FC929529

Overview
NameFC929529
Unique NameFC929529
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length852
Libraries
Library NameType
PhII-IIIV1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
FC929529_ssr137 microsatellite FC929529_ssr137:137..148. BLAST: Citrus ESTs to Prunus persica proteins V1
Ccv1_Contig9267 contig Ccv1_Contig9267:2..852. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC929529 vs. ExPASy Swiss-Prot
Match: PIP1_ATRCA (Aquaporin PIP-type OS=Atriplex canescens PE=2 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 1.816e-58
Identity = 55/64 (85.94%), Postives = 59/64 (92.19%), Query Frame = -1 
Query:  406 PITGTGINPARSFGAAVIYNKEKAWDDQWIFWVGPFIGAFVAAFYHQYILRAAAIKALGSFRSN 597
            PITGTGINPARSFGAAVIYNK++ WDD WIFWVGPF+GA  AA YHQY+LRAAAIKALGSFRSN
Sbjct:  216 PITGTGINPARSFGAAVIYNKKRVWDDHWIFWVGPFVGALAAAAYHQYVLRAAAIKALGSFRSN 279

HSP 2 Score: 111.309 bits (277), Expect = 1.816e-58
Identity = 52/64 (81.25%), Postives = 57/64 (89.06%), Query Frame = -2 
Query:  639 KAFQKSYYNRYGGGANELADGYNKGTGLGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLP 830
            KAF K  YN++GGGAN +A GYNKGT  GAE+IGTFVLVYTVFSATDPKRSARDSHVP+LAPLP
Sbjct:  139 KAFMKGPYNQFGGGANSVALGYNKGTAFGAELIGTFVLVYTVFSATDPKRSARDSHVPILAPLP 202

HSP 3 Score: 34.6538 bits (78), Expect = 1.816e-58
Identity = 15/15 (100.00%), Postives = 15/15 (100.00%), Query Frame = -3 
Query:  596 PIGFAVFMVHLATIP 640
            PIGFAVFMVHLATIP
Sbjct:  202 PIGFAVFMVHLATIP 216
[Close]
BLAST of FC929529 vs. ExPASy Swiss-Prot
Match: PIP25_ARATH (Probable aquaporin PIP2-5 OS=Arabidopsis thaliana GN=PIP2-5 PE=1 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 3.300e-57
Identity = 52/63 (82.54%), Postives = 57/63 (90.48%), Query Frame = -1 
Query:  409 PITGTGINPARSFGAAVIYNKEKAWDDQWIFWVGPFIGAFVAAFYHQYILRAAAIKALGSFRS 597
            PITGTGINPARS GAA+IYNK+KAWD  WIFWVGPF GA +AAFYHQ++LRA AIKALGSFRS
Sbjct:  220 PITGTGINPARSLGAAIIYNKDKAWDHHWIFWVGPFAGAAIAAFYHQFVLRAGAIKALGSFRS 282

HSP 2 Score: 114.39 bits (285), Expect = 3.300e-57
Identity = 54/64 (84.38%), Postives = 59/64 (92.19%), Query Frame = -2 
Query:  639 KAFQKSYYNRYGGGANELADGYNKGTGLGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLP 830
            KAFQ +Y+ RYGGGAN L+DGY+ GTG+ AEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLP
Sbjct:  143 KAFQSAYFTRYGGGANGLSDGYSIGTGVAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLP 206

HSP 3 Score: 33.113 bits (74), Expect = 3.300e-57
Identity = 14/15 (93.33%), Postives = 15/15 (100.00%), Query Frame = -3 
Query:  596 PIGFAVFMVHLATIP 640
            PIGFAVF+VHLATIP
Sbjct:  206 PIGFAVFIVHLATIP 220
[Close]
BLAST of FC929529 vs. ExPASy Swiss-Prot
Match: PIP22_ARATH (Aquaporin PIP2-2 OS=Arabidopsis thaliana GN=PIP2-2 PE=1 SV=2)

