DY270620

Overview
NameDY270620
Unique NameDY270620
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length1359
Libraries
Library NameType
Fruit-TFcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ERR1_YEAST (Enolase-related protein 1/2 OS=Saccharomyces cerevisiae GN=ERR1 PE=1 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 2.774e-24
Identity = 53/78 (67.95%), Postives = 68/78 (87.18%), Query Frame = 2 
Query:   44 ITITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPAL 274
            ++IT V AR ++DSR NPTVEV++TT +G + RA VPSGASTGI+EA+ELRDG  S+++GKGV+KAVSNVN+IIGPAL
Sbjct:    1 MSITKVHARTVYDSRGNPTVEVEITTENG-LFRAIVPSGASTGIHEAVELRDGNKSEWMGKGVTKAVSNVNSIIGPAL 77

HSP 2 Score: 32.3426 bits (72), Expect = 2.774e-24
Identity = 21/45 (46.67%), Postives = 24/45 (53.33%), Query Frame = 3 
Query:  291 TEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGA 425
            T Q  ID  M+  LDGT N     K +LGAN  L VSL   +A A
Sbjct:   85 TNQKGIDELMIS-LDGTSN-----KSRLGANAILGVSLCVARAAA 123
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENO2_CHLTE (Enolase 2 OS=Chlorobium tepidum GN=eno2 PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 2.774e-24
Identity = 50/76 (65.79%), Postives = 57/76 (75.00%), Query Frame = 2 
Query:   50 ITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRD-GGSDYLGKGVSKAVSNVNAIIGPAL 274
            IT + ARQI DSR NPTVEVDV T +    RAAVPSGASTG++EA+ELRD   S +LGKGV KAV NVN +I  AL
Sbjct:    4 ITRIHARQIMDSRGNPTVEVDVHT-ESSFGRAAVPSGASTGVHEAVELRDKDKSVFLGKGVLKAVENVNTLINDAL 78

HSP 2 Score: 46.9802 bits (110), Expect = 2.774e-24
Identity = 29/55 (52.73%), Postives = 34/55 (61.82%), Query Frame = 3 
Query:  279 GKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            G D TEQ AID  +++ LDGT N     K KLGAN  L VSLA  KAGA  + +P
Sbjct:   80 GMDVTEQEAIDAKLIE-LDGTPN-----KSKLGANAILGVSLACAKAGAEYSALP 128
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENOA_PONAB (Alpha-enolase OS=Pongo abelii GN=ENO1 PE=2 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 2.775e-24
Identity = 52/78 (66.67%), Postives = 62/78 (79.49%), Query Frame = 2 
Query:   44 ITITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPAL 274
            ++I  + AR+IFDSR NPTVEVD+ TS G + RAAVPSGASTGIYEALELRD   + Y+GKGVSKAV ++N  I PAL
Sbjct:    1 MSILKIHAREIFDSRGNPTVEVDLFTSKG-LFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPAL 77

HSP 2 Score: 39.6614 bits (91), Expect = 2.775e-24
Identity = 28/60 (46.67%), Postives = 33/60 (55.00%), Query Frame = 3 
Query:  264 ALHSAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            AL S   + TEQ  ID  M++ +DGT N     K K GAN  L VSLA  KAGA    +P
Sbjct:   76 ALVSKKLNVTEQEKIDKLMIE-MDGTEN-----KSKFGANAILGVSLAVCKAGAVEKGVP 129
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENOA_HUMAN (Alpha-enolase OS=Homo sapiens GN=ENO1 PE=1 SV=2)

HSP 1 Score: 94.3597 bits (233), Expect = 2.775e-24
Identity = 52/78 (66.67%), Postives = 62/78 (79.49%), Query Frame = 2 
Query:   44 ITITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPAL 274
            ++I  + AR+IFDSR NPTVEVD+ TS G + RAAVPSGASTGIYEALELRD   + Y+GKGVSKAV ++N  I PAL
Sbjct:    1 MSILKIHAREIFDSRGNPTVEVDLFTSKG-LFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPAL 77

