FC920175

Overview
NameFC920175
Unique NameFC920175
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length687
Libraries
Library NameType
RVDevelop1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC920175 vs. ExPASy Swiss-Prot
Match: Y1556_HAEIN (Putative 2-hydroxyacid dehydrogenase HI1556 OS=Haemophilus influenzae GN=HI1556 PE=1 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 4.292e-12
Identity = 44/164 (26.83%), Postives = 83/164 (50.61%), Query Frame = 3
Query:  177 EILRLLPEVRLVVATSAGLNHIDVVECRRRGVALANAGNVFSEDVADYALGLLIDVLRKLSAADCFVRQGLWPINAEF-----PLGSKLGGKRVGIVGLGSIGSEVAKRLEAFGCCVSYNSRNKKPSVTYPFYPDVCELAANSDVLIICCALTDQTHHLINKQS 653
            E L+ LP+++L+  T+ G N++D+V     G+A+ N     S  V ++ +GL+  +   L+       +  W  + +F     P+ + + G  +G+ G G +G+EV +   A G  V Y + +K  +V    Y    E+   +D++ + C LT+ T  LIN ++
Sbjct:   57 ETLQQLPKLKLIAITATGTNNVDLVAAEEMGIAVRNVTGYSSTTVPEHVIGLIFSLKHSLAGWLRDQTEAKWAESKQFCYFDYPI-TDVRGSTLGVFGKGCLGTEVGRLANAVGMKVLY-AEHKDATVCREGYTPFDEVLKQADIVTLHCPLTETTKDLINAET 218          
BLAST of FC920175 vs. ExPASy Swiss-Prot
Match: YN14_YEAST (Putative 2-hydroxyacid dehydrogenase YNL274C OS=Saccharomyces cerevisiae GN=YNL274C PE=1 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 5.606e-12
Identity = 56/164 (34.15%), Postives = 81/164 (49.39%), Query Frame = 3
Query:  183 LRLLPEVRLVVATSAGLNHIDVVECRRRGVALANAGNVFSEDVADYALGLLIDVLRKLSAADCFVRQGLWPINAEFPLGSKLG----GKRVGIVGLGSIGSEVAKRLEAFGC-CVSYNSRNKKPSVTYP--FYPDVCELAANSDVLIICCALTDQTHHLINKQS 653
            L L   V  V  T AG + IDV   ++R + +AN  ++ S   AD  + LL+  LR     +  + +G WP  A    GS  G    GK VGI+GLG IG  + +RL+ FG     Y++R++ PS       Y    E    SD++ +   L   THHLIN ++
Sbjct:   77 LALPSSVVAVCHTGAGYDQIDVEPFKKRHIQVANVPDLVSNATADTHVFLLLGALRNFGIGNRRLIEGNWP-EAGPACGSPFGYDPEGKTVGILGLGRIGRCILERLKPFGFENFIYHNRHQLPSEEEHGCEYVGFEEFLKRSDIVSVNVPLNHNTHHLINAET 239          
BLAST of FC920175 vs. ExPASy Swiss-Prot
Match: GHRB_ESCF3 (Glyoxylate/hydroxypyruvate reductase B OS=Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73) GN=ghrB PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 5.606e-12
Identity = 49/163 (30.06%), Postives = 80/163 (49.08%), Query Frame = 3
Query:  180 ILRLLPEVRLVVATSAGLNHIDVVECRRRGVALANAGNVFSEDVADYALGLLIDVLRKLSAADCFVRQGLW--PINAEFPLGSKLGGKRVGIVGLGSIGSEVAKRLE-AFGCCVSYNSRNKKPSVTYPF---YPDVCELAANSDVLIICCALTDQTHHLINKQ 650
            +L  +P++R     S G ++ DV     R V L +   V +E VAD  + L++   R++      V+ G W   I  ++  GS +  K +GIVG+G IG  +A+R    F   + YN+R + P     F   Y D+  L   +D + +   LT++THHL   +
Sbjct:   60 LLEKMPKLRATSTISVGYDNFDVDALNARKVLLMHTPTVLTETVADTLMALILATSRRVVEVAERVKAGEWTASIGPDW-FGSDVHHKTLGIVGMGRIGMALAQRAHFGFNMPILYNARRQHPQAEERFNARYCDLDTLLQEADFVCLILPLTEETHHLFGAE 221          