HSP 1 Score: 122.094 bits (305), Expect = 4.164e-56
Identity = 58/64 (90.62%), Postives = 60/64 (93.75%), Query Frame = -2 
Query:  639 KAFQKSYYNRYGGGANELADGYNKGTGLGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLP 830
            KAFQ SYY+RYGGGAN LADGYN GTGL AEIIGTFVLVYTVFSATDPKR+ARDSHVPVLAPLP
Sbjct:  142 KAFQSSYYDRYGGGANSLADGYNTGTGLAAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLP 205

HSP 2 Score: 118.242 bits (295), Expect = 4.164e-56
Identity = 53/65 (81.54%), Postives = 58/65 (89.23%), Query Frame = -1 
Query:  403 PITGTGINPARSFGAAVIYNKEKAWDDQWIFWVGPFIGAFVAAFYHQYILRAAAIKALGSFRSNA 597
            PITGTGINPARSFGAAVIYNK K WDD WIFWVGPFIGA +AAFYHQ++LRA+  K+LGSFRS A
Sbjct:  219 PITGTGINPARSFGAAVIYNKSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAA 283
[Close]
BLAST of FC929529 vs. ExPASy Swiss-Prot
Match: PIP25_ORYSJ (Aquaporin PIP2-5 OS=Oryza sativa subsp. japonica GN=PIP2-5 PE=2 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 4.605e-56
Identity = 56/64 (87.50%), Postives = 60/64 (93.75%), Query Frame = -2 
Query:  639 KAFQKSYYNRYGGGANELADGYNKGTGLGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLP 830
            K FQ S+Y+RYGGGANELA GY+KGTGL AEIIGTFVLVYTVFSATDPKR+ARDSHVPVLAPLP
Sbjct:  143 KGFQSSFYDRYGGGANELAAGYSKGTGLAAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLP 206

HSP 2 Score: 108.227 bits (269), Expect = 4.605e-56
Identity = 49/65 (75.38%), Postives = 55/65 (84.62%), Query Frame = -1 
Query:  403 PITGTGINPARSFGAAVIYNKEKAWDDQWIFWVGPFIGAFVAAFYHQYILRAAAIKALGSFRSNA 597
            P+TGTGINPARS GAAV+YN  KAW DQWIFWVGPFIGA +AA YHQ +LRA+A +  GSFRSNA
Sbjct:  220 PVTGTGINPARSLGAAVVYNNSKAWSDQWIFWVGPFIGAAIAALYHQIVLRASA-RGYGSFRSNA 283

HSP 3 Score: 34.6538 bits (78), Expect = 4.605e-56
Identity = 15/15 (100.00%), Postives = 15/15 (100.00%), Query Frame = -3 
Query:  596 PIGFAVFMVHLATIP 640
            PIGFAVFMVHLATIP
Sbjct:  206 PIGFAVFMVHLATIP 220
[Close]
BLAST of FC929529 vs. ExPASy Swiss-Prot
Match: PIP21_ARATH (Aquaporin PIP2-1 OS=Arabidopsis thaliana GN=PIP2-1 PE=1 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 9.226e-56
Identity = 58/64 (90.62%), Postives = 59/64 (92.19%), Query Frame = -2 
Query:  639 KAFQKSYYNRYGGGANELADGYNKGTGLGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLP 830
            KAFQ SYY RYGGGAN LADGY+ GTGL AEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLP
Sbjct:  144 KAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLP 207

