FC930545

Overview
NameFC930545
Unique NameFC930545
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length720
Libraries
Library NameType
PostHarvC1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
FC930545_ssr23 microsatellite FC930545_ssr23:23..44. BLAST: Citrus ESTs to Prunus persica proteins V1
Ccv1_Contig15173 contig Ccv1_Contig15173:1..721. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC930545 vs. ExPASy Swiss-Prot
Match: 1433_MESCR (14-3-3-like protein OS=Mesembryanthemum crystallinum GN=GBF PE=2 SV=1)

HSP 1 Score: 267.314 bits (682), Expect = 1.690e-71
Identity = 134/199 (67.34%), Postives = 165/199 (82.91%), Query Frame = 1 
Query:   91 EKEREHHVYLARLAEQAERYDEMVEAMKKVAKLD--VELTVEERNLVSVGYKNVIGARRASWRILSSIEQKEEAKGNEQNVKRIKEYRQRVEDELAKICNDILSVIDEHLIPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQ 681
            E  RE +VY+A+LAEQAERY+EMVE M+KVAK+    EL+VEERNL+SV YKNVIGARRASWRI+SSIEQKEE++GNE +V  IKEYR ++E EL+KIC+ IL++++ HLIPS+++ ES VFY KMKGDY+RYLAEFK   +RKEAA+++L           +EL PTHPIRLGLALNFSVFYYEILNSP+RAC+LAKQ
Sbjct:    4 ESSREENVYMAKLAEQAERYEEMVEFMEKVAKMTDTEELSVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNEDHVSTIKEYRGKIETELSKICDGILNLLESHLIPSASTAESKVFYLKMKGDYHRYLAEFKTGAERKEAAENTLLAYKSAQDIALAELAPTHPIRLGLALNFSVFYYEILNSPDRACNLAKQ 202

HSP 2 Score: 23.8682 bits (50), Expect = 1.690e-71
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 3 
Query:  681 AFDEAIAELDSL 716
            AFDEAI+ELD+L
Sbjct:  203 AFDEAISELDTL 214
[Close]
BLAST of FC930545 vs. ExPASy Swiss-Prot
Match: 1433_LILLO (14-3-3-like protein OS=Lilium longiflorum PE=2 SV=1)

HSP 1 Score: 266.929 bits (681), Expect = 2.205e-71
Identity = 135/203 (66.50%), Postives = 164/203 (80.79%), Query Frame = 1 
Query:   79 LNEMEKEREHHVYLARLAEQAERYDEMVEAMKKVAKL--DVELTVEERNLVSVGYKNVIGARRASWRILSSIEQKEEAKGNEQNVKRIKEYRQRVEDELAKICNDILSVIDEHLIPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQ 681
            ++  E  RE +VY+A+LAEQAERY+EMVE M+KVA+     ELTVEERNL+SV YKNVIGARRASWRI+SSIEQKEE++GNE +V  IK+YR ++E EL+KIC+ IL ++D HL+PSST+ ES VFY KMKGDY+RYLAEFK   +RKEAA+ +L           +EL PTHPIRLGLALNFSVFYYEILNSP+RAC+LAKQ
Sbjct:    1 MSPAEPSREENVYMAKLAEQAERYEEMVEFMEKVARTVDTEELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNEDHVALIKDYRGKIEAELSKICDGILKLLDSHLVPSSTAPESKVFYLKMKGDYHRYLAEFKSGAERKEAAESTLLAYKSAQDIALAELAPTHPIRLGLALNFSVFYYEILNSPDRACNLAKQ 203

HSP 2 Score: 23.8682 bits (50), Expect = 2.205e-71
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 3 
Query:  681 AFDEAIAELDSL 716
            AFDEAI+ELD+L
Sbjct:  204 AFDEAISELDTL 215
[Close]
BLAST of FC930545 vs. ExPASy Swiss-Prot
Match: 14333_ORYSJ (14-3-3-like protein GF14-C OS=Oryza sativa subsp. japonica GN=GF14C PE=1 SV=1)