BLAST of FC920175 vs. ExPASy Swiss-Prot
Match: DDH1_SCHPO (2-hydroxyacid dehydrogenase homolog 1 OS=Schizosaccharomyces pombe GN=SPAC186.07c PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 5.606e-12
Identity = 52/166 (31.33%), Postives = 86/166 (51.81%), Query Frame = 3
Query:  168 VTAEILRLLPE--VRLVVATSAGLNHIDVVECRRRGVALANAGNVFSEDVADYALGLLIDVLRKLSAADCFVRQGLWPINAEFPLGSKLGGKRVGIVGLGSIGSEVAKRLE-AFGCCVSYN--SRNKKPSVTYPFYPDVCELAANSDVLIICCALTDQTHHLINKQ 650
            V A+ L++L +   +L+    AG N++D+      G+ +          VA+Y +GLL+ + RK+  A   VR+  + +N    LG  L GK +G++G G IG  VAK L+  FGC V  +    NK+       + +  E+ A +D L + C LT  T HL++++
Sbjct:   56 VDADTLKVLAKNGTKLIALRCAGFNNVDLKAAADNGITVVRVPAYSPYAVAEYTIGLLLSLNRKIHRAYVRVREDDFNLNG--LLGHDLHGKTIGLLGTGRIGGLVAKCLKLGFGCEVLAHDIKPNKELEKFGIQFVEQQEVLAKADFLCLHCPLTPDTEHLVDEK 219          
BLAST of FC920175 vs. ExPASy Swiss-Prot
Match: GHRB_YERE8 (Glyoxylate/hydroxypyruvate reductase B OS=Yersinia enterocolitica serotype O:8 / biotype 1B (strain 8081) GN=ghrB PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 7.321e-12
Identity = 60/208 (28.85%), Postives = 93/208 (44.71%), Query Frame = 3
Query:   42 EQFFTSNKFQYLKAYESALPLHEFLTLHAHSVKAIFSSAGAPVTAEILRLLPEVRLVVATSAGLNHIDVVECRRRGVALANAGNVFSEDVADYALGLLIDVLRKLSAADCFVRQGLWPIN-AEFPLGSKLGGKRVGIVGLGSIGSEVAKRLE-AFGCCVSYNSRNKKPSVTYPFYPDVCE---LAANSDVLIICCALTDQTHHLINKQ 650
            EQ FT N F+ L +        E L+    +   I S  G  +    L   P++R     S G ++ DV    +RG+AL +   V +E VAD  + L++   R++      V+ G W  +  +   G  +  K +GI+G+G IG  +A+R    F   V Y SR    +    F    C    L A  D L I   +T+QT+H+I  +
Sbjct:   20 EQHFTVNSFEGLSSDNQP----ELLSALQQAEGLIGS--GGKIDQAFLERAPKLRAASTISVGYDNFDVDALSQRGIALMHTPTVLTETVADTMMALVLSSARRVVELAERVKAGEWQDSIGDDWFGVDVHHKTIGILGMGRIGMALAQRAHFGFSMPVLYTSRRPHEAAEKRFGARRCSLDTLLAEVDFLCITLPMTEQTYHMIGPE 221          
BLAST of FC920175 vs. ExPASy Swiss-Prot
Match: GHRB_ECOL6 (Glyoxylate/hydroxypyruvate reductase B OS=Escherichia coli O6 GN=ghrB PE=3 SV=2)

HSP 1 Score: 70.8626 bits (172), Expect = 7.321e-12
Identity = 57/207 (27.54%), Postives = 93/207 (44.93%), Query Frame = 3
Query:   75 LKAYESALPLHEFLTLHAHSVK---AIFSSA------GAPVTAEILRLLPEVRLVVATSAGLNHIDVVECRRRGVALANAGNVFSEDVADYALGLLIDVLRKLSAADCFVRQGLW--PINAEFPLGSKLGGKRVGIVGLGSIGSEVAKRLE-AFGCCVSYNSRNKKPSVTYPFYPDVCE---LAANSDVLIICCALTDQTHHLINKQ 650
            L+  +    +H+   L   +V+   AIF+ A         V A +L  +P++R     S G ++ DV     R + L +   V +E VAD  + L++   R++      V+ G W   I  ++  G+ +  K +GIVG+G IG  +A+R    F   + YN+R         F    C    L   SD + +   LTD+THHL   +
Sbjct:   16 LQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTETVADTLMALVLSTARRVVEVAERVKAGEWTASIGPDW-YGTDVHHKTLGIVGMGRIGMALAQRAHFGFNMPILYNARRHHKEAEERFNARYCNLDTLLQESDFVCLILPLTDETHHLFGAE 221          