HSP 2 Score: 118.242 bits (295), Expect = 9.226e-56
Identity = 53/65 (81.54%), Postives = 58/65 (89.23%), Query Frame = -1 
Query:  403 PITGTGINPARSFGAAVIYNKEKAWDDQWIFWVGPFIGAFVAAFYHQYILRAAAIKALGSFRSNA 597
            PITGTGINPARSFGAAVIYNK K WDD WIFWVGPFIGA +AAFYHQ++LRA+  K+LGSFRS A
Sbjct:  221 PITGTGINPARSFGAAVIYNKSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAA 285
[Close]
BLAST of FC929529 vs. ExPASy Swiss-Prot
Match: PIP24_ORYSJ (Aquaporin PIP2-4 OS=Oryza sativa subsp. japonica GN=PIP2-4 PE=2 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 1.087e-54
Identity = 55/64 (85.94%), Postives = 58/64 (90.62%), Query Frame = -2 
Query:  639 KAFQKSYYNRYGGGANELADGYNKGTGLGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLP 830
            K FQ S Y+RYGGGANELA GY+ GTGL AEIIGTFVLVYTVFSATDPKR+ARDSHVPVLAPLP
Sbjct:  146 KGFQSSLYDRYGGGANELAAGYSTGTGLAAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLP 209

HSP 2 Score: 107.457 bits (267), Expect = 1.087e-54
Identity = 50/65 (76.92%), Postives = 54/65 (83.08%), Query Frame = -1 
Query:  403 PITGTGINPARSFGAAVIYNKEKAWDDQWIFWVGPFIGAFVAAFYHQYILRAAAIKALGSFRSNA 597
            PITGTGINPARS G AV+YN  KAW DQWIFWVGPFIGA +AA YHQ ILRA+A +  GSFRSNA
Sbjct:  223 PITGTGINPARSLGVAVVYNNNKAWSDQWIFWVGPFIGAAIAALYHQVILRASA-RGYGSFRSNA 286

HSP 3 Score: 34.6538 bits (78), Expect = 1.087e-54
Identity = 15/15 (100.00%), Postives = 15/15 (100.00%), Query Frame = -3 
Query:  596 PIGFAVFMVHLATIP 640
            PIGFAVFMVHLATIP
Sbjct:  209 PIGFAVFMVHLATIP 223
[Close]
BLAST of FC929529 vs. ExPASy Swiss-Prot
Match: PIP25_MAIZE (Aquaporin PIP2-5 OS=Zea mays GN=PIP2-5 PE=1 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 3.119e-54
Identity = 54/64 (84.38%), Postives = 59/64 (92.19%), Query Frame = -2 
Query:  639 KAFQKSYYNRYGGGANELADGYNKGTGLGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLP 830
            K FQ ++Y RYGGGANEL+ GY+KGTGL AEIIGTFVLVYTVFSATDPKR+ARDSHVPVLAPLP
Sbjct:  144 KGFQSAFYVRYGGGANELSAGYSKGTGLAAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLP 207

HSP 2 Score: 105.916 bits (263), Expect = 3.119e-54
Identity = 50/63 (79.37%), Postives = 54/63 (85.71%), Query Frame = -1 
Query:  409 PITGTGINPARSFGAAVIYNKEKAWDDQWIFWVGPFIGAFVAAFYHQYILRAAAIKALGSFRS 597
            PITGTGINPARS GAAVIYN +KAWDD WIFWVGPFIGA +AA YHQY+LRA+A K LGS  S
Sbjct:  221 PITGTGINPARSLGAAVIYNNDKAWDDHWIFWVGPFIGAAIAAAYHQYVLRASAAK-LGSSAS 282

HSP 3 Score: 34.6538 bits (78), Expect = 3.119e-54
Identity = 15/15 (100.00%), Postives = 15/15 (100.00%), Query Frame = -3 
Query:  596 PIGFAVFMVHLATIP 640
            PIGFAVFMVHLATIP
Sbjct:  207 PIGFAVFMVHLATIP 221
[Close]
BLAST of FC929529 vs. ExPASy Swiss-Prot
Match: PIP26_MAIZE (Aquaporin PIP2-6 OS=Zea mays GN=PIP2-6 PE=2 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 5.282e-54
Identity = 52/64 (81.25%), Postives = 57/64 (89.06%), Query Frame = -2 
Query:  639 KAFQKSYYNRYGGGANELADGYNKGTGLGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLP 830
            K FQ  +Y RYGGGANE++ GY+ GTGL AEIIGTFVLVYTVFSATDPKR+ARDSHVPVLAPLP
Sbjct:  148 KGFQSGFYARYGGGANEVSAGYSTGTGLAAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLP 211

