DY270620

Overview
NameDY270620
Unique NameDY270620
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length1359
Libraries
Library NameType
Fruit-TFcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENO_CLOBK (Enolase OS=Clostridium botulinum (strain Okra / Type B1) GN=eno PE=3 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.036e-23
Identity = 54/81 (66.67%), Postives = 57/81 (70.37%), Query Frame = 2 
Query:   35 KMAITITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPAL 274
            K  I I  V ARQI DSR NPTVEV+V   DG V  AAVPSGASTG +EA+ELRDG  S YLGKGV KAV NVN II   L
Sbjct:    2 KNYIEIVDVYARQILDSRCNPTVEVEVELEDGTVGVAAVPSGASTGAFEAVELRDGDKSKYLGKGVLKAVDNVNTIIADEL 82

HSP 2 Score: 38.5058 bits (88), Expect = 1.036e-23
Identity = 25/50 (50.00%), Postives = 30/50 (60.00%), Query Frame = 3 
Query:  279 GKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAH 428
            G +  +Q AID  M++ LDGT N     K KLGAN  L VSLA  KA A+
Sbjct:   84 GMNVLDQVAIDKTMIE-LDGTDN-----KAKLGANAMLGVSLACAKAAAN 127
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENO_CLOB6 (Enolase OS=Clostridium botulinum (strain 657 / Type Ba4) GN=eno PE=3 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.036e-23
Identity = 54/81 (66.67%), Postives = 57/81 (70.37%), Query Frame = 2 
Query:   35 KMAITITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPAL 274
            K  I I  V ARQI DSR NPTVEV+V   DG V  AAVPSGASTG +EA+ELRDG  S YLGKGV KAV NVN II   L
Sbjct:    2 KNYIEIVDVYARQILDSRCNPTVEVEVELEDGTVGVAAVPSGASTGAFEAVELRDGDKSKYLGKGVLKAVDNVNTIIADEL 82

HSP 2 Score: 38.5058 bits (88), Expect = 1.036e-23
Identity = 25/50 (50.00%), Postives = 30/50 (60.00%), Query Frame = 3 
Query:  279 GKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAH 428
            G +  +Q AID  M++ LDGT N     K KLGAN  L VSLA  KA A+
Sbjct:   84 GMNVLDQVAIDKTMIE-LDGTDN-----KAKLGANAMLGVSLACAKAAAN 127
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENO_CLOB1 (Enolase OS=Clostridium botulinum (strain ATCC 19397 / Type A) GN=eno PE=3 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.036e-23
Identity = 54/81 (66.67%), Postives = 57/81 (70.37%), Query Frame = 2 
Query:   35 KMAITITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPAL 274
            K  I I  V ARQI DSR NPTVEV+V   DG V  AAVPSGASTG +EA+ELRDG  S YLGKGV KAV NVN II   L
Sbjct:    2 KNYIEIVDVYARQILDSRCNPTVEVEVELEDGTVGVAAVPSGASTGAFEAVELRDGDKSKYLGKGVLKAVDNVNTIIADEL 82

HSP 2 Score: 38.5058 bits (88), Expect = 1.036e-23
Identity = 25/50 (50.00%), Postives = 30/50 (60.00%), Query Frame = 3 
Query:  279 GKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAH 428
            G +  +Q AID  M++ LDGT N     K KLGAN  L VSLA  KA A+
Sbjct:   84 GMNVLDQVAIDKTMIE-LDGTDN-----KAKLGANAMLGVSLACAKAAAN 127
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENO_DEIDV (Enolase OS=Deinococcus deserti (strain VCD115 / DSM 17065 / LMG 22923) GN=eno PE=3 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 1.036e-23
Identity = 47/77 (61.04%), Postives = 56/77 (72.73%), Query Frame = 2 
Query:   44 ITITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPAL 274
            + I  V AR++ DSR NPTVE +V    G V RA VPSGASTG +EALELRDGGS Y+GKGV +AV NV   +GPA+
Sbjct:    1 MNIEKVIAREVLDSRGNPTVEAEVHLDSGFVGRAIVPSGASTGTHEALELRDGGSRYMGKGVQQAVRNVEEALGPAI 77

