L VSLA  KAGA    +P
Sbjct:   77 LIKAGLDVKDQTAVDEFLIK-LDGTAN-----KTKLGANAILGVSLAVAKAGAAEKGVP 129
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENOA_MACFA (Alpha-enolase OS=Macaca fascicularis GN=ENO1 PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 2.132e-24
Identity = 53/78 (67.95%), Postives = 62/78 (79.49%), Query Frame = 2 
Query:   44 ITITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPAL 274
            ++I  V AR+IFDSR NPTVEVD+ TS G + RAAVPSGASTGIYEALELRD   + Y+GKGVSKAV ++N  I PAL
Sbjct:    1 MSILKVHAREIFDSRGNPTVEVDLFTSKG-LFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPAL 77

HSP 2 Score: 39.6614 bits (91), Expect = 2.132e-24
Identity = 28/60 (46.67%), Postives = 33/60 (55.00%), Query Frame = 3 
Query:  264 ALHSAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            AL S   + TEQ  ID  M++ +DGT N     K K GAN  L VSLA  KAGA    +P
Sbjct:   76 ALVSKKLNVTEQEKIDKLMIE-MDGTEN-----KSKFGANAILGVSLAVCKAGAVEKGVP 129
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENO_AGRVS (Enolase OS=Agrobacterium vitis (strain S4 / ATCC BAA-846) GN=eno PE=3 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 2.133e-24
Identity = 50/75 (66.67%), Postives = 57/75 (76.00%), Query Frame = 2 
Query:   50 ITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPAL 274
            IT + AR+I DSR NPTVEVDV   DG + RAAVPSGASTG +EA+ELRDGG  YLGKGV KAV  VN+ I  A+
Sbjct:    4 ITDIIAREILDSRGNPTVEVDVYLEDGSMGRAAVPSGASTGAHEAVELRDGGKRYLGKGVEKAVEAVNSEIYDAI 78

HSP 2 Score: 39.2762 bits (90), Expect = 2.133e-24
Identity = 25/55 (45.45%), Postives = 30/55 (54.55%), Query Frame = 3 
Query:  279 GKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            G D   Q  ID  M ++LDGT N     K +LGAN  L VSLA  KA A  + +P
Sbjct:   80 GHDAENQIQIDTIM-RELDGTPN-----KSRLGANAILGVSLAVAKAAAEASGLP 128
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ERR3_YEAST (Enolase-related protein 3 OS=Saccharomyces cerevisiae GN=ERR3 PE=1 SV=2)

HSP 1 Score: 101.679 bits (252), Expect = 2.774e-24
Identity = 53/78 (67.95%), Postives = 68/78 (87.18%), Query Frame = 2 
Query:   44 ITITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPAL 274
            ++IT V AR ++DSR NPTVEV++TT +G + RA VPSGASTGI+EA+ELRDG  S+++GKGV+KAVSNVN+IIGPAL
Sbjct:    1 MSITKVHARTVYDSRGNPTVEVEITTENG-LFRAIVPSGASTGIHEAVELRDGNKSEWMGKGVTKAVSNVNSIIGPAL 77

