FC930017

Overview
NameFC930017
Unique NameFC930017
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length688
Libraries
Library NameType
PostHarvC1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig6281 contig Ccv1_Contig6281:166..857. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC930017 vs. ExPASy Swiss-Prot
Match: SUCD_RICBR (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Rickettsia bellii (strain RML369-C) GN=sucD PE=3 SV=1)

HSP 1 Score: 238.81 bits (608), Expect = 5.767e-69
Identity = 122/173 (70.52%), Postives = 137/173 (79.19%), Query Frame = 1 
Query:  115 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGGTEHLGLPVFNSVAEAKAETKANASVIYVPPPF-XXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRVGIVSRSGTLTYEAVFQTTVVGLGQS 630
            A+ V+K T+VICQG TG  GTFH+EQAI YGTKMVGGVTP KGG  HL LP++N+V EAKA+T ANASVIYVPPPF            +++VVCITEGIP  DMVRVK AL   S+TRLIGPNCPGII P ECKIGIMPG+IH+ G +GIVSRSGTLTYEAV QTT VGLGQS
Sbjct:    2 AILVNKKTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKGGKTHLDLPIYNTVHEAKAKTGANASVIYVPPPFAADSILEAIDAGIEIVVCITEGIPVLDMVRVKRAL-VGSRTRLIGPNCPGIITPDECKIGIMPGHIHRKGSIGIVSRSGTLTYEAVAQTTAVGLGQS 173

HSP 2 Score: 43.8986 bits (102), Expect = 5.767e-69
Identity = 17/19 (89.47%), Postives = 18/19 (94.74%), Query Frame = 3 
Query:  630 TCVGIGGDPFNGTNFVDCL 686
            TCVGIGGDP NGTNFVDC+
Sbjct:  174 TCVGIGGDPVNGTNFVDCI 192
[Close]
BLAST of FC930017 vs. ExPASy Swiss-Prot
Match: SUCD_RICFE (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Rickettsia felis GN=sucD PE=3 SV=1)

HSP 1 Score: 235.728 bits (600), Expect = 4.824e-68
Identity = 121/173 (69.94%), Postives = 136/173 (78.61%), Query Frame = 1 
Query:  115 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGGTEHLGLPVFNSVAEAKAETKANASVIYVPPPF-XXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRVGIVSRSGTLTYEAVFQTTVVGLGQS 630
            A+ ++K T+VICQG TG  GTFH+EQAI YGT MVGGVTP KGG  HL LPV+N+V EAKA+T ANASVIYVPP F            +++VVCITEGIP  DMV+VK AL   SKTRLIGPNCPG+I PGECKIGIMPG+IHK G +GIVSRSGTLTYEAV QTT VGLGQS
Sbjct:    2 AILINKKTKVICQGFTGSQGTFHSEQAIAYGTNMVGGVTPGKGGHTHLDLPVYNTVHEAKAKTGANASVIYVPPGFAADSILEAIDAEIEVVVCITEGIPVLDMVKVKRAL-VGSKTRLIGPNCPGVITPGECKIGIMPGHIHKRGTIGIVSRSGTLTYEAVAQTTAVGLGQS 173

HSP 2 Score: 43.8986 bits (102), Expect = 4.824e-68
Identity = 17/19 (89.47%), Postives = 18/19 (94.74%), Query Frame = 3 
Query:  630 TCVGIGGDPFNGTNFVDCL 686
            TCVGIGGDP NGTNFVDC+
Sbjct:  174 TCVGIGGDPVNGTNFVDCI 192
[Close]
BLAST of FC930017 vs. ExPASy Swiss-Prot
Match: SUCD_RICCN (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Rickettsia conorii GN=sucD PE=3 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 1.395e-67
Identity = 121/173 (69.94%), Postives = 136/173 (78.61%), Query Frame = 1 
Query:  115 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGGTEHLGLPVFNSVAEAKAETKANASVIYVPPPF-XXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRVGIVSRSGTLTYEAVFQTTVVGLGQS 630
            A+ ++K T+VICQG TG  GTFH+EQAI YGT MVGGVTP KGG  HL LPV+N+V EAKA+T ANASVIYVPP F            +++VVCITEGIP  DMV+VK AL   SKTRLIGPNCPG+I PGECKIGIMPG+IHK G +GIVSRSGTLTYEAV QTT VGLGQS
Sbjct:    2 AILINKKTQVICQGFTGLQGTFHSEQAIAYGTNMVGGVTPGKGGHTHLDLPVYNTVHEAKAKTGANASVIYVPPGFAADSILEAIDAEIEVVVCITEGIPVLDMVKVKRAL-VGSKTRLIGPNCPGVITPGECKIGIMPGHIHKRGTIGIVSRSGTLTYEAVAQTTAVGLGQS 173

