FC876438 | Citrus Genome Database

Overview


Name	FC876438
Unique Name	FC876438
Type	EST
Organism	Citrus clementina (Clementine)
Sequence length	576

Libraries


Library Name	Type
DevOvary1	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Ccv1_Contig15105	contig	Ccv1_Contig15105:11..584.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of FC876438 vs. ExPASy Swiss-Prot
Match: METK_DICDI (S-adenosylmethionine synthetase OS=Dictyostelium discoideum GN=metK PE=1 SV=1)

HSP 1 Score: 170.244 bits (430), Expect = 6.882e-49
Identity = 83/125 (66.40%), Postives = 98/125 (78.40%), Query Frame = 2

Query:   80 METFLFTSESVNEGHPDKLCDQISDAILDACLEQDPDSKVACETCTKTNLVMVFGEITTKANVNYEKIVRDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLSK-RPEEIGAGDSG 451
            M ++LFTSESV EGHPDK+CDQ+SDA+LDACL QDP SKVACET TKT +VM+ GEITTKANV+Y+K+VR+  + IG+     G D   C VLV IEQQSPDIAQGV  H+ K  PE+IGAGD G
Sbjct:    1 MSSYLFTSESVTEGHPDKICDQVSDAVLDACLAQDPLSKVACETATKTGMVMILGEITTKANVDYQKVVREAVKKIGYDDSSKGFDYKTCNVLVAIEQQSPDIAQGV--HIGKVNPEDIGAGDQG 123

HSP 2 Score: 33.113 bits (74), Expect = 6.882e-49
Identity = 16/25 (64.00%), Postives = 18/25 (72.00%), Query Frame = 3

Query:  447 QGHMFGYATDETP------EFIASE 503
            QGHMFGYAT+ETP       F+ASE
Sbjct:  122 QGHMFGYATNETPTLMPLTHFLASE 146

HSP 3 Score: 32.7278 bits (73), Expect = 6.882e-49
Identity = 14/25 (56.00%), Postives = 18/25 (72.00%), Query Frame = 1

Query:  502 SHVLATQLGAKLTEVRKNGTCTWMR 576
            +H LA++L  KLTE+R NGT  W R
Sbjct:  140 THFLASELVNKLTELRHNGTLAWAR 164

[Close]

BLAST of FC876438 vs. ExPASy Swiss-Prot
Match: METK_ACACA (S-adenosylmethionine synthetase OS=Acanthamoeba castellanii GN=metK PE=2 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 9.552e-48
Identity = 76/123 (61.79%), Postives = 96/123 (78.05%), Query Frame = 2

Query:   83 ETFLFTSESVNEGHPDKLCDQISDAILDACLEQDPDSKVACETCTKTNLVMVFGEITTKANVNYEKIVRDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLSKRPEEIGAGDSG 451
            +T LFTSE V+E HPDKLCDQ+SDAILDACL  DP SKVACET +KT +VMVFGEITTK++ +Y+K++R+T + IGF    +G D   C +LV IEQQSPDIAQGV  H+ +  +++GAGD G
Sbjct:    5 KTLLFTSECVSESHPDKLCDQVSDAILDACLANDPYSKVACETASKTGMVMVFGEITTKSSFDYQKVIRETVKRIGFTDSSIGFDYKTCNILVAIEQQSPDIAQGV--HVGRSDDDLGAGDQG 125

HSP 2 Score: 34.2686 bits (77), Expect = 9.552e-48
Identity = 13/16 (81.25%), Postives = 16/16 (100.00%), Query Frame = 3

Query:  447 QGHMFGYATDETPEFI 494
            QGHMFG+AT+ETPEF+
Sbjct:  124 QGHMFGHATNETPEFM 139

HSP 3 Score: 33.8834 bits (76), Expect = 9.552e-48
Identity = 14/25 (56.00%), Postives = 19/25 (76.00%), Query Frame = 1

