FC876438

Overview
NameFC876438
Unique NameFC876438
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length576
Libraries
Library NameType
DevOvary1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of FC876438 vs. ExPASy Swiss-Prot
Match: METK5_CAEEL (Probable S-adenosylmethionine synthetase 5 OS=Caenorhabditis elegans GN=sams-5 PE=2 SV=2)

HSP 1 Score: 146.747 bits (369), Expect = 8.214e-37
Identity = 72/121 (59.50%), Postives = 91/121 (75.21%), Query Frame = 2 
Query:   89 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPDSKVACETCTKTNLVMVFGEITTKANVNYEKIVRDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLSKRPEEIGAGDSG 451
            FLFTSESV+EGHPDK+CDQISDA+LDA L QDP +KVACET TKT ++M+ GEIT+KA V+Y+ +VR+  + IG+     G D   C VLV +EQQSP+IA GV  H+ K   ++GAGD G
Sbjct:    6 FLFTSESVSEGHPDKMCDQISDAVLDAHLAQDPHAKVACETVTKTGMIMLCGEITSKAVVDYQVLVRNVIKKIGYDDSSKGFDYKTCNVLVALEQQSPEIAAGV--HVDKDIADVGAGDQG 124

HSP 2 Score: 28.1054 bits (61), Expect = 8.214e-37
Identity = 12/14 (85.71%), Postives = 12/14 (85.71%), Query Frame = 3 
Query:  447 QGHMFGYATDETPE 488
            QG MFGYATDET E
Sbjct:  123 QGIMFGYATDETEE 136
[Close]
BLAST of FC876438 vs. ExPASy Swiss-Prot
Match: METK_PROMH (S-adenosylmethionine synthetase OS=Proteus mirabilis (strain HI4320) GN=metK PE=3 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 8.478e-37
Identity = 77/124 (62.10%), Postives = 91/124 (73.39%), Query Frame = 2 
Query:   80 METFLFTSESVNEGHPDKLCDQISDAILDACLEQDPDSKVACETCTKTNLVMVFGEITTKANVNYEKIVRDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLSKRPEEIGAGDSG 451
            M T LFTSESV+EGHPDK+ DQISDA+LDA LEQDP ++VACET  KT +VMV GEITTKA V+ E+I R T R IG+ S D+G DA++C V+  I +QSPDI QGV       P E GAGD G
Sbjct:    1 MTTHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEITTKAWVDIEEITRKTVREIGYTSSDMGFDANSCAVISAIGKQSPDINQGVD---RADPLEQGAGDQG 121

HSP 2 Score: 24.6386 bits (52), Expect = 8.478e-37
Identity = 10/12 (83.33%), Postives = 11/12 (91.67%), Query Frame = 3 
Query:  447 QGHMFGYATDET 482
            QG MFGYAT+ET
Sbjct:  120 QGLMFGYATNET 131

HSP 3 Score: 24.6386 bits (52), Expect = 8.478e-37
Identity = 10/21 (47.62%), Postives = 14/21 (66.67%), Query Frame = 1 
Query:  514 ATQLGAKLTEVRKNGTCTWMR 576
            A +L  +  +VRKNGT  W+R
Sbjct:  142 AHRLVQRQAQVRKNGTLPWLR 162
[Close]
BLAST of FC876438 vs. ExPASy Swiss-Prot
Match: METK_LACSS (S-adenosylmethionine synthetase OS=Lactobacillus sakei subsp. sakei (strain 23K) GN=metK PE=3 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 1.816e-36
Identity = 74/129 (57.36%), Postives = 92/129 (71.32%), Query Frame = 2 
Query:   83 ETFLFTSESVNEGHPDKLCDQISDAILDACLEQDPDSKVACETCTKTNLVMVFGEITTKANVNYEKIVRDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLSKRP------EEIGAGDSG 451
            E  LFTSESV+EGHPDK+ DQISDAILDA LEQDP ++VACET   T LV+V GEI+T A V+ +K+VR+T + IG+     G D DNC V+V +++QSPDIAQGV   L  R       ++IGAGD G
Sbjct:    3 ERHLFTSESVSEGHPDKIADQISDAILDAMLEQDPQARVACETTVTTGLVLVVGEISTTAYVDIQKVVRETIKKIGYRDGQYGFDGDNCAVIVALDEQSPDIAQGVDDSLESREKDEDPLDKIGAGDQG 131