HSP 2 Score: 43.5134 bits (101), Expect = 1.395e-67
Identity = 16/19 (84.21%), Postives = 18/19 (94.74%), Query Frame = 3 
Query:  630 TCVGIGGDPFNGTNFVDCL 686
            TC+GIGGDP NGTNFVDC+
Sbjct:  174 TCIGIGGDPVNGTNFVDCI 192
[Close]
BLAST of FC930017 vs. ExPASy Swiss-Prot
Match: SUCD_RICTY (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Rickettsia typhi GN=sucD PE=3 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 5.260e-67
Identity = 121/173 (69.94%), Postives = 136/173 (78.61%), Query Frame = 1 
Query:  115 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGGTEHLGLPVFNSVAEAKAETKANASVIYVPPPF-XXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRVGIVSRSGTLTYEAVFQTTVVGLGQS 630
            A+ ++K T+VICQG TG  GTFH+EQAI YGT MVGGVTP KGG  HL LPV+N+V EAKA+T ANASVIYVPP F            +++VVCITEGIP  DMV+VK AL   SKTRLIGPNCPG+I PGECKIGIMPG+IHK G +GIVSRSGTLTYEAV QTT VGLGQS
Sbjct:    2 AILINKKTKVICQGFTGSQGTFHSEQAIAYGTNMVGGVTPGKGGHTHLNLPVYNTVHEAKAKTDANASVIYVPPGFAADSILEAIDAKIEVVVCITEGIPVLDMVKVKRAL-IGSKTRLIGPNCPGVITPGECKIGIMPGHIHKTGIIGIVSRSGTLTYEAVAQTTAVGLGQS 173

HSP 2 Score: 40.0466 bits (92), Expect = 5.260e-67
Identity = 14/19 (73.68%), Postives = 18/19 (94.74%), Query Frame = 3 
Query:  630 TCVGIGGDPFNGTNFVDCL 686
            TC+GIGGDP NGT+FV+C+
Sbjct:  174 TCIGIGGDPVNGTSFVECI 192
[Close]
BLAST of FC930017 vs. ExPASy Swiss-Prot
Match: SUCD_RICPR (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Rickettsia prowazekii GN=sucD PE=3 SV=1)

HSP 1 Score: 233.417 bits (594), Expect = 8.945e-67
Identity = 119/173 (68.79%), Postives = 135/173 (78.03%), Query Frame = 1 
Query:  115 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGGTEHLGLPVFNSVAEAKAETKANASVIYVPPPF-XXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRVGIVSRSGTLTYEAVFQTTVVGLGQS 630
            A+ ++K T+VICQG TG  GTFH+EQAI YGT MVGGVTP KGG  HL LPV+N+V EAKA+T ANASVIYVPP F            +++VVCITEGIP  DM++VK AL   SKTRLIGPNCPG+I PGECKIGIMPG+IHK G +GIVSRSGTLTYEAV QTT  GLGQS
Sbjct:    2 AILINKKTKVICQGFTGSQGTFHSEQAIAYGTNMVGGVTPGKGGHTHLNLPVYNTVHEAKAKTGANASVIYVPPGFAADSILEAIDAKIEVVVCITEGIPVLDMIKVKRAL-IGSKTRLIGPNCPGVITPGECKIGIMPGHIHKIGDIGIVSRSGTLTYEAVAQTTAAGLGQS 173

HSP 2 Score: 41.9726 bits (97), Expect = 8.945e-67
Identity = 16/19 (84.21%), Postives = 18/19 (94.74%), Query Frame = 3 
Query:  630 TCVGIGGDPFNGTNFVDCL 686
            TCVGIGGDP NGT+FVDC+
Sbjct:  174 TCVGIGGDPVNGTSFVDCI 192
[Close]
BLAST of FC930017 vs. ExPASy Swiss-Prot
Match: SUCD_COXBU (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Coxiella burnetii GN=sucD PE=3 SV=2)

HSP 1 Score: 232.261 bits (591), Expect = 1.386e-64
Identity = 114/173 (65.90%), Postives = 134/173 (77.46%), Query Frame = 1 
Query:  115 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGGTEHLGLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXX-LDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRVGIVSRSGTLTYEAVFQTTVVGLGQS 630
            ++ +DK TRV+CQG TGK GTFH+EQAIEYGT+MVGGVTP KGG  HLGLP+FNSV EA  ET A+A++I+VP PF            + LVVCITEGIP  DM+ VKA L +    R+IGPNCPG+I PGECKIGIMPG+IH+ G+VGIVSRSGTLTYEAV QTT +G GQS
Sbjct:    2 SILIDKETRVLCQGFTGKQGTFHSEQAIEYGTQMVGGVTPGKGGQSHLGLPIFNSVHEAVEETSADATMIFVPAPFCKDSIIEAVDAGVRLVVCITEGIPVLDMLEVKAYLRQHPDVRMIGPNCPGVITPGECKIGIMPGHIHQRGKVGIVSRSGTLTYEAVNQTTQMGFGQS 174

HSP 2 Score: 35.8094 bits (81), Expect = 1.386e-64
Identity = 14/19 (73.68%), Postives = 16/19 (84.21%), Query Frame = 3 
Query:  630 TCVGIGGDPFNGTNFVDCL 686
            TCVGIGGDP  GT+F+D L
Sbjct:  175 TCVGIGGDPIPGTSFIDVL 193
[Close]
BLAST of FC930017 vs. ExPASy Swiss-Prot
Match: SUCA_CAEEL (Probable succinyl-CoA ligase [GDP-forming] subunit alpha, mitochondrial OS=Caenorhabditis elegans GN=C05G5.4 PE=2 SV=1)

HSP 1 Score: 219.935 bits (559), Expect = 1.158e-63
Identity = 110/170 (64.71%), Postives = 131/170 (77.06%), Query Frame = 1 
Query:  124 VDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGGTEHLGLPVFNSVAEAKAETKANASVIYVPPPFXXXXXXXXXXX-LDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRVGIVSRSGTLTYEAVFQTTVVGLGQS 630
            ++K+T+VI QG TGK GTFH +Q +EY TK+VGGV   K GTEHLGLPVF +V+EA+ +T A+ASVIYVP               + LVVCITEGIPQHDMVRVK+ L +Q+KTRL+GPNCPGII   +CKIGIMPG+IHK G +GIVSRSGTLTYEAV QTT VG GQ+
Sbjct:   30 INKDTKVIVQGFTGKQGTFHGKQMLEYNTKVVGGVNANKAGTEHLGLPVFKNVSEARNKTGADASVIYVPASAAGSAIEEAMDAEIPLVVCITEGIPQHDMVRVKSRLLKQNKTRLVGPNCPGIISADQCKIGIMPGHIHKRGCIGIVSRSGTLTYEAVHQTTQVGFGQT 199

HSP 2 Score: 45.0542 bits (105), Expect = 1.158e-63
Identity = 17/18 (94.44%), Postives = 18/18 (100.00%), Query Frame = 3 
Query:  633 CVGIGGDPFNGTNFVDCL 686
            CVGIGGDPFNGTNF+DCL
Sbjct:  201 CVGIGGDPFNGTNFIDCL 218
[Close]
BLAST of FC930017 vs. ExPASy Swiss-Prot
Match: SUCD_ECOLI (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Escherichia coli (strain K12) GN=sucD PE=1 SV=2)

HSP 1 Score: 228.794 bits (582), Expect = 1.159e-63
Identity = 115/173 (66.47%), Postives = 132/173 (76.30%), Query Frame = 1 
Query:  115 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGGTEHLGLPVFNSVAEAKAETKANASVIYVPPPF-XXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRVGIVSRSGTLTYEAVFQTTVVGLGQS 630
            ++ +DKNT+VICQG TG  GTFH+EQAI YGTKMVGGVTP KGGT HLGLPVFN+V EA A T A ASVIYVP PF            + L++ ITEGIP  DM+ VK  L+ ++  R+IGPNCPG+I PGECKIGI PG+IHKPG+VGIVSRSGTLTYEAV QTT  G GQS
Sbjct:    2 SILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKGGTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPTLDMLTVKVKLD-EAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYEAVKQTTDYGFGQS 173

HSP 2 Score: 36.1946 bits (82), Expect = 1.159e-63
Identity = 14/19 (73.68%), Postives = 16/19 (84.21%), Query Frame = 3 
Query:  630 TCVGIGGDPFNGTNFVDCL 686
            TCVGIGGDP  G+NF+D L
Sbjct:  174 TCVGIGGDPIPGSNFIDIL 192
[Close]
BLAST of FC930017 vs. ExPASy Swiss-Prot
Match: SUCD_ECOL6 (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Escherichia coli O6 GN=sucD PE=3 SV=2)

HSP 1 Score: 228.794 bits (582), Expect = 1.159e-63
Identity = 115/173 (66.47%), Postives = 132/173 (76.30%), Query Frame = 1 
Query:  115 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGGTEHLGLPVFNSVAEAKAETKANASVIYVPPPF-XXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRVGIVSRSGTLTYEAVFQTTVVGLGQS 630
            ++ +DKNT+VICQG TG  GTFH+EQAI YGTKMVGGVTP KGGT HLGLPVFN+V EA A T A ASVIYVP PF            + L++ ITEGIP  DM+ VK  L+ ++  R+IGPNCPG+I PGECKIGI PG+IHKPG+VGIVSRSGTLTYEAV QTT  G GQS
Sbjct:    2 SILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKGGTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPTLDMLTVKVKLD-EAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYEAVKQTTDYGFGQS 173

HSP 2 Score: 36.1946 bits (82), Expect = 1.159e-63
Identity = 14/19 (73.68%), Postives = 16/19 (84.21%), Query Frame = 3 
Query:  630 TCVGIGGDPFNGTNFVDCL 686
            TCVGIGGDP  G+NF+D L
Sbjct:  174 TCVGIGGDPIPGSNFIDIL 192
[Close]
BLAST of FC930017 vs. ExPASy Swiss-Prot
Match: SUCD_ECO57 (Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Escherichia coli O157:H7 GN=sucD PE=3 SV=2)

HSP 1 Score: 228.794 bits (582), Expect = 1.159e-63
Identity = 115/173 (66.47%), Postives = 132/173 (76.30%), Query Frame = 1 
Query:  115 AVFVDKNTRVICQGITGKNGTFHTEQAIEYGTKMVGGVTPKKGGTEHLGLPVFNSVAEAKAETKANASVIYVPPPF-XXXXXXXXXXXLDLVVCITEGIPQHDMVRVKAALNRQSKTRLIGPNCPGIIKPGECKIGIMPGYIHKPGRVGIVSRSGTLTYEAVFQTTVVGLGQS 630
            ++ +DKNT+VICQG TG  GTFH+EQAI YGTKMVGGVTP KGGT HLGLPVFN+V EA A T A ASVIYVP PF            + L++ ITEGIP  DM+ VK  L+ ++  R+IGPNCPG+I PGECKIGI PG+IHKPG+VGIVSRSGTLTYEAV QTT  G GQS
Sbjct:    2 SILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKGGTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPTLDMLTVKVKLD-EAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYEAVKQTTDYGFGQS 173

HSP 2 Score: 36.1946 bits (82), Expect = 1.159e-63
Identity = 14/19 (73.68%), Postives = 16/19 (84.21%), Query Frame = 3 
Query:  630 TCVGIGGDPFNGTNFVDCL 686
            TCVGIGGDP  G+NF+D L
Sbjct:  174 TCVGIGGDPIPGSNFIDIL 192
The following BLAST results are available for this feature:
BLAST of FC930017 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 46
Match NameE-valueIdentityDescription
SUCD_RICBR5.767e-6970.52Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD_RICFE4.824e-6869.94Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD_RICCN1.395e-6769.94Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD_RICTY5.260e-6769.94Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD_RICPR8.945e-6768.79Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD_COXBU1.386e-6465.90Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCA_CAEEL1.158e-6364.71Probable succinyl-CoA ligase [GDP-forming] subunit... [more]
SUCD_ECOLI1.159e-6366.47Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD_ECOL61.159e-6366.47Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]
SUCD_ECO571.159e-6366.47Succinyl-CoA ligase [ADP-forming] subunit alpha OS... [more]

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Properties
Property NameValue
Genbank descriptionC34002H06EF PostHarvC1 Citrus clementina cDNA clone C34002H06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC930017 ID=FC930017; Name=FC930017; organism=Citrus clementina; type=EST; length=688bp
GGATGTCGCAGACCCTCTACTGCTTCTTCCGTTTCACATTACAGATTATC
AACGTCGGCGGGGCCGGCGGCCCACTCTCGATATTATGGGTCGTCGGCGG
CGGCGTCTCAGCCGGCAGTTTTCGTCGACAAAAACACTCGAGTTATATGC
CAAGGCATCACCGGAAAAAACGGCACGTTCCATACCGAACAAGCCATCGA
ATACGGCACCAAAATGGTGGGTGGCGTGACGCCGAAGAAGGGTGGGACTG
AGCACTTGGGACTTCCTGTATTCAACTCGGTTGCTGAAGCTAAGGCTGAA
ACGAAGGCTAATGCTTCTGTCATTTATGTTCCTCCGCCATTTGCTGCTGC
TATCATGGAGGCTATGGAGGCTGAGTTGGATCTTGTCGTCTGTATCACCG
AAGGAATCCCTCAACACGATATGGTGCGAGTGAAAGCTGCTCTAAACAGG
CAATCTAAAACTCGGCTGATTGGTCCCAACTGCCCTGGTATCATCAAACC
TGGAGAATGTAAGATTGGCATTATGCCTGGGTATATTCACAAACCTGGGC
GGGTTGGAATTGTTTCTCGATCTGGTACCTTGACCTACGAAGCAGTTTTT
CAAACAACTGTAGTTGGCCTTGGACAATCACCTGTGTAGGTATAGGTGGG
GATCCTTTTAATGGGACAAACTTTGTTGATTGCCTTGA
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