FC868749 | Citrus Genome Database

Overview


Name	FC868749
Unique Name	FC868749
Type	EST
Organism	Citrus clementina (Clementine)
Sequence length	707

Libraries


Library Name	Type
AbioticL1	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Ccv1_Contig10338	contig	Ccv1_Contig10338:2..706.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of FC868749 vs. ExPASy Swiss-Prot
Match: UBIQ_LEIMA (Ubiquitin OS=Leishmania major GN=UB-EP52 PE=3 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 2.100e-33
Identity = 71/76 (93.42%), Postives = 74/76 (97.37%), Query Frame = 1

Query:   70 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 297
            MQIFVKTLTGKTI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 142.51 bits (358), Expect = 2.100e-33
Identity = 71/76 (93.42%), Postives = 74/76 (97.37%), Query Frame = 1

Query:  298 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 525
            MQIFVKTLTGKTI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 3 Score: 88.5817 bits (218), Expect = 4.753e-18
Identity = 44/48 (91.67%), Postives = 45/48 (93.75%), Query Frame = 1

Query:  526 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQXRLIFAGK 669
            MQIFVKTLTGKTI LEVE SDTI+NVKAKIQDKEGIPPDQ RLIFAGK
Sbjct:    1 MQIFVKTLTGKTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGK 48

HSP 4 Score: 21.9422 bits (45), Expect = 4.753e-18
Identity = 8/10 (80.00%), Postives = 10/10 (100.00%), Query Frame = 3

Query:  672 LEDGRTLADY 701
            LE+GRTL+DY
Sbjct:   50 LEEGRTLSDY 59

[Close]

BLAST of FC868749 vs. ExPASy Swiss-Prot
Match: UBIQ_LEITA (Ubiquitin OS=Leishmania tarentolae GN=UB-EP52 PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 6.757e-32
Identity = 69/76 (90.79%), Postives = 72/76 (94.74%), Query Frame = 1

Query:   70 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 297
            MQIFVKTLTG TI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFA KQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGTTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFADKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 137.502 bits (345), Expect = 6.757e-32
Identity = 69/76 (90.79%), Postives = 72/76 (94.74%), Query Frame = 1

Query:  298 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 525
            MQIFVKTLTG TI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFA KQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGTTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFADKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 3 Score: 83.5741 bits (205), Expect = 1.447e-16
Identity = 42/48 (87.50%), Postives = 43/48 (89.58%), Query Frame = 1

Query:  526 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQXRLIFAGK 669
            MQIFVKTLTG TI LEVE SDTI+NVKAKIQDKEGIPPDQ RLIFA K
Sbjct:    1 MQIFVKTLTGTTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFADK 48

HSP 4 Score: 21.9422 bits (45), Expect = 1.447e-16
Identity = 8/10 (80.00%), Postives = 10/10 (100.00%), Query Frame = 3

Query:  672 LEDGRTLADY 701
            LE+GRTL+DY
Sbjct:   50 LEEGRTLSDY 59

[Close]

BLAST of FC868749 vs. ExPASy Swiss-Prot
Match: UBIL_NPVOP (Ubiquitin-like protein OS=Orgyia pseudotsugata multicapsid polyhedrosis virus GN=V-UBI PE=3 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 3.470e-28
Identity = 61/76 (80.26%), Postives = 67/76 (88.16%), Query Frame = 1

Query:   70 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 297
            MQIFVKTLTGKTIT+E E  DT+  VK KI DKEG+P DQQRLI+AGKQLED +TLADYNIQKESTLH+VLRLRGG
Sbjct:    1 MQIFVKTLTGKTITVETEPGDTVGQVKQKIADKEGVPVDQQRLIYAGKQLEDAQTLADYNIQKESTLHMVLRLRGG 76

HSP 2 Score: 125.176 bits (313), Expect = 3.470e-28
Identity = 61/76 (80.26%), Postives = 67/76 (88.16%), Query Frame = 1

Query:  298 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 525
            MQIFVKTLTGKTIT+E E  DT+  VK KI DKEG+P DQQRLI+AGKQLED +TLADYNIQKESTLH+VLRLRGG
Sbjct:    1 MQIFVKTLTGKTITVETEPGDTVGQVKQKIADKEGVPVDQQRLIYAGKQLEDAQTLADYNIQKESTLHMVLRLRGG 76

HSP 3 Score: 73.1738 bits (178), Expect = 3.784e-13
Identity = 35/48 (72.92%), Postives = 39/48 (81.25%), Query Frame = 1

Query:  526 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQXRLIFAGK 669
            MQIFVKTLTGKTIT+E E  DT+  VK KI DKEG+P DQ RLI+AGK
Sbjct:    1 MQIFVKTLTGKTITVETEPGDTVGQVKQKIADKEGVPVDQQRLIYAGK 48