HSP 1 Score: 265.774 bits (678), Expect = 4.891e-71
Identity = 135/196 (68.88%), Postives = 164/196 (83.67%), Query Frame = 1 
Query:  100 REHHVYLARLAEQAERYDEMVEAMKKVAK-LDVE-LTVEERNLVSVGYKNVIGARRASWRILSSIEQKEEAKGNEQNVKRIKEYRQRVEDELAKICNDILSVIDEHLIPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQ 681
            RE +VY+A+LAEQAERY+EMVE M+KVAK +DVE LTVEERNL+SV YKNVIGARRASWRI+SSIEQKEE +GNE++V  IKEYR ++E EL+KIC+ IL ++D HL+PSST+ ES VFY KMKGDY+RYLAEFK   +RKEAA+ ++           ++L PTHPIRLGLALNFSVFYYEILNSP++AC+LAKQ
Sbjct:    3 REENVYMAKLAEQAERYEEMVEYMEKVAKTVDVEELTVEERNLLSVAYKNVIGARRASWRIVSSIEQKEEGRGNEEHVTLIKEYRGKIEAELSKICDGILKLLDSHLVPSSTAAESKVFYLKMKGDYHRYLAEFKTGAERKEAAESTMVAYKAAQDIALADLAPTHPIRLGLALNFSVFYYEILNSPDKACNLAKQ 198

HSP 2 Score: 23.8682 bits (50), Expect = 4.891e-71
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 3 
Query:  681 AFDEAIAELDSL 716
            AFDEAI+ELD+L
Sbjct:  199 AFDEAISELDTL 210
[Close]
BLAST of FC930545 vs. ExPASy Swiss-Prot
Match: BMH2_YEAST (Protein BMH2 OS=Saccharomyces cerevisiae GN=BMH2 PE=1 SV=3)

HSP 1 Score: 264.618 bits (675), Expect = 8.313e-71
Identity = 135/199 (67.84%), Postives = 159/199 (79.90%), Query Frame = 1 
Query:   88 MEKEREHHVYLARLAEQAERYDEMVEAMKKVAKLDVELTVEERNLVSVGYKNVIGARRASWRILSSIEQKEEAKG-NEQNVKRIKEYRQRVEDELAKICNDILSVIDEHLIPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQ 681
            M + RE  VYLA+LAEQAERY+EMVE MK VA    EL+VEERNL+SV YKNVIGARRASWRI+SSIEQKEE+K  +E  V+ I+ YR ++E EL KI +DILSV+D HLIPS+T+GES VFYYKMKGDY+RYLAEF   D R++A + SL+          +ELPPTHPIRLGLALNFSVFYYEI NSP++ACHLAKQ
Sbjct:    1 MSQTREDSVYLAKLAEQAERYEEMVENMKAVASSGQELSVEERNLLSVAYKNVIGARRASWRIVSSIEQKEESKEKSEHQVELIRSYRSKIETELTKISDDILSVLDSHLIPSATTGESKVFYYKMKGDYHRYLAEFSSGDAREKATNSSLEAYKTASEIATTELPPTHPIRLGLALNFSVFYYEIQNSPDKACHLAKQ 199

HSP 2 Score: 24.2534 bits (51), Expect = 8.313e-71
Identity = 10/13 (76.92%), Postives = 13/13 (100.00%), Query Frame = 3 
Query:  681 AFDEAIAELDSLN 719
            AFD+AIAELD+L+
Sbjct:  200 AFDDAIAELDTLS 212
[Close]
BLAST of FC930545 vs. ExPASy Swiss-Prot
Match: 14332_MAIZE (14-3-3-like protein GF14-12 OS=Zea mays GN=GRF2 PE=2 SV=2)

HSP 1 Score: 264.618 bits (675), Expect = 8.316e-71
Identity = 134/199 (67.34%), Postives = 159/199 (79.90%), Query Frame = 1 
Query:   91 EKEREHHVYLARLAEQAERYDEMVEAMKKVAKL--DVELTVEERNLVSVGYKNVIGARRASWRILSSIEQKEEAKGNEQNVKRIKEYRQRVEDELAKICNDILSVIDEHLIPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQ 681
            E  RE +VY+A+LAEQAERY+EMVE M+KVAK     ELTVEERNL+SV YKNVIGARRASWRI+SSIEQKEE +GNE  V  IK+YR ++E EL KIC+ IL +++ HL+PSST+ ES VFY KMKGDYYRYLAEFK   +RK+AA++++           +EL PTHPIRLGLALNFSVFYYEILNSP+RAC LAKQ
Sbjct:    5 ELSREENVYMAKLAEQAERYEEMVEFMEKVAKTVDSEELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEEGRGNEDRVTLIKDYRGKIETELTKICDGILKLLESHLVPSSTAPESKVFYLKMKGDYYRYLAEFKTGAERKDAAENTMVAYKAAQDIALAELAPTHPIRLGLALNFSVFYYEILNSPDRACSLAKQ 203

