FC920765

Overview
NameFC920765
Unique NameFC920765
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length694
Libraries
Library NameType
RVDevelop1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC920765 vs. ExPASy Swiss-Prot
Match: IFRH_SOLTU (Isoflavone reductase homolog OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 282.722 bits (722), Expect = 8.415e-80
Identity = 144/192 (75.00%), Postives = 162/192 (84.38%), Query Frame = 1
Query:   73 MASKSKILFIGGTGYIGKFTVEASVKAGHPTFVLVRESTISGPSKSQLLDHFKNLGVNLVIGDVLNHESLVKAIKQVDVVISTVGHTLLADQVKIIAAIKEAGNVKRFLPSEFGNDVDRVEGAVEPTKSTYDVKAKIRRAVEAEGIPYTYVESYCFDGYFLPNLLQPEATAPPRDKVVILGDGNPKAVFNRK 648
            MA KSKILFIGGTGYIGKF VEAS KAGH TFVLVREST+S P+K++L+D FK+ GV  V GD+ +HESLVKAIKQVDVVISTVGH LLADQVK+IAAIKEAGNVKRF PSEFGNDVDRV  AVEP K+ ++ KA+IRR VEAEGIP+TYV ++ F GY LPNL QP A  PP DKVVILG GN KAVFN++
Sbjct:    1 MAGKSKILFIGGTGYIGKFIVEASAKAGHDTFVLVRESTLSNPTKTKLIDTFKSFGVTFVHGDLYDHESLVKAIKQVDVVISTVGHALLADQVKLIAAIKEAGNVKRFFPSEFGNDVDRVH-AVEPAKAAFNTKAQIRRVVEAEGIPFTYVATFFFAGYSLPNLAQPGAAGPPNDKVVILGHGNTKAVFNKE 191          

HSP 2 Score: 36.1946 bits (82), Expect = 8.415e-80
Identity = 15/23 (65.22%), Postives = 20/23 (86.96%), Query Frame = 3
Query:  624 SKSCF*QEDDIGTYTIKAVDDPR 692
            +K+ F +E+DIGTYTI AVDDP+
Sbjct:  184 TKAVFNKEEDIGTYTINAVDDPK 206          
BLAST of FC920765 vs. ExPASy Swiss-Prot
Match: IFRH_ARATH (Isoflavone reductase homolog P3 OS=Arabidopsis thaliana GN=At1g75280 PE=1 SV=1)

HSP 1 Score: 248.825 bits (634), Expect = 1.299e-68
Identity = 130/191 (68.06%), Postives = 150/191 (78.53%), Query Frame = 1
Query:   82 KSKILFIGGTGYIGKFTVEASVKAGHPTFVLVRESTISGPSKSQLLDHFKNLGVNLVIGDVLNHESLVKAIKQVDVVISTVGHTLLADQVKIIAAIKEAGNVKRFLPSEFGNDVDRVEGAVEPTKSTYDVKAKIRRAVEAEGIPYTYVESYCFDGYFLPNLLQ--PEATAPPRDKVVILGDGNPKAVFNRK 648
            KSKIL IGGTGYIGKF VEAS KAGH TF LVRE+T+S P K + +  FK+LGV ++ GD+ +HESLVKAIKQVDVVISTVG   + DQ KII+AIKEAGNVKRFLPSEFG DVDR   AVEP KS +  K +IRR +EAEGIPYTY  + CF GY+LP L+Q  P  T+PPRDKV ILGDGN KAV N++
Sbjct:    5 KSKILVIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHGDLNDHESLVKAIKQVDVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGVDVDRT-SAVEPAKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDKVTILGDGNAKAVINKE 194          

HSP 2 Score: 32.7278 bits (73), Expect = 1.299e-68
Identity = 14/23 (60.87%), Postives = 18/23 (78.26%), Query Frame = 3
Query:  624 SKSCF*QEDDIGTYTIKAVDDPR 692
            +K+   +E+DI  YTIKAVDDPR
Sbjct:  187 AKAVINKEEDIAAYTIKAVDDPR 209          
BLAST of FC920765 vs. ExPASy Swiss-Prot
Match: IFRH_TOBAC (Isoflavone reductase homolog A622 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 245.743 bits (626), Expect = 4.901e-68
Identity = 124/189 (65.61%), Postives = 145/189 (76.72%), Query Frame = 1
Query:   73 MASKSKILFIGGTGYIGKFTVEASVKAGHPTFVLVRESTISGPSKSQLLDHFKNLGVNLVIGDVLNHESLVKAIKQVDVVISTVGHTLLADQVKIIAAIKEAGNVKRFLPSEFGNDVDRVEGAVEPTKSTYDVKAKIRRAVEAEGIPYTYVESYCFDGYFLPNLLQPEATAPPRDKVVILGDGNPKAVF 639
            ++ KSKIL IGGTGYIGK+ VE S K+GHPTF L+REST+  P KS+L+D FK+ GV L+ GD+ N ESL+KAIKQVDVVISTVG     DQV II AIKEAGN+KRFLPSEFG DVD    A+EP  S + +K +IRR +EAEGIPYTYV    F  +FLPNL Q EA  PPRDKVVI GDGNPKA++
Sbjct:    3 VSEKSKILIIGGTGYIGKYLVETSAKSGHPTFALIRESTLKNPEKSKLIDTFKSYGVTLLFGDISNQESLLKAIKQVDVVISTVGGQQFTDQVNIIKAIKEAGNIKRFLPSEFGFDVDHAR-AIEPAASLFALKVRIRRMIEAEGIPYTYVICNWFADFFLPNLGQLEAKTPPRDKVVIFGDGNPKAIY 190          

HSP 2 Score: 33.8834 bits (76), Expect = 4.901e-68
Identity = 14/22 (63.64%), Postives = 19/22 (86.36%), Query Frame = 3
Query:  627 KSCF*QEDDIGTYTIKAVDDPR 692
            K+ + +E+DI TYTI+AVDDPR
Sbjct:  187 KAIYVKEEDIATYTIEAVDDPR 208          
BLAST of FC920765 vs. ExPASy Swiss-Prot
Match: IFR_CICAR (Isoflavone reductase OS=Cicer arietinum GN=IFR PE=1 SV=1)

HSP 1 Score: 212.231 bits (539), Expect = 5.672e-57
Identity = 116/200 (58.00%), Postives = 143/200 (71.50%), Query Frame = 1
Query:   73 MASKSKILFIGGTGYIGKFTVEASVKAGHPTFVLVREST--ISGPS---------KSQLLDHFKNLGVNLVIGDVLNHESLVKAIKQVDVVISTVGHTLLADQVKIIAAIKEAGNVKRFLPSEFGNDVDRVEGAVEPTKSTYDVKAKIRRAVEAEGIPYTYVESYCFDGYFLPNLLQPEATAPPRDKVVILGDGNPKAVF 639
            MAS+++IL +G TG IG+  V AS+KAG+PT+ L+R++   I+ PS         K +LL  FK  GV L+ GD+ +HE+LVKAIKQVD VI T G  L+ DQVKII AIKEAGNVKRF PSEFG DVDR   AV+P +  +D KA IRR VEAEG+PYTY+  + F GYFL NL Q +AT PPRDKV+ILGDGN K  +
Sbjct:    1 MASQNRILVLGPTGAIGRHVVWASIKAGNPTYALIRKTPGDINKPSLVAAANPESKEELLQSFKAAGVILLEGDMNDHEALVKAIKQVDTVICTFGRLLILDQVKIIKAIKEAGNVKRFFPSEFGLDVDR-HDAVDPVRPVFDEKASIRRVVEAEGVPYTYLCCHAFTGYFLRNLAQFDATEPPRDKVIILGDGNVKGAY 199          