HSP 2 Score: 26.9498 bits (58), Expect = 1.816e-36
Identity = 10/16 (62.50%), Postives = 13/16 (81.25%), Query Frame = 3 
Query:  447 QGHMFGYATDETPEFI 494
            QG MFG+A D+TPE +
Sbjct:  130 QGLMFGFAVDQTPELM 145
[Close]
BLAST of FC876438 vs. ExPASy Swiss-Prot
Match: METK_PROMT (S-adenosylmethionine synthetase OS=Prochlorococcus marinus (strain NATL2A) GN=metK PE=3 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 2.365e-36
Identity = 70/128 (54.69%), Postives = 95/128 (74.22%), Query Frame = 2 
Query:   80 METFLFTSESVNEGHPDKLCDQISDAILDACLEQDPDSKVACETCTKTNLVMVFGEITTKANVNYEKIVRDTCRNIGFVS-DDVGLDADNCKVLVYIEQQSPDIAQGVH---GHLSKRPEEIGAGDSG 451
            M  ++FTSESV EGHPDK+CDQ+SDA+LDACL +DP S+VACE    T L ++ GEIT+KA V++ K+VR+  +NIG+ S  D G DA++C VLV ++QQS DIAQGV+    H +   +++GAGD G
Sbjct:    1 MSRYVFTSESVTEGHPDKICDQVSDAVLDACLSEDPTSRVACEAVVNTGLCLITGEITSKAEVDFNKLVREVIKNIGYRSASDGGFDANSCAVLVALDQQSSDIAQGVNEAEDHSTDPLDQVGAGDQG 128

HSP 2 Score: 27.7202 bits (60), Expect = 2.365e-36
Identity = 11/16 (68.75%), Postives = 13/16 (81.25%), Query Frame = 3 
Query:  447 QGHMFGYATDETPEFI 494
            QG MFG+A DETPE +
Sbjct:  127 QGIMFGFACDETPELM 142
[Close]
BLAST of FC876438 vs. ExPASy Swiss-Prot
Match: METK_PHOLL (S-adenosylmethionine synthetase OS=Photorhabdus luminescens subsp. laumondii GN=metK PE=3 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 2.418e-36
Identity = 75/124 (60.48%), Postives = 93/124 (75.00%), Query Frame = 2 
Query:   80 METFLFTSESVNEGHPDKLCDQISDAILDACLEQDPDSKVACETCTKTNLVMVFGEITTKANVNYEKIVRDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLSKRPEEIGAGDSG 451
            M T+LFTSESV+EGHPDK+ DQISDA+LDA LEQDP ++VACET  KT +VMV GEITT A V+ E+I R T R IG+V+ ++G DA++C VL  I +QSPDI QGV     + P + GAGD G
Sbjct:    1 MTTYLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEITTSAWVDIEEITRQTVREIGYVNSEMGFDANSCAVLSAIGKQSPDINQGVD---REDPLQQGAGDQG 121

HSP 2 Score: 24.6386 bits (52), Expect = 2.418e-36
Identity = 10/12 (83.33%), Postives = 11/12 (91.67%), Query Frame = 3 
Query:  447 QGHMFGYATDET 482
            QG MFGYAT+ET
Sbjct:  120 QGLMFGYATNET 131

HSP 3 Score: 23.8682 bits (50), Expect = 2.418e-36
Identity = 10/21 (47.62%), Postives = 13/21 (61.90%), Query Frame = 1 
Query:  514 ATQLGAKLTEVRKNGTCTWMR 576
            A +L  +  EVRKNG   W+R
Sbjct:  142 AHRLVQRQAEVRKNGVLPWLR 162
[Close]
BLAST of FC876438 vs. ExPASy Swiss-Prot
Match: METK_METFK (S-adenosylmethionine synthetase OS=Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) GN=metK PE=3 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 3.083e-36
Identity = 74/124 (59.68%), Postives = 94/124 (75.81%), Query Frame = 2 
Query:   80 METFLFTSESVNEGHPDKLCDQISDAILDACLEQDPDSKVACETCTKTNLVMVFGEITTKANVNYEKIVRDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLSKRPEEIGAGDSG 451
            M  +LFTSESV+EGHPDK+ DQISD+ILDA LEQDP ++VA ET T T LV++ GEITT ANV+Y KI RDT + IG+ + D G+D  +C VLV  ++QSPDIAQGV+ + +  P + GAGD G
Sbjct:    1 MSEYLFTSESVSEGHPDKVADQISDSILDAILEQDPTARVAAETLTNTGLVVLAGEITTTANVDYIKIARDTIKRIGYDNADYGIDYKSCAVLVAYDKQSPDIAQGVN-NANDDPLDQGAGDQG 123

