FC929646

Overview
NameFC929646
Unique NameFC929646
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length806
Libraries
Library NameType
PhII-IIIV1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
FC929646_ssr137 microsatellite FC929646_ssr137:137..148. BLAST: Citrus ESTs to Prunus persica proteins V1
Ccv1_Contig9267 contig Ccv1_Contig9267:2..806. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC929646 vs. ExPASy Swiss-Prot
Match: PIP13_MAIZE (Aquaporin PIP1-3/PIP1-4 OS=Zea mays GN=PIP1-3 PE=2 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 1.570e-42
Identity = 41/57 (71.93%), Postives = 47/57 (82.46%), Query Frame = -3 
Query:  427 PITGTGINPARSFGAAVIYNKEKAWDDQWIFWVGPFIGAFVAAFYHQYILRAAAIKA 597
            PITGTGINPARS GAA+IYN++ AW D WIFWVGPFIGA +AA YHQ I+RA   K+
Sbjct:  234 PITGTGINPARSLGAAIIYNRDHAWSDHWIFWVGPFIGAALAAIYHQVIIRAIPFKS 290

HSP 2 Score: 86.6557 bits (213), Expect = 1.570e-42
Identity = 40/50 (80.00%), Postives = 45/50 (90.00%), Query Frame = -1 
Query:  639 ANELADGYNKGTGLGAEIIGTFVLVYTVFSATDXKRSARDSHVPVLAPLP 788
            AN +A GY KG GLGAEI+GTF+LVYTVFSATD KR+ARDSHVP+LAPLP
Sbjct:  171 ANVVAPGYTKGDGLGAEIVGTFILVYTVFSATDAKRNARDSHVPILAPLP 220

HSP 3 Score: 33.4982 bits (75), Expect = 1.570e-42
Identity = 14/15 (93.33%), Postives = 15/15 (100.00%), Query Frame = -2 
Query:  596 PIGFAVFMVHLATIP 640
            PIGFAVF+VHLATIP
Sbjct:  220 PIGFAVFLVHLATIP 234
[Close]
BLAST of FC929646 vs. ExPASy Swiss-Prot
Match: PIP27_ORYSJ (Probable aquaporin PIP2-7 OS=Oryza sativa subsp. japonica GN=PIP2-7 PE=2 SV=2)

HSP 1 Score: 102.064 bits (253), Expect = 1.669e-41
Identity = 46/65 (70.77%), Postives = 52/65 (80.00%), Query Frame = -3 
Query:  403 PITGTGINPARSFGAAVIYNKEKAWDDQWIFWVGPFIGAFVAAFYHQYILRAAAIKALGSFRSNA 597
            PITGTGINPARS GAAV+YN+  AW D WIFWVGP IGAF+AA YH+ +LR  A KAL SFRS +
Sbjct:  223 PITGTGINPARSLGAAVLYNQHAAWKDHWIFWVGPVIGAFLAAAYHKLVLRGEAAKALSSFRSTS 287

HSP 2 Score: 76.6406 bits (187), Expect = 1.669e-41
Identity = 35/50 (70.00%), Postives = 42/50 (84.00%), Query Frame = -1 
Query:  639 ANELADGYNKGTGLGAEIIGTFVLVYTVFSATDXKRSARDSHVPVLAPLP 788
            AN ++DGY+    LGAEI+GTF+LVYTVFSATD KR+ARDS +PVL PLP
Sbjct:  160 ANTVSDGYSAAGALGAEIVGTFILVYTVFSATDPKRTARDSFIPVLVPLP 209

HSP 3 Score: 33.113 bits (74), Expect = 1.669e-41
Identity = 14/15 (93.33%), Postives = 15/15 (100.00%), Query Frame = -2 
Query:  596 PIGFAVFMVHLATIP 640
            PIGFAVF+VHLATIP
Sbjct:  209 PIGFAVFVVHLATIP 223
[Close]
BLAST of FC929646 vs. ExPASy Swiss-Prot
Match: PIP15_MAIZE (Aquaporin PIP1-5 OS=Zea mays GN=PIP1-5 PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 2.170e-41
Identity = 39/57 (68.42%), Postives = 46/57 (80.70%), Query Frame = -3 
Query:  427 PITGTGINPARSFGAAVIYNKEKAWDDQWIFWVGPFIGAFVAAFYHQYILRAAAIKA 597
            PITGTGINPARS GAA++YN+  AW+D WIFWVGPFIGA +AA YH  I+RA   K+
Sbjct:  230 PITGTGINPARSLGAAIVYNRSHAWNDHWIFWVGPFIGAALAAIYHVVIIRALPFKS 286