HSP 2 Score: 39.6614 bits (91), Expect = 2.775e-24
Identity = 28/60 (46.67%), Postives = 33/60 (55.00%), Query Frame = 3 
Query:  264 ALHSAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            AL S   + TEQ  ID  M++ +DGT N     K K GAN  L VSLA  KAGA    +P
Sbjct:   76 ALVSKKLNVTEQEKIDKLMIE-MDGTEN-----KSKFGANAILGVSLAVCKAGAVEKGVP 129
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENOG_MOUSE (Gamma-enolase OS=Mus musculus GN=Eno2 PE=1 SV=2)

HSP 1 Score: 91.2781 bits (225), Expect = 2.775e-24
Identity = 51/78 (65.38%), Postives = 61/78 (78.21%), Query Frame = 2 
Query:   44 ITITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSD-YLGKGVSKAVSNVNAIIGPAL 274
            ++I  + AR+I DSR NPTVEVD+ T+ G + RAAVPSGASTGIYEALELRDG    YLGKGV KAV ++N+ I PAL
Sbjct:    1 MSIEKIWAREILDSRGNPTVEVDLYTAKG-LFRAAVPSGASTGIYEALELRDGDKQRYLGKGVLKAVDHINSRIAPAL 77

HSP 2 Score: 42.743 bits (99), Expect = 2.775e-24
Identity = 29/60 (48.33%), Postives = 34/60 (56.67%), Query Frame = 3 
Query:  264 ALHSAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            AL S+G    EQ  +DN M++ LDGT N     K K GAN  L VSLA  KAGA    +P
Sbjct:   76 ALISSGISVVEQEKLDNLMLE-LDGTEN-----KSKFGANAILGVSLAVCKAGAAERDLP 129
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENO_CLOTH (Enolase OS=Clostridium thermocellum (strain ATCC 27405 / DSM 1237) GN=eno PE=3 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 3.611e-24
Identity = 50/79 (63.29%), Postives = 57/79 (72.15%), Query Frame = 2 
Query:   35 KMAITITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSD-YLGKGVSKAVSNVNAIIGP 268
            K  + I +V AR+I DSR NPTVEV+V    G V RAAVPSGASTG +EA+ELRD   + YLGKGV KAV NVN II P
Sbjct:    2 KQYLEIESVFAREILDSRGNPTVEVEVIAEGGFVGRAAVPSGASTGAFEAVELRDNDKNRYLGKGVQKAVENVNNIIAP 80

HSP 2 Score: 41.5874 bits (96), Expect = 3.611e-24
Identity = 26/49 (53.06%), Postives = 30/49 (61.22%), Query Frame = 3 
Query:  279 GKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGA 425
            G +  +Q A+DN M+  LDGT N     K KLGAN  L VSLAT KA A
Sbjct:   84 GMNVFDQAAVDNLMIS-LDGTPN-----KSKLGANAILGVSLATAKAAA 126
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENO_BACP2 (Enolase OS=Bacillus pumilus (strain SAFR-032) GN=eno PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.611e-24
Identity = 50/76 (65.79%), Postives = 55/76 (72.37%), Query Frame = 2 
Query:   50 ITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSD-YLGKGVSKAVSNVNAIIGPAL 274
            I  V AR++ DSR NPTVEV+V T  G   RA VPSGASTG YEA+ELRDG  D YLGKGV  AV+NVN II P L
Sbjct:    4 IVDVYAREVLDSRGNPTVEVEVYTESGGFGRALVPSGASTGEYEAVELRDGDKDRYLGKGVLTAVNNVNEIIAPEL 79