HSP 4 Score: 20.7866 bits (42), Expect = 3.784e-13
Identity = 8/10 (80.00%), Postives = 9/10 (90.00%), Query Frame = 3

Query:  672 LEDGRTLADY 701
            LED +TLADY
Sbjct:   50 LEDAQTLADY 59

[Close]

BLAST of FC868749 vs. ExPASy Swiss-Prot
Match: UBIL_NPVAC (Ubiquitin-like protein OS=Autographa californica nuclear polyhedrosis virus GN=V-UBI PE=3 SV=2)

HSP 1 Score: 122.479 bits (306), Expect = 2.249e-27
Identity = 58/76 (76.32%), Postives = 68/76 (89.47%), Query Frame = 1

Query:   70 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 297
            MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRLIFAGKQLED +T+ADYNIQKESTLH+VLRLRGG
Sbjct:    1 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 76

HSP 2 Score: 122.479 bits (306), Expect = 2.249e-27
Identity = 58/76 (76.32%), Postives = 68/76 (89.47%), Query Frame = 1

Query:  298 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 525
            MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRLIFAGKQLED +T+ADYNIQKESTLH+VLRLRGG
Sbjct:    1 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 76

HSP 3 Score: 70.8626 bits (172), Expect = 2.366e-12
Identity = 33/48 (68.75%), Postives = 40/48 (83.33%), Query Frame = 1

Query:  526 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQXRLIFAGK 669
            MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQ RLIFAGK
Sbjct:    1 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRLIFAGK 48

HSP 4 Score: 20.4014 bits (41), Expect = 2.366e-12
Identity = 7/10 (70.00%), Postives = 9/10 (90.00%), Query Frame = 3

Query:  672 LEDGRTLADY 701
            LED +T+ADY
Sbjct:   50 LEDSKTMADY 59

[Close]

BLAST of FC868749 vs. ExPASy Swiss-Prot
Match: UBIQ_LUMTE (Ubiquitin (Fragment) OS=Lumbricus terrestris PE=1 SV=2)

HSP 1 Score: 120.553 bits (301), Expect = 8.547e-27
Identity = 60/63 (95.24%), Postives = 62/63 (98.41%), Query Frame = 1

Query:  103 TITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLR 291
            TITLEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLR
Sbjct:    1 TITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLR 63

HSP 2 Score: 120.553 bits (301), Expect = 8.547e-27
Identity = 60/63 (95.24%), Postives = 62/63 (98.41%), Query Frame = 1

Query:  331 TITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLR 519
            TITLEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLR
Sbjct:    1 TITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLR 63

HSP 3 Score: 69.707 bits (169), Expect = 6.389e-13
Identity = 34/37 (91.89%), Postives = 35/37 (94.59%), Query Frame = 1

Query:  559 TITLEVESSDTIDNVKAKIQDKEGIPPDQXRLIFAGK 669
            TITLEVE SDTI+NVKAKIQDKEGIPPDQ RLIFAGK
Sbjct:    1 TITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGK 37

HSP 4 Score: 23.483 bits (49), Expect = 6.389e-13
Identity = 9/10 (90.00%), Postives = 10/10 (100.00%), Query Frame = 3

Query:  672 LEDGRTLADY 701
            LEDGRTL+DY
Sbjct:   39 LEDGRTLSDY 48

[Close]

BLAST of FC868749 vs. ExPASy Swiss-Prot
Match: RUB1_DESAN (NEDD8-like protein RUB1 OS=Deschampsia antarctica GN=RUB1 PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 2.039e-20
Identity = 48/74 (64.86%), Postives = 59/74 (79.73%), Query Frame = 1

Query:   76 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 297
            I VKTLTGK I +++E +DTID VK ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRVKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 99.3673 bits (246), Expect = 2.039e-20
Identity = 48/74 (64.86%), Postives = 59/74 (79.73%), Query Frame = 1

Query:  304 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 525
            I VKTLTGK I +++E +DTID VK ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRVKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

[Close]

BLAST of FC868749 vs. ExPASy Swiss-Prot
Match: RUB2_ORYSJ (NEDD8-like protein RUB2 OS=Oryza sativa subsp. japonica GN=RUB2 PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 2.663e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 1

Query:   76 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 297
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 98.9821 bits (245), Expect = 2.663e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 1

Query:  304 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 525
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

[Close]

BLAST of FC868749 vs. ExPASy Swiss-Prot
Match: RUB1_ORYSJ (NEDD8-like protein RUB1 OS=Oryza sativa subsp. japonica GN=RUB1 PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 2.663e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 1