HSP 2 Score: 30.4166 bits (67), Expect = 5.672e-57
Identity = 12/22 (54.55%), Postives = 16/22 (72.73%), Query Frame = 3
Query:  627 KSCF*QEDDIGTYTIKAVDDPR 692
            K  +  E D+GTYTI+A +DPR
Sbjct:  196 KGAYVTEADVGTYTIRAANDPR 217          
BLAST of FC920765 vs. ExPASy Swiss-Prot
Match: IFR_MEDSA (Isoflavone reductase OS=Medicago sativa GN=IFR PE=1 SV=1)

HSP 1 Score: 214.157 bits (544), Expect = 1.639e-56
Identity = 113/200 (56.50%), Postives = 147/200 (73.50%), Query Frame = 1
Query:   73 MASKSKILFIGGTGYIGKFTVEASVKAGHPTFVLVRES----------TISGP-SKSQLLDHFKNLGVNLVIGDVLNHESLVKAIKQVDVVISTVGHTLLADQVKIIAAIKEAGNVKRFLPSEFGNDVDRVEGAVEPTKSTYDVKAKIRRAVEAEGIPYTYVESYCFDGYFLPNLLQPEATAPPRDKVVILGDGNPKAVF 639
            MA+++KIL +G TG IG+  V AS+KAG+PT+ LVR++          T + P +K +L+D++++LGV L+ GD+ +HE+LVKAIKQVD+VI   G  L+ DQVKII AIKEAGNVK+F PSEFG DVDR E AVEP +  ++ KA IRR +EAEG+PYTY+  + F GYFL NL Q + T PPRDKVVILGDGN K  +
Sbjct:    1 MATENKILILGPTGAIGRHIVWASIKAGNPTYALVRKTPGNVNKPKLITAANPETKEELIDNYQSLGVILLEGDINDHETLVKAIKQVDIVICAAGRLLIEDQVKIIKAIKEAGNVKKFFPSEFGLDVDRHE-AVEPVRQVFEEKASIRRVIEAEGVPYTYLCCHAFTGYFLRNLAQLDTTDPPRDKVVILGDGNVKGAY 199          

HSP 2 Score: 26.9498 bits (58), Expect = 1.639e-56
Identity = 10/21 (47.62%), Postives = 15/21 (71.43%), Query Frame = 3
Query:  627 KSCF*QEDDIGTYTIKAVDDP 689
            K  +  E D+GT+TI+A +DP
Sbjct:  196 KGAYVTEADVGTFTIRAANDP 216          
BLAST of FC920765 vs. ExPASy Swiss-Prot
Match: IFR_PEA (Isoflavone reductase OS=Pisum sativum GN=IFR PE=2 SV=1)

HSP 1 Score: 204.912 bits (520), Expect = 7.299e-54
Identity = 109/200 (54.50%), Postives = 143/200 (71.50%), Query Frame = 1
Query:   73 MASKSKILFIGGTGYIGKFTVEASVKAGHPTFVLVREST--ISGP---------SKSQLLDHFKNLGVNLVIGDVLNHESLVKAIKQVDVVISTVGHTLLADQVKIIAAIKEAGNVKRFLPSEFGNDVDRVEGAVEPTKSTYDVKAKIRRAVEAEGIPYTYVESYCFDGYFLPNLLQPEATAPPRDKVVILGDGNPKAVF 639
            MA+++KIL +G TG IG+  V AS+KAG+PT+ LVR+++  ++ P         +K +LL +++  GV L+ GD+ +HE+LV AIKQVD VI   G  L+ DQVK+I AIKEAGNVKRF PSEFG DVDR   AVEP +  ++ KA IRR VE+EG+PYTY+  + F GYFL NL Q +AT PPRDKVVILGDGN +  +
Sbjct:    1 MATENKILILGATGAIGRHIVWASIKAGNPTYALVRKTSDNVNKPKLTEAANPETKEELLKNYQASGVILLEGDINDHETLVNAIKQVDTVICAAGRLLIEDQVKVIKAIKEAGNVKRFFPSEFGLDVDR-HDAVEPVRQVFEEKASIRRVVESEGVPYTYLCCHAFTGYFLRNLAQIDATDPPRDKVVILGDGNVRGAY 199          

