FC868653

Overview
NameFC868653
Unique NameFC868653
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length713
Libraries
Library NameType
AbioticL1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC868653 vs. ExPASy Swiss-Prot
Match: GLGA_METJA (Probable glycogen synthase OS=Methanocaldococcus jannaschii GN=glgA PE=3 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 3.731e-22
Identity = 73/252 (28.97%), Postives = 124/252 (49.21%), Query Frame = 2
Query:   23 INWMKAGILESDMVLTVSPHYAQELVSGEDKGVELDNIIRKTGIKGIVNGMDVQEWNPL-----------------TDKYIGVKYDASTVMDAKPLLK-----------------------EALQAEVGLPVDRNI--PVIGFIGRLEEQKGSDILAAAIPHFI-KENVQIIVLGTGKKPMEKQLEQLEILYPEKARGVAKFNIPLAHMIIAGADFILIPSRFEPCGLIQLHAMRYGTVPIVASTGGLVDTV 649
            + +++ GI  +D V TVSP +A+E+ +       +   +      GI+NG+D+ E++P+                    YI   Y A    + KP +K                        A   EV   VD +I  P+IGF+GR   QKG + +  AIP  + K +++ + L  G + +E++L+ L   +  +   +  +++PL+ ++ AG+D+I++PS +EPCGL+Q+ AM Y T  I   TGGL DT+
Sbjct:  165 MTFLELGIKYADAVNTVSPSHAEEIKNYP----YIKKYLNNKPFCGILNGIDIDEYDPMKIIERMCNLSNNKLDPRNYAYIS-PYSAEDSHNIKPKIKYSWFYRGGVYEYVEDWNKIDKGISATDVEVHGGVDGDIETPLIGFVGRATHQKGFNTMFEAIPELLEKHDIRFVFLTKGDRDIEERLKNLANEHDGRILALIGYSLPLSSLVFAGSDWIIMPSYWEPCGLVQMEAMAYCTPVIATETGGLKDTI 411          
BLAST of FC868653 vs. ExPASy Swiss-Prot
Match: MOK12_SCHPO (Cell wall alpha-1,3-glucan synthase mok12 OS=Schizosaccharomyces pombe GN=mok12 PE=2 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 2.424e-13
Identity = 59/161 (36.65%), Postives = 85/161 (52.80%), Query Frame = 2
Query:  176 DVQEWNPLTDKYIGVKYDASTVMDAK-PLLKEALQAEVGLPVDRNIPVIGFIGRLEEQKGSDILAAAIPHFIKE-NVQIIVLGT-----GKKPMEKQLEQLEILYPEKARGVAKFNIPLAHMIIAGADFILIPSRFEPCGLIQLHAMRYGTVPIVASTGGL 637
            D++  N LTD  + V +    V++AK  LLK+  Q    L VD +  ++ F+GR   QKG D++A   P  + E NVQ+I +G      G+   EK LEQ+   +P +      F   +   + AG DF LIPSR EP GL+ +   R G + I + TGGL
Sbjct: 1423 DLKLLNHLTDDPVDVDF----VLEAKRKLLKKQTQEWANLDVDPSAQLLVFVGRWSHQKGIDLIADLAPKLLTEHNVQLITIGPVIDLHGQFAAEK-LEQIAKRFPTRVLCKPVFTA-VPPFLFAGTDFALIPSRDEPFGLVAVEFGRKGVLCIGSRTGGL 1577          
BLAST of FC868653 vs. ExPASy Swiss-Prot
Match: AGS1_SCHPO (Cell wall alpha-1,3-glucan synthase ags1 OS=Schizosaccharomyces pombe GN=ags1 PE=1 SV=3)

