FC869212

Overview
NameFC869212
Unique NameFC869212
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length401
Libraries
Library NameType
AbioticL1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC869212 vs. ExPASy Swiss-Prot
Match: HSP71_PICAN (Heat shock protein 70 1 OS=Pichia angusta GN=HSA1 PE=1 SV=2)

HSP 1 Score: 135.576 bits (340), Expect = 7.575e-32
Identity = 70/132 (53.03%), Postives = 92/132 (69.70%), Query Frame = 2
Query:   14 KVIENSEGSRTTPSVVAFNQKGELLVGTPAKRQAATNPTNTLFGTKRLIGRKFDDPQTQREMQMVSYKIVRA---PNGDAWVEANGQQYSPSQIGAFVLTKMKETAESYLGKSVSKAVITVPAYFNDAQRQA 400
            ++I N +G+RTTPS VAF    E L+G  AK QAA NP NT+F  KRLIGRKFDDP+ Q +++   +K+V     P+     +   + ++P +I + VLTKMKETAESY+G  V+ AVITVPAYFND+QRQA
Sbjct:   25 EIIANDQGNRTTPSFVAFTDT-ERLIGDAAKNQAAMNPANTVFDAKRLIGRKFDDPEVQNDIKHFPFKVVEKGGKPHIQVEFKGETKVFTPEEISSMVLTKMKETAESYMGGKVTDAVITVPAYFNDSQRQA 155          
BLAST of FC869212 vs. ExPASy Swiss-Prot
Match: HSP71_ANOAL (Heat shock protein 70 A1 OS=Anopheles albimanus GN=HSP70A1 PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 7.575e-32
Identity = 73/132 (55.30%), Postives = 94/132 (71.21%), Query Frame = 2
Query:   14 KVIENSEGSRTTPSVVAFNQKGELLVGTPAKRQAATNPTNTLFGTKRLIGRKFDDPQTQREMQMVSYKIVR-APNGDAWVEANGQQ--YSPSQIGAFVLTKMKETAESYLGKSVSKAVITVPAYFNDAQRQA 400
            ++I N +G+RTTPS VAF+   E L+G  AK Q A NPTNT+F  KRLIGRKFDDP+ Q +M+   + +V         VE  G++  ++P +I + VLTKMKETAE+YLG+SV  AVITVPAYFND+QRQA
Sbjct:   25 EIIANDQGNRTTPSYVAFSDT-ERLIGDAAKNQVAMNPTNTVFDAKRLIGRKFDDPKIQADMKHWPFTVVNDCGKPKIRVEFKGERKTFAPEEISSMVLTKMKETAEAYLGQSVKNAVITVPAYFNDSQRQA 155          
BLAST of FC869212 vs. ExPASy Swiss-Prot
Match: GRP78_SPETR (78 kDa glucose-regulated protein OS=Spermophilus tridecemlineatus GN=HSPA5 PE=2 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 7.575e-32
Identity = 69/133 (51.88%), Postives = 94/133 (70.68%), Query Frame = 2
Query:   14 KVIENSEGSRTTPSVVAFNQKGELLVGTPAKRQAATNPTNTLFGTKRLIGRKFDDPQTQREMQMVSYKIV-RAPNGDAWVEANGQQ---YSPSQIGAFVLTKMKETAESYLGKSVSKAVITVPAYFNDAQRQA 400
            ++I N +G+R TPS VAF  +GE L+G  AK Q  +NP NT+F  KRLIGR ++DP  Q++++ + +K+V +       V+  G Q   ++P +I A VLTKMKETAE+YLGK V+ AV+TVPAYFNDAQRQA
Sbjct:   51 EIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQA 183          
BLAST of FC869212 vs. ExPASy Swiss-Prot
Match: GRP78_RAT (78 kDa glucose-regulated protein OS=Rattus norvegicus GN=Hspa5 PE=1 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 7.575e-32
Identity = 69/133 (51.88%), Postives = 94/133 (70.68%), Query Frame = 2
Query:   14 KVIENSEGSRTTPSVVAFNQKGELLVGTPAKRQAATNPTNTLFGTKRLIGRKFDDPQTQREMQMVSYKIV-RAPNGDAWVEANGQQ---YSPSQIGAFVLTKMKETAESYLGKSVSKAVITVPAYFNDAQRQA 400
            ++I N +G+R TPS VAF  +GE L+G  AK Q  +NP NT+F  KRLIGR ++DP  Q++++ + +K+V +       V+  G Q   ++P +I A VLTKMKETAE+YLGK V+ AV+TVPAYFNDAQRQA
Sbjct:   51 EIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQA 183          
BLAST of FC869212 vs. ExPASy Swiss-Prot
Match: GRP78_MOUSE (78 kDa glucose-regulated protein OS=Mus musculus GN=Hspa5 PE=1 SV=3)