HSP 2 Score: 24.2534 bits (51), Expect = 8.316e-71
Identity = 10/13 (76.92%), Postives = 13/13 (100.00%), Query Frame = 3 
Query:  681 AFDEAIAELDSLN 719
            AFDEAI+ELD+L+
Sbjct:  204 AFDEAISELDTLS 216
[Close]
BLAST of FC930545 vs. ExPASy Swiss-Prot
Match: 14331_MAIZE (14-3-3-like protein GF14-6 OS=Zea mays GN=GRF1 PE=1 SV=1)

HSP 1 Score: 264.233 bits (674), Expect = 1.085e-70
Identity = 134/199 (67.34%), Postives = 159/199 (79.90%), Query Frame = 1 
Query:   91 EKEREHHVYLARLAEQAERYDEMVEAMKKVAKL--DVELTVEERNLVSVGYKNVIGARRASWRILSSIEQKEEAKGNEQNVKRIKEYRQRVEDELAKICNDILSVIDEHLIPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQ 681
            E  RE +VY+A+LAEQAERY+EMVE M+KVAK     ELTVEERNL+SV YKNVIGARRASWRI+SSIEQKEE +GNE  V  IK+YR ++E EL KIC+ IL +++ HL+PSST+ ES VFY KMKGDYYRYLAEFK   +RK+AA++++           +EL PTHPIRLGLALNFSVFYYEILNSP+RAC LAKQ
Sbjct:    5 ELSREENVYMAKLAEQAERYEEMVEFMEKVAKTVDSEELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEEGRGNEDRVTLIKDYRGKIETELTKICDGILKLLETHLVPSSTAPESKVFYLKMKGDYYRYLAEFKTGAERKDAAENTMVAYKAAQDIALAELAPTHPIRLGLALNFSVFYYEILNSPDRACSLAKQ 203

HSP 2 Score: 24.2534 bits (51), Expect = 1.085e-70
Identity = 10/13 (76.92%), Postives = 13/13 (100.00%), Query Frame = 3 
Query:  681 AFDEAIAELDSLN 719
            AFDEAI+ELD+L+
Sbjct:  204 AFDEAISELDTLS 216
[Close]
BLAST of FC930545 vs. ExPASy Swiss-Prot
Match: 14333_ARATH (14-3-3-like protein GF14 psi OS=Arabidopsis thaliana GN=GRF3 PE=1 SV=2)

HSP 1 Score: 264.233 bits (674), Expect = 1.415e-70
Identity = 136/196 (69.39%), Postives = 163/196 (83.16%), Query Frame = 1 
Query:  100 REHHVYLARLAEQAERYDEMVEAMKKVAK-LDVE-LTVEERNLVSVGYKNVIGARRASWRILSSIEQKEEAKGNEQNVKRIKEYRQRVEDELAKICNDILSVIDEHLIPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQ 681
            RE +VY+A+LAEQAERY+EMVE M+KVAK +DVE L+VEERNL+SV YKNVIGARRASWRI+SSIEQKEE+KGNE +V  IK+YR ++E EL+KIC+ IL+V++ HLIPS++  ES VFY KMKGDY+RYLAEFK   +RKEAA+ +L           +EL PTHPIRLGLALNFSVFYYEILNSP+RAC LAKQ
Sbjct:    4 REENVYMAKLAEQAERYEEMVEFMEKVAKTVDVEELSVEERNLLSVAYKNVIGARRASWRIISSIEQKEESKGNEDHVAIIKDYRGKIESELSKICDGILNVLEAHLIPSASPAESKVFYLKMKGDYHRYLAEFKAGAERKEAAESTLVAYKSASDIATAELAPTHPIRLGLALNFSVFYYEILNSPDRACSLAKQ 199

