FC878296

Overview
NameFC878296
Unique NameFC878296
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length862
Libraries
Library NameType
IVIA1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC878296 vs. ExPASy Swiss-Prot
Match: PME6_ARATH (Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana GN=PME6 PE=2 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 3.303e-53
Identity = 121/281 (43.06%), Postives = 172/281 (61.21%), Query Frame = 1
Query:   94 IFSLNFAHGELITSCSQTPYPEICNHYTATNFLSNLELDQTQFSFRDLNLKVTIDQAIHAHKLISTMDLS-SFNKLAKLALVDCKDLYDDTVNHLNRSMSS----SNPIDSVTWLSAAIANQETCKNGFTDFNL-HSHLQSLPFM-SGNFSKLLSNSLAITKSTVSSSSIPYAYKRNG---------------GRRLLV---NGFPTWVSAADRRLLQSSGVGPKADVVVAQDGSGNYKTISEGVAAAVKLGGGSKRVVIYVKRGVYRENVEIKRSMKNLM 861
            + S++  +   ITSC QTPYP +C+H+ + + L  L+     F+F DL +  T+DQA+  H+L+S++    S +K A  AL DC +LY+DT++ LN S  S    S+P D  T LSAAIANQ+TC+NGF DF L  S+ +  P     N +K +SNSLA+TK+   + ++   Y   G                RRLL+     FP+W   +DR+LL+ S    KAD+VVA+DGSG+Y +I + V AA KL   ++R+VIYVK GVYRENV IK+S+KN+M
Sbjct:   24 VVSISHLNAHFITSCKQTPYPSVCDHHMSNSPLKTLDDQTDGFTFHDLVVSSTMDQAVQLHRLVSSLKQHHSLHKHATSALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTSLSAAIANQDTCRNGFRDFKLTSSYSKYFPVQFHRNLTKSISNSLAVTKAAAEAEAVAEKYPSTGFTKFSKQRSSAGGGSHRRLLLFSDEKFPSWFPLSDRKLLEDSKTTAKADLVVAKDGSGHYTSIQQAVNAAAKLPRRNQRLVIYVKAGVYRENVVIKKSIKNVM 304          
BLAST of FC878296 vs. ExPASy Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 2.298e-38
Identity = 101/276 (36.59%), Postives = 161/276 (58.33%), Query Frame = 1
Query:   70 IQLFIHFLIFSLN-----FAHGELITSCSQTPYPEICNHY-TATNFLSNLELDQTQFSFRDLNLKVTIDQAIHAHKLISTMDLSSFNKLAKLALVDCKDLYDDTVNHLNRSMSSS---NPIDSVTWLSAAIANQETCKNGFTDFNLHSHLQSLPFMSGNFSKLLSNSLAITKSTVSSSSIPY---AYKRNGGRRLLVNGFPTWVSAADRRLLQSSGVGPKADVVVAQDGSGNYKTISEGVAAAVKLGGGSKRVVIYVKRGVYRENVEIKRSMKNLM 861
            I + +  ++FSL+     ++  E+ + C +TP P+ C ++ T    +++++ D     F  ++L++ +++A  A     T+     N+  K A  DC++LY+ TV  LN++ +SS     +D  TWLS A+ N ETC+    D  +  ++  LP +S N +KL+SN+L++ K       +PY   +YK         +GFPTWV   DR+LLQ++   P+A++VVAQDGSGN KTI E VAAA + GG   R VIY+K G Y EN+E+K  +KN+M
Sbjct:    5 ILITVSLVLFSLSHTSFGYSPEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQDT---DFYKISLQLALERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQTWLSTALTNLETCRASLEDLGVPEYV--LPLLSNNVTKLISNTLSLNK-------VPYNEPSYK---------DGFPTWVKPGDRKLLQTT---PRANIVVAQDGSGNVKTIQEAVAAASRAGG--SRYVIYIKAGTYNENIEVK--LKNIM 252          
BLAST of FC878296 vs. ExPASy Swiss-Prot
Match: PME17_ARATH (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=PME17 PE=2 SV=2)

HSP 1 Score: 156.762 bits (395), Expect = 1.490e-37
