FC920175

Overview
NameFC920175
Unique NameFC920175
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length687
Libraries
Library NameType
RVDevelop1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC920175 vs. ExPASy Swiss-Prot
Match: SERA_METTH (D-3-phosphoglycerate dehydrogenase OS=Methanobacterium thermoautotrophicum GN=serA PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 4.008e-18
Identity = 57/175 (32.57%), Postives = 98/175 (56.00%), Query Frame = 3
Query:  141 AIFSSAGAPVTAEILRLLPEVRLVVATSAGLNHIDVVECRRRGVALANAGNVFSEDVADYALGLLIDVLRKLSAADCFVRQGLWPINAEFPLGSKLGGKRVGIVGLGSIGSEVAKRLEAFGCCV----SYNSRN--KKPSVTYPFYPDVCELAANSDVLIICCALTDQTHHLINK 647
            AI   +   VT E++   P ++++     G++++DV     RG+ + NA    S  VA++++GL++ + RK++ AD  V++G W  N    +G +L GK +GI+G+G IGS+V  R +AFG  +     Y S+   ++  VT     D+  L   SD++ I   LT +T HLI++
Sbjct:   46 AIVVRSRTKVTREVIEAAPRLKIIARAGVGVDNVDVKAATDRGIMVINAPESTSITVAEHSIGLMLALARKIAIADRSVKEGKWEKNRF--MGIELNGKTLGIIGMGRIGSQVVVRTKAFGMDIMVYDPYISKEAAEEMGVT---VTDLETLLRESDIVTIHVPLTPETRHLISE 215          
BLAST of FC920175 vs. ExPASy Swiss-Prot
Match: GHRB_ERWT9 (Glyoxylate/hydroxypyruvate reductase B OS=Erwinia tasmaniensis (strain DSM 17950 / Et1/99) GN=ghrB PE=3 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.166e-17
Identity = 59/183 (32.24%), Postives = 95/183 (51.91%), Query Frame = 3
Query:  156 AGAPVTAEILRLLPEVRLVVATSAGLNHIDVVECRRRGVALANAGNVFSEDVADYALGLLIDVLRKLSAADCFVRQGLW--PINAEFPLGSKLGGKRVGIVGLGSIGSEVAKRLE-AFGCCVSYNSRNKKPSVTYPFYPDVCE---LAANSDVLIICCALTDQTHHLINKQSCWPWGKKELLL 686
            +G  V AE LR  P++R+  + S G ++ DV     RGV L +   V +E VAD  + L++   R++      V+ G W   I++++  G  +  K++GI+G+G IG  +A+R    FG  + YN+R         F  + C+   L A SD L I   LT+QTHHLI ++      +  +L+
Sbjct:   52 SGGKVDAEFLRQAPKLRVASSISVGYDNFDVAALNDRGVLLMHTPTVLTETVADTMMALVLSSARRVVEMAERVKSGEWRGSISSDW-FGIDVHHKKLGILGMGRIGLALAQRAHLGFGMPILYNARKHHDEAEQRFDAEYCDLDTLLAESDFLCISLPLTEQTHHLIGREQLAKMKRSAILI 233          
BLAST of FC920175 vs. ExPASy Swiss-Prot
Match: YGDH_SCHPO (Putative 2-hydroxyacid dehydrogenase C1773.17c OS=Schizosaccharomyces pombe GN=SPBC1773.17c PE=2 SV=3)

HSP 1 Score: 88.5817 bits (218), Expect = 3.393e-17
Identity = 62/170 (36.47%), Postives = 92/170 (54.12%), Query Frame = 3
Query:  150 SSAGAPVTAEILR-LLPEVRLVVATSAGLNHIDVVECRRRGVALANAGNVFSEDVADYALGLLIDVLRKLSAADCFVRQGLWPINAEFPLGSKLGGKRVGIVGLGSIGSEVAKRLEAFGCCVSYNSRNK----KPSVTYPFYPDVCELAANSDVLIICCALTDQTHHLIN 644
            ++A +P T E+L  LLP  +L V  +AG N++DV    R GV +AN  N  +E  A+  L L +  LR    A+  +R G W  N    L     GKRVGI+G+G+IG   A+++   GC + Y++RN+    +       +    EL ++SDV+ I C LT  TH LI+