HSP 2 Score: 23.8682 bits (50), Expect = 1.415e-70
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 3 
Query:  681 AFDEAIAELDSL 716
            AFD+AIAELD+L
Sbjct:  200 AFDDAIAELDTL 211
[Close]
BLAST of FC930545 vs. ExPASy Swiss-Prot
Match: 14334_SOLLC (14-3-3 protein 4 OS=Solanum lycopersicum GN=TFT4 PE=2 SV=1)

HSP 1 Score: 263.848 bits (673), Expect = 1.845e-70
Identity = 134/199 (67.34%), Postives = 166/199 (83.42%), Query Frame = 1 
Query:   91 EKEREHHVYLARLAEQAERYDEMVEAMKKVAKL-DVE-LTVEERNLVSVGYKNVIGARRASWRILSSIEQKEEAKGNEQNVKRIKEYRQRVEDELAKICNDILSVIDEHLIPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQ 681
            +  RE +VYLA+LAEQAERY+EM+E M+KVAK  DVE LTVEERNL+SV YKNVIGARRASWRI+SSIEQKEE++GNE +V  IKEYR ++E +L+KIC+ ILS+++ +LIPS+++ ES VF+ KMKGDY+RYLAEFK   +RKEAA+++L           +EL PTHPIRLGLALNFSVFYYEILNSP+RAC+LAKQ
Sbjct:    3 DSSREENVYLAKLAEQAERYEEMIEFMEKVAKTADVEELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNEDHVNTIKEYRSKIEADLSKICDGILSLLESNLIPSASTAESKVFHLKMKGDYHRYLAEFKTGTERKEAAENTLLAYKSAQDIALAELAPTHPIRLGLALNFSVFYYEILNSPDRACNLAKQ 201

HSP 2 Score: 23.8682 bits (50), Expect = 1.845e-70
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 3 
Query:  681 AFDEAIAELDSL 716
            AFDEAI+ELD+L
Sbjct:  202 AFDEAISELDTL 213
[Close]
BLAST of FC930545 vs. ExPASy Swiss-Prot
Match: 1433C_TOBAC (14-3-3-like protein C OS=Nicotiana tabacum PE=1 SV=1)

HSP 1 Score: 262.692 bits (670), Expect = 1.845e-70
Identity = 132/196 (67.35%), Postives = 161/196 (82.14%), Query Frame = 1 
Query:  100 REHHVYLARLAEQAERYDEMVEAMKKVAKL--DVELTVEERNLVSVGYKNVIGARRASWRILSSIEQKEEAKGNEQNVKRIKEYRQRVEDELAKICNDILSVIDEHLIPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQ 681
            RE +VY+A+LAEQAERY+EMVE M+KV+      ELTVEERNL+SV YKNVIGARRASWRI+SSIEQKEE++GNE++V  I+EYR ++E+EL+KIC+ IL ++D  LIPS+ SG+S VFY KMKGDY+RYLAEFK   +RKEAA+ +L           +EL PTHPIRLGLALNFSVFYYEILNSP+RAC+LAKQ
Sbjct:    8 REENVYMAKLAEQAERYEEMVEFMEKVSNSLGSEELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNEEHVNSIREYRSKIENELSKICDGILKLLDAKLIPSAASGDSKVFYLKMKGDYHRYLAEFKTGAERKEAAESTLTAYKAAQDIATTELAPTHPIRLGLALNFSVFYYEILNSPDRACNLAKQ 203

HSP 2 Score: 25.0238 bits (53), Expect = 1.845e-70
Identity = 11/12 (91.67%), Postives = 12/12 (100.00%), Query Frame = 3 
Query:  681 AFDEAIAELDSL 716
            AFDEAIAELD+L
Sbjct:  204 AFDEAIAELDTL 215
[Close]
BLAST of FC930545 vs. ExPASy Swiss-Prot
Match: 1433E_TOBAC (14-3-3-like protein E OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 263.077 bits (671), Expect = 3.136e-70
Identity = 135/199 (67.84%), Postives = 165/199 (82.91%), Query Frame = 1 
Query:   91 EKEREHHVYLARLAEQAERYDEMVEAMKKVAK-LDVE-LTVEERNLVSVGYKNVIGARRASWRILSSIEQKEEAKGNEQNVKRIKEYRQRVEDELAKICNDILSVIDEHLIPSSTSGESTVFYYKMKGDYYRYLAEFKGADDRKEAADHSLKXXXXXXXXXXSELPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQ 681
            E  RE +VY+A+LAEQAERY+EMVE M+KVAK +DVE LTVEERNL+SV YKNVIGARRASWRI+SSIEQKEE++GNE +V  IKEYR ++E EL+KIC+ IL++++ HLIP +++ ES VFY KMKGDY+RYLAEFK   +RKEAA+++L           +EL PTHPIRLGLALNFSVFYYEILNS +RAC+LAKQ
Sbjct:    3 ESTREENVYMAKLAEQAERYEEMVEFMEKVAKTVDVEELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNEDHVSSIKEYRGKIEAELSKICDGILNLLESHLIPVASTAESKVFYLKMKGDYHRYLAEFKTGAERKEAAENTLLAYKSAQDIALAELAPTHPIRLGLALNFSVFYYEILNSSDRACNLAKQ 201