HSP 2 Score: 32.3426 bits (72), Expect = 2.774e-24
Identity = 21/45 (46.67%), Postives = 24/45 (53.33%), Query Frame = 3 
Query:  291 TEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGA 425
            T Q  ID  M+  LDGT N     K +LGAN  L VSL   +A A
Sbjct:   85 TNQKGIDELMIS-LDGTSN-----KSRLGANAILGVSLCVARAAA 123
The following BLAST results are available for this feature:
BLAST of DY270620 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
ENO_METTP9.680e-2568.35Enolase OS=Methanosaeta thermophila (strain DSM 61... [more]
ENO_LACDB1.259e-2463.41Enolase OS=Lactobacillus delbrueckii subsp. bulgar... [more]
ENO_LACDA1.259e-2463.41Enolase OS=Lactobacillus delbrueckii subsp. bulgar... [more]
ENO_RHILW1.639e-2465.33Enolase OS=Rhizobium leguminosarum bv. trifolii (s... [more]
ENO_RHIL31.639e-2465.33Enolase OS=Rhizobium leguminosarum bv. viciae (str... [more]
ENO_RHIEC1.639e-2465.33Enolase OS=Rhizobium etli (strain CFN 42 / ATCC 51... [more]
ENO_CRYPA2.132e-2461.84Enolase OS=Cryphonectria parasitica GN=ENO1 PE=3 S... [more]
ENOA_MACFA2.132e-2467.95Alpha-enolase OS=Macaca fascicularis GN=ENO1 PE=2 ... [more]
ENO_AGRVS2.133e-2466.67Enolase OS=Agrobacterium vitis (strain S4 / ATCC B... [more]
ERR3_YEAST2.774e-2467.95Enolase-related protein 3 OS=Saccharomyces cerevis... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAP7YD04FM1 Fruit-TF Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY270620 ID=DY270620; Name=DY270620; organism=Citrus clementina; type=EST; length=1359bp
GTTCTAGATCTTTTTTCGGTTTTCCAGCTTTCAGAAAATGGCAATAACCA
TCACTGCCGTCAAAGCCAGGCAGATCTTTGACAGCCGTGTAAATCCAACG
GTGGAGGTCGACGTGACAACATCAGATGGCCACGTGGCTAGAGCTGCTGT
TCCAAGCGGTGCATCCACCGGAATTTATGAGGCGCTTGAACTAAGAGACG
GAGGCTCTGATTACCTAGGCAAGGGTGTATCGAAGGCTGTTAGCAATGTC
AACGCCATCATTGGCCCTGCACTCCGCTGGCAAGGACCCAACTGAGCAGA
CTGCTATTGACAACTATATGGTACAACAACTTGATGGAACTGTAAATGAG
TGGGGTTGGTGCAAACAGAAGCTTGGAGCCAACGGCCGTTTGGCTGTCTC
CCTAGCTACCCGCAAGGCCGGTGCACATGTCACGACCATTCCCCCTTTAC
CAGCTGATTGCCACACCTCCCCGGTAACAATAAGTTGGTAGTGGCCCGGG
ACCGACGTTCTATGTCCATTAATGGCGGACCTTACGCTGCGAACAAAGCT
TCCTTCCGACGGAATCTTTGTTTCCTATCCTACCGACTTACTCTATTTCT
ATATAAACCTTAATAATAGGGTAATTTATAACCGTACATATTAAGTAAGT
TCCACTTCTTCTAAGACACTACCCACCACCGGGACTAATGCTTGTAAAGG
GTAGGATCCCCCTGCACGTTCCCGACCACCAACCCCAACCAACATCAGAT
AGGGACCACTCAACTCTCCCATCTCAACCCACACCCAGAGACACCATCAC
ACCCGCTTTTACCCCCCCCAACACCCATGGCATGCACATCCCCCACAATC
TGTCTAGAGACAGCCAAGATCGTCTCAAGGCTGACCAAAGCATCTTTCGA
GACGAGCTGACTCTGATTTAAAATAATAACACTCCTCTATATATTACACT
CTTCCCCCAGCACCCTCTCACGACCTCCAGCGTCTGTAATTCACTTTTTC
AGATACCTAATGTTACAACTGTCTCAAGTTTTGCAAATGCCTCGCACATC
CTCGCCCCTGATATCTCGTAACTCTCTCCTTCCCATCCTTGCTTTTTCCC
TTCCTTATCTCCATTATCCAGTCTCTTACACTCTCTTATGCTCTACCATC
ATCCGCTCTCTATCCTTCCTCTACCGCCTACCGCACCCTGATGCAGCGAA
TACTACACCCACCTACCCACAGATTTCTCCCCCCTCTCCCCTCCTACAAC
AGTCCAAAACGCGATCCACACACTGTATACCGTAACTACTTAAATTCTCT
CGATATTTTTTAATCAGACCCCCTCTCATACATCAGCTCCTTGATCTTCT
TACTCTGCC
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