Query:  502 SHVLATQLGAKLTEVRKNGTCTWMR 576
            +HVLAT+L  ++TEVR N   +WMR
Sbjct:  142 THVLATRLCQRMTEVRNNSILSWMR 166

[Close]

BLAST of FC876438 vs. ExPASy Swiss-Prot
Match: METK2_YEAST (S-adenosylmethionine synthetase 2 OS=Saccharomyces cerevisiae GN=SAM2 PE=1 SV=3)

HSP 1 Score: 171.014 bits (432), Expect = 5.688e-45
Identity = 84/126 (66.67%), Postives = 98/126 (77.78%), Query Frame = 2

Query:   74 SPMETFLFTSESVNEGHPDKLCDQISDAILDACLEQDPDSKVACETCTKTNLVMVFGEITTKANVNYEKIVRDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLSKRPEEIGAGDSG 451
            S  +TFLFTSESV EGHPDK+CDQ+SDAILDACLEQDP SKVACET  KT ++MVFGEITTKA ++Y++IVRDT + IG+     G D   C VLV IEQQSPDIAQG+  H  K  E++GAGD G
Sbjct:    2 SKSKTFLFTSESVGEGHPDKICDQVSDAILDACLEQDPFSKVACETAAKTGMIMVFGEITTKARLDYQQIVRDTIKKIGYDDSAKGFDYKTCNVLVAIEQQSPDIAQGL--HYEKSLEDLGAGDQG 125

HSP 2 Score: 31.187 bits (69), Expect = 5.688e-45
Identity = 13/14 (92.86%), Postives = 13/14 (92.86%), Query Frame = 3

Query:  447 QGHMFGYATDETPE 488
            QG MFGYATDETPE
Sbjct:  124 QGIMFGYATDETPE 137

[Close]

BLAST of FC876438 vs. ExPASy Swiss-Prot
Match: METK1_YEAST (S-adenosylmethionine synthetase 1 OS=Saccharomyces cerevisiae GN=SAM1 PE=1 SV=2)

HSP 1 Score: 169.859 bits (429), Expect = 1.259e-44
Identity = 84/122 (68.85%), Postives = 95/122 (77.87%), Query Frame = 2

Query:   86 TFLFTSESVNEGHPDKLCDQISDAILDACLEQDPDSKVACETCTKTNLVMVFGEITTKANVNYEKIVRDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLSKRPEEIGAGDSG 451
            TFLFTSESV EGHPDK+CDQ+SDAILDACL +DP SKVACET  KT ++MVFGEITTKA ++Y+KIVRDT + IG+     G D   C VLV IEQQSPDIAQGVH    K  E+IGAGD G
Sbjct:    4 TFLFTSESVGEGHPDKICDQVSDAILDACLAEDPHSKVACETAAKTGMIMVFGEITTKAQLDYQKIVRDTIKKIGYDDSAKGFDYKTCNVLVAIEQQSPDIAQGVHE--EKDLEDIGAGDQG 123

HSP 2 Score: 31.187 bits (69), Expect = 1.259e-44
Identity = 13/14 (92.86%), Postives = 13/14 (92.86%), Query Frame = 3

Query:  447 QGHMFGYATDETPE 488
            QG MFGYATDETPE
Sbjct:  122 QGIMFGYATDETPE 135

[Close]

BLAST of FC876438 vs. ExPASy Swiss-Prot
Match: METK2_PONAB (S-adenosylmethionine synthetase isoform type-2 OS=Pongo abelii GN=MAT2A PE=2 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 1.503e-44
Identity = 76/122 (62.30%), Postives = 96/122 (78.69%), Query Frame = 2

Query:   86 TFLFTSESVNEGHPDKLCDQISDAILDACLEQDPDSKVACETCTKTNLVMVFGEITTKANVNYEKIVRDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLSKRPEEIGAGDSG 451
            TFLFTSESV EGHPDK+CDQISDA+LDA L+QDPD+KVACET  KT ++++ GEIT++A V+Y+K+VR+  ++IG+     G D   C VLV +EQQSPDIAQGV  HL +  E+IGAGD G
Sbjct:   17 TFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAVDYQKVVREAVKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGV--HLDRNEEDIGAGDQG 136