Identity = 101/245 (41.22%), Postives = 143/245 (58.37%), Query Frame = 1
Query:  136 CSQTPYPEICNHYTATNFLSNLELDQTQFSFRDLNLKVTIDQAIHAHKLISTMDLSSFNKLAKLALVDCKDLYDDTVNHLNRSMSSS---NPIDSVTWLSAAIANQETCKNGFTDFNLHSHLQSLPFMSGNFSKLLSNSLAITKSTVSSSSIPYAYKRNGGRRLLVNGFPTWVSAADRRLLQSSGVGPKADVVVAQDGSGNYKTISEGVAAAVKLGGGSKRVVIYVKRGVYRENVEIKRSMKNLM 861
            CSQTP P+ C ++   N  SN E  +++  F  +++K+ +D+AI A     T+     +   K A  DC  LYD TV+ +N +M  +   + +D+ TWLS A+ N +TC+ GF +  +   +  LP MS N S LL N+LAI K       +P+ Y          +GFP+WV   DR+LLQSS   PK + VVA+DGSGN+KTI E + AA     GS R VIYVK+GVY EN+EI++  KN+M
Sbjct:   34 CSQTPNPKPCEYFLTHN--SNNEPIKSESEFLKISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLELGVTDIV--LPLMSNNVSNLLCNTLAINK-------VPFNYTPPEK-----DGFPSWVKPGDRKLLQSST--PKDNAVVAKDGSGNFKTIKEAIDAA----SGSGRFVIYVKQGVYSENLEIRK--KNVM 254          
BLAST of FC878296 vs. ExPASy Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 3.214e-32
Identity = 95/270 (35.19%), Postives = 155/270 (57.41%), Query Frame = 1
Query:  115 HGELITSCSQTPYPEICNHYTATNFLSNLELDQTQFSFRDLNLKVTIDQAIHAH----KLISTMDLSSFNKLAKLALVDCKDLYDDTVNHLNRSMS-----------SSNPIDSVTWLSAAIANQETCKNGFTDFNLHSHLQ-SLPFMSGNFSKLLSNSLAITKSTVSSSSIPYAYKRNGGRRLL-----VNGFPTWVSAADRRLLQSSGVGPKADVVVAQDGSGNYKTISEGVAAAVKLGGGSKRVVIYVKRGVYRENVEIKRSMKNLM 861
            H  L +SCS T YP++C    A    ++ ++  +Q    +++L +T     H +    KL+   +L+   K  K+AL DC +  D+T++ L++++            S +  D  T +SAA+ NQ TC +GF+  + + H++ +L     +  K+ SN+LA+ K+   +  +    + +  R+L+     V+G+P W+S  DRRLLQSS V P  +VVVA DGSGN+KT++  VAAA +  GG+KR +I +K GVYRENVE+ +  KN+M
Sbjct:   66 HAILKSSCSSTRYPDLCFSAIAAVPEASKKVT-SQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLT---KREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMM--IMRTSNNRKLIEETSTVDGWPAWLSTGDRRLLQSSSVTP--NVVVAADGSGNFKTVAASVAAAPQ--GGTKRYIIRIKAGVYRENVEVTKKHKNIM 325          
BLAST of FC878296 vs. ExPASy Swiss-Prot
Match: PME60_ARATH (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana GN=PME60 PE=2 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 7.160e-32
Identity = 91/253 (35.97%), Postives = 137/253 (54.15%), Query Frame = 1
Query:  136 CSQTPYPEICNHYTATNFLSNLELDQTQFSFRDLNLKVTIDQAIHAHKLI--STMDLSSFNKLAKLALVDCKDLYDDTVNHLNRSMSSSNP--------IDSVTWLSAAIANQETCKNGFTDFNLHSHLQSLPFMSGN-FSKLLSNSLAITKSTVSSSSIPYAYKRNGGRRLLVNGFPTWVSAADRRLLQSSGVGPKADVVVAQDGSGNYKTISEGVAAAVKLGGGSKRVVIYVKRGVYRENVEIKRSMKNLM 861
            C +TPYP  C  Y   +  S   L      FR L ++  +D+A+ A   +  S+ + + F K A LA  DC +LY DTV  LNR++   +          D+ TWLS A+ N ETC+ G +D N+     + P +S    S L+SN LA+  + +++        +N        GFPTWVS  +RRLLQ   V  +A++VVA+DGSG++KT+   +  A +    S R VIYVKRG+Y+EN+ ++ +  N+M