Query:   76 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 297
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 98.9821 bits (245), Expect = 2.663e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 1

Query:  304 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 525
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

[Close]

BLAST of FC868749 vs. ExPASy Swiss-Prot
Match: RUB1_ARATH (NEDD8-like protein RUB1 OS=Arabidopsis thaliana GN=RUB1 PE=1 SV=2)

HSP 1 Score: 98.9821 bits (245), Expect = 2.663e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 1

Query:   76 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 297
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 98.9821 bits (245), Expect = 2.663e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 1

Query:  304 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 525
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

[Close]

BLAST of FC868749 vs. ExPASy Swiss-Prot
Match: NEDD8_DROME (NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 2.663e-20
Identity = 46/79 (58.23%), Postives = 60/79 (75.95%), Query Frame = 1

Query:   70 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQI 306
            M I VKTLTGK I +++E +D +D +K ++++KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG  I
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDSI 79

HSP 2 Score: 98.9821 bits (245), Expect = 2.663e-20
Identity = 46/79 (58.23%), Postives = 60/79 (75.95%), Query Frame = 1

Query:  298 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQI 534
            M I VKTLTGK I +++E +D +D +K ++++KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG  I
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDSI 79

The following BLAST results are available for this feature:

BLAST of FC868749 vs. ExPASy Swiss-Prot

BLAST of FC868749 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 99


Match Name	E-value	Identity	Description
UBIQ_LEIMA	2.100e-33	93.42	Ubiquitin OS=Leishmania major GN=UB-EP52 PE=3 SV=1	[more]
UBIQ_LEITA	6.757e-32	90.79	Ubiquitin OS=Leishmania tarentolae GN=UB-EP52 PE=3...	[more]
UBIL_NPVOP	3.470e-28	80.26	Ubiquitin-like protein OS=Orgyia pseudotsugata mul...	[more]
UBIL_NPVAC	2.249e-27	76.32	Ubiquitin-like protein OS=Autographa californica n...	[more]
UBIQ_LUMTE	8.547e-27	95.24	Ubiquitin (Fragment) OS=Lumbricus terrestris PE=1 ...	[more]
RUB1_DESAN	2.039e-20	64.86	NEDD8-like protein RUB1 OS=Deschampsia antarctica ...	[more]
RUB2_ORYSJ	2.663e-20	63.51	NEDD8-like protein RUB2 OS=Oryza sativa subsp. jap...	[more]
RUB1_ORYSJ	2.663e-20	63.51	NEDD8-like protein RUB1 OS=Oryza sativa subsp. jap...	[more]
RUB1_ARATH	2.663e-20	63.51	NEDD8-like protein RUB1 OS=Arabidopsis thaliana GN...	[more]
NEDD8_DROME	2.663e-20	58.23	NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=...	[more]

Pages

Properties


Property Name	Value
Genbank description	C31004C11EF AbioticL1 Citrus clementina cDNA clone C31004C11, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FC868749 ID=FC868749; Name=FC868749; organism=Citrus clementina; type=EST; length=707bp
GAAAAATTCTCAGATTATCTTCTCCTTTAATTCGATAAAAGGCCTCCTTC
TGTTCTCTCTGTCAAGCAGATGCAAATCTTTGTGAAAACTCTCACCGGCA
AGACAATAACCCTTGAGGTTGAAAGCTCCGACACCATTGATAATGTAAAA
GCCAAGATCCAGGACAAGGAAGGGATCCCACCAGATCAGCAGAGGCTTAT
CTTTGCTGGTAAACAGCTCGAGGATGGTCGCACACTCGCTGACTATAATA
TCCAGAAGGAATCAACCCTCCACTTGGTCCTTCGACTTAGGGGTGGTATG
CAGATCTTTGTAAAGACCCTCACTGGCAAGACGATTACTCTTGAGGTGGA
AAGCTCCGACACCATTGACAATGTGAAAGCCAAGATTCAAGACAAGGAAG
GGATCCCACCAGACCAACAGAGGCTTATCTTTGCTGGTAAACAGCTTGAG
GATGGTCGCACACTTGCTGATTATAACATCCAGAAGGAGTCAACCCTCCA
CTTGGTCCTCCGTCTCCGTGGTGGTATGCAGATCTTTGTAAAGACCCTCA
CTGGAAAGACTATCACCCTTGAGGTGGAAAGCTCCGACACCATTGACAAT
GTCAAAGCCAAAATTCAGGATAAGGAAGGGATCCCACCAGACCAGNCAAG
GCTCATCTTTGCCGGTAAACACTAGAGGATGGACGCACACTTGCAGACTA
CACATCC