BLAST of FC920175 vs. ExPASy Swiss-Prot
Match: VANH_ENTFA (D-specific alpha-keto acid dehydrogenase OS=Enterococcus faecalis GN=vanHB PE=2 SV=2)

HSP 1 Score: 69.707 bits (169), Expect = 7.647e-12
Identity = 49/151 (32.45%), Postives = 73/151 (48.34%), Query Frame = 3
Query:  201 VRLVVATSAGLNHIDVVECRRRGVALANAGNVFSED-VADYALGLLIDVLRKLSAADCFVRQGLWPINAEFPLGSKLGGKRVGIVGLGSIGSEVAKRLEAFGCCVSYNSRNKKPSVTYPFYPDVCELAANSDVLIICCALTDQTHHLINKQ 650
            V+ +   S G NHID     R G+++      +S D VADYAL L++  +R   +    V Q  + ++     G +L    VG++G G IG  V KRL  FGC V     ++K       Y  + EL  NSD++ +   L   T HLI ++
Sbjct:   73 VKYISTRSIGCNHIDTTAAERMGISVGTVA--YSPDSVADYALMLMLMAIRGAKSTIHAVAQQNFRLDCV--RGKELRDMTVGVIGTGHIGQAVVKRLRGFGCRVLAYDNSRKIEAD---YVQLDELLKNSDIVTLHVPLCADTRHLIGQR 216          

HSP 2 Score: 20.7866 bits (42), Expect = 7.647e-12
Identity = 10/27 (37.04%), Postives = 15/27 (55.56%), Query Frame = 2
Query:   65 IPILESIRISASSTRILDTTRALSEGH 145
            IP L S  ISA + ++    + +S GH
Sbjct:   30 IPTLISDAISADNAKLAAGNQCISVGH 56          
BLAST of FC920175 vs. ExPASy Swiss-Prot
Match: SERA_MACFA (D-3-phosphoglycerate dehydrogenase OS=Macaca fascicularis GN=PHGDH PE=2 SV=4)

HSP 1 Score: 70.4774 bits (171), Expect = 9.562e-12
Identity = 38/132 (28.79%), Postives = 73/132 (55.30%), Query Frame = 3
Query:  108 EFLTLHAHSVKAIFSSAGAPVTAEILRLLPEVRLVVATSAGLNHIDVVECRRRGVALANAGNVFSEDVADYALGLLIDVLRKLSAADCFVRQGLWPINAEFPLGSKLGGKRVGIVGLGSIGSEVAKRLEAFG 503
            E L       + +   +   VTA+++    ++++V     G++++D+    R+GV + N  N  S   A+   G+++ + R++  A   ++ G W    +F +G++L GK +GI+GLG IG EVA R+++FG
Sbjct:   39 EELIAELQDCEGLIVRSATKVTADVINAAEKLQVVGRAGTGVDNVDLEAATRKGVLVMNTPNGNSLSAAELTCGMIMCLARQIPQATASMKDGKWE-RKKF-MGTELNGKTLGILGLGRIGREVATRMQSFG 168          
BLAST of FC920175 vs. ExPASy Swiss-Prot
Match: GHRB_SHISS (Glyoxylate/hydroxypyruvate reductase B OS=Shigella sonnei (strain Ss046) GN=ghrB PE=3 SV=2)

HSP 1 Score: 70.4774 bits (171), Expect = 9.562e-12
Identity = 57/207 (27.54%), Postives = 94/207 (45.41%), Query Frame = 3
Query:   75 LKAYESALPLHEFLTLHAHSVK---AIFSSA------GAPVTAEILRLLPEVRLVVATSAGLNHIDVVECRRRGVALANAGNVFSEDVADYALGLLIDVLRKLSAADCFVRQGLW--PINAEFPLGSKLGGKRVGIVGLGSIGSEVAKRLE-AFGCCVSYNSRNKKPSVTYPF---YPDVCELAANSDVLIICCALTDQTHHLINKQ 650
            L+  +    +H+   L   +V+   AIF+ A         V A +L  +P++      S G ++ DV     R + L +   V +E VAD  + L++   R++      V+ G W   I  ++  G+ +  K +GIVG+G IG  +A+R    F   + YN+R         F   Y D+  L   SD + +   LTD+THHL   +
Sbjct:   16 LQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTETVADTLMALVLSTARRVVEVAERVKAGEWTASIGPDW-YGTDVHHKTLGIVGMGRIGMALAQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAE 221          