HSP 2 Score: 108.612 bits (270), Expect = 5.282e-54
Identity = 50/65 (76.92%), Postives = 55/65 (84.62%), Query Frame = -1 
Query:  403 PITGTGINPARSFGAAVIYNKEKAWDDQWIFWVGPFIGAFVAAFYHQYILRAAAIKALGSFRSNA 597
            PITGTGINPARS GAAV+YN  KAW DQWIFWVGPFIGA +AA YHQ +LRA+A +  GSFRSNA
Sbjct:  225 PITGTGINPARSLGAAVVYNNSKAWSDQWIFWVGPFIGAAIAALYHQIVLRASA-RGYGSFRSNA 288

HSP 3 Score: 34.6538 bits (78), Expect = 5.282e-54
Identity = 15/15 (100.00%), Postives = 15/15 (100.00%), Query Frame = -3 
Query:  596 PIGFAVFMVHLATIP 640
            PIGFAVFMVHLATIP
Sbjct:  211 PIGFAVFMVHLATIP 225
[Close]
BLAST of FC929529 vs. ExPASy Swiss-Prot
Match: PIP28_ARATH (Probable aquaporin PIP2-8 OS=Arabidopsis thaliana GN=PIP2-8 PE=1 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 8.373e-54
Identity = 57/64 (89.06%), Postives = 58/64 (90.62%), Query Frame = -1 
Query:  406 PITGTGINPARSFGAAVIYNKEKAWDDQWIFWVGPFIGAFVAAFYHQYILRAAAIKALGSFRSN 597
            PITGTGINPARSFGAAVIYN EKAWDD WIFWVGPF+GA  AA YHQYILRAAAIKAL SFRSN
Sbjct:  212 PITGTGINPARSFGAAVIYNNEKAWDDHWIFWVGPFVGALAAAAYHQYILRAAAIKALASFRSN 275

HSP 2 Score: 108.997 bits (271), Expect = 8.373e-54
Identity = 53/64 (82.81%), Postives = 56/64 (87.50%), Query Frame = -2 
Query:  639 KAFQKSYYNRYGGGANELADGYNKGTGLGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLP 830
            KAF  + Y R GGGAN +ADGY+ GT LGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLP
Sbjct:  135 KAFMMTPYKRLGGGANTVADGYSTGTALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLP 198
[Close]
BLAST of FC929529 vs. ExPASy Swiss-Prot
Match: PIP23_ARATH (Aquaporin PIP2-3 OS=Arabidopsis thaliana GN=PIP2-3 PE=1 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 1.091e-53
Identity = 52/65 (80.00%), Postives = 58/65 (89.23%), Query Frame = -1 
Query:  403 PITGTGINPARSFGAAVIYNKEKAWDDQWIFWVGPFIGAFVAAFYHQYILRAAAIKALGSFRSNA 597
            PITGTGINPARSFGAAVI+NK K WDD WIFWVGPFIGA +AAFYHQ++LRA+  K+LGSFRS A
Sbjct:  219 PITGTGINPARSFGAAVIFNKSKPWDDHWIFWVGPFIGATIAAFYHQFVLRASGSKSLGSFRSAA 283