HSP 2 Score: 33.113 bits (74), Expect = 1.503e-44
Identity = 14/23 (60.87%), Postives = 18/23 (78.26%), Query Frame = 1

Query:  508 VLATQLGAKLTEVRKNGTCTWMR 576
            VLA +L AKL E+R+NGT  W+R
Sbjct:  155 VLAHKLNAKLAELRRNGTLPWLR 177

HSP 3 Score: 28.1054 bits (61), Expect = 1.503e-44
Identity = 12/14 (85.71%), Postives = 12/14 (85.71%), Query Frame = 3

Query:  447 QGHMFGYATDETPE 488
            QG MFGYATDET E
Sbjct:  135 QGLMFGYATDETEE 148

[Close]

BLAST of FC876438 vs. ExPASy Swiss-Prot
Match: METK2_MACFA (S-adenosylmethionine synthetase isoform type-2 OS=Macaca fascicularis GN=MAT2A PE=2 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 1.503e-44
Identity = 76/122 (62.30%), Postives = 96/122 (78.69%), Query Frame = 2

Query:   86 TFLFTSESVNEGHPDKLCDQISDAILDACLEQDPDSKVACETCTKTNLVMVFGEITTKANVNYEKIVRDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLSKRPEEIGAGDSG 451
            TFLFTSESV EGHPDK+CDQISDA+LDA L+QDPD+KVACET  KT ++++ GEIT++A V+Y+K+VR+  ++IG+     G D   C VLV +EQQSPDIAQGV  HL +  E+IGAGD G
Sbjct:   17 TFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAVDYQKVVREAVKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGV--HLDRNEEDIGAGDQG 136

HSP 2 Score: 33.113 bits (74), Expect = 1.503e-44
Identity = 14/23 (60.87%), Postives = 18/23 (78.26%), Query Frame = 1

Query:  508 VLATQLGAKLTEVRKNGTCTWMR 576
            VLA +L AKL E+R+NGT  W+R
Sbjct:  155 VLAHKLNAKLAELRRNGTLPWLR 177

HSP 3 Score: 28.1054 bits (61), Expect = 1.503e-44
Identity = 12/14 (85.71%), Postives = 12/14 (85.71%), Query Frame = 3

Query:  447 QGHMFGYATDETPE 488
            QG MFGYATDET E
Sbjct:  135 QGLMFGYATDETEE 148

[Close]

BLAST of FC876438 vs. ExPASy Swiss-Prot
Match: METK2_HUMAN (S-adenosylmethionine synthetase isoform type-2 OS=Homo sapiens GN=MAT2A PE=1 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 1.503e-44
Identity = 76/122 (62.30%), Postives = 96/122 (78.69%), Query Frame = 2

Query:   86 TFLFTSESVNEGHPDKLCDQISDAILDACLEQDPDSKVACETCTKTNLVMVFGEITTKANVNYEKIVRDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLSKRPEEIGAGDSG 451
            TFLFTSESV EGHPDK+CDQISDA+LDA L+QDPD+KVACET  KT ++++ GEIT++A V+Y+K+VR+  ++IG+     G D   C VLV +EQQSPDIAQGV  HL +  E+IGAGD G
Sbjct:   17 TFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAVDYQKVVREAVKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGV--HLDRNEEDIGAGDQG 136

HSP 2 Score: 33.113 bits (74), Expect = 1.503e-44
Identity = 14/23 (60.87%), Postives = 18/23 (78.26%), Query Frame = 1

Query:  508 VLATQLGAKLTEVRKNGTCTWMR 576
            VLA +L AKL E+R+NGT  W+R
Sbjct:  155 VLAHKLNAKLAELRRNGTLPWLR 177

HSP 3 Score: 28.1054 bits (61), Expect = 1.503e-44
Identity = 12/14 (85.71%), Postives = 12/14 (85.71%), Query Frame = 3