HSP 2 Score: 27.335 bits (59), Expect = 7.299e-54
Identity = 10/15 (66.67%), Postives = 13/15 (86.67%), Query Frame = 3
Query:  645 EDDIGTYTIKAVDDP 689
            E D+GTYTI+A +DP
Sbjct:  202 EADVGTYTIRAANDP 216          
BLAST of FC920765 vs. ExPASy Swiss-Prot
Match: IFRH_MAIZE (Isoflavone reductase homolog IRL OS=Zea mays GN=IRL PE=2 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 7.938e-53
Identity = 104/188 (55.32%), Postives = 129/188 (68.62%), Query Frame = 1
Query:   76 ASKSKILFIGGTGYIGKFTVEASVKAGHPTFVLVRESTISGPSKSQLLDHFKNLGVNLVIGDVLNHESLVKAIKQVDVVISTVGHTLLADQVKIIAAIKEAGNVKRFLPSEFGNDVDRVEGAVEPTKSTYDVKAKIRRAVEAEGIPYTYVESYCFDGYFLPNLLQPEATAPPRDKVVILGDGNPKAVF 639
            + KSKIL +GGTGY+G+  V AS + GHPT  LVR++  S P+K+ LL  F++ GV L+ GD+ +  SLV A+K  DVVIS +G   +ADQ +++ AIKEAGNVKRF PSEFG DVDR  G VEP KS    K  IRRA EA GIPYTY  +  F G+ LP + Q  A  PP DK V+LGDG+ KAVF
Sbjct:    3 SEKSKILVVGGTGYLGRHVVAASARLGHPTSALVRDTAPSDPAKAALLKSFQDAGVTLLKGDLYDQASLVSAVKGADVVISVLGSMQIADQSRLVDAIKEAGNVKRFFPSEFGLDVDRT-GIVEPAKSILGAKVGIRRATEAAGIPYTYAVAGFFAGFGLPKVGQVLAPGPPADKAVVLGDGDTKAVF 189          

HSP 2 Score: 31.5722 bits (70), Expect = 7.938e-53
Identity = 13/23 (56.52%), Postives = 17/23 (73.91%), Query Frame = 3
Query:  624 SKSCF*QEDDIGTYTIKAVDDPR 692
            +K+ F +E DI TYT+ A DDPR
Sbjct:  185 TKAVFVEEGDIATYTVLAADDPR 207          
BLAST of FC920765 vs. ExPASy Swiss-Prot
Match: IFRH_LUPAL (Isoflavone reductase homolog OS=Lupinus albus PE=2 SV=1)

HSP 1 Score: 174.481 bits (441), Expect = 1.302e-46
Identity = 93/192 (48.44%), Postives = 125/192 (65.10%), Query Frame = 1
Query:   82 KSKILFIGGTGYIGKFTVEASVKAGHPTFVLVRESTISGPSKSQLLDHFKNLGVNLVIGDVLNHESLVKAIKQVDVVISTVG------HTLLADQVKIIAAIKEAGNVKRFLPSEFGNDVDRVEGAVEPTKSTYDVKAKIRRAVEAEGIPYTYVESYCFDGYFLPNLLQPEATAPPRDKVVILGDGNPKAVF 639
            KSK+L +GGTGY+G+  V+AS++ GH TF+L R        K Q+L  FK  G  LV     +H+SLV A+K VDVVI T+       H LL  Q+K++ AIK+AGN+KRFLPSEFG D   +  A+EP + T+D K  +R+A+E   IP+TY+ + CF GYF  NL Q +   PPRDKV++ GDGN K V+
Sbjct:    3 KSKVLVVGGTGYVGRRIVKASLEHGHETFILQRPEIGLDIEKLQILLSFKKQGAILVEASFSDHKSLVDAVKLVDVVICTMSGVHFRSHNLLT-QLKLVEAIKDAGNIKRFLPSEFGMDPALMGHALEPGRVTFDEKMTVRKAIEEANIPFTYISANCFAGYFAGNLSQMKTLLPPRDKVLLYGDGNVKPVY 193          

