FC869225

Overview
NameFC869225
Unique NameFC869225
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length756
Libraries
Library NameType
AbioticL1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of FC869225 vs. ExPASy Swiss-Prot
Match: HEM3_CLOBM (Porphobilinogen deaminase OS=Clostridium botulinum (strain Loch Maree / Type A3) GN=hemC PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 5.812e-16
Identity = 53/135 (39.26%), Postives = 74/135 (54.81%), Query Frame = 3 
Query:  339 IGTRGSPLALAQAHETRNKLMALHPELAQEGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDEALINSQIDIAVHSMKDVPTYLPEKTILPCNLQREDVRDAFIXXXXXXXXXXPAGSIVGTASLRRSLKYSI 743
            I TR S LA  Q     + L   H    ++     ++I+T GDKIL   L  IGGKGLF K+I+ A++  + D AVHSMKDVP  +P+   +    +REDVRDAFI            G+ +GT+S RR+ +  +
Sbjct:    5 IATRRSKLAQVQTEIIIDLLNKKHDIECEK-----LLIETVGDKILEVSLDKIGGKGLFVKDIEVAMLEQRADAAVHSMKDVPYEMPKGFEIIAIPEREDVRDAFISLDNIKFKDLREGAKIGTSSRRRAAQLKL 134
[Close]
BLAST of FC869225 vs. ExPASy Swiss-Prot
Match: HEM3_CLOBK (Porphobilinogen deaminase OS=Clostridium botulinum (strain Okra / Type B1) GN=hemC PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 5.812e-16
Identity = 53/135 (39.26%), Postives = 74/135 (54.81%), Query Frame = 3 
Query:  339 IGTRGSPLALAQAHETRNKLMALHPELAQEGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDEALINSQIDIAVHSMKDVPTYLPEKTILPCNLQREDVRDAFIXXXXXXXXXXPAGSIVGTASLRRSLKYSI 743
            I TR S LA  Q     + L   H    ++     ++I+T GDKIL   L  IGGKGLF K+I+ A++  + D AVHSMKDVP  +P+   +    +REDVRDAFI            G+ +GT+S RR+ +  +
Sbjct:    5 IATRRSKLAQVQTEIIIDLLNKKHDIECEK-----LLIETVGDKILEVSLDKIGGKGLFVKDIEVAMLEQRADAAVHSMKDVPYEMPKGFEIIAIPEREDVRDAFISLDNIKFKDLRKGAKIGTSSRRRAAQLKL 134
[Close]
BLAST of FC869225 vs. ExPASy Swiss-Prot
Match: HEM3_CLOBJ (Porphobilinogen deaminase OS=Clostridium botulinum (strain Kyoto / Type A2) GN=hemC PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 5.812e-16
Identity = 53/135 (39.26%), Postives = 74/135 (54.81%), Query Frame = 3 
Query:  339 IGTRGSPLALAQAHETRNKLMALHPELAQEGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDEALINSQIDIAVHSMKDVPTYLPEKTILPCNLQREDVRDAFIXXXXXXXXXXPAGSIVGTASLRRSLKYSI 743
            I TR S LA  Q     + L   H    ++     ++I+T GDKIL   L  IGGKGLF K+I+ A++  + D AVHSMKDVP  +P+   +    +REDVRDAFI            G+ +GT+S RR+ +  +
Sbjct:    5 IATRRSKLAQVQTEIIIDLLNKKHDIECEK-----LLIETVGDKILEVSLDKIGGKGLFVKDIEVAMLEQRADAAVHSMKDVPYEMPKGFEIIAIPEREDVRDAFISLDNIKFKDLRKGAKIGTSSRRRAAQLKL 134
[Close]
BLAST of FC869225 vs. ExPASy Swiss-Prot
Match: HEM3_CLOBH (Porphobilinogen deaminase OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) GN=hemC PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 5.812e-16
Identity = 53/135 (39.26%), Postives = 74/135 (54.81%), Query Frame = 3 
Query:  339 IGTRGSPLALAQAHETRNKLMALHPELAQEGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDEALINSQIDIAVHSMKDVPTYLPEKTILPCNLQREDVRDAFIXXXXXXXXXXPAGSIVGTASLRRSLKYSI 743
            I TR S LA  Q     + L   H    ++     ++I+T GDKIL   L  IGGKGLF K+I+ A++  + D AVHSMKDVP  +P+   +    +REDVRDAFI            G+ +GT+S RR+ +  +
Sbjct:    5 IATRRSKLAQVQTEIIIDLLNKKHDIECEK-----LLIETVGDKILEVSLDKIGGKGLFVKDIEVAMLEQRADAAVHSMKDVPYEMPKGFEIIAIPEREDVRDAFISLDNIKFKDLRKGAKIGTSSRRRAAQLKL 134
[Close]
BLAST of FC869225 vs. ExPASy Swiss-Prot
Match: HEM3_CLOB6 (Porphobilinogen deaminase OS=Clostridium botulinum (strain 657 / Type Ba4) GN=hemC PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 5.812e-16
Identity = 53/135 (39.26%), Postives = 74/135 (54.81%), Query Frame = 3 
Query:  339 IGTRGSPLALAQAHETRNKLMALHPELAQEGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDEALINSQIDIAVHSMKDVPTYLPEKTILPCNLQREDVRDAFIXXXXXXXXXXPAGSIVGTASLRRSLKYSI 743
            I TR S LA  Q     + L   H    ++     ++I+T GDKIL   L  IGGKGLF K+I+ A++  + D AVHSMKDVP  +P+   +    +REDVRDAFI            G+ +GT+S RR+ +  +
Sbjct:    5 IATRRSKLAQVQTEIIIDLLNKKHDIECEK-----LLIETVGDKILEVSLDKIGGKGLFVKDIEVAMLEQRADAAVHSMKDVPYEMPKGFEIIAIPEREDVRDAFISLDNIKFKDLREGAKIGTSSRRRAAQLKL 134
[Close]
BLAST of FC869225 vs. ExPASy Swiss-Prot
Match: HEM3_CLOB1 (Porphobilinogen deaminase OS=Clostridium botulinum (strain ATCC 19397 / Type A) GN=hemC PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 5.812e-16
Identity = 53/135 (39.26%), Postives = 74/135 (54.81%), Query Frame = 3 
Query:  339 IGTRGSPLALAQAHETRNKLMALHPELAQEGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDEALINSQIDIAVHSMKDVPTYLPEKTILPCNLQREDVRDAFIXXXXXXXXXXPAGSIVGTASLRRSLKYSI 743
            I TR S LA  Q     + L   H    ++     ++I+T GDKIL   L  IGGKGLF K+I+ A++  + D AVHSMKDVP  +P+   +    +REDVRDAFI            G+ +GT+S RR+ +  +
Sbjct:    5 IATRRSKLAQVQTEIIIDLLNKKHDIECEK-----LLIETVGDKILEVSLDKIGGKGLFVKDIEVAMLEQRADAAVHSMKDVPYEMPKGFEIIAIPEREDVRDAFISLDNIKFKDLRKGAKIGTSSRRRAAQLKL 134
[Close]
BLAST of FC869225 vs. ExPASy Swiss-Prot
Match: HEM3_PICTO (Probable porphobilinogen deaminase OS=Picrophilus torridus GN=hemC PE=3 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 7.591e-16
Identity = 49/131 (37.40%), Postives = 75/131 (57.25%), Query Frame = 3 
Query:  333 IRIGTRGSPLALAQAHETRNKLMALHPELAQEGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDEALINSQIDIAVHSMKDVPTYLPEKTILPCNLQREDVRDAFIXXXXXXXXXXPAGSIVGTASLRR 725
            IRIGTR S LA+ QA   +++L +L  E   +G        + GD  L  PL  IGG G+F  +++  ++ ++IDIAVHS KD+P+++ +   +   L+R+D RD  I           A S++GT+SLRR
Sbjct:    3 IRIGTRSSKLAMIQAMMVKDRLDSLGIETEVKG------FTSKGDINLDSPLYSIGGTGVFVDDLNRMILKNEIDIAVHSAKDIPSFIDDSLEISAVLKRDDPRDVLI--SQHSLNDLEASSVIGTSSLRR 125
[Close]
BLAST of FC869225 vs. ExPASy Swiss-Prot
Match: HEM3_CLOBL (Porphobilinogen deaminase OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) GN=hemC PE=3 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 7.591e-16
Identity = 53/135 (39.26%), Postives = 74/135 (54.81%), Query Frame = 3 
Query:  339 IGTRGSPLALAQAHETRNKLMALHPELAQEGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDEALINSQIDIAVHSMKDVPTYLPEKTILPCNLQREDVRDAFIXXXXXXXXXXPAGSIVGTASLRRSLKYSI 743
            I TR S LA  Q     + L   H    ++     ++I+T GDKIL   L  IGGKGLF K+I+ A++  + D AVHSMKDVP  +P+   +    +REDVRDAFI            G+ +GT+S RR+ +  +
Sbjct:    5 IATRRSKLAQVQTEIIIDLLNKKHDIECEK-----LLIETVGDKILEVSLDKIGGKGLFVKDIEVAMLEQRADAAVHSMKDVPYEMPKGFEIIAIPKREDVRDAFISLDNIKFKDLRKGAKIGTSSRRRAAQLKL 134
[Close]
BLAST of FC869225 vs. ExPASy Swiss-Prot
Match: HEM3_THET8 (Porphobilinogen deaminase OS=Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) GN=hemC PE=3 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 9.914e-16
Identity = 52/132 (39.39%), Postives = 73/132 (55.30%), Query Frame = 3 
Query:  330 IIRIGTRGSPLALAQAHETRNKLMALHPELAQEGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDEALINSQIDIAVHSMKDVPTYLPEKTILPCNLQREDVRDAFIXXXXXXXXXXPAGSIVGTASLRR 725
            ++ +GTRGS LALAQ      +L    PE       +I  IKT GD+  S        + +F KEI EAL++ +IDIAVHS+KD+PT  P    +    +R+D RD F+          P G++VGT+S+RR
Sbjct:    3 VVVVGTRGSALALAQTRFVVERLKETWPETE----FKIRTIKTRGDQGASPR-----EEAIFVKEIQEALLSREIDIAVHSLKDLPTESPRGLKIASVPRRQDPRDVFLGKAAKRLKDLPPGAVVGTSSVRR 125
[Close]
BLAST of FC869225 vs. ExPASy Swiss-Prot
Match: HEM3_THET2 (Porphobilinogen deaminase OS=Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) GN=hemC PE=3 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 9.914e-16
Identity = 52/132 (39.39%), Postives = 73/132 (55.30%), Query Frame = 3 
Query:  330 IIRIGTRGSPLALAQAHETRNKLMALHPELAQEGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDEALINSQIDIAVHSMKDVPTYLPEKTILPCNLQREDVRDAFIXXXXXXXXXXPAGSIVGTASLRR 725
            ++ +GTRGS LALAQ      +L    PE       +I  IKT GD+  S        + +F KEI EAL++ +IDIAVHS+KD+PT  P    +    +R+D RD F+          P G++VGT+S+RR
Sbjct:    3 VVVVGTRGSALALAQTRFVVERLKETWPETE----FKIRTIKTRGDQGASPR-----EEAIFVKEIQEALLSREIDIAVHSLKDLPTESPRGLKIASVPRRQDPRDVFLGKAAKRLKDLPPGAVVGTSSVRR 125
The following BLAST results are available for this feature:
BLAST of FC869225 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 460
Match NameE-valueIdentityDescription
HEM3_CLOBM5.812e-1639.26Porphobilinogen deaminase OS=Clostridium botulinum... [more]
HEM3_CLOBK5.812e-1639.26Porphobilinogen deaminase OS=Clostridium botulinum... [more]
HEM3_CLOBJ5.812e-1639.26Porphobilinogen deaminase OS=Clostridium botulinum... [more]
HEM3_CLOBH5.812e-1639.26Porphobilinogen deaminase OS=Clostridium botulinum... [more]
HEM3_CLOB65.812e-1639.26Porphobilinogen deaminase OS=Clostridium botulinum... [more]
HEM3_CLOB15.812e-1639.26Porphobilinogen deaminase OS=Clostridium botulinum... [more]
HEM3_PICTO7.591e-1637.40Probable porphobilinogen deaminase OS=Picrophilus ... [more]
HEM3_CLOBL7.591e-1639.26Porphobilinogen deaminase OS=Clostridium botulinum... [more]
HEM3_THET89.914e-1639.39Porphobilinogen deaminase OS=Thermus thermophilus ... [more]
HEM3_THET29.914e-1639.39Porphobilinogen deaminase OS=Thermus thermophilus ... [more]

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Properties
Property NameValue
Genbank descriptionC31009H04EF AbioticL1 Citrus clementina cDNA clone C31009H04, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC869225 ID=FC869225; Name=FC869225; organism=Citrus clementina; type=EST; length=756bp
GGAAACCAAAACATTACCAATCTCCGCTTCTAACTCGCTTTCTTCTTTTC
TTCTTTCATAAATAATTAGCACACTGTCTGTCCCCGTCTCTGTTTCTGAT
ACTGGGCAGTCATCATTGTTGTTGTGTGTCAATGGAGAGTTTGAGTTTAG
CAGCTTCTTCTCTTTGTACAAGCCGGGGTGGCCCCATGGAGCGCCATTGC
TATCCAGGGTCGGTCTCCCTTGTTGGGTTCTCTTCGCCACGCCTCAAGAC
TCCGTCTTTTCCAAAGAGTTTGAAGAAACGCAGTTTTGGGCTTGTAAGAG
CCTCTGCTGCTGTTGAAACCAAGGTTGCAATCATCAGAATTGGCACCAGA
GGAAGCCCTCTGGCACTTGCTCAGGCTCATGAGACGCGAAACAAACTCAT
GGCTTTGCATCCAGAGCTAGCTCAAGAGGGGGCGATTCAAATTGTAATAA
TAAAGACAACTGGTGATAAGATACTAAGTCAACCACTTGCAGACATTGGT
GGGAAGGGCTTGTTCACCAAAGAAATAGACGAGGCGCTGATAAATAGTCA
AATTGACATCGCCGTGCACTCGATGAAAGATGTTCCAACATATTTACCAG
AGAAGACCATTCTACCTTGCAACCTTCAGCGGGAGGATGTCCGAGATGCA
TTTATTTCGTTGAGTGCAGCCTCTTTAGCAGAACTACCAGCAGGAAGCAT
TGTTGGTACTGCTTCACTTAGAAGAAGTCTCAAATACTCCATAGATATCC
ATCATT
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