Sbjct:   42 CDKTPYPYPCKRYFIKH--SGFRLPTQISEFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLA--DCINLYGDTVMQLNRTLQGVSSKTGRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDF--TTPIVSNTKISHLISNCLAVNGALLTAG-------KNDSTTGDSKGFPTWVSRKERRLLQLQSV--RANLVVAKDGSGHFKTVQAAIDVAGRRKVTSGRFVIYVKRGIYQENLNVRLNNDNIM 279          
BLAST of FC878296 vs. ExPASy Swiss-Prot
Match: PME59_ARATH (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana GN=PME59 PE=2 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 1.221e-31
Identity = 92/289 (31.83%), Postives = 149/289 (51.56%), Query Frame = 1
Query:   52 MDKLVLIQLFIHFLIFSLNFAHGELITS-------CSQTPYPEICNHYTATNFLSNLELDQTQFS-FRDLNLKVTIDQAIHAHKLISTMDLSSFNKLAKLALVDCKDLYDDTVNHLNRSMSSSNP----------IDSVTWLSAAIANQETCKNGFTDFNLHSHLQSLPFMSGN-FSKLLSNSLAITKSTVSSSSIPYAYKRNGGRRLLVNGFPTWVSAADRRLLQSSGVGPKADVVVAQDGSGNYKTISEGVAAAVKLGGGSKRVVIYVKRGVYRENVEIKRSMKNLM 861
            M KL +  LF+H ++  L   H     +       C +TPYP+ C  Y   +   N     TQ S FR + ++  +D+AI A   ++    +  +   +  L DC DLY DT+  LNR++   +P           D+ TWLS A+ N ETC+ G +D N+   +   P +S    S L+SN LA+  + +++ +        G       GFPTW+S  D+RLL++     +A++VVA+DGSG++ T+   +  A +    S R VIYVKRG+Y+EN+ ++ +  ++M
Sbjct:    5 MQKLSI--LFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNH---NGFQQPTQLSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFIT--PIVSNTKISHLISNCLAVNGALLTAGN-------KGNTTANQKGFPTWLSRKDKRLLRAV----RANLVVAKDGSGHFNTVQAAIDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIM 275          
BLAST of FC878296 vs. ExPASy Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 1.221e-31
Identity = 95/270 (35.19%), Postives = 153/270 (56.67%), Query Frame = 1
Query:  115 HGELITSCSQTPYPEICNHYTATNFLSNLELDQTQFSFRDLNLKVTIDQAIHAH----KLISTMDLSSFNKLAKLALVDCKDLYDDTVNHLNRSMS-----------SSNPIDSVTWLSAAIANQETCKNGFTDFNLHSHLQ-SLPFMSGNFSKLLSNSLAITKSTVSSSSIPYAYKRNGGRRLL-----VNGFPTWVSAADRRLLQSSGVGPKADVVVAQDGSGNYKTISEGVAAAVKLGGGSKRVVIYVKRGVYRENVEIKRSMKNLM 861
            H  L +SCS T YP++C    A    ++ ++  +Q    +++L +T     H +    KL+   +L+   K  K+AL DC +  D+T++ L++++            S +  D  T +SAA+ NQ TC +GF+  + + H++ +L     +  K+ SN+LA+ K+   +  +    + +  R+L      V+G+P W+S  DRRLLQSS V P A  VVA DGSGN+KT++  VAAA +  GG+KR +I +K GVYRENVE+ +  KN+M
Sbjct:   66 HAILKSSCSSTRYPDLCFSAIAAVPEASKKVT-SQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLT---KREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMM--IMRTSNNRKLTEETSTVDGWPAWLSPGDRRLLQSSSVTPNA--VVAADGSGNFKTVAAAVAAAPQ--GGTKRYIIRIKAGVYRENVEVTKKHKNIM 325          
BLAST of FC878296 vs. ExPASy Swiss-Prot
Match: PME33_ARATH (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana GN=PME33 PE=2 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 8.753e-30