HSP 2 Score: 115.546 bits (288), Expect = 1.091e-53
Identity = 56/64 (87.50%), Postives = 58/64 (90.62%), Query Frame = -2 
Query:  639 KAFQKSYYNRYGGGANELADGYNKGTGLGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLP 830
            KAFQ S+Y  YGGGAN LADGYN GTGL AEIIGTFVLVYTVFSATDPKR+ARDSHVPVLAPLP
Sbjct:  142 KAFQSSHYVNYGGGANFLADGYNTGTGLAAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLP 205
The following BLAST results are available for this feature:
BLAST of FC929529 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 51
Match NameE-valueIdentityDescription
PIP1_ATRCA1.816e-5885.94Aquaporin PIP-type OS=Atriplex canescens PE=2 SV=1[more]
PIP25_ARATH3.300e-5782.54Probable aquaporin PIP2-5 OS=Arabidopsis thaliana ... [more]
PIP22_ARATH4.164e-5690.63Aquaporin PIP2-2 OS=Arabidopsis thaliana GN=PIP2-2... [more]
PIP25_ORYSJ4.605e-5687.50Aquaporin PIP2-5 OS=Oryza sativa subsp. japonica G... [more]
PIP21_ARATH9.226e-5690.63Aquaporin PIP2-1 OS=Arabidopsis thaliana GN=PIP2-1... [more]
PIP24_ORYSJ1.087e-5485.94Aquaporin PIP2-4 OS=Oryza sativa subsp. japonica G... [more]
PIP25_MAIZE3.119e-5484.38Aquaporin PIP2-5 OS=Zea mays GN=PIP2-5 PE=1 SV=1[more]
PIP26_MAIZE5.282e-5481.25Aquaporin PIP2-6 OS=Zea mays GN=PIP2-6 PE=2 SV=1[more]
PIP28_ARATH8.373e-5489.06Probable aquaporin PIP2-8 OS=Arabidopsis thaliana ... [more]
PIP23_ARATH1.091e-5380.00Aquaporin PIP2-3 OS=Arabidopsis thaliana GN=PIP2-3... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAH1AB12RM2 PhII-IIIV1 Citrus clementina cDNA clone KN0AAH1AB12, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC929529 ID=FC929529; Name=FC929529; organism=Citrus clementina; type=EST; length=852bp
TTTTTTTTTACAAAAAGGTCATAGTTTTATTCACTAGAGTTGGGGATTAA
TTAACATTAACAACATAAATACACCACTTGAAATAAAGAAAAAGGAAAGG
ATAAGAAAAATATAGAGAAATAGGATATATAAAAAACACCACCACCACTT
ATAAACACACCCAAAGAAAAAAATTAAAATGGGATACCAAAATCCACCCT
TACACATAGATAAAGCATAGGCATAAAATGCATAATGTTCAAATATCTAC
AATTCAATAGCCCTCTAATTCAGCTCAAATTCAATGGCCCTCTAAAGAGC
TTGAAAAGAGATTTCTTAAAGGGCCTTCGTAACAGACTTGCACTCCTGAT
CATGCTGTAAATTTTTCACCTTGGAAGCACCAGACATCATATTTAAAGCT
TAAGCATTGCTCCTGAAGGATCCAAGAGCCTTAATGGCTGCTGCCCTGAG
AATGTATTGGTGGTAGAATGCAGCAACGAAAGCTCCAATGAATGGTCCAA
CCCAGAAGATCCATTGGTCATCCCAGGCTTTCTCCTTGTTGTAGATCACA
GCAGCTCCAAAACTCCTAGCTGGGTTGATGCCGGTTCCGGTGATGGGGGA
TGGTGGCCAAGTGAACCATGAACACAGCAAACCCAATGGGGGAGTGGTGC
CAAGACCGGAACATGGGAGTCTCTTGCGCTCCTCTTAGGATCAGTGGCAG
AGAAAACAGTGTAGACAAGAACGAAAGTACCGATGATCTCAGCACCCAGT
CCGGTGCCCTTGTTGTATCCATCAGCTAGCTCATTGGCCCCACCTCCATA
CCTGTTGTAGTATGATTTTTGGAAAGCCTTCCAAACCCACACCACAGATT
GC
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