FC921244

Overview
NameFC921244
Unique NameFC921244
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length748
Libraries
Library NameType
RVDevelopNcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of FC921244 vs. ExPASy Swiss-Prot
Match: ISPF_SHESA (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Shewanella sp. (strain ANA-3) GN=ispF PE=3 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 5.336e-30
Identity = 62/125 (49.60%), Postives = 85/125 (68.00%), Query Frame = 2 
Query:  302 LPFRVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGAD 676
            +  R+GHGFD+H+     PLI+ G+ VP+E G  AHSDGDV+LH + DAILGA+ L DIG+ FPD+D  +KGA S V ++    L    G+E+GNLD T+I Q PK++P+ E +R  L+  L AD
Sbjct:    1 MKIRIGHGFDVHKFGEARPLILCGVEVPYETGLVAHSDGDVVLHAISDAILGAMALGDIGKHFPDTDAAYKGADSRVLLRHCYALARAKGFELGNLDVTIIAQAPKMAPHIEDMRQVLAADLNAD 125
[Close]
BLAST of FC921244 vs. ExPASy Swiss-Prot
Match: ISPF_MANSM (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Mannheimia succiniciproducens (strain MBEL55E) GN=ispF PE=3 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 5.336e-30
Identity = 59/117 (50.43%), Postives = 83/117 (70.94%), Query Frame = 2 
Query:  311 RVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSE 661
            R+GHGFD+H      PLIIGG+ VP+  G  AHSDGDV LH + DA+LGAL L DIG++FPD+D ++K   S + ++EA R + E GY+IGN+D T+I Q PK+ P+ + +R  ++E
Sbjct:    3 RIGHGFDVHAFGEARPLIIGGVEVPYHTGFIAHSDGDVALHALTDALLGALALGDIGKLFPDTDMQFKNIDSRILLREAFRRVQEKGYKIGNVDVTIIAQAPKMRPHIDAMRAVIAE 119
[Close]
BLAST of FC921244 vs. ExPASy Swiss-Prot
Match: ISPF_HAEIG (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Haemophilus influenzae (strain PittGG) GN=ispF PE=3 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 5.336e-30
Identity = 61/122 (50.00%), Postives = 84/122 (68.85%), Query Frame = 2 
Query:  311 RVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGAD 676
            R+GHGFD+H      PLIIGG+ VP+  G  AHSDGDV LH + DAILGA  L DIG++FPD+D ++K A S   ++EA R + E GY+IGN+D T+I Q PK+ P+ + +R  ++E +  D
Sbjct:    3 RIGHGFDVHAFGEDRPLIIGGVEVPYHTGFIAHSDGDVALHALTDAILGAAALGDIGKLFPDTDMQYKNADSRGLLREAFRQVQEKGYKIGNVDITIIAQAPKMRPHIDAMRAKIAEDIQCD 124
[Close]
BLAST of FC921244 vs. ExPASy Swiss-Prot
Match: ISPF_DESHY (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Desulfitobacterium hafniense (strain Y51) GN=ispF PE=3 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 5.336e-30
Identity = 59/122 (48.36%), Postives = 86/122 (70.49%), Query Frame = 2 
Query:  311 RVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGAD 676
            RVG G+D+H L  G PLI+ GI++PHE+G   HSD DVL H ++DA+LGAL L D+G+ FPD+D +++G  S   +++ ++L++E GY IGN+D  +  QRPKL+PY   +R NL+  L  D
Sbjct:    3 RVGIGYDVHALVAGRPLILAGIDIPHEKGLLGHSDADVLTHTLMDALLGALALGDLGKHFPDTDERYRGISSMKLLEQVMKLLEERGYAIGNIDCIIAAQRPKLAPYIPQMRENLARALKTD 124
[Close]
BLAST of FC921244 vs. ExPASy Swiss-Prot
Match: ISPF_DESHD (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Desulfitobacterium hafniense (strain DCB-2 / DSM 10664) GN=ispF PE=3 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 5.336e-30
Identity = 59/122 (48.36%), Postives = 86/122 (70.49%), Query Frame = 2 
Query:  311 RVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGAD 676
            RVG G+D+H L  G PLI+ GI++PHE+G   HSD DVL H ++DA+LGAL L D+G+ FPD+D +++G  S   +++ ++L++E GY IGN+D  +  QRPKL+PY   +R NL+  L  D
Sbjct:    3 RVGIGYDVHALVAGRPLILAGIDIPHEKGLLGHSDADVLTHTLMDALLGALALGDLGKHFPDTDERYRGISSMKLLEQVMKLLEERGYAIGNIDCIIAAQRPKLAPYIPQMRENLARALKTD 124
[Close]
BLAST of FC921244 vs. ExPASy Swiss-Prot
Match: ISPF_CALS8 (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) GN=ispF PE=3 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 5.336e-30
Identity = 57/123 (46.34%), Postives = 86/123 (69.92%), Query Frame = 2 
Query:  308 FRVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGAD 676
            F+VG GFD+HR      LI+GG+ +P E+G   HSD DVL+H ++DAILGA+G  DIG++FPD+D K+K   S V +KE  +L+   G  I N+D T++ Q+PK+SPY + ++TN+++ L  +
Sbjct:    2 FKVGFGFDVHRFSEDRKLILGGVEIPFEKGLLGHSDADVLIHAIIDAILGAMGENDIGRLFPDTDMKYKDISSIVLLKEVSKLLKNKGMSIINIDTTIVAQKPKISPYTDEMKTNIAKALNIE 124
[Close]
BLAST of FC921244 vs. ExPASy Swiss-Prot
Match: ISPF_SHISS (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Shigella sonnei (strain Ss046) GN=ispF PE=3 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 6.969e-30
Identity = 60/120 (50.00%), Postives = 84/120 (70.00%), Query Frame = 2 