Identity = 87/262 (33.21%), Postives = 133/262 (50.76%), Query Frame = 1
Query:  118 GELITSCSQTPYPEICNHYTATNFLSNLELDQTQF--SFRDLNLKVTIDQAIHAHKLISTMDLSSFNKLAKLALVDCKDLYDDTVNHLNRSMSSSNP----------IDSVTWLSAAIANQETCKNGFTDFNLHSHLQSLPFMSG-NFSKLLSNSLAITKSTVSSSSIPYAYKRNGGRRLLVNGFPTWVSAADRRLLQSSGVGPKA-DVVVAQDGSGNYKTISEGVAAAVKLGGGSKRVVIYVKRGVYRENVEIKRSMKNLM 861
            G++   C++TP+ E CN+Y   +  +N+ L   +F   F  + +KV +DQA+  H        S  N   K A  DC +L+ +TV  LNR++   NP           D+ TWLS A  N ETC++G  D N+   +  +P +S  N S L+ N LA+    +                     FP+WVS  +RRLL S+ +   +  +VVAQD SG++++I   +  A +    S R VIYVK+GVYREN+++     N+M
Sbjct:   82 GDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCRSGSEDLNVSDFV--MPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHK-------EYFPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKS-RFVIYVKKGVYRENIDVGNDNHNIM 333          
BLAST of FC878296 vs. ExPASy Swiss-Prot
Match: PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)

HSP 1 Score: 124.02 bits (310), Expect = 1.070e-27
Identity = 92/287 (32.06%), Postives = 138/287 (48.08%), Query Frame = 1
Query:  112 AHGELITSCSQTPYPEICNHYTATNFLSNLELDQTQFSFRDLNLKVTIDQAIHAHKLISTM--DLSSFNKLAKLALVDCKDLYDDTVNHLNRSMSS-----------SNPIDSVTWLSAAIANQETCKNGFTDFNLHSHLQSLPFMSGNF--SKLLSNSLAITKSTVSSSSIPYAYKRN----------------------GGRRLLVNGFPTWVSAADRRLLQSSGVGPKADVVVAQDGSGNYKTISEGVAAAVKLGGGSKRVVIYVKRGVYRENVEIKRSMKNLM 861
            +H  L +SCS T YPE+C     T     +EL  +Q    + ++ +TI    H +  +  +           K AL DC +  D+T++ L+ ++              +  D  T +S+AI NQETC +GF+  +    ++    + G      + SN+LA+ K+   +    +  K                        G   L   G+PTW+SA DRRLLQ SGV  KAD  VA DGSG +KT++  VAAA +    +KR VI++K GVYRENVE+ +  KN+M
Sbjct:   55 SHAVLRSSCSSTRYPELCISAVVT--AGGVELT-SQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKA-LLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIAGAGELDSEGWPTWLSAGDRRLLQGSGV--KADATVAADGSGTFKTVAAAVAAAPE--NSNKRYVIHIKAGVYRENVEVAKKKKNIM 333          
BLAST of FC878296 vs. ExPASy Swiss-Prot
Match: PMEU1_SOLLC (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 9.058e-27
Identity = 92/270 (34.07%), Postives = 142/270 (52.59%), Query Frame = 1
Query:  112 AHGELITSCSQTPYPEICNHYTATNFLSNLELDQT-QFSFRDLNLKVTIDQAIHAHKLISTM--DLSSFNKLAKLALVDCKDLYDDTVNHL-----------NRSMSSSNPIDSVTWLSAAIANQETCKNGFTDFNLHSHLQSLPFMSG--NFSKLLSNSLAIT---KSTVSSSSIPYAYKRNGGRRLLVNG-FPTWVSAADRRLLQSSGVGPKADVVVAQDGSGNYKTISEGVAAAVKLGGGSKRVVIYVKRGVYRENVEIKRSMKNLM 861
            AH  + ++CS T +PE+C  Y+A   +S+     T Q    +L+L +T+      +  +  +           K+AL DC +  D+T++ L           N+     +  D  T +S+AI NQETC +GF+       ++ +  + G  +  K+ SN+LA+      T  ++ +  +   N  + +  NG +P W+SA DRRLLQSS V P  DVVVA DGSG+YKT+SE V  A +    SKR VI +K GVYRENV++ +   N+M
Sbjct:   62 AHAIVKSACSNTLHPELC--YSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKVALHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQETCLDGFSHDEADKKVRKV-LLKGQKHVEKMCSNALAMICNMTDTDIANEMKLSAPANNRKLVEDNGEWPEWLSAGDRRLLQSSTVTP--DVVVAADGSGDYKTVSEAVRKAPEKS--SKRYVIRIKAGVYRENVDVPKKKTNIM 324          
The following BLAST results are available for this feature:
BLAST of FC878296 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 44
Match NameE-valueIdentityDescription
PME6_ARATH3.303e-5343.06Probable pectinesterase/pectinesterase inhibitor 6... [more]
PME2_CITSI2.298e-3836.59Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE... [more]
PME17_ARATH1.490e-3741.22Probable pectinesterase/pectinesterase inhibitor 1... [more]
PME3_CITSI3.214e-3235.19Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1[more]
PME60_ARATH7.160e-3235.97Probable pectinesterase/pectinesterase inhibitor 6... [more]
PME59_ARATH1.221e-3131.83Probable pectinesterase/pectinesterase inhibitor 5... [more]
PME1_CITSI1.221e-3135.19Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE... [more]
PME33_ARATH8.753e-3033.21Probable pectinesterase/pectinesterase inhibitor 3... [more]
PME3_ARATH1.070e-2732.06Pectinesterase/pectinesterase inhibitor 3 OS=Arabi... [more]
PMEU1_SOLLC9.058e-2734.07Pectinesterase/pectinesterase inhibitor U1 OS=Sola... [more]

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Properties
Property NameValue
Genbank descriptionIC0AAA50AB11RM1 IVIA1 Citrus clementina cDNA clone IC0AAA50AB11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC878296 ID=FC878296; Name=FC878296; organism=Citrus clementina; type=EST; length=862bp
CATAATTCAATCATACCAAAACTCTATAGCTAGCAAGCCTTTTTGGTCTC
TATGGATAAGCTTGTGCTCATCCAACTTTTTATTCATTTTCTAATTTTTT
CTTTAAACTTTGCGCATGGTGAATTAATAACATCGTGTAGCCAGACCCCA
TATCCTGAAATATGCAACCACTACACGGCCACTAATTTTTTATCAAACTT
GGAGCTAGATCAAACCCAATTCTCTTTTCGTGACCTAAACCTTAAGGTCA
CCATTGACCAAGCCATACACGCTCACAAACTTATTTCGACTATGGACTTG
AGTTCATTCAATAAGCTAGCCAAGTTAGCTTTGGTCGATTGCAAGGATCT
TTACGACGATACAGTTAATCATCTCAATCGTTCGATGAGCTCTAGCAATC
CGATTGATTCTGTAACTTGGCTAAGTGCAGCTATTGCCAATCAAGAAACA
TGCAAAAACGGGTTCACTGATTTTAATTTGCATTCTCATCTACAATCCTT
GCCATTTATGTCAGGCAACTTCTCCAAGTTGCTTAGCAATTCACTGGCTA
TAACTAAGTCCACAGTTTCATCTTCATCAATACCGTATGCTTATAAAAGG
AACGGTGGCCGACGGTTACTTGTCAATGGATTTCCAACGTGGGTATCCGC
CGCTGATCGAAGACTTCTCCAGTCAAGTGGGGTGGGGCCAAAAGCTGATG
TTGTGGTGGCACAAGACGGGTCTGGTAATTACAAGACCATTTCAGAGGGT
GTGGCTGCAGCTGTCAAACTAGGAGGTGGAAGTAAAAGAGTTGTCATTTA
CGTGAAAAGGGGTGTGTATAGAGAGAATGTTGAGATTAAGAGATCTATGA
AGAACTTGATGT
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