HSP 2 Score: 86.2705 bits (212), Expect = 2.170e-41
Identity = 41/50 (82.00%), Postives = 44/50 (88.00%), Query Frame = -1 
Query:  639 ANELADGYNKGTGLGAEIIGTFVLVYTVFSATDXKRSARDSHVPVLAPLP 788
            AN +  GY KG GLGAEI+GTFVLVYTVFSATD KRSARDSHVP+LAPLP
Sbjct:  167 ANAVNPGYTKGDGLGAEIVGTFVLVYTVFSATDAKRSARDSHVPILAPLP 216

HSP 3 Score: 33.4982 bits (75), Expect = 2.170e-41
Identity = 14/15 (93.33%), Postives = 15/15 (100.00%), Query Frame = -2 
Query:  596 PIGFAVFMVHLATIP 640
            PIGFAVF+VHLATIP
Sbjct:  216 PIGFAVFLVHLATIP 230
[Close]
BLAST of FC929646 vs. ExPASy Swiss-Prot
Match: PIP1_SOLLC (Probable aquaporin PIP-type pTOM75 OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 3.669e-41
Identity = 40/52 (76.92%), Postives = 44/52 (84.62%), Query Frame = -3 
Query:  442 PITGTGINPARSFGAAVIYNKEKAWDDQWIFWVGPFIGAFVAAFYHQYILRA 597
            PITGTGINPARS GAA+IYN E AW+D WIFWVGP IGA +AA YHQ I+RA
Sbjct:  229 PITGTGINPARSLGAAIIYNDEHAWNDHWIFWVGPMIGAALAAIYHQIIIRA 280

HSP 2 Score: 85.5001 bits (210), Expect = 3.669e-41
Identity = 42/56 (75.00%), Postives = 47/56 (83.93%), Query Frame = -1 
Query:  639 QQVWRWANELADGYNKGTGLGAEIIGTFVLVYTVFSATDXKRSARDSHVPVLAPLP 806
            Q++   AN +  GY KG GLGAEIIGTFVLVYTVFSATD KR+ARDSHVP+LAPLP
Sbjct:  160 QRLGGGANVVNPGYTKGDGLGAEIIGTFVLVYTVFSATDAKRNARDSHVPILAPLP 215

HSP 3 Score: 33.4982 bits (75), Expect = 3.669e-41
Identity = 14/15 (93.33%), Postives = 15/15 (100.00%), Query Frame = -2 
Query:  596 PIGFAVFMVHLATIP 640
            PIGFAVF+VHLATIP
Sbjct:  215 PIGFAVFLVHLATIP 229
[Close]
BLAST of FC929646 vs. ExPASy Swiss-Prot
Match: PIP13_ORYSJ (Aquaporin PIP 1-3 OS=Oryza sativa subsp. japonica GN=PIP1-3 PE=2 SV=2)

HSP 1 Score: 90.1225 bits (222), Expect = 1.363e-40
Identity = 38/57 (66.67%), Postives = 45/57 (78.95%), Query Frame = -3 
Query:  427 PITGTGINPARSFGAAVIYNKEKAWDDQWIFWVGPFIGAFVAAFYHQYILRAAAIKA 597
            PITGTGINPARS GAA++YN+  AW D WIFWVGPFIGA +AA YH  ++RA   K+
Sbjct:  230 PITGTGINPARSLGAAIVYNRAHAWHDHWIFWVGPFIGAALAAIYHVVVIRAIPFKS 286