HSP 2 Score: 42.3578 bits (98), Expect = 3.611e-24
Identity = 27/55 (49.09%), Postives = 32/55 (58.18%), Query Frame = 3 
Query:  279 GKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            G D TEQ AID  ++ +LDGT N     K KLGAN  L VS+A  +A A    IP
Sbjct:   81 GFDVTEQVAIDKMLI-ELDGTEN-----KGKLGANAILGVSIAVARAAADFLQIP 129
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENO_KLULA (Enolase OS=Kluyveromyces lactis GN=ENO PE=3 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 4.698e-24
Identity = 50/76 (65.79%), Postives = 59/76 (77.63%), Query Frame = 2 
Query:   50 ITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPAL 274
            I+ V AR ++DSR NPTVEV++TT  G   R+ VPSGASTG++EALELRDG  S +LGKGV  AVSNVN II PAL
Sbjct:    3 ISKVYARSVYDSRGNPTVEVELTTEKG-TFRSIVPSGASTGVHEALELRDGDKSKWLGKGVLTAVSNVNNIIAPAL 77

HSP 2 Score: 43.8986 bits (102), Expect = 4.698e-24
Identity = 28/60 (46.67%), Postives = 33/60 (55.00%), Query Frame = 3 
Query:  264 ALHSAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            AL  A  D T Q  +D+ ++  LDGT N     K KLGAN  L VSLA  KAGA    +P
Sbjct:   76 ALVKANVDVTNQQQVDDLLLS-LDGTAN-----KSKLGANAILGVSLAASKAGAAAKNVP 129
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENO_CAEEL (Enolase OS=Caenorhabditis elegans GN=enol-1 PE=1 SV=3)

HSP 1 Score: 94.7449 bits (234), Expect = 4.699e-24
Identity = 54/80 (67.50%), Postives = 63/80 (78.75%), Query Frame = 2 
Query:   50 ITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPALRWQG 286
            IT + ARQI+DSR NPTVEVD+ T  G V RAAVPSGASTG++EALELRDG  + +LGKGV KAVSN+N  I PAL  +G
Sbjct:    3 ITKIHARQIYDSRGNPTVEVDLFTEKG-VFRAAVPSGASTGVHEALELRDGDKAVHLGKGVLKAVSNINEKIAPALIAKG 81

HSP 2 Score: 38.5058 bits (88), Expect = 4.699e-24
Identity = 27/54 (50.00%), Postives = 32/54 (59.26%), Query Frame = 3 
Query:  264 ALHSAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGA 425
            AL + G D T Q  ID++M+  LDG+ N     K  LGAN  L VSLA  KAGA
Sbjct:   76 ALIAKGFDVTAQKDIDDFMMA-LDGSEN-----KGNLGANAILGVSLAVAKAGA 123
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENO_DESAD (Enolase OS=Desulfovibrio salexigens (strain ATCC 14822 / DSM 2638 / NCIB 8403 / VKM B-1763) GN=eno PE=3 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 4.699e-24
Identity = 51/77 (66.23%), Postives = 54/77 (70.13%), Query Frame = 2 
Query:   47 TITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSD-YLGKGVSKAVSNVNAIIGPAL 274
            TITAV AR+I DSR NPTVEV+V    G   RAAVPSGASTG  EALELRDG  D Y GKGV  AV+NV   I  AL
Sbjct:    3 TITAVWAREILDSRGNPTVEVEVVLESGATGRAAVPSGASTGTREALELRDGDKDRYKGKGVQVAVANVREEIAEAL 79