HSP 2 Score: 33.4982 bits (75), Expect = 1.302e-46
Identity = 13/22 (59.09%), Postives = 16/22 (72.73%), Query Frame = 3
Query:  627 KSCF*QEDDIGTYTIKAVDDPR 692
            K  +  EDD+ TYTIK +DDPR
Sbjct:  190 KPVYMDEDDVATYTIKTIDDPR 211          
BLAST of FC920765 vs. ExPASy Swiss-Prot
Match: IGS1_PETHY (Isoeugenol synthase 1 OS=Petunia hybrida GN=IGS1 PE=1 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 2.816e-43
Identity = 90/188 (47.87%), Postives = 122/188 (64.89%), Query Frame = 1
Query:   82 KSKILFIGGTGYIGKFTVEASVKAGHPTFVLVRE-STISGPSKSQLLDHFKNLGVNLVIGDVLNHESLVKAIKQVDVVISTVGHTLLADQVKIIAAIKEAGNVKRFLPSEFGNDVDRVEGAVEPTKSTYDVKAKIRRAVEAEGIPYTYVESYCFDGYFLPNLLQPEATAPPRDKVVILGDGNPKAVFN 642
            K KIL +G TGY+GK+ V+AS+  GHPT+  V      S  SK QLL  F++LGV +  G++  H+ LV   K+VD+VIST+      +Q+K+I AIKEAGN+KRF+PSEFGN+VDRV  A+   ++  D K KIRRA EA GIP+T+V +     YF+  LL P   +   ++V I G G+ KAV N
Sbjct:    5 KGKILILGATGYLGKYMVKASISLGHPTYAYVMPLKKNSDDSKLQLLKEFESLGVTIFYGELSEHDKLVAVFKEVDIVISTLAVPQYLEQLKVIEAIKEAGNIKRFVPSEFGNEVDRVR-ALPRFQAVLDNKKKIRRATEAAGIPFTFVSANSLTAYFVDYLLHPRQKS---EQVTIYGSGDAKAVLN 188          

HSP 2 Score: 30.0314 bits (66), Expect = 2.816e-43
Identity = 12/23 (52.17%), Postives = 16/23 (69.57%), Query Frame = 3
Query:  624 SKSCF*QEDDIGTYTIKAVDDPR 692
            +K+    E+D+  YTIKA DDPR
Sbjct:  183 AKAVLNYEEDVAAYTIKAADDPR 205          
BLAST of FC920765 vs. ExPASy Swiss-Prot
Match: EGS1_OCIBA (Eugenol synthase 1 OS=Ocimum basilicum GN=EGS1 PE=1 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 1.058e-42
Identity = 90/193 (46.63%), Postives = 124/193 (64.25%), Query Frame = 1
Query:   64 VSEMASKSKILFIGGTGYIGKFTVEASVKAGHPTFVLVRESTISGPSKSQLLDHFKNLGVNLVIGDVLNHESLVKAIKQVDVVISTVGHTLLADQVKIIAAIKEAGNVKRFLPSEFGNDVDRVEGAVEPTKSTYDVKAKIRRAVEAEGIPYTYVESYCFDGYFLPNLLQPEATAPPRDKVVILGDGNPKAVFN 642
            + E   KSKIL  GGTGYIG   V+ S+K GHPT+V  R ++    SK+ LLD F++LG  +V G++  HE LV+ +K+VDVVIS +    + DQ KI+ AIK AGN+KRFLPS+FG + DR+  A+ P ++  + K  IRRA+E   IPYTYV + CF  YF+  LL+P     P+D++ + G G  K   N
Sbjct:    1 MEENGMKSKILIFGGTGYIGNHMVKGSLKLGHPTYVFTRPNS----SKTTLLDEFQSLGAIIVKGELDEHEKLVELMKKVDVVISALAFPQILDQFKILEAIKVAGNIKRFLPSDFGVEEDRI-NALPPFEALIERKRMIRRAIEEANIPYTYVSANCFASYFINYLLRP---YDPKDEITVYGTGEAKFAMN 185          