Query:  311 RVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLG 670
            R+GHGFD+H      P+IIGG+ +P+E+G  AHSDGDV LH + DA+LGA  L DIG++FPD+DP +KGA S   ++EA R +   GY +GN+D T+I Q PK+ P+   +R  ++E LG
Sbjct:    2 RIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHALTDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQAPKMLPHIPQMRVFIAEDLG 121
[Close]
BLAST of FC921244 vs. ExPASy Swiss-Prot
Match: ISPF_SHIFL (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Shigella flexneri GN=ispF PE=3 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 6.969e-30
Identity = 60/120 (50.00%), Postives = 84/120 (70.00%), Query Frame = 2 
Query:  311 RVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLG 670
            R+GHGFD+H      P+IIGG+ +P+E+G  AHSDGDV LH + DA+LGA  L DIG++FPD+DP +KGA S   ++EA R +   GY +GN+D T+I Q PK+ P+   +R  ++E LG
Sbjct:    2 RIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHALTDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQAPKMLPHIPQMRVFIAEDLG 121
[Close]
BLAST of FC921244 vs. ExPASy Swiss-Prot
Match: ISPF_SHIF8 (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Shigella flexneri serotype 5b (strain 8401) GN=ispF PE=3 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 6.969e-30
Identity = 60/120 (50.00%), Postives = 84/120 (70.00%), Query Frame = 2 
Query:  311 RVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLG 670
            R+GHGFD+H      P+IIGG+ +P+E+G  AHSDGDV LH + DA+LGA  L DIG++FPD+DP +KGA S   ++EA R +   GY +GN+D T+I Q PK+ P+   +R  ++E LG
Sbjct:    2 RIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHALTDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQAPKMLPHIPQMRVFIAEDLG 121
[Close]
BLAST of FC921244 vs. ExPASy Swiss-Prot
Match: ISPF_SHIDS (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Shigella dysenteriae serotype 1 (strain Sd197) GN=ispF PE=3 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 6.969e-30
Identity = 60/120 (50.00%), Postives = 84/120 (70.00%), Query Frame = 2 
Query:  311 RVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLG 670
            R+GHGFD+H      P+IIGG+ +P+E+G  AHSDGDV LH + DA+LGA  L DIG++FPD+DP +KGA S   ++EA R +   GY +GN+D T+I Q PK+ P+   +R  ++E LG
Sbjct:    2 RIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHALTDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQAPKMLPHIPQMRVFIAEDLG 121
The following BLAST results are available for this feature:
BLAST of FC921244 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
ISPF_SHESA5.336e-3049.602-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_MANSM5.336e-3050.432-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_HAEIG5.336e-3050.002-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_DESHY5.336e-3048.362-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_DESHD5.336e-3048.362-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_CALS85.336e-3046.342-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_SHISS6.969e-3050.002-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_SHIFL6.969e-3050.002-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_SHIF86.969e-3050.002-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_SHIDS6.969e-3050.002-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]

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Properties
Property NameValue
Genbank descriptionC32102G10EF RVDevelopN Citrus clementina cDNA clone C32102G10, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC921244 ID=FC921244; Name=FC921244; organism=Citrus clementina; type=EST; length=748bp
TTTGGCTGATACATATCGAGCAGACAGCAGAGCACGAACAGCGTCTCTCC
TCTCTTCGTAAAATTAGATGGTGTTGACGATGGCCGCTCAATCATACACC
ACCATTCCTCTCTACAGCAAAATTACGAATAAGCCACTATGTCCTCCTCC
GTTGAGTTTGAAGCCTCGTAGCTTAACCGCCAAACATCTCCGCACAACAC
AGTCAACGTCGCTGCCTCGTATTTCAGTTTCAGCTGCAGCCACTTCTTCC
ATCGAAGTTAAAGAGTCATCGGCTTCGATCCAACCGTCCAAATCAAAATC
ACTGCCCTTTCGTGTGGGCCACGGGTTTGATCTTCATAGATTGGAGCCTG
GCTACCCTCTGATCATCGGTGGAATCAATGTTCCCCACGAGAGAGGCTGC
GAGGCTCACTCTGACGGTGATGTTTTGCTTCACTGTGTTGTGGATGCAAT
TTTGGGAGCATTGGGTCTTCCTGATATTGGTCAGATATTTCCTGATTCTG
ATCCTAAGTGGAAGGGTGCACCATCCTCTGTTTTTATCAAAGAAGCTGTG
AGACTCATGGATGAGGCCGGCTATGAGATTGGCAACTTGGATGCCACGCT
GATTCTTCAAAGACCAAAATTGAGCCCATACAAGGAGACTATCCGAACCA
ACTTGTCTGAACTGCTAGGAGCAGACCCACCGTTGTAATCTGAAGCGAAA
CTCATGAAAAAGTGATAGCCTGGTGAAATCGAGTATGCACTCAACGTG
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