HSP 2 Score: 23.8682 bits (50), Expect = 3.136e-70
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 3 
Query:  681 AFDEAIAELDSL 716
            AFD+AIAELD+L
Sbjct:  202 AFDDAIAELDTL 213
The following BLAST results are available for this feature:
BLAST of FC930545 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 142
Match NameE-valueIdentityDescription
1433_MESCR1.690e-7167.3414-3-3-like protein OS=Mesembryanthemum crystallin... [more]
1433_LILLO2.205e-7166.5014-3-3-like protein OS=Lilium longiflorum PE=2 SV=... [more]
14333_ORYSJ4.891e-7168.8814-3-3-like protein GF14-C OS=Oryza sativa subsp. ... [more]
BMH2_YEAST8.313e-7167.84Protein BMH2 OS=Saccharomyces cerevisiae GN=BMH2 P... [more]
14332_MAIZE8.316e-7167.3414-3-3-like protein GF14-12 OS=Zea mays GN=GRF2 PE... [more]
14331_MAIZE1.085e-7067.3414-3-3-like protein GF14-6 OS=Zea mays GN=GRF1 PE=... [more]
14333_ARATH1.415e-7069.3914-3-3-like protein GF14 psi OS=Arabidopsis thalia... [more]
14334_SOLLC1.845e-7067.3414-3-3 protein 4 OS=Solanum lycopersicum GN=TFT4 P... [more]
1433C_TOBAC1.845e-7067.3514-3-3-like protein C OS=Nicotiana tabacum PE=1 SV... [more]
1433E_TOBAC3.136e-7067.8414-3-3-like protein E OS=Nicotiana tabacum PE=2 SV... [more]

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Properties
Property NameValue
Genbank descriptionC34008F11EF PostHarvC1 Citrus clementina cDNA clone C34008F11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC930545 ID=FC930545; Name=FC930545; organism=Citrus clementina; type=EST; length=720bp
GAAACAAAAAGCAACAAAGCTCAGAGAGAGAGAGAGAGAGAGAGAAGGAA
GAGAGCTAGCGAGAGAAAGAGTGAGTGACTGAACGAAATGGAGAAGGAGA
GAGAGCATCATGTTTACTTGGCCAGACTTGCTGAGCAAGCCGAGAGATAC
GACGAGATGGTTGAAGCCATGAAGAAAGTTGCCAAGTTGGATGTGGAACT
GACTGTGGAGGAGAGGAATCTGGTGTCTGTTGGGTACAAGAATGTGATTG
GGGCAAGAAGAGCATCATGGAGGATTTTGTCTTCCATTGAACAGAAGGAG
GAGGCCAAGGGTAATGAACAAAATGTGAAGAGGATTAAGGAATATAGGCA
GAGGGTTGAAGATGAGCTTGCAAAGATCTGTAATGATATATTGTCTGTCA
TTGATGAACACCTAATCCCATCCTCCACATCTGGAGAATCAACCGTGTTT
TACTATAAGATGAAAGGAGATTATTACCGTTACCTAGCTGAGTTCAAAGG
TGCTGATGATCGTAAGGAAGCTGCAGATCATTCACTTAAGGCTTACGAGG
CTGCTACAGCTGCAGCAGCCTCAGAGTTACCCCCAACTCATCCAATCAGA
CTTGGCCTGGCTCTTAACTTTTCTGTTTTCTACTATGAGATTTTGAACTC
ACCTGAGAGGGCTTGCCACCTGGCTAAACAGCATTTGATGAGGCGATTGC
TGAACTTGATAGCCTTAATG
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