Sbjct:   66 NAAISPFTEEMLGPLLPTCKLFVTGAAGYNNVDVDWATRNGVYVANTPNGPTEGTANMNLMLFMCTLRGAREAEQSLRLGKWRQN--LSLTDDPYGKRVGIIGMGAIGKSFAQKILPLGCEIVYHNRNRLEAEEEKRLGASFVSFDELLSSSDVISINCPLTPATHDLIS 233          
BLAST of FC920175 vs. ExPASy Swiss-Prot
Match: SERA_BACSU (D-3-phosphoglycerate dehydrogenase OS=Bacillus subtilis GN=serA PE=3 SV=3)

HSP 1 Score: 87.0409 bits (214), Expect = 9.872e-17
Identity = 54/181 (29.83%), Postives = 95/181 (52.49%), Query Frame = 3
Query:  129 HSVKAIFSSAGAPVTAEILRLLPEVRLVVATSAGLNHIDVVECRRRGVALANAGNVFSEDVADYALGLLIDVLRKLSAADCFVRQGLWPINAEFPLGSKLGGKRVGIVGLGSIGSEVAKRLEAFGCCVSY------NSRNKKPSVTYPFYPDVCELAANSDVLIICCALTDQTHHLINKQS 653
            H+  A+   +   VT ++   +  +++V     G+++ID+ E  + GV + NA N  +   A++   ++  ++R +  A+  V+   W  N    +GS+L GK +GIVGLG IGSE+A+R  AFG  V          R KK  V    + +V E   ++D++ +   LT +T  L+NK++
Sbjct:   39 HTFDALLVRSATKVTEDLFNKMTSLKIVGRAGVGVDNIDIDEATKHGVIVINAPNGNTISTAEHTFAMISSLMRHIPQANISVKSREW--NRTAYVGSELYGKTLGIVGLGRIGSEIAQRARAFGMTVHVFDPFLTEERAKKIGVNSRTFEEVLE---SADIITVHTPLTKETKGLLNKET 214          
BLAST of FC920175 vs. ExPASy Swiss-Prot
Match: TKRA_BACSU (Probable 2-ketogluconate reductase OS=Bacillus subtilis GN=yvcT PE=3 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.425e-15
Identity = 48/171 (28.07%), Postives = 90/171 (52.63%), Query Frame = 3
Query:  153 SAGAPVTAEILRLLPEVRLVVATSAGLNHIDVVECRRRGVALANAGNVFSEDVADYALGLLIDVLRKLSAADCFVRQGLW-PINAEFPLGSKLGGKRVGIVGLGSIGSEVAKRLE-AFGCCVSYNSRNKKPSVTYPF---YPDVCELAANSDVLIICCALTDQTHHLINKQ 650
            ++G  +  E+L   P++++V   S G ++ D+   + RGV   +      + VAD A  L++   R+++  D FVR G W  +  E   G  +  + +GI+G+G IG + A+R +  F   V Y++R++K          Y ++  L   SD +++   LTD+T+H+I ++
Sbjct:   54 TSGPSINRELLEHAPKLKVVSNQSVGYDNFDIEAMKERGVVGTHTPYTLDDTVADLAFSLILSSARRVAELDRFVRAGKWGTVEEEALFGIDVHHQTLGIIGMGRIGEQAARRAKFGFDMEVLYHNRHRKQETEDSIGVKYAELDTLLEQSDFILLITPLTDETYHMIGER 224          
BLAST of FC920175 vs. ExPASy Swiss-Prot
Match: SERA_MYCTU (D-3-phosphoglycerate dehydrogenase OS=Mycobacterium tuberculosis GN=serA PE=1 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.425e-15
Identity = 55/174 (31.61%), Postives = 91/174 (52.30%), Query Frame = 3
Query:  141 AIFSSAGAPVTAEILRLLPEVRLVVATSAGLNHIDVVECRRRGVALANAGNVFSEDVADYALGLLIDVLRKLSAADCFVRQGLWPINAEFPLGSKLGGKRVGIVGLGSIGSEVAKRLEAFGCCVSYNSRNKKPSVTYPFYPDVC---ELAANSDVLIICCALTDQTHHLINKQS 653
            A+   +   V AE+L   P++++V     GL+++DV     RGV + NA        A++AL LL+   R++ AAD  +R+  W   + F  G+++ GK VG+VGLG IG  VA+R+ AFG  V        P+       ++    +L A +D + +    T +T  LI+K++
Sbjct:   46 ALLVRSATTVDAEVLAAAPKLKIVARAGVGLDNVDVDAATARGVLVVNAPTSNIHSAAEHALALLLAASRQIPAADASLREHTWK-RSSFS-GTEIFGKTVGVVGLGRIGQLVAQRIAAFGAYVVAYDPYVSPARAAQLGIELLSLDDLLARADFISVHLPKTPETAGLIDKEA 217          
BLAST of FC920175 vs. ExPASy Swiss-Prot