HSP 2 Score: 85.1149 bits (209), Expect = 1.363e-40
Identity = 40/50 (80.00%), Postives = 44/50 (88.00%), Query Frame = -1 
Query:  639 ANELADGYNKGTGLGAEIIGTFVLVYTVFSATDXKRSARDSHVPVLAPLP 788
            AN +  GY KG GLGAEI+GTFVLVYTVFSATD KR+ARDSHVP+LAPLP
Sbjct:  167 ANAVNPGYTKGDGLGAEIVGTFVLVYTVFSATDAKRNARDSHVPILAPLP 216

HSP 3 Score: 33.4982 bits (75), Expect = 1.363e-40
Identity = 14/15 (93.33%), Postives = 15/15 (100.00%), Query Frame = -2 
Query:  596 PIGFAVFMVHLATIP 640
            PIGFAVF+VHLATIP
Sbjct:  216 PIGFAVFLVHLATIP 230
[Close]
BLAST of FC929646 vs. ExPASy Swiss-Prot
Match: PIP11_MAIZE (Aquaporin PIP1-1 OS=Zea mays GN=PIP1-1 PE=1 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 1.881e-39
Identity = 41/56 (73.21%), Postives = 45/56 (80.36%), Query Frame = -3 
Query:  427 ITGTGINPARSFGAAVIYNKEKAWDDQWIFWVGPFIGAFVAAFYHQYILRAAAIKA 594
            ITGTGINPARS GAAVIYN+  AW D WIFWVGPFIGA +AA YHQ I+RA   K+
Sbjct:  230 ITGTGINPARSLGAAVIYNQHHAWADHWIFWVGPFIGAALAAIYHQVIIRAIPFKS 285

HSP 2 Score: 84.3445 bits (207), Expect = 1.881e-39
Identity = 39/49 (79.59%), Postives = 43/49 (87.76%), Query Frame = -1 
Query:  639 NELADGYNKGTGLGAEIIGTFVLVYTVFSATDXKRSARDSHVPVLAPLP 785
            N +A GY KG GLGAEI+GTF+LVYTVFSATD KR ARDSHVP+LAPLP
Sbjct:  167 NVVAPGYTKGDGLGAEIVGTFILVYTVFSATDAKRRARDSHVPILAPLP 215

HSP 3 Score: 29.6462 bits (65), Expect = 1.881e-39
Identity = 12/14 (85.71%), Postives = 14/14 (100.00%), Query Frame = -2 
Query:  599 PIGFAVFMVHLATI 640
            PIGFAVF+VHLAT+
Sbjct:  215 PIGFAVFLVHLATM 228
[Close]
BLAST of FC929646 vs. ExPASy Swiss-Prot
Match: PIP16_MAIZE (Aquaporin PIP1-6 OS=Zea mays GN=PIP1-6 PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 6.976e-39
Identity = 39/50 (78.00%), Postives = 44/50 (88.00%), Query Frame = -1 
Query:  639 ANELADGYNKGTGLGAEIIGTFVLVYTVFSATDXKRSARDSHVPVLAPLP 788
            AN ++ GY KG GLGAE++GTFVLVYTVFSATD KR+ARDSHVP LAPLP
Sbjct:  173 ANAVSPGYTKGDGLGAEVVGTFVLVYTVFSATDAKRTARDSHVPALAPLP 222

HSP 2 Score: 84.7297 bits (208), Expect = 6.976e-39
Identity = 37/57 (64.91%), Postives = 42/57 (73.68%), Query Frame = -3 
Query:  427 PITGTGINPARSFGAAVIYNKEKAWDDQWIFWVGPFIGAFVAAFYHQYILRAAAIKA 597
            PITGTGINPARS GAA+IY+    W   WIFWVGPF GA +AA YHQ +LRA   K+
Sbjct:  236 PITGTGINPARSLGAAIIYDNPHGWHGHWIFWVGPFAGAALAAVYHQVVLRAIPFKS 292

HSP 3 Score: 33.4982 bits (75), Expect = 6.976e-39
Identity = 14/15 (93.33%), Postives = 15/15 (100.00%), Query Frame = -2 
Query:  596 PIGFAVFMVHLATIP 640
            PIGFAVF+VHLATIP
Sbjct:  222 PIGFAVFLVHLATIP 236
[Close]
BLAST of FC929646 vs. ExPASy Swiss-Prot
Match: PIP12_ORYSJ (Probable aquaporin PIP1-2 OS=Oryza sativa subsp. japonica GN=PIP1-2 PE=2 SV=3)