Query:  447 QGHMFGYATDETPE 488
            QG MFGYATDET E
Sbjct:  135 QGLMFGYATDETEE 148

[Close]

BLAST of FC876438 vs. ExPASy Swiss-Prot
Match: METK2_MOUSE (S-adenosylmethionine synthetase isoform type-2 OS=Mus musculus GN=Mat2a PE=2 SV=2)

HSP 1 Score: 159.844 bits (403), Expect = 1.955e-44
Identity = 75/122 (61.48%), Postives = 96/122 (78.69%), Query Frame = 2

Query:   86 TFLFTSESVNEGHPDKLCDQISDAILDACLEQDPDSKVACETCTKTNLVMVFGEITTKANVNYEKIVRDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLSKRPEEIGAGDSG 451
            TFLFTSESV EGHPDK+CDQISDA+LDA L+QDPD+KVACET  KT ++++ GEIT++A ++Y+K+VR+  ++IG+     G D   C VLV +EQQSPDIAQGV  HL +  E+IGAGD G
Sbjct:   17 TFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAIDYQKVVREAIKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGV--HLDRNEEDIGAGDQG 136

HSP 2 Score: 33.113 bits (74), Expect = 1.955e-44
Identity = 14/23 (60.87%), Postives = 18/23 (78.26%), Query Frame = 1

Query:  508 VLATQLGAKLTEVRKNGTCTWMR 576
            VLA +L AKL E+R+NGT  W+R
Sbjct:  155 VLAHKLNAKLAELRRNGTLPWLR 177

HSP 3 Score: 28.1054 bits (61), Expect = 1.955e-44
Identity = 12/14 (85.71%), Postives = 12/14 (85.71%), Query Frame = 3

Query:  447 QGHMFGYATDETPE 488
            QG MFGYATDET E
Sbjct:  135 QGLMFGYATDETEE 148

[Close]

BLAST of FC876438 vs. ExPASy Swiss-Prot
Match: METK_SCHPO (S-adenosylmethionine synthetase OS=Schizosaccharomyces pombe GN=sam1 PE=2 SV=1)

HSP 1 Score: 168.703 bits (426), Expect = 2.786e-44
Identity = 80/123 (65.04%), Postives = 99/123 (80.49%), Query Frame = 2

Query:   83 ETFLFTSESVNEGHPDKLCDQISDAILDACLEQDPDSKVACETCTKTNLVMVFGEITTKANVNYEKIVRDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLSKRPEEIGAGDSG 451
            +TFLFTSESV EGHPDK+CDQISDAILDACL+ DP SKVACET +KT +VMVFGEITT++ ++Y+K++R+T ++IG+   + G D   C VLV IEQQSPDIAQG+  H  K  EE+GAGD G
Sbjct:    3 QTFLFTSESVGEGHPDKICDQISDAILDACLKDDPFSKVACETASKTGMVMVFGEITTRSQIDYQKVIRNTIKSIGYDDSEKGFDYKTCNVLVAIEQQSPDIAQGL--HYEKALEELGAGDQG 123

HSP 2 Score: 31.187 bits (69), Expect = 2.786e-44
Identity = 13/14 (92.86%), Postives = 13/14 (92.86%), Query Frame = 3

Query:  447 QGHMFGYATDETPE 488
            QG MFGYATDETPE
Sbjct:  122 QGIMFGYATDETPE 135

[Close]

BLAST of FC876438 vs. ExPASy Swiss-Prot
Match: METK2_RAT (S-adenosylmethionine synthetase isoform type-2 OS=Rattus norvegicus GN=Mat2a PE=2 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 4.300e-44
Identity = 74/122 (60.66%), Postives = 96/122 (78.69%), Query Frame = 2

Query:   86 TFLFTSESVNEGHPDKLCDQISDAILDACLEQDPDSKVACETCTKTNLVMVFGEITTKANVNYEKIVRDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLSKRPEEIGAGDSG 451
            TFLFTSESV EGHPDK+CDQI+DA+LDA L+QDPD+KVACET  KT ++++ GEIT++A ++Y+K+VR+  ++IG+     G D   C VLV +EQQSPDIAQGV  HL +  E+IGAGD G
Sbjct:   17 TFLFTSESVGEGHPDKICDQINDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAIDYQKVVREAIKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGV--HLDRNEEDIGAGDQG 136