Match: SERA_MYCBO (D-3-phosphoglycerate dehydrogenase OS=Mycobacterium bovis GN=serA PE=3 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.425e-15
Identity = 55/174 (31.61%), Postives = 91/174 (52.30%), Query Frame = 3
Query:  141 AIFSSAGAPVTAEILRLLPEVRLVVATSAGLNHIDVVECRRRGVALANAGNVFSEDVADYALGLLIDVLRKLSAADCFVRQGLWPINAEFPLGSKLGGKRVGIVGLGSIGSEVAKRLEAFGCCVSYNSRNKKPSVTYPFYPDVC---ELAANSDVLIICCALTDQTHHLINKQS 653
            A+   +   V AE+L   P++++V     GL+++DV     RGV + NA        A++AL LL+   R++ AAD  +R+  W   + F  G+++ GK VG+VGLG IG  VA+R+ AFG  V        P+       ++    +L A +D + +    T +T  LI+K++
Sbjct:   46 ALLVRSATTVDAEVLAAAPKLKIVARAGVGLDNVDVDAATARGVLVVNAPTSNIHSAAEHALALLLAASRQIPAADASLREHTWK-RSSFS-GTEIFGKTVGVVGLGRIGQLVAQRIAAFGAYVVAYDPYVSPARAAQLGIELLSLDDLLARADFISVHLPKTPETAGLIDKEA 217          
BLAST of FC920175 vs. ExPASy Swiss-Prot
Match: SERA_METJA (D-3-phosphoglycerate dehydrogenase OS=Methanocaldococcus jannaschii GN=serA PE=3 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.862e-15
Identity = 46/169 (27.22%), Postives = 89/169 (52.66%), Query Frame = 3
Query:  156 AGAPVTAEILRLLPEVRLVVATSAGLNHIDVVECRRRGVALANAGNVFSEDVADYALGLLIDVLRKLSAADCFVRQGLWPINAEFPLGSKLGGKRVGIVGLGSIGSEVAKRLEAFGCCV----SYNSRNKKPSVTYPFYPDVCELAANSDVLIICCALTDQTHHLINKQ 650
            +G  VT +++    +++++     G+++IDV     +G+ + NA +  S  VA+  +GL++   R +  A   +++G W  + +   G +L GK +G++GLG IG +V KR +AFG  +     Y  +    S+      D+ EL   +D + +   LT +T H+I ++
Sbjct:   49 SGTKVTRDVIEKAEKLKVIGRAGVGVDNIDVEAATEKGIIVVNAPDASSISVAELTMGLMLAAARNIPQATASLKRGEW--DRKRFKGIELYGKTLGVIGLGRIGQQVVKRAKAFGMNIIGYDPYIPKEVAESMGVELVDDINELCKRADFITLHVPLTPKTRHIIGRE 215          
BLAST of FC920175 vs. ExPASy Swiss-Prot
Match: PTXD_PSEST (Phosphonate dehydrogenase OS=Pseudomonas stutzeri GN=ptxD PE=1 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 2.432e-15
Identity = 59/192 (30.73%), Postives = 93/192 (48.44%), Query Frame = 3
Query:   87 ESALPLHEFLTLHAHSVKAIFSSAGAPVTAEILRLLPEVRLVVATSAGLNHIDVVECRRRGVALANAGNVFSEDVADYALGLLIDVLRKLSAADCFVRQGLWPINAEFPLGSKLGGKRVGIVGLGSIGSEVAKRLEAFGCCVSYNSRNKKPSVT---YPFYPDVC-ELAANSDVLIICCALTDQTHHLINKQ 650
            +S L   E L       +A+ +     V A+ L+  PE+R+V     G ++ DV  C  RGV L    ++ +   A+ A+GL + + R L AAD FVR G +        G+ L    VGI+G+G+IG  +A RL+ +G  + Y+      + T          C EL A+SD +++   L   T HL+N +
Sbjct:   31 DSTLTREEILR-RCRDAQAMMAFMPDRVDADFLQACPELRVVGCALKGFDNFDVDACTARGVWLTFVPDLLTVPTAELAIGLAVGLGRHLRAADAFVRSGEFQGWQPQFYGTGLDNATVGILGMGAIGLAMADRLQGWGATLQYHEAKALDTQTEQRLGLRQVACSELFASSDFILLALPLNADTQHLVNAE 221          
BLAST of FC920175 vs. ExPASy Swiss-Prot
Match: Y2577_STAA8 (Putative 2-hydroxyacid dehydrogenase SAOUHSC_02577 OS=Staphylococcus aureus (strain NCTC 8325) GN=SAOUHSC_02577 PE=3 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 7.075e-15