HSP 1 Score: 135.576 bits (340), Expect = 7.575e-32
Identity = 69/133 (51.88%), Postives = 94/133 (70.68%), Query Frame = 2
Query:   14 KVIENSEGSRTTPSVVAFNQKGELLVGTPAKRQAATNPTNTLFGTKRLIGRKFDDPQTQREMQMVSYKIV-RAPNGDAWVEANGQQ---YSPSQIGAFVLTKMKETAESYLGKSVSKAVITVPAYFNDAQRQA 400
            ++I N +G+R TPS VAF  +GE L+G  AK Q  +NP NT+F  KRLIGR ++DP  Q++++ + +K+V +       V+  G Q   ++P +I A VLTKMKETAE+YLGK V+ AV+TVPAYFNDAQRQA
Sbjct:   52 EIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQA 184          
BLAST of FC869212 vs. ExPASy Swiss-Prot
Match: GRP78_MESAU (78 kDa glucose-regulated protein OS=Mesocricetus auratus GN=HSPA5 PE=1 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 7.575e-32
Identity = 69/133 (51.88%), Postives = 94/133 (70.68%), Query Frame = 2
Query:   14 KVIENSEGSRTTPSVVAFNQKGELLVGTPAKRQAATNPTNTLFGTKRLIGRKFDDPQTQREMQMVSYKIV-RAPNGDAWVEANGQQ---YSPSQIGAFVLTKMKETAESYLGKSVSKAVITVPAYFNDAQRQA 400
            ++I N +G+R TPS VAF  +GE L+G  AK Q  +NP NT+F  KRLIGR ++DP  Q++++ + +K+V +       V+  G Q   ++P +I A VLTKMKETAE+YLGK V+ AV+TVPAYFNDAQRQA
Sbjct:   51 EIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQA 183          
BLAST of FC869212 vs. ExPASy Swiss-Prot
Match: GRP78_HUMAN (78 kDa glucose-regulated protein OS=Homo sapiens GN=HSPA5 PE=1 SV=2)

HSP 1 Score: 135.576 bits (340), Expect = 7.575e-32
Identity = 69/133 (51.88%), Postives = 94/133 (70.68%), Query Frame = 2
Query:   14 KVIENSEGSRTTPSVVAFNQKGELLVGTPAKRQAATNPTNTLFGTKRLIGRKFDDPQTQREMQMVSYKIV-RAPNGDAWVEANGQQ---YSPSQIGAFVLTKMKETAESYLGKSVSKAVITVPAYFNDAQRQA 400
            ++I N +G+R TPS VAF  +GE L+G  AK Q  +NP NT+F  KRLIGR ++DP  Q++++ + +K+V +       V+  G Q   ++P +I A VLTKMKETAE+YLGK V+ AV+TVPAYFNDAQRQA
Sbjct:   51 EIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQA 183          
BLAST of FC869212 vs. ExPASy Swiss-Prot
Match: DNAK_CAMHC (Chaperone protein dnaK OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) GN=dnaK PE=2 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 7.575e-32
Identity = 71/129 (55.04%), Postives = 88/129 (68.22%), Query Frame = 2
Query:   14 KVIENSEGSRTTPSVVAFNQKGELLVGTPAKRQAATNPTNTLFGTKRLIGRKFDDPQTQREMQMVSYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKETAESYLGKSVSKAVITVPAYFNDAQRQA 400
            K+I N EG  TTPSVVAF  KGE+LVG  AKRQA TNP  T++  KR++G   ++   Q   + + YKIV   NG   VE  G+ Y+P +I A VL K+KE AESYLG+ V  AVITVPAYFND+QR+A
Sbjct:   25 KIIPNKEGKNTTPSVVAFTDKGEILVGDSAKRQAVTNPEKTIYSIKRIMGLMMNEKNAQEAKKRLPYKIVDR-NGACAVEIAGKVYTPQEISAKVLMKLKEDAESYLGEKVVDAVITVPAYFNDSQRKA 152          
BLAST of FC869212 vs. ExPASy Swiss-Prot
Match: HSP72_PARBA (Heat shock 70 kDa protein 2 OS=Paracoccidioides brasiliensis (strain ATCC MYA-826 / Pb01) GN=HSP70-2 PE=2 SV=2)

HSP 1 Score: 134.806 bits (338), Expect = 1.292e-31
Identity = 71/132 (53.79%), Postives = 95/132 (71.97%), Query Frame = 2
Query:   14 KVIENSEGSRTTPSVVAFNQKGELLVGTPAKRQAATNPTNTLFGTKRLIGRKFDDPQTQREMQMVSYKIV-RAPNGDAWVEANGQ--QYSPSQIGAFVLTKMKETAESYLGKSVSKAVITVPAYFNDAQRQA 400
            ++I N +G+RTTPS VAF    E L+G  AK Q A NP+NT+F  KRLIGRKF DP+ Q +M+   +K++ +A      VE  G+  Q++P +I + VLTKM+ETAESYLG +V+ AV+TVPAYFND+QRQA
Sbjct:   25 EIIANDQGNRTTPSFVAFTDT-ERLIGDAAKNQVAMNPSNTVFDAKRLIGRKFADPEVQSDMKHFPFKVIDKAGKPVISVEFKGEEKQFTPEEISSMVLTKMRETAESYLGGTVNNAVVTVPAYFNDSQRQA 155          
BLAST of FC869212 vs. ExPASy Swiss-Prot
Match: DNAK2_THEEB (Chaperone protein dnaK2 OS=Thermosynechococcus elongatus (strain BP-1) GN=dnaK2 PE=2 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 1.292e-31
Identity = 73/135 (54.07%), Postives = 94/135 (69.63%), Query Frame = 2
Query:    2 GKNPKVIENSEGSRTTPSVVAFNQKGELLVGTPAKRQAATNPTNTLFGTKRLIGRKFDDPQTQREMQMVSYKIVRAPNGDAWVE--ANGQQYSPSQIGAFVLTKMKETAESYLGKSVSKAVITVPAYFNDAQRQA 400
            G  P VI N+EG RTTPSVVA+ + G+ LVG  AKRQA  NP NT +  KR IGR+FD  +   E   VSYK++   NG+  ++  A G+Q++P +I A VL K+KE A  YLG+ V++AVITVPAYFND+QRQA
Sbjct:   21 GGKPTVIANAEGFRTTPSVVAYTKNGDRLVGQIAKRQAVMNPENTFYSVKRFIGRRFD--EVTHEATEVSYKVLNV-NGNVKLDCPALGKQFAPEEISAQVLRKLKEDASKYLGEEVTQAVITVPAYFNDSQRQA 152          
The following BLAST results are available for this feature:
BLAST of FC869212 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
HSP71_PICAN7.575e-3253.03Heat shock protein 70 1 OS=Pichia angusta GN=HSA1 ... [more]
HSP71_ANOAL7.575e-3255.30Heat shock protein 70 A1 OS=Anopheles albimanus GN... [more]
GRP78_SPETR7.575e-3251.8878 kDa glucose-regulated protein OS=Spermophilus t... [more]
GRP78_RAT7.575e-3251.8878 kDa glucose-regulated protein OS=Rattus norvegi... [more]
GRP78_MOUSE7.575e-3251.8878 kDa glucose-regulated protein OS=Mus musculus G... [more]
GRP78_MESAU7.575e-3251.8878 kDa glucose-regulated protein OS=Mesocricetus a... [more]
GRP78_HUMAN7.575e-3251.8878 kDa glucose-regulated protein OS=Homo sapiens G... [more]
DNAK_CAMHC7.575e-3255.04Chaperone protein dnaK OS=Campylobacter hominis (s... [more]
HSP72_PARBA1.292e-3153.79Heat shock 70 kDa protein 2 OS=Paracoccidioides br... [more]
DNAK2_THEEB1.292e-3154.07Chaperone protein dnaK2 OS=Thermosynechococcus elo... [more]

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Properties
Property NameValue
Genbank descriptionC31009G02EF AbioticL1 Citrus clementina cDNA clone C31009G02, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC869212 ID=FC869212; Name=FC869212; organism=Citrus clementina; type=EST; length=401bp
GGGAAAGAACCCCAAAGTTATTGAGAACTCCGAAGGATCTAGGACTACAC
CATCAGTTGTAGCTTTCAATCAGAAGGGAGAGCTACTTGTGGGTACACCA
GCAAAGCGTCAGGCTGCGACCAACCCGACTAACACGCTCTTTGGCACCAA
GCGTTTAATTGGTAGAAAATTTGATGATCCCCAGACGCAAAGAGAAATGC
AGATGGTTTCATATAAGATAGTAAGGGCCCCCAATGGGGATGCTTGGGTT
GAAGCCAATGGGCAGCAGTACTCGCCAAGTCAGATTGGGGCCTTTGTTCT
GACCAAGATGAAAGAAACTGCAGAATCCTACCTTGGAAAGTCAGTTTCAA
AAGCAGTAATCACAGTTCCAGCTTATTTCAACGATGCTCAAAGGCAAGCT
A
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