HSP 2 Score: 44.669 bits (104), Expect = 4.699e-24
Identity = 25/55 (45.45%), Postives = 35/55 (63.64%), Query Frame = 3 
Query:  279 GKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            G+D   Q  IDN +++ LDGT N     K++LGAN  L VS+A  +AGA++  IP
Sbjct:   81 GQDALRQVNIDNILIE-LDGTEN-----KERLGANAMLGVSMAVARAGANLLGIP 129
The following BLAST results are available for this feature:
BLAST of DY270620 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
ERR1_YEAST2.774e-2467.95Enolase-related protein 1/2 OS=Saccharomyces cerev... [more]
ENO2_CHLTE2.774e-2465.79Enolase 2 OS=Chlorobium tepidum GN=eno2 PE=3 SV=1[more]
ENOA_PONAB2.775e-2466.67Alpha-enolase OS=Pongo abelii GN=ENO1 PE=2 SV=1[more]
ENOA_HUMAN2.775e-2466.67Alpha-enolase OS=Homo sapiens GN=ENO1 PE=1 SV=2[more]
ENOG_MOUSE2.775e-2465.38Gamma-enolase OS=Mus musculus GN=Eno2 PE=1 SV=2[more]
ENO_CLOTH3.611e-2463.29Enolase OS=Clostridium thermocellum (strain ATCC 2... [more]
ENO_BACP23.611e-2465.79Enolase OS=Bacillus pumilus (strain SAFR-032) GN=e... [more]
ENO_KLULA4.698e-2465.79Enolase OS=Kluyveromyces lactis GN=ENO PE=3 SV=1[more]
ENO_CAEEL4.699e-2467.50Enolase OS=Caenorhabditis elegans GN=enol-1 PE=1 S... [more]
ENO_DESAD4.699e-2466.23Enolase OS=Desulfovibrio salexigens (strain ATCC 1... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAP7YD04FM1 Fruit-TF Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY270620 ID=DY270620; Name=DY270620; organism=Citrus clementina; type=EST; length=1359bp
GTTCTAGATCTTTTTTCGGTTTTCCAGCTTTCAGAAAATGGCAATAACCA
TCACTGCCGTCAAAGCCAGGCAGATCTTTGACAGCCGTGTAAATCCAACG
GTGGAGGTCGACGTGACAACATCAGATGGCCACGTGGCTAGAGCTGCTGT
TCCAAGCGGTGCATCCACCGGAATTTATGAGGCGCTTGAACTAAGAGACG
GAGGCTCTGATTACCTAGGCAAGGGTGTATCGAAGGCTGTTAGCAATGTC
AACGCCATCATTGGCCCTGCACTCCGCTGGCAAGGACCCAACTGAGCAGA
CTGCTATTGACAACTATATGGTACAACAACTTGATGGAACTGTAAATGAG
TGGGGTTGGTGCAAACAGAAGCTTGGAGCCAACGGCCGTTTGGCTGTCTC
CCTAGCTACCCGCAAGGCCGGTGCACATGTCACGACCATTCCCCCTTTAC
CAGCTGATTGCCACACCTCCCCGGTAACAATAAGTTGGTAGTGGCCCGGG
ACCGACGTTCTATGTCCATTAATGGCGGACCTTACGCTGCGAACAAAGCT
TCCTTCCGACGGAATCTTTGTTTCCTATCCTACCGACTTACTCTATTTCT
ATATAAACCTTAATAATAGGGTAATTTATAACCGTACATATTAAGTAAGT
TCCACTTCTTCTAAGACACTACCCACCACCGGGACTAATGCTTGTAAAGG
GTAGGATCCCCCTGCACGTTCCCGACCACCAACCCCAACCAACATCAGAT
AGGGACCACTCAACTCTCCCATCTCAACCCACACCCAGAGACACCATCAC
ACCCGCTTTTACCCCCCCCAACACCCATGGCATGCACATCCCCCACAATC
TGTCTAGAGACAGCCAAGATCGTCTCAAGGCTGACCAAAGCATCTTTCGA
GACGAGCTGACTCTGATTTAAAATAATAACACTCCTCTATATATTACACT
CTTCCCCCAGCACCCTCTCACGACCTCCAGCGTCTGTAATTCACTTTTTC
AGATACCTAATGTTACAACTGTCTCAAGTTTTGCAAATGCCTCGCACATC
CTCGCCCCTGATATCTCGTAACTCTCTCCTTCCCATCCTTGCTTTTTCCC
TTCCTTATCTCCATTATCCAGTCTCTTACACTCTCTTATGCTCTACCATC
ATCCGCTCTCTATCCTTCCTCTACCGCCTACCGCACCCTGATGCAGCGAA
TACTACACCCACCTACCCACAGATTTCTCCCCCCTCTCCCCTCCTACAAC
AGTCCAAAACGCGATCCACACACTGTATACCGTAACTACTTAAATTCTCT
CGATATTTTTTAATCAGACCCCCTCTCATACATCAGCTCCTTGATCTTCT
TACTCTGCC
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