HSP 1 Score: 95.5153 bits (236), Expect = 8.427e-39
Identity = 41/57 (71.93%), Postives = 47/57 (82.46%), Query Frame = -3 
Query:  427 PITGTGINPARSFGAAVIYNKEKAWDDQWIFWVGPFIGAFVAAFYHQYILRAAAIKA 597
            PITGTGINPARS GAA+IYN+  AWDD WIFWVGPFIGA +AA YHQ ++RA   K+
Sbjct:  230 PITGTGINPARSLGAAIIYNRGHAWDDHWIFWVGPFIGAALAAIYHQVVIRAIPFKS 286

HSP 2 Score: 86.6557 bits (213), Expect = 8.427e-39
Identity = 40/50 (80.00%), Postives = 45/50 (90.00%), Query Frame = -1 
Query:  639 ANELADGYNKGTGLGAEIIGTFVLVYTVFSATDXKRSARDSHVPVLAPLP 788
            AN +A GY KG GLGAEI+GTF+LVYTVFSATD KR+ARDSHVP+LAPLP
Sbjct:  167 ANVVAPGYTKGDGLGAEIVGTFILVYTVFSATDAKRNARDSHVPILAPLP 216
[Close]
BLAST of FC929646 vs. ExPASy Swiss-Prot
Match: PIP2_PEA (Probable aquaporin PIP-type 7a OS=Pisum sativum GN=TRG-31 PE=2 SV=2)

HSP 1 Score: 91.6633 bits (226), Expect = 6.992e-38
Identity = 38/57 (66.67%), Postives = 47/57 (82.46%), Query Frame = -3 
Query:  427 PITGTGINPARSFGAAVIYNKEKAWDDQWIFWVGPFIGAFVAAFYHQYILRAAAIKA 597
            PITGTGINPARS GAA+++NK+  W+D WIFWVGPFIGA +AA YHQ ++RA   K+
Sbjct:  232 PITGTGINPARSLGAAIVFNKKIGWNDHWIFWVGPFIGAALAALYHQVVIRAIPFKS 288

HSP 2 Score: 87.4261 bits (215), Expect = 6.992e-38
Identity = 41/50 (82.00%), Postives = 45/50 (90.00%), Query Frame = -1 
Query:  639 ANELADGYNKGTGLGAEIIGTFVLVYTVFSATDXKRSARDSHVPVLAPLP 788
            AN +A GY KG GLGAEI+GTF+LVYTVFSATD KRSARDSHVP+LAPLP
Sbjct:  169 ANFVAPGYTKGDGLGAEIVGTFILVYTVFSATDAKRSARDSHVPILAPLP 218
[Close]
BLAST of FC929646 vs. ExPASy Swiss-Prot
Match: PIP28_ORYSJ (Probable aquaporin PIP2-8 OS=Oryza sativa subsp. japonica GN=PIP2-8 PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.077e-35
Identity = 40/50 (80.00%), Postives = 44/50 (88.00%), Query Frame = -1 
Query:  639 ANELADGYNKGTGLGAEIIGTFVLVYTVFSATDXKRSARDSHVPVLAPLP 788
            ANELA GY+ G G+ AE++GTFVLVYTVFSATD KR ARDSHVPVLAPLP
Sbjct:  148 ANELAAGYSAGAGVVAEMVGTFVLVYTVFSATDPKRKARDSHVPVLAPLP 197

HSP 2 Score: 80.4925 bits (197), Expect = 1.077e-35
Identity = 39/62 (62.90%), Postives = 41/62 (66.13%), Query Frame = -3 
Query:  418 PITGTGINPARSFGAAVIYN--KEKAWDDQWIFWVGPFIGAFVAAFYHQYILRAAAIKALGS 597
            PITGTGINPARS G A++      KAW   WIFWVGPF GA  A  YH YILR AA KA  S
Sbjct:  211 PITGTGINPARSLGPALVLGLGTTKAWSHLWIFWVGPFAGAAAAMIYHHYILRGAAAKAFAS 272