HSP 2 Score: 38.1206 bits (87), Expect = 1.036e-23
Identity = 25/55 (45.45%), Postives = 32/55 (58.18%), Query Frame = 3 
Query:  279 GKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            G D +EQ AID  ++  +DGT N     K K+G N  LAVSLAT +A A    +P
Sbjct:   79 GLDASEQIAIDAALMA-VDGTPN-----KGKMGGNAILAVSLATSRAAAEELGVP 127
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENOB_HUMAN (Beta-enolase OS=Homo sapiens GN=ENO3 PE=1 SV=4)

HSP 1 Score: 93.5893 bits (231), Expect = 1.347e-23
Identity = 51/71 (71.83%), Postives = 57/71 (80.28%), Query Frame = 2 
Query:   65 ARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSD-YLGKGVSKAVSNVNAIIGPAL 274
            AR+I DSR NPTVEVD+ T+ G   RAAVPSGASTGIYEALELRDG    YLGKGV KAV N+N+ +GPAL
Sbjct:    8 AREILDSRGNPTVEVDLHTAKGRF-RAAVPSGASTGIYEALELRDGDKGRYLGKGVLKAVENINSTLGPAL 77

HSP 2 Score: 38.1206 bits (87), Expect = 1.347e-23
Identity = 27/65 (41.54%), Postives = 33/65 (50.77%), Query Frame = 3 
Query:  249 STPSLALHSAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            ST   AL        +Q  +D +M++ LDGT N     K K GAN  L VSLA  KAGA    +P
Sbjct:   71 STLGPALLQKKLSVADQEKVDKFMIE-LDGTEN-----KSKFGANAILGVSLAVCKAGAAEKGVP 129
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENOA_DICDI (Enolase A OS=Dictyostelium discoideum GN=enoA PE=3 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.347e-23
Identity = 51/79 (64.56%), Postives = 62/79 (78.48%), Query Frame = 2 
Query:   50 ITAVKARQIFDSRVNPTVEVDV-TTSDGHVA--RAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPAL 274
            I ++KAR+I +SR NPTVEVD+ T  DG V+  RAAVPSGASTGIYEA+ELRDG  S YLGKGV KA+ N+  +I PA+
Sbjct:    4 IKSIKAREILNSRGNPTVEVDLYTEKDGVVSSFRAAVPSGASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAV 82

HSP 2 Score: 43.1282 bits (100), Expect = 1.347e-23
Identity = 28/55 (50.91%), Postives = 33/55 (60.00%), Query Frame = 3 
Query:  279 GKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            GK  ++Q AID  M+  LDGT N     K KLGAN  LAVSLA  +AGA    +P
Sbjct:   84 GKSVSDQAAIDKLMID-LDGTPN-----KGKLGANAILAVSLAVCRAGAADRNLP 132
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENO_TUBBO (Enolase OS=Tuber borchii GN=eno-1 PE=1 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.753e-23
Identity = 48/76 (63.16%), Postives = 58/76 (76.32%), Query Frame = 2 
Query:   50 ITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRD-GGSDYLGKGVSKAVSNVNAIIGPAL 274
            IT + AR ++DSR NPTVEVD+TT+D  + RA VPSGASTG +EA+ELRD   + + GKGV KAV NVN II PAL
Sbjct:    2 ITKIHARSVYDSRGNPTVEVDLTTTDTGLHRAIVPSGASTGQHEAIELRDKDKTKWAGKGVLKAVENVNTIIAPAL 77

HSP 2 Score: 41.2022 bits (95), Expect = 1.753e-23
Identity = 25/53 (47.17%), Postives = 30/53 (56.60%), Query Frame = 3 
Query:  285 DPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            D  +Q  ID +++  LDGT N     K KLGAN  L VSLA  KAGA    +P
Sbjct:   83 DVKDQATIDKFLID-LDGTPN-----KAKLGANAILGVSLAVAKAGAAAKKVP 129
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENOA_BOVIN (Alpha-enolase OS=Bos taurus GN=ENO1 PE=1 SV=4)

HSP 1 Score: 93.5893 bits (231), Expect = 1.753e-23
Identity = 52/78 (66.67%), Postives = 62/78 (79.49%), Query Frame = 2 
Query:   44 ITITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPAL 274
            ++I  V AR+IFDSR NPTVEVD+ T+ G + RAAVPSGASTGIYEALELRD   + Y+GKGVSKAV ++N  I PAL
Sbjct:    1 MSILKVHAREIFDSRGNPTVEVDLFTAKG-LFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPAL 77

HSP 2 Score: 37.7354 bits (86), Expect = 1.753e-23
Identity = 27/60 (45.00%), Postives = 32/60 (53.33%), Query Frame = 3 
Query:  264 ALHSAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            AL S   +  EQ  ID  M++ +DGT N     K K GAN  L VSLA  KAGA    +P
Sbjct:   76 ALVSKKLNVVEQEKIDKLMIE-MDGTEN-----KSKFGANAILGVSLAVCKAGAVEKGVP 129
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENO_AGRRK (Enolase OS=Agrobacterium radiobacter (strain K84 / ATCC BAA-868) GN=eno PE=3 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 1.754e-23
Identity = 49/75 (65.33%), Postives = 55/75 (73.33%), Query Frame = 2 
Query:   50 ITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPAL 274
            IT + AR+I DSR NPTVEVDV   DG + RAAVPSGASTG +EA+ELRDGG  Y GKGV KAV  VN  I  A+
Sbjct:    4 ITDIIAREILDSRGNPTVEVDVYLEDGSLGRAAVPSGASTGAHEAVELRDGGKRYHGKGVEKAVEAVNTEIFDAI 78

HSP 2 Score: 39.2762 bits (90), Expect = 1.754e-23
Identity = 24/55 (43.64%), Postives = 30/55 (54.55%), Query Frame = 3 
Query:  279 GKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAHVTTIP 443
            G D   Q  ID+ M++ LDGT N     K ++GAN  L VSLA  KA A    +P
Sbjct:   80 GMDAENQIQIDDIMIE-LDGTPN-----KSRIGANAILGVSLAVAKAAAQSANLP 128
[Close]
BLAST of DY270620 vs. ExPASy Swiss-Prot
Match: ENO_CLOBJ (Enolase OS=Clostridium botulinum (strain Kyoto / Type A2) GN=eno PE=3 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 2.282e-23
Identity = 53/81 (65.43%), Postives = 56/81 (69.14%), Query Frame = 2 
Query:   35 KMAITITAVKARQIFDSRVNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPAL 274
            K  I I  V ARQI DSR NPTVEV+V   DG V  AAVPSGASTG +EA+ELRDG  S YLGKGV KAV NVN  I   L
Sbjct:    2 KNYIEIVDVYARQILDSRCNPTVEVEVELEDGTVGVAAVPSGASTGAFEAVELRDGDKSQYLGKGVLKAVDNVNTTIADEL 82

HSP 2 Score: 38.891 bits (89), Expect = 2.282e-23
Identity = 26/57 (45.61%), Postives = 33/57 (57.89%), Query Frame = 3 
Query:  258 SLALHSAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANGRLAVSLATRKAGAH 428
            ++A    G +  +Q AID  M++ LDGT N     K KLGAN  L VSLA  KA A+
Sbjct:   77 TIADELVGMNVLDQVAIDKTMIE-LDGTDN-----KAKLGANAMLGVSLACAKAAAN 127
The following BLAST results are available for this feature:
BLAST of DY270620 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
ENO_CLOBK1.036e-2366.67Enolase OS=Clostridium botulinum (strain Okra / Ty... [more]
ENO_CLOB61.036e-2366.67Enolase OS=Clostridium botulinum (strain 657 / Typ... [more]
ENO_CLOB11.036e-2366.67Enolase OS=Clostridium botulinum (strain ATCC 1939... [more]
ENO_DEIDV1.036e-2361.04Enolase OS=Deinococcus deserti (strain VCD115 / DS... [more]
ENOB_HUMAN1.347e-2371.83Beta-enolase OS=Homo sapiens GN=ENO3 PE=1 SV=4[more]
ENOA_DICDI1.347e-2364.56Enolase A OS=Dictyostelium discoideum GN=enoA PE=3... [more]
ENO_TUBBO1.753e-2363.16Enolase OS=Tuber borchii GN=eno-1 PE=1 SV=1[more]
ENOA_BOVIN1.753e-2366.67Alpha-enolase OS=Bos taurus GN=ENO1 PE=1 SV=4[more]
ENO_AGRRK1.754e-2365.33Enolase OS=Agrobacterium radiobacter (strain K84 /... [more]
ENO_CLOBJ2.282e-2365.43Enolase OS=Clostridium botulinum (strain Kyoto / T... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAP7YD04FM1 Fruit-TF Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY270620 ID=DY270620; Name=DY270620; organism=Citrus clementina; type=EST; length=1359bp
GTTCTAGATCTTTTTTCGGTTTTCCAGCTTTCAGAAAATGGCAATAACCA
TCACTGCCGTCAAAGCCAGGCAGATCTTTGACAGCCGTGTAAATCCAACG
GTGGAGGTCGACGTGACAACATCAGATGGCCACGTGGCTAGAGCTGCTGT
TCCAAGCGGTGCATCCACCGGAATTTATGAGGCGCTTGAACTAAGAGACG
GAGGCTCTGATTACCTAGGCAAGGGTGTATCGAAGGCTGTTAGCAATGTC
AACGCCATCATTGGCCCTGCACTCCGCTGGCAAGGACCCAACTGAGCAGA
CTGCTATTGACAACTATATGGTACAACAACTTGATGGAACTGTAAATGAG
TGGGGTTGGTGCAAACAGAAGCTTGGAGCCAACGGCCGTTTGGCTGTCTC
CCTAGCTACCCGCAAGGCCGGTGCACATGTCACGACCATTCCCCCTTTAC
CAGCTGATTGCCACACCTCCCCGGTAACAATAAGTTGGTAGTGGCCCGGG
ACCGACGTTCTATGTCCATTAATGGCGGACCTTACGCTGCGAACAAAGCT
TCCTTCCGACGGAATCTTTGTTTCCTATCCTACCGACTTACTCTATTTCT
ATATAAACCTTAATAATAGGGTAATTTATAACCGTACATATTAAGTAAGT
TCCACTTCTTCTAAGACACTACCCACCACCGGGACTAATGCTTGTAAAGG
GTAGGATCCCCCTGCACGTTCCCGACCACCAACCCCAACCAACATCAGAT
AGGGACCACTCAACTCTCCCATCTCAACCCACACCCAGAGACACCATCAC
ACCCGCTTTTACCCCCCCCAACACCCATGGCATGCACATCCCCCACAATC
TGTCTAGAGACAGCCAAGATCGTCTCAAGGCTGACCAAAGCATCTTTCGA
GACGAGCTGACTCTGATTTAAAATAATAACACTCCTCTATATATTACACT
CTTCCCCCAGCACCCTCTCACGACCTCCAGCGTCTGTAATTCACTTTTTC
AGATACCTAATGTTACAACTGTCTCAAGTTTTGCAAATGCCTCGCACATC
CTCGCCCCTGATATCTCGTAACTCTCTCCTTCCCATCCTTGCTTTTTCCC
TTCCTTATCTCCATTATCCAGTCTCTTACACTCTCTTATGCTCTACCATC
ATCCGCTCTCTATCCTTCCTCTACCGCCTACCGCACCCTGATGCAGCGAA
TACTACACCCACCTACCCACAGATTTCTCCCCCCTCTCCCCTCCTACAAC
AGTCCAAAACGCGATCCACACACTGTATACCGTAACTACTTAAATTCTCT
CGATATTTTTTAATCAGACCCCCTCTCATACATCAGCTCCTTGATCTTCT
TACTCTGCC
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