HSP 2 Score: 28.4906 bits (62), Expect = 3.083e-36
Identity = 11/16 (68.75%), Postives = 13/16 (81.25%), Query Frame = 3 
Query:  447 QGHMFGYATDETPEFI 494
            QG MFGYA DETP+ +
Sbjct:  122 QGLMFGYACDETPQLL 137
[Close]
BLAST of FC876438 vs. ExPASy Swiss-Prot
Match: METK_ALISL (S-adenosylmethionine synthetase OS=Aliivibrio salmonicida (strain LFI1238) GN=metK PE=3 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 4.016e-36
Identity = 75/124 (60.48%), Postives = 92/124 (74.19%), Query Frame = 2 
Query:   80 METFLFTSESVNEGHPDKLCDQISDAILDACLEQDPDSKVACETCTKTNLVMVFGEITTKANVNYEKIVRDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLSKRPEEIGAGDSG 451
            M   LFTSESV+EGHPDK+ DQISDA+LDA LEQDP ++VACET  KT +VMV GE+TT A V+ E+I R+T R IG+V  D+G DA++C VL  I +QSPDI QGV     + P+E GAGD G
Sbjct:    1 MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTSAWVDIEEITRETVREIGYVHSDMGFDANSCAVLNTIGKQSPDINQGVD---KEDPKEQGAGDQG 121

HSP 2 Score: 27.335 bits (59), Expect = 4.016e-36
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 3 
Query:  447 QGHMFGYATDETP 485
            QG MFGYAT+ETP
Sbjct:  120 QGIMFGYATNETP 132
[Close]
BLAST of FC876438 vs. ExPASy Swiss-Prot
Match: METK_VIBSL (S-adenosylmethionine synthetase OS=Vibrio splendidus (strain LGP32) GN=metK PE=3 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 6.815e-36
Identity = 74/124 (59.68%), Postives = 91/124 (73.39%), Query Frame = 2 
Query:   80 METFLFTSESVNEGHPDKLCDQISDAILDACLEQDPDSKVACETCTKTNLVMVFGEITTKANVNYEKIVRDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLSKRPEEIGAGDSG 451
            M   LFTSESV+EGHPDK+ DQISDA+LDA +EQDP ++VACET  KT +VMV GE+TT A V+ E+I R+T R IG+V  D+G DAD+C VL  I +QSPDI QGV       P++ GAGD G
Sbjct:    1 MAKHLFTSESVSEGHPDKIADQISDAVLDAIIEQDPKARVACETYVKTGMVMVGGEVTTSAWVDIEEITRETVREIGYVHSDMGFDADSCAVLNTIGKQSPDINQGVD---KADPKDQGAGDQG 121

HSP 2 Score: 27.335 bits (59), Expect = 6.815e-36
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 3 
Query:  447 QGHMFGYATDETP 485
            QG MFGYAT+ETP
Sbjct:  120 QGIMFGYATNETP 132
[Close]
BLAST of FC876438 vs. ExPASy Swiss-Prot
Match: METK_VIBFM (S-adenosylmethionine synthetase OS=Vibrio fischeri (strain MJ11) GN=metK PE=3 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 6.815e-36
Identity = 75/124 (60.48%), Postives = 91/124 (73.39%), Query Frame = 2 
Query:   80 METFLFTSESVNEGHPDKLCDQISDAILDACLEQDPDSKVACETCTKTNLVMVFGEITTKANVNYEKIVRDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLSKRPEEIGAGDSG 451
            M   LFTSESV+EGHPDK+ DQISDA+LDA LEQDP ++VACET  KT +VMV GE+TT A V+ E+I R+T R IG+V  D+G DA++C VL  I +QSPDI QGV       P+E GAGD G
Sbjct:    1 MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTSAWVDIEEITRETVREIGYVHSDMGFDANSCAVLNTIGKQSPDINQGVD---KADPKEQGAGDQG 121