BLAST of FC920175 vs. ExPASy Swiss-Prot
Match: SERA_PANTR (D-3-phosphoglycerate dehydrogenase OS=Pan troglodytes GN=PHGDH PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.249e-11
Identity = 37/132 (28.03%), Postives = 73/132 (55.30%), Query Frame = 3
Query:  108 EFLTLHAHSVKAIFSSAGAPVTAEILRLLPEVRLVVATSAGLNHIDVVECRRRGVALANAGNVFSEDVADYALGLLIDVLRKLSAADCFVRQGLWPINAEFPLGSKLGGKRVGIVGLGSIGSEVAKRLEAFG 503
            E L       + +   +   VTA+++    ++++V     G++++D+    R+G+ + N  N  S   A+   G+++ + R++  A   ++ G W    +F +G++L GK +GI+GLG IG EVA R+++FG
Sbjct:   39 EELIAELQDCEGLIVRSATKVTADVINAAEKLQVVGRAGTGVDNVDLEAATRKGILVMNTPNGNSLSAAELTCGMIMCLARQIPQATASMKDGKWE-RKKF-MGTELNGKTLGILGLGRIGREVATRMQSFG 168          
The following BLAST results are available for this feature:
BLAST of FC920175 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 100
Match NameE-valueIdentityDescription
Y1556_HAEIN4.292e-1226.83Putative 2-hydroxyacid dehydrogenase HI1556 OS=Hae... [more]
YN14_YEAST5.606e-1234.15Putative 2-hydroxyacid dehydrogenase YNL274C OS=Sa... [more]
GHRB_ESCF35.606e-1230.06Glyoxylate/hydroxypyruvate reductase B OS=Escheric... [more]
DDH1_SCHPO5.606e-1231.332-hydroxyacid dehydrogenase homolog 1 OS=Schizosac... [more]
GHRB_YERE87.321e-1228.85Glyoxylate/hydroxypyruvate reductase B OS=Yersinia... [more]
GHRB_ECOL67.321e-1227.54Glyoxylate/hydroxypyruvate reductase B OS=Escheric... [more]
VANH_ENTFA7.647e-1232.45D-specific alpha-keto acid dehydrogenase OS=Entero... [more]
SERA_MACFA9.562e-1228.79D-3-phosphoglycerate dehydrogenase OS=Macaca fasci... [more]
GHRB_SHISS9.562e-1227.54Glyoxylate/hydroxypyruvate reductase B OS=Shigella... [more]
SERA_PANTR1.249e-1128.03D-3-phosphoglycerate dehydrogenase OS=Pan troglody... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionC32005A03EF RVDevelop1 Citrus clementina cDNA clone C32005A03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC920175 ID=FC920175; Name=FC920175; organism=Citrus clementina; type=EST; length=687bp
GGCTACTCTTAAGAAAACCATCAGGCTTCGCTATGTTAGGAGAACAGTTC
TTTACCTCAAACAAATTCCAATACTTGAAAGCATACGAATCAGCGCTTCC
TCTACACGAATTCTTGACACTACACGCGCACTCAGTGAAGGCCATCTTCT
CCAGCGCAGGTGCGCCGGTTACAGCCGAAATCCTCCGCCTGCTTCCAGAG
GTGCGGCTGGTGGTTGCCACAAGCGCCGGCCTTAACCATATTGACGTAGT
AGAGTGCCGCCGCCGCGGTGTGGCTCTGGCCAATGCTGGTAACGTTTTCT
CTGAAGACGTGGCTGATTACGCACTGGGCTTGCTAATTGATGTCCTGAGA
AAACTTTCTGCTGCCGATTGCTTTGTCAGACAAGGGCTTTGGCCTATCAA
CGCTGAATTTCCGCTTGGGTCTAAGTTGGGTGGCAAACGAGTTGGGATTG
TTGGGCTGGGGAGCATTGGGTCTGAAGTTGCCAAAAGGCTTGAAGCTTTC
GGCTGCTGCGTTTCATACAACTCTAGGAATAAAAAGCCTTCTGTTACGTA
CCCTTTCTATCCGGATGTCTGTGAACTTGCAGCTAACAGTGATGTCCTCA
TCATTTGCTGTGCATTGACTGATCAAACACATCACTTGATCAATAAGCAG
TCTTGTTGGCCCTGGGGAAAGAAGGAGTTATTATTAA
back to top