HSP 2 Score: 33.113 bits (74), Expect = 4.300e-44
Identity = 14/23 (60.87%), Postives = 18/23 (78.26%), Query Frame = 1

Query:  508 VLATQLGAKLTEVRKNGTCTWMR 576
            VLA +L AKL E+R+NGT  W+R
Sbjct:  155 VLAHKLNAKLAELRRNGTLPWLR 177

HSP 3 Score: 28.1054 bits (61), Expect = 4.300e-44
Identity = 12/14 (85.71%), Postives = 12/14 (85.71%), Query Frame = 3

Query:  447 QGHMFGYATDETPE 488
            QG MFGYATDET E
Sbjct:  135 QGLMFGYATDETEE 148

The following BLAST results are available for this feature:

BLAST of FC876438 vs. ExPASy Swiss-Prot

BLAST of FC876438 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
METK_DICDI	6.882e-49	66.40	S-adenosylmethionine synthetase OS=Dictyostelium d...	[more]
METK_ACACA	9.552e-48	61.79	S-adenosylmethionine synthetase OS=Acanthamoeba ca...	[more]
METK2_YEAST	5.688e-45	66.67	S-adenosylmethionine synthetase 2 OS=Saccharomyces...	[more]
METK1_YEAST	1.259e-44	68.85	S-adenosylmethionine synthetase 1 OS=Saccharomyces...	[more]
METK2_PONAB	1.503e-44	62.30	S-adenosylmethionine synthetase isoform type-2 OS=...	[more]
METK2_MACFA	1.503e-44	62.30	S-adenosylmethionine synthetase isoform type-2 OS=...	[more]
METK2_HUMAN	1.503e-44	62.30	S-adenosylmethionine synthetase isoform type-2 OS=...	[more]
METK2_MOUSE	1.955e-44	61.48	S-adenosylmethionine synthetase isoform type-2 OS=...	[more]
METK_SCHPO	2.786e-44	65.04	S-adenosylmethionine synthetase OS=Schizosaccharom...	[more]
METK2_RAT	4.300e-44	60.66	S-adenosylmethionine synthetase isoform type-2 OS=...	[more]

Pages

Properties


Property Name	Value
Genbank description	C32004G08EF DevOvary1 Citrus clementina cDNA clone C32004G08, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FC876438 ID=FC876438; Name=FC876438; organism=Citrus clementina; type=EST; length=576bp
CCCCCCTCTATATTTCCTTTCTCGGGGGAGATTTCTCTCAATTCTAGATC
TAGCCGGTTCGCAGTTTTTGAAGTCCCCCATGGAGACTTTTTTGTTCACT
TCAGAATCTGTGAACGAGGGTCATCCAGACAAGCTCTGTGATCAGATCTC
CGATGCCATTCTCGATGCTTGTCTCGAACAAGACCCAGATAGCAAGGTGG
CTTGTGAAACCTGTACCAAGACTAACTTGGTTATGGTTTTTGGAGAGATC
ACAACCAAAGCTAATGTTAATTACGAGAAAATTGTTCGTGACACTTGCCG
CAACATTGGATTTGTCTCTGATGATGTGGGTTTGGATGCTGATAACTGTA
AGGTTTTGGTTTACATTGAGCAACAAAGCCCTGATATCGCCCAGGGCGTT
CATGGTCATTTGTCTAAACGCCCCGAGGAAATCGGTGCTGGTGATTCAGG
GGCATATGTTTGGTTATGCTACCGATGAGACTCCTGAATTTATTGCCTCT
GAGTCATGTTCTTGCCACCCAGCTTGGTGCTAAACTTACCGAGGTTCGCA
AGAATGGCACTTGCACTTGGATGAGG