HSP 2 Score: 27.335 bits (59), Expect = 6.815e-36
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 3 
Query:  447 QGHMFGYATDETP 485
            QG MFGYAT+ETP
Sbjct:  120 QGIMFGYATNETP 132
[Close]
BLAST of FC876438 vs. ExPASy Swiss-Prot
Match: METK_VIBF1 (S-adenosylmethionine synthetase OS=Vibrio fischeri (strain ATCC 700601 / ES114) GN=metK PE=3 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 6.815e-36
Identity = 75/124 (60.48%), Postives = 91/124 (73.39%), Query Frame = 2 
Query:   80 METFLFTSESVNEGHPDKLCDQISDAILDACLEQDPDSKVACETCTKTNLVMVFGEITTKANVNYEKIVRDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLSKRPEEIGAGDSG 451
            M   LFTSESV+EGHPDK+ DQISDA+LDA LEQDP ++VACET  KT +VMV GE+TT A V+ E+I R+T R IG+V  D+G DA++C VL  I +QSPDI QGV       P+E GAGD G
Sbjct:    1 MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTSAWVDIEEITRETVREIGYVHSDMGFDANSCAVLNTIGKQSPDINQGVD---KADPKEQGAGDQG 121

HSP 2 Score: 27.335 bits (59), Expect = 6.815e-36
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 3 
Query:  447 QGHMFGYATDETP 485
            QG MFGYAT+ETP
Sbjct:  120 QGIMFGYATNETP 132
The following BLAST results are available for this feature:
BLAST of FC876438 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK5_CAEEL8.214e-3759.50Probable S-adenosylmethionine synthetase 5 OS=Caen... [more]
METK_PROMH8.478e-3762.10S-adenosylmethionine synthetase OS=Proteus mirabil... [more]
METK_LACSS1.816e-3657.36S-adenosylmethionine synthetase OS=Lactobacillus s... [more]
METK_PROMT2.365e-3654.69S-adenosylmethionine synthetase OS=Prochlorococcus... [more]
METK_PHOLL2.418e-3660.48S-adenosylmethionine synthetase OS=Photorhabdus lu... [more]
METK_METFK3.083e-3659.68S-adenosylmethionine synthetase OS=Methylobacillus... [more]
METK_ALISL4.016e-3660.48S-adenosylmethionine synthetase OS=Aliivibrio salm... [more]
METK_VIBSL6.815e-3659.68S-adenosylmethionine synthetase OS=Vibrio splendid... [more]
METK_VIBFM6.815e-3660.48S-adenosylmethionine synthetase OS=Vibrio fischeri... [more]
METK_VIBF16.815e-3660.48S-adenosylmethionine synthetase OS=Vibrio fischeri... [more]

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Properties
Property NameValue
Genbank descriptionC32004G08EF DevOvary1 Citrus clementina cDNA clone C32004G08, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC876438 ID=FC876438; Name=FC876438; organism=Citrus clementina; type=EST; length=576bp
CCCCCCTCTATATTTCCTTTCTCGGGGGAGATTTCTCTCAATTCTAGATC
TAGCCGGTTCGCAGTTTTTGAAGTCCCCCATGGAGACTTTTTTGTTCACT
TCAGAATCTGTGAACGAGGGTCATCCAGACAAGCTCTGTGATCAGATCTC
CGATGCCATTCTCGATGCTTGTCTCGAACAAGACCCAGATAGCAAGGTGG
CTTGTGAAACCTGTACCAAGACTAACTTGGTTATGGTTTTTGGAGAGATC
ACAACCAAAGCTAATGTTAATTACGAGAAAATTGTTCGTGACACTTGCCG
CAACATTGGATTTGTCTCTGATGATGTGGGTTTGGATGCTGATAACTGTA
AGGTTTTGGTTTACATTGAGCAACAAAGCCCTGATATCGCCCAGGGCGTT
CATGGTCATTTGTCTAAACGCCCCGAGGAAATCGGTGCTGGTGATTCAGG
GGCATATGTTTGGTTATGCTACCGATGAGACTCCTGAATTTATTGCCTCT
GAGTCATGTTCTTGCCACCCAGCTTGGTGCTAAACTTACCGAGGTTCGCA
AGAATGGCACTTGCACTTGGATGAGG
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