HSP 2 Score: 26.9498 bits (58), Expect = 1.058e-42
Identity = 11/16 (68.75%), Postives = 11/16 (68.75%), Query Frame = 3
Query:  645 EDDIGTYTIKAVDDPR 692
            E DIG YTIK   DPR
Sbjct:  187 EQDIGLYTIKVATDPR 202          
The following BLAST results are available for this feature:
BLAST of FC920765 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 11
Match NameE-valueIdentityDescription
IFRH_SOLTU8.415e-8075.00Isoflavone reductase homolog OS=Solanum tuberosum ... [more]
IFRH_ARATH1.299e-6868.06Isoflavone reductase homolog P3 OS=Arabidopsis tha... [more]
IFRH_TOBAC4.901e-6865.61Isoflavone reductase homolog A622 OS=Nicotiana tab... [more]
IFR_CICAR5.672e-5758.00Isoflavone reductase OS=Cicer arietinum GN=IFR PE=... [more]
IFR_MEDSA1.639e-5656.50Isoflavone reductase OS=Medicago sativa GN=IFR PE=... [more]
IFR_PEA7.299e-5454.50Isoflavone reductase OS=Pisum sativum GN=IFR PE=2 ... [more]
IFRH_MAIZE7.938e-5355.32Isoflavone reductase homolog IRL OS=Zea mays GN=IR... [more]
IFRH_LUPAL1.302e-4648.44Isoflavone reductase homolog OS=Lupinus albus PE=2... [more]
IGS1_PETHY2.816e-4347.87Isoeugenol synthase 1 OS=Petunia hybrida GN=IGS1 P... [more]
EGS1_OCIBA1.058e-4246.63Eugenol synthase 1 OS=Ocimum basilicum GN=EGS1 PE=... [more]

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Properties
Property NameValue
Genbank descriptionC32012G07EF RVDevelop1 Citrus clementina cDNA clone C32012G07, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC920765 ID=FC920765; Name=FC920765; organism=Citrus clementina; type=EST; length=694bp
AACCATCCATTTGATCATTTCTAACAAAACGAAAAAAAAAATCTCAGTAA
TTGAACTGAACGAGTCTCAGAAATGGCATCGAAAAGTAAGATTCTATTCA
TCGGCGGGACAGGGTATATCGGTAAATTCACTGTCGAAGCGAGCGTAAAA
GCCGGCCACCCGACGTTCGTGCTCGTGAGAGAATCCACAATCTCTGGTCC
TTCCAAATCTCAACTCCTCGATCACTTCAAAAACCTCGGCGTCAATCTCG
TCATAGGAGATGTGTTGAATCACGAGAGTTTGGTGAAGGCGATTAAACAA
GTGGACGTGGTGATATCAACGGTCGGTCACACTTTATTAGCTGATCAGGT
CAAGATTATTGCTGCCATTAAAGAAGCCGGTAACGTTAAGAGATTCTTAC
CATCGGAATTTGGAAATGATGTTGATCGAGTCGAAGGCGCCGTAGAGCCA
ACAAAATCGACGTATGACGTAAAGGCGAAAATCCGCCGAGCCGTTGAGGC
AGAAGGCATTCCGTACACTTACGTAGAATCTTATTGTTTTGATGGCTATT
TCCTCCCCAATTTGTTGCAGCCTGAGGCCACTGCCCCCCCTAGGGATAAG
GTTGTCATCTTAGGCGATGGAAATCCAAAAGCTGTTTTTAACAGGAAGAT
GATATTGGAACCTACACTATCAAAGCGGTTGATGATCCGAGAAC
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