HSP 3 Score: 28.4906 bits (62), Expect = 1.077e-35
Identity = 12/15 (80.00%), Postives = 13/15 (86.67%), Query Frame = -2 
Query:  596 PIGFAVFMVHLATIP 640
            PIG AV +VHLATIP
Sbjct:  197 PIGLAVLVVHLATIP 211
The following BLAST results are available for this feature:
BLAST of FC929646 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 49
Match NameE-valueIdentityDescription
PIP13_MAIZE1.570e-4271.93Aquaporin PIP1-3/PIP1-4 OS=Zea mays GN=PIP1-3 PE=2... [more]
PIP27_ORYSJ1.669e-4170.77Probable aquaporin PIP2-7 OS=Oryza sativa subsp. j... [more]
PIP15_MAIZE2.170e-4168.42Aquaporin PIP1-5 OS=Zea mays GN=PIP1-5 PE=2 SV=1[more]
PIP1_SOLLC3.669e-4176.92Probable aquaporin PIP-type pTOM75 OS=Solanum lyco... [more]
PIP13_ORYSJ1.363e-4066.67Aquaporin PIP 1-3 OS=Oryza sativa subsp. japonica ... [more]
PIP11_MAIZE1.881e-3973.21Aquaporin PIP1-1 OS=Zea mays GN=PIP1-1 PE=1 SV=1[more]
PIP16_MAIZE6.976e-3978.00Aquaporin PIP1-6 OS=Zea mays GN=PIP1-6 PE=2 SV=1[more]
PIP12_ORYSJ8.427e-3971.93Probable aquaporin PIP1-2 OS=Oryza sativa subsp. j... [more]
PIP2_PEA6.992e-3866.67Probable aquaporin PIP-type 7a OS=Pisum sativum GN... [more]
PIP28_ORYSJ1.077e-3580.00Probable aquaporin PIP2-8 OS=Oryza sativa subsp. j... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAH1AH11RM2 PhII-IIIV1 Citrus clementina cDNA clone KN0AAH1AH11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC929646 ID=FC929646; Name=FC929646; organism=Citrus clementina; type=EST; length=806bp
TTTTTTTTTACAAAAAGGTCATAGTTTTATTCACTAGAGTTGGGGATTAA
TTAACATTAACAACATAAATACACCACTTGAAATAAAGAAAAAGGAAAGG
ATAAGAAAAATATAGAGAAATAGGATATATAAAAAACACCACCACCACTT
ATAAACACACCCAAAGAAAAAAATTAAAATGGGATACCAAAATCCACCCT
TACACATAGATAAAGCATAGGCATAAAATGCATAATGTTCAAATATCTAC
AATTCAATAGCCCTCTAATTCAGCTCAAATTCAATGGCCCTCTAAAGAGC
TTGAAAAGAGATTTCTTAAAGGGCCTTCGTAACAGACTTGCACTCCTGAT
CATGCTGTAAATTTTTCACCTTGGAAGCACCAGACATCATATTTAAAGCT
TAAGCATTGCTCCTGAAGGATCCAAGAGCCTTAATGGCTGCTGCCCTGAG
AATGTATTGGTGGTAGAATGCAGCAACGAAAGCTCCAATGAATGGTCCAA
CCCAGAAGATCCATTGGTCATCCCAGGCTTTCTCCTTGTTGTAGATCACA
GCAGCTCCAAAACTCCTAGCTGGGTTGATGCCGGTTCCGGTGATGGGGGA
TGGTGGCCAAGTGAACCATGAACACAGCAAACCCAATGGGGGAGTGGTGC
CAAGACCGGAACATGGGAGTCTCTTGCGCTCCTCTTANGATCAGTGGCAG
AGAAAACAGTGTAGACAAGAACGAAAGTACCGATGATCTCAGCACCCAGT
CCGGTGCCCTTGTTGTATCCATCAGCTAGCTCATTGGCCCACCTCCATAC
CTGTTG
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