Identity = 52/177 (29.38%), Postives = 85/177 (48.02%), Query Frame = 3
Query:  141 AIFSSAGAPVTAEILRLLPEVRLVVATSAGLNHIDVVECRRRGVALANAGNVFSEDVADYALGLLIDVLRKLSAADCFVR----QGLWPINAEFPLGSKLGGKRVGIVGLGSIGSEVAKRLEAFGCCVSYNSRNKKPSVTYPF---YPDVCELAANSDVLIICCALTDQTHHLINKQ 650
            A+ S     V  +++    +++++    AG N+ID+   R + + + N     +   AD  +GL++ V R++   D   R     G  P+   F  G ++ GK +GI+GLG IGS VA+R  AF   V Y   N+K          Y D+  L  N+D + I  A   + HHLI+ +
Sbjct:   47 ALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNATADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPL---FFRGREVSGKTIGIIGLGEIGSAVARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLIDTE 220          
The following BLAST results are available for this feature:
BLAST of FC920175 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 100
Match NameE-valueIdentityDescription
SERA_METTH4.008e-1832.57D-3-phosphoglycerate dehydrogenase OS=Methanobacte... [more]
GHRB_ERWT91.166e-1732.24Glyoxylate/hydroxypyruvate reductase B OS=Erwinia ... [more]
YGDH_SCHPO3.393e-1736.47Putative 2-hydroxyacid dehydrogenase C1773.17c OS=... [more]
SERA_BACSU9.872e-1729.83D-3-phosphoglycerate dehydrogenase OS=Bacillus sub... [more]
TKRA_BACSU1.425e-1528.07Probable 2-ketogluconate reductase OS=Bacillus sub... [more]
SERA_MYCTU1.425e-1531.61D-3-phosphoglycerate dehydrogenase OS=Mycobacteriu... [more]
SERA_MYCBO1.425e-1531.61D-3-phosphoglycerate dehydrogenase OS=Mycobacteriu... [more]
SERA_METJA1.862e-1527.22D-3-phosphoglycerate dehydrogenase OS=Methanocaldo... [more]
PTXD_PSEST2.432e-1530.73Phosphonate dehydrogenase OS=Pseudomonas stutzeri ... [more]
Y2577_STAA87.075e-1529.38Putative 2-hydroxyacid dehydrogenase SAOUHSC_02577... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionC32005A03EF RVDevelop1 Citrus clementina cDNA clone C32005A03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC920175 ID=FC920175; Name=FC920175; organism=Citrus clementina; type=EST; length=687bp
GGCTACTCTTAAGAAAACCATCAGGCTTCGCTATGTTAGGAGAACAGTTC
TTTACCTCAAACAAATTCCAATACTTGAAAGCATACGAATCAGCGCTTCC
TCTACACGAATTCTTGACACTACACGCGCACTCAGTGAAGGCCATCTTCT
CCAGCGCAGGTGCGCCGGTTACAGCCGAAATCCTCCGCCTGCTTCCAGAG
GTGCGGCTGGTGGTTGCCACAAGCGCCGGCCTTAACCATATTGACGTAGT
AGAGTGCCGCCGCCGCGGTGTGGCTCTGGCCAATGCTGGTAACGTTTTCT
CTGAAGACGTGGCTGATTACGCACTGGGCTTGCTAATTGATGTCCTGAGA
AAACTTTCTGCTGCCGATTGCTTTGTCAGACAAGGGCTTTGGCCTATCAA
CGCTGAATTTCCGCTTGGGTCTAAGTTGGGTGGCAAACGAGTTGGGATTG
TTGGGCTGGGGAGCATTGGGTCTGAAGTTGCCAAAAGGCTTGAAGCTTTC
GGCTGCTGCGTTTCATACAACTCTAGGAATAAAAAGCCTTCTGTTACGTA
CCCTTTCTATCCGGATGTCTGTGAACTTGCAGCTAACAGTGATGTCCTCA
TCATTTGCTGTGCATTGACTGATCAAACACATCACTTGATCAATAAGCAG
TCTTGTTGGCCCTGGGGAAAGAAGGAGTTATTATTAA
back to top