HSP 1 Score: 70.4774 bits (171), Expect = 1.018e-11
Identity = 57/157 (36.31%), Postives = 78/157 (49.68%), Query Frame = 2
Query:  200 TDKYIGVKYDASTVMD-----AKPLLKEALQAEVGLPVDRNIPVIGFIGRLEEQKGSDILAAAIPHFIKE-NVQIIVLGT-----GKKPMEKQLEQLEILYPEKARGVAKFNIPLAHMIIAGADFILIPSRFEPCGLIQLHAMRYGTVPIVASTGGL 637
            TD+ +  K  A T +D     +K   K   Q   GL V+    ++ F+GR   QKG D++A   P  ++   VQ+I +G      GK   EK L+ L+  YP +     KF   L   I +GADF LIPSR EP GL+ +   R G + I A  GGL
Sbjct: 1396 TDEIVDDKAVAITDIDPDMEKSKVEHKRLAQEWAGLEVNEKYDLLVFVGRWSSQKGIDLIADIAPSLLESYKVQLICVGPIIDLYGKFAAEK-LDVLQKKYPTRVFSQPKFT-QLPPYIFSGADFALIPSRDEPFGLVAVEFGRKGALGIGARVGGL 1550          
BLAST of FC868653 vs. ExPASy Swiss-Prot
Match: MOK14_SCHPO (Cell wall alpha-1,3-glucan synthase mok14 OS=Schizosaccharomyces pombe GN=mok14 PE=1 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.330e-11
Identity = 50/131 (38.17%), Postives = 69/131 (52.67%), Query Frame = 2
Query:  263 KEALQAEVGLPVDRNIPVIGFIGRLEEQKGSDILAAAIPHFIKE-NVQIIVLGT-----GKKPMEKQLEQLEILYPEKARGVAKFNIPLAHMIIAGADFILIPSRFEPCGLIQLHAMRYGTVPIVASTGGL 637
            K   Q   GL +D    ++ F+GR   QKG DILA   P  +++ N+Q+IV+G      GK   EK +  +E  YP +     +F + L   I  GADF LIPSR EP GL+ +   R G + I +  GGL
Sbjct:  532 KRKAQEWAGLTIDDEADLLVFVGRWSVQKGIDILADLAPTLLEKFNIQLIVVGPLIDLYGKFAAEKFMYIME-RYPGRVFSKPEF-VHLPPFIFEGADFALIPSRDEPFGLVAVEFGRRGAICIGSRVGGL 660          
The following BLAST results are available for this feature:
BLAST of FC868653 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 364
Match NameE-valueIdentityDescription
GLGA_METJA3.731e-2228.97Probable glycogen synthase OS=Methanocaldococcus j... [more]
MOK12_SCHPO2.424e-1336.65Cell wall alpha-1,3-glucan synthase mok12 OS=Schiz... [more]
AGS1_SCHPO1.018e-1136.31Cell wall alpha-1,3-glucan synthase ags1 OS=Schizo... [more]
MOK14_SCHPO1.330e-1138.17Cell wall alpha-1,3-glucan synthase mok14 OS=Schiz... [more]

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Properties
Property NameValue
Genbank descriptionC31003B03EF AbioticL1 Citrus clementina cDNA clone C31003B03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC868653 ID=FC868653; Name=FC868653; organism=Citrus clementina; type=EST; length=713bp
ATAAACCTGTGAGAGGATCAAAATCAACTGGATGAAAGCTGGAATATTAG
AATCAGACATGGTCTTAACTGTGAGCCCACACTATGCCCAAGAACTTGTT
TCTGGTGAAGACAAAGGAGTAGAATTGGATAACATCATCCGTAAAACTGG
CATTAAGGGAATTGTGAACGGGATGGATGTCCAGGAGTGGAACCCCTTGA
CTGACAAATATATAGGTGTCAAATATGATGCTTCAACTGTGATGGATGCG
AAGCCTCTTCTGAAGGAAGCCCTCCAAGCAGAAGTTGGGTTGCCTGTCGA
TAGAAATATTCCTGTCATAGGCTTCATTGGTAGACTTGAAGAGCAGAAAG
GCTCAGATATTCTTGCAGCTGCCATTCCCCATTTCATCAAAGAGAATGTT
CAGATAATAGTCCTTGGTACTGGCAAAAAACCAATGGAGAAGCAGCTTGA
ACAGTTGGAGATACTGTACCCTGAGAAAGCCAGAGGAGTAGCGAAATTCA
ATATTCCTCTGGCGCATATGATAATAGCAGGAGCTGATTTTATTTTGATT
CCAAGCAGATTTGAACCTTGTGGTCTCATTCAATTACATGCCATGCGTTA
TGGAACCGTACCTATCGTGGCTTCCACTGGTGGTTTGGTTGACACTGTGG
CAGAAGGCTTTACAGGATTCCAGATGGGAAGCTTCAGTGTTGACTGTGAG